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 Charlie Jeynes

Charlie Jeynes

STFC 'innovation' Research Fellow

 7471

 01392 727471

 Living Systems Institute Level 3, Room T03.02

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

Please see my lab website.

Dr Charlie Jeynes is an STFC 'Innovation' Research Fellow working at the Centre for Biomedical Modelling and Analysis, University of Exeter, U.K. This centre is an Institutional Strategic Support Fund, with the aim of developing computational analysis and mathematical modelling within the life sciences.   

After studying for a degree in Biochemistry and Genetics at the University of Wales, Cardiff, he moved to the University of Surrey where he was awarded a PhD functionalising carbon nanotubes with DNA.

He then worked as a Post-Doc for the CONFORM consortium, a collaboration between a number of institutions including the Universities of Oxford/Surrey/Manchester and the Cockcroft Institute, investigating next generation particle accelerators largely for cancer treatment.

Following this, he worked as the scientific officer for the U.K. National Ion Beam Centre, facilitating beam access for various institutions, as well as pursuing his own research interests into nanoparticle enhanced radiation therapies.

He then moved to the University of Reading where he completed his Higher Education associate fellow training (AFHFA) as a Teaching Fellow.

While at the University of Exeter, he has worked as an independant Wellcome Trust Fellow at the Centre for Biomedical Modelling and Analysis, working with a range of academics including Prof Christian Soeller and Prof John Terry - largely on correlative single molecule imaging technologies using nanoparticles.

He was recently awarded an STFC 'Innovation' Research Fellowship working with http://emps.exeter.ac.uk/physics-astronomy/staff/tjharrie Tim Harries to pursue modelling and experimental techniques to investigate how nanoparticles can be used in Photodynamic Therapy. 

Broad research specialisms:

  • X-ray fluorescence microscopy
  • Single Molecule Imaging
  • Image Processing
  • Radiation Biology
  • Nanotoxicology
  • Nanotechnology

Qualifications

2015 Associate Fellow Higher Education Academy
2003-2007 Ph.D, Bio-nanotechnology, University of Surrey
1999-2003 B.Sc, Genetics, University of Cardiff (year in industry Merck KgA)

Career

07/2015-present

University of Exeter – STFC ‘Innovation’ Research Fellow –

A 3 year Fellowship, in collaboration with Prof Tim Harries (UoE), to continue my research in metal nanoparticle probes for cancer photodynamic therapy

06/2015-07/2018

University of Exeter – Wellcome Trust ISSF Research Fellow –

A highly competitive award with £10K consumables to pursue my own research interests; supported by the Institution Science Support Funded (ISSF) Centre of Biomedical Modelling and Analysis; 50% of my time was spent using my skills in quantitative imaging with academics in Exeter ad beyond.

07/2014-06/2015

University of Reading – Teaching Fellow

I lectured large (>300) and small (<5) groups mainly biochemistry related modules. I also obtained my Higher Education Award.

06/2012-06/2014

UK National Ion Beam Centre (Surrey) – Scientific Experimental Officer

My role was to facilitate experiments from a range of scientists on the proton induced X-ray fluorescent imaging spectroscopy beamline 

07/2007-06/2012

CONFORM project (Universities of Oxford, Surrey & Cockcroft Institute)

Post-doc position where I developed a proton beamline capable of irradiating and analysing human tissue – for applications in proton cancer therapy

06/2001-06/2002

Merck KgA, Newport, UK (industrial placement)

Research

Research interests

The broad theme of my work is to investigate nanoparticles for human health. This includes studying the toxic effect of nanoparticles from air pollution; using nanoparticles to develop new tools to probe biological processes on a single molecule level; as well as developing novel drugs based on nanoparticle designs.

Key to my research is using analytical imaging methods to quantify nanoparticles in human cells and tissue.

I use a range of imaging techniques including electron microscopy, chemical imaging (Infra-Red/Raman), X-ray fluorescence microscopy (see IBC, Diamond Synchrotron) optical super resolution microscopy and fluorescent imaging.

I am also involved in a range of “seed corn” projects with academics at the University of Exeter supported through the Centre for Biomedical Modelling and Analysis. These projects involve utilising my analytical imaging skills for novel applications.

Research projects

Please see my lab website for current/past research projects.

Grants/funding

06/2018-08/2018

BBSRC-Research Experience Placement student (2.5K, I was awarded this to host a student at UoE to assist me in advancing qCLEM probes, and for their own career development, with myself as Principle Supervisor)

03/2017-present

Science and Technologies Facilities Council (£6K, A workshop to bring together simulation experts, image specialist and experimentalists to develop multi-scale models in biology, Queen’s Belfast April 2018)

02/2017-present

EPSRC (£9.8K, beamtime funding for UK National Ion Beam Centre [IBC])

The IBC is an EPSRC medium scale facility offering competitive beamtime access (PI with F.Palombo, Exeter)

09/2016-09/2017

Royal Society (£3K, Partnership Award - see outreach section) 

03/2016-092016

Royal Society (£3K, International Exchange Award)

This was a personal award to visit my collaborator Prof Andrew Bettiol @ National University of Singapore to work on X-ray nano-imaging 

02/201607-2016

Diamond Lightsource Synchrotron (‘in kind’ £10K Beamtime Access Award)

Highly competitive beamtime access granted through peer review & panel

09/2015-09/2016

Royal Society of Chemistry (£1K, Partnership Award – see outreach section)

06/2015-present

Wellcome Trust ‘Seed Corn’ ISSF Award (£10K)

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Jeynes JCG, Geraki K, Jeynes C, Zhaohong M, Bettiol AA, Latorre E, Harries LW, Soeller C (In Press). Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe. ACS Nano, 11(12), 12632-12640. Abstract.  Author URL.  Full text.
Latorre E, Birar VC, Sheerin AN, Jeynes JCC, Hooper A, Dawe HR, Melzer D, Cox LS, Faragher RGA, Ostler EL, et al (In Press). Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence. BMC Cell Biol, 18(1). Abstract.  Author URL.  Full text.
Palombo F, Tamagnini F, Jeynes J, Mattana S, Swift I, Nallala J, Hancock J, Brown J, Randall A, Stone N, et al (2018). Detection of Aβ plaque-associated astrogliosis in Alzheimer’s disease brain by spectroscopic imaging and immunohistochemistry. Analyst, 143, 850-857. Full text.
Georgantzoglou A, Merchant MJ, Jeynes JCG, Mayhead N, Punia N, Butler RE, Jena R (2016). Applications of high-throughput clonogenic survival assays in high-LET particle microbeams. Frontiers in Oncology, 5(JAN). Abstract.
Jeynes JCG, Jeynes C, Palitsin V, Townley HE (2016). Direct quantification of rare earth doped titania nanoparticles in individual human cells. NANOTECHNOLOGY, 27(28). Author URL.
Georgantzoglou A, Merchant MJ, Jeynes JCG, Wera A-C, Kirkby KJ, Kirkby NF, Jena R (2015). Automatic cell detection in bright-field microscopy for microbeam irradiation studies. PHYSICS IN MEDICINE AND BIOLOGY, 60(16), 6289-6303. Author URL.
Barazzuol L, Jeynes JCG, Merchant MJ, Wera A-C, Barry MA, Kirkby KJ, Suzuki M (2015). Radiosensitization of glioblastoma cells using a histone deacetylase inhibitor (SAHA) comparing carbon ions with X-rays. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 91(1), 90-98. Author URL.
Mohamad AS, Jeynes JCG, Hughes MP (2014). Dielectrophoretic Response of DNA Shows Different Conduction Mechanisms for Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) in Solution. IEEE TRANSACTIONS ON NANOBIOSCIENCE, 13(1), 51-54. Author URL.
Wera A-C, Barazzuol L, Jeynes JCG, Merchant MJ, Suzuki M, Kirkby KJ (2014). Influence of the nucleus area distribution on the survival fraction after charged particles broad beam irradiation. PHYSICS IN MEDICINE AND BIOLOGY, 59(15), 4197-4211. Author URL.
Jeynes JCG, Merchant MJ, Spindler A, Wera A-C, Kirkby KJ (2014). Investigation of gold nanoparticle radiosensitization mechanisms using a free radical scavenger and protons of different energies. PHYSICS IN MEDICINE AND BIOLOGY, 59(21), 6431-6443. Author URL.
Jeynes JCG, Merchant MJ, Barazzuol L, Barry M, Guest D, Palitsin VV, Grime GW, Tullis IDC, Barber PR, Vojnovic B, et al (2013). "Broadbeam" irradiation of mammalian cells using a vertical microbeam facility. RADIATION AND ENVIRONMENTAL BIOPHYSICS, 52(4), 513-521. Author URL.
Barazzuol L, Jena R, Burnet NG, Meira LB, Jeynes JCG, Kirkby KJ, Kirkby NF (2013). Evaluation of poly (ADP-ribose) polymerase inhibitor ABT-888 combined with radiotherapy and temozolomide in glioblastoma. RADIATION ONCOLOGY, 8 Author URL.
Jeynes JCG, Jeynes C, Merchant MJ, Kirkby KJ (2013). Measuring and modelling cell-to-cell variation in uptake of gold nanoparticles. ANALYST, 138(23), 7070-7074. Author URL.
Merchant MJ, Jeynes JCG, Grime GW, Palitsin V, Tullis IDW, Barber PR, Vojnovic B, Webb RP, Kirkby KJ (2012). A Focused Scanning Vertical Beam for Charged Particle Irradiation of Living Cells with Single Counted Particles. RADIATION RESEARCH, 178(3), 182-190. Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological effectiveness on live cells of laser driven protons at dose rates exceeding 10(9) Gy/s. AIP ADVANCES, 2(1). Author URL.
Barazzuol L, Jena R, Burnet NG, Jeynes JCG, Merchant MJ, Kirkby KJ, Kirkby NF (2012). In Vitro Evaluation of Combined Temozolomide and Radiotherapy Using X Rays and High-Linear Energy Transfer Radiation for Glioblastoma. RADIATION RESEARCH, 177(5), 651-662. Author URL.
Fiorini F, Kirby D, Borghesi M, Doria D, Jeynes JCG, Kakolee KF, Kar S, Kaur S, Kirby KJ, Merchant MJ, et al (2011). Dosimetry and spectral analysis of a radiobiological experiment using laser-driven proton beams. PHYSICS IN MEDICINE AND BIOLOGY, 56(21), 6969-6982. Author URL.
Jeynes JCG, Webb M, Kirkby KJ, Kirkby NF, Bradley DA (2009). Proceedings of the First International Conference on Biomedical Applications of High Energy Ion Beams Preface. APPLIED RADIATION AND ISOTOPES, 67(3), 369-370. Author URL.
Jeynes JCG, Mendoza E, Chow DCS, Watts PCR, McFadden J, Silva SRP (2006). Generation of chemically unmodified pure single-walled carbon nanotubes by solubilizing with RNA and treatment with ribonuclease A. ADVANCED MATERIALS, 18(12), 1598-+. Author URL.
Newcombe J, Jeynes JC, Mendoza E, Hinds J, Marsden GL, Stabler RA, Marti M, McFadden JJ (2005). Phenotypic and transcriptional characterization of the meningococcal PhoPQ system, a magnesium-sensing two-component regulatory system that controls genes involved in remodeling the meningococcal cell surface. J Bacteriol, 187(14), 4967-4975. Abstract.  Author URL.

Conferences

Kar S, Doria D, Kakolee KF, Prasad R, Litt S, Ahmed H, Nersisyan G, Lewis C, Zepf M, Borghesi M, et al (2013). First Results on Cell Irradiation with laser-driven protons on the TARANIS system.  Author URL.
Prakrajang K, Jeynes JCG, Merchant MJ, Kirkby K, Kirkby N, Thopan P, Yu LD (2013). MeV single-ion beam irradiation of mammalian cells using the Surrey vertical nanobeam, compared with broad proton beam and X-ray irradiations.  Author URL.
Hanton F, Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, et al (2013). Radiobiology at ultra-high dose rates employing laser-driven ions.  Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological Cell Irradiation at Ultrahigh Dose Rate Employing Laser Driven Protons.  Author URL.
Jeynes JCG, Bailey MJ, Coley H, Kirkby KJ, Jeynes C (2010). Microbeam PIXE analysis of platinum resistant and sensitive ovarian cancer cells.  Author URL.
Jeynes JCG, Jeynes C, Kirkby KJ, Ruemmeli A, Silva SRP (2008). RBS/EBS/PIXE measurement of single-walled carbon nanotube modification by nitric acid purification treatment.  Author URL.
Jeynes JCG, Mendoza E, Chow D, McFadden J, Silva SRP (2005). DNA/RNA purified single walled carbon nanotubes on self-assembled networks.  Abstract.

Publications by year


In Press

Jeynes JCG, Geraki K, Jeynes C, Zhaohong M, Bettiol AA, Latorre E, Harries LW, Soeller C (In Press). Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe. ACS Nano, 11(12), 12632-12640. Abstract.  Author URL.  Full text.
Latorre E, Birar VC, Sheerin AN, Jeynes JCC, Hooper A, Dawe HR, Melzer D, Cox LS, Faragher RGA, Ostler EL, et al (In Press). Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence. BMC Cell Biol, 18(1). Abstract.  Author URL.  Full text.

2018

Palombo F, Tamagnini F, Jeynes J, Mattana S, Swift I, Nallala J, Hancock J, Brown J, Randall A, Stone N, et al (2018). Detection of Aβ plaque-associated astrogliosis in Alzheimer’s disease brain by spectroscopic imaging and immunohistochemistry. Analyst, 143, 850-857. Full text.

2016

Georgantzoglou A, Merchant MJ, Jeynes JCG, Mayhead N, Punia N, Butler RE, Jena R (2016). Applications of high-throughput clonogenic survival assays in high-LET particle microbeams. Frontiers in Oncology, 5(JAN). Abstract.
Jeynes JCG, Jeynes C, Palitsin V, Townley HE (2016). Direct quantification of rare earth doped titania nanoparticles in individual human cells. NANOTECHNOLOGY, 27(28). Author URL.

2015

Georgantzoglou A, Merchant MJ, Jeynes JCG, Wera A-C, Kirkby KJ, Kirkby NF, Jena R (2015). Automatic cell detection in bright-field microscopy for microbeam irradiation studies. PHYSICS IN MEDICINE AND BIOLOGY, 60(16), 6289-6303. Author URL.
Barazzuol L, Jeynes JCG, Merchant MJ, Wera A-C, Barry MA, Kirkby KJ, Suzuki M (2015). Radiosensitization of glioblastoma cells using a histone deacetylase inhibitor (SAHA) comparing carbon ions with X-rays. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 91(1), 90-98. Author URL.

2014

Mohamad AS, Jeynes JCG, Hughes MP (2014). Dielectrophoretic Response of DNA Shows Different Conduction Mechanisms for Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) in Solution. IEEE TRANSACTIONS ON NANOBIOSCIENCE, 13(1), 51-54. Author URL.
Wera A-C, Barazzuol L, Jeynes JCG, Merchant MJ, Suzuki M, Kirkby KJ (2014). Influence of the nucleus area distribution on the survival fraction after charged particles broad beam irradiation. PHYSICS IN MEDICINE AND BIOLOGY, 59(15), 4197-4211. Author URL.
Jeynes JCG, Merchant MJ, Spindler A, Wera A-C, Kirkby KJ (2014). Investigation of gold nanoparticle radiosensitization mechanisms using a free radical scavenger and protons of different energies. PHYSICS IN MEDICINE AND BIOLOGY, 59(21), 6431-6443. Author URL.

2013

Jeynes JCG, Merchant MJ, Barazzuol L, Barry M, Guest D, Palitsin VV, Grime GW, Tullis IDC, Barber PR, Vojnovic B, et al (2013). "Broadbeam" irradiation of mammalian cells using a vertical microbeam facility. RADIATION AND ENVIRONMENTAL BIOPHYSICS, 52(4), 513-521. Author URL.
Barazzuol L, Jena R, Burnet NG, Meira LB, Jeynes JCG, Kirkby KJ, Kirkby NF (2013). Evaluation of poly (ADP-ribose) polymerase inhibitor ABT-888 combined with radiotherapy and temozolomide in glioblastoma. RADIATION ONCOLOGY, 8 Author URL.
Kar S, Doria D, Kakolee KF, Prasad R, Litt S, Ahmed H, Nersisyan G, Lewis C, Zepf M, Borghesi M, et al (2013). First Results on Cell Irradiation with laser-driven protons on the TARANIS system.  Author URL.
Prakrajang K, Jeynes JCG, Merchant MJ, Kirkby K, Kirkby N, Thopan P, Yu LD (2013). MeV single-ion beam irradiation of mammalian cells using the Surrey vertical nanobeam, compared with broad proton beam and X-ray irradiations.  Author URL.
Jeynes JCG, Jeynes C, Merchant MJ, Kirkby KJ (2013). Measuring and modelling cell-to-cell variation in uptake of gold nanoparticles. ANALYST, 138(23), 7070-7074. Author URL.
Hanton F, Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, et al (2013). Radiobiology at ultra-high dose rates employing laser-driven ions.  Author URL.

2012

Merchant MJ, Jeynes JCG, Grime GW, Palitsin V, Tullis IDW, Barber PR, Vojnovic B, Webb RP, Kirkby KJ (2012). A Focused Scanning Vertical Beam for Charged Particle Irradiation of Living Cells with Single Counted Particles. RADIATION RESEARCH, 178(3), 182-190. Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological Cell Irradiation at Ultrahigh Dose Rate Employing Laser Driven Protons.  Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological effectiveness on live cells of laser driven protons at dose rates exceeding 10(9) Gy/s. AIP ADVANCES, 2(1). Author URL.
Barazzuol L, Jena R, Burnet NG, Jeynes JCG, Merchant MJ, Kirkby KJ, Kirkby NF (2012). In Vitro Evaluation of Combined Temozolomide and Radiotherapy Using X Rays and High-Linear Energy Transfer Radiation for Glioblastoma. RADIATION RESEARCH, 177(5), 651-662. Author URL.

2011

Fiorini F, Kirby D, Borghesi M, Doria D, Jeynes JCG, Kakolee KF, Kar S, Kaur S, Kirby KJ, Merchant MJ, et al (2011). Dosimetry and spectral analysis of a radiobiological experiment using laser-driven proton beams. PHYSICS IN MEDICINE AND BIOLOGY, 56(21), 6969-6982. Author URL.

2010

Jeynes JCG, Bailey MJ, Coley H, Kirkby KJ, Jeynes C (2010). Microbeam PIXE analysis of platinum resistant and sensitive ovarian cancer cells.  Author URL.

2009

Jeynes JCG, Webb M, Kirkby KJ, Kirkby NF, Bradley DA (2009). Proceedings of the First International Conference on Biomedical Applications of High Energy Ion Beams Preface. APPLIED RADIATION AND ISOTOPES, 67(3), 369-370. Author URL.

2008

Jeynes JCG, Jeynes C, Kirkby KJ, Ruemmeli A, Silva SRP (2008). RBS/EBS/PIXE measurement of single-walled carbon nanotube modification by nitric acid purification treatment.  Author URL.

2006

Jeynes JCG, Mendoza E, Chow DCS, Watts PCR, McFadden J, Silva SRP (2006). Generation of chemically unmodified pure single-walled carbon nanotubes by solubilizing with RNA and treatment with ribonuclease A. ADVANCED MATERIALS, 18(12), 1598-+. Author URL.

2005

Jeynes JCG, Mendoza E, Chow D, McFadden J, Silva SRP (2005). DNA/RNA purified single walled carbon nanotubes on self-assembled networks.  Abstract.
Newcombe J, Jeynes JC, Mendoza E, Hinds J, Marsden GL, Stabler RA, Marti M, McFadden JJ (2005). Phenotypic and transcriptional characterization of the meningococcal PhoPQ system, a magnesium-sensing two-component regulatory system that controls genes involved in remodeling the meningococcal cell surface. J Bacteriol, 187(14), 4967-4975. Abstract.  Author URL.

Charlie_Jeynes Details from cache as at 2018-12-09 23:53:43

Refresh publications

External Engagment and Impact

Editorial responsibilities

Reviewer for a number of journals including Nanotechnology, Chemosphere and PLOS one. 



Invited lectures

June 2018:Conference talk at South West Structural Biology Conference 2018, Exeter“Dual-purpose gold nanoparticle probes for quantification in structural biology”

September 2017:Conference talk at Recent Appointments in Material Science Conference 2017, Exeter“Constructing quantitative X-ray fluorescence super resolution gold nanoparticle probes to measure biophysical properties of human chromosomes”

March 2017:Invited talk at Monte Carlo Multi-scale Modelling in Radiotherapy: From Ionisation to Clinical Impact, 2017 Manchester “Aligning experiments and models; commercial software symposium”

September 2016:Conference talk at BioDynamics Workshop 2016, Exeter“Measuring the length of human telomeres with XRF to inform cell ageing models”

July 2016:Conference talk at 16thIUBMB Conference, Vancouver, Canada “Getting more from QFISH: Correlation of Super Resolution Microscopy and X-ray Spectroscopy on nanoparticle probed telomeres”

June 2016:Invited talk at Department of Physics, National University of Singapore “Correlation of Super Resolution and X-ray Spectroscopy on nanoparticle probed telomeres”

September 2014:Invited talk at 8thInternational Symposium on BioPIXE, Slovenia “Measuring and modelling gold nanoparticles uptake in individual cancer cells with X-ray fluorescence”

Teaching

I am currently supervising with course lead James Wakefield, and Christian Soeller, a number of 3rd year undergraduate projects on the Natural Sciences degree.

PhD Students (PG researchers):

I am currently involved in a Doctoral Training Centre associated with CBMA  and co-supervising a number of PhD students.

Supervision / Group

Postgraduate researchers

  • I am currently involved in a Doctoral Training Centre associated with CBMA and co-supervising and helping a number of Ph.D students.

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