Professor Jennifer Littlechild
Professor of Biological Chemistry

Key publications | Publications by category | Publications by year

Key publications



Fraser CS, Rubio-Cabezas O, Littlechild JA, Ellard S, Hattersley AT, Flanagan SE (2012). Amino acid properties may be useful in predicting clinical outcome in patients with Kir6.2 neonatal diabetes. Eur J Endocrinol, 167(3), 417-421. Abstract.  Author URL. DOI.
Sayer C, Bommer M, Isupov M, Ward J, Littlechild J (2012). Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus. Acta Crystallographica Section D: Biological Crystallography, 68(7), 763-772. Abstract.  Full text. DOI.
Hickey AM, Ngamsom B, Wiles C, Greenway GM, Watts P, Littlechild JA (2009). A microreactor for the study of biotransformations by a cross-linked gamma-lactamase enzyme. Biotechnol J, 4(4), 510-516. Abstract.  Author URL. DOI.
Willies SC, Isupov MN, Garman EF, Littlechild JA (2009). The binding of haem and zinc in the 1.9 a X-ray structure of Escherichia coli bacterioferritin. J Biol Inorg Chem, 14(2), 201-207. Abstract.  Author URL.  Full text. DOI.
Littlechild J, Garcia Rodriguez E, Isupov M (2009). Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography. J Inorg Biochem, 103(4), 617-621. Abstract.  Author URL.  Full text. DOI.
Line K, Isupov MN, Garcia-Rodriguez E, Maggioli G, Parra F, Littlechild JA (2008). The Fasciola hepatica thioredoxin: High resolution structure reveals two oxidation states. Mol Biochem Parasitol, 161(1), 44-48. Abstract.  Author URL.  Full text. DOI.

Publications by category


Books

Satyanarayana T, Littlechild J, Kawarabayasi Y (2013). Preface. DOI.

Journal articles

Littlechild JA (In Press). Improving the ‘Tool Box’ for Robust Industrial Enzymes. Journal of Industrial Microbiology & Biotechnology Full text.
Antranikian G, Suleiman M, Schäfers C, Adams MWW, Bartolucci S, Blamey JM, Birkeland N-K, Bonch-Osmolovskaya E, da Costa MS, Cowan D, et al (2017). Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island. Extremophiles, 21(4), 733-742. Abstract.  Author URL. DOI.
de Rose SA, Novak H, Dowd A, Singh S, Lang DA, Littlechild J (2017). Stabilization of a lipolytic enzyme for commercial application. Catalysts, 7(3). Abstract. DOI.
Finnigan W, Thomas A, Cromar H, Gough B, Snajdrova R, Adams JP, Littlechild JA, Harmer NJ (2016). Characterization of carboxylic acid reductases as enzymes in the toolbox for synthetic chemistry. ChemCatChem, in press Full text. DOI.
Zarafeta D, Kissas D, Sayer C, Gudbergsdottir SR, Ladoukakis E, Isupov MN, Chatziioannou A, Peng X, Littlechild JA, Skretas G, et al (2016). Discovery and Characterization of a Thermostable and Highly Halotolerant GH5 Cellulase from an Icelandic Hot Spring Isolate. PLoS One, 11(1). Abstract.  Author URL.  Full text. DOI.
sayer C, Finnigan W, Isupov MN, Levisson M, Kengen SWM, van der Oost J, Harmer NJ, Littlechild JA (2016). Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site. Scientific Reports, 6, 25542-25542. Abstract.  Full text. DOI.
Littlechild JA (2015). Archaeal Enzymes and Applications in Industrial Biocatalysts. Archaea, 2015, 1-10. Full text. DOI.
Ferrandi EE, Sayer C, Isupov MN, Annovazzi C, Marchesi C, Iacobone G, Peng X, Bonch-Osmolovskaya E, Wohlgemuth R, Littlechild JA, et al (2015). Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries. FEBS J, 282(15), 2879-2894. Abstract.  Author URL. DOI.
Littlechild JA (2015). Enzymes from Extreme Environments and Their Industrial Applications. Front Bioeng Biotechnol, 3 Abstract.  Author URL.  Full text. DOI.
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from a new Planctomycetes species, Thermogutta terrifontis. FEBS J, 282(15), 2846-2857. Abstract.  Author URL. DOI.
Sayer C, Szabo Z, Isupov MN, Ingham C, Littlechild JA (2015). The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain. Front Microbiol, 6 Abstract.  Author URL.  Full text. DOI.
Isupov MN, Schröder E, Gibson RP, Beecher J, Donadio G, Saneei V, Dcunha SA, McGhie EJ, Sayer C, Davenport CF, et al (2015). The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of a type II Baeyer-Villiger monooxygenase. Acta Crystallogr D Biol Crystallogr, 71(Pt 11), 2344-2353. Abstract.  Author URL.  Full text. DOI.
Novak HR, Sayer C, Isupov MN, Gotz D, Spragg AM, Littlechild JA (2014). Biochemical and structural characterisation of a haloalkane dehalogenase from a marine Rhodobacteraceae. FEBS Lett, 588(9), 1616-1622. Abstract.  Author URL.  Full text. DOI.
Kallnik V, Bunescu A, Sayer C, Bräsen C, Wohlgemuth R, Littlechild J, Siebers B (2014). Characterization of a phosphotriesterase-like lactonase from the hyperthermoacidophilic crenarchaeon Vulcanisaeta moutnovskia. J Biotechnol, 190, 11-17. Abstract.  Author URL. DOI.
Vivoli M, Novak HR, Littlechild JA, Harmer NJ (2014). Determination of protein-ligand interactions using differential scanning fluorimetry. J Vis Exp(91). Abstract.  Author URL.  Full text. DOI.
Littlechild J, Sayer C, Isupov M, Ward J, Littlechild J (2014). Structural studies on transaminase enzymes and applications in biocatalysis. NEW BIOTECHNOLOGY, 31, S19-S19. Author URL. DOI.
James P, Isupov MN, Sayer C, Saneei V, Berg S, Lioliou M, Kotlar HK, Littlechild JA (2014). The structure of a tetrameric α-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability. Acta Crystallogr D Biol Crystallogr, 70(Pt 10), 2607-2618. Abstract.  Author URL.  Full text. DOI.
Sayer C, Martinez-Torres RJ, Richter N, Isupov MN, Hailes HC, Littlechild JA, Ward JM (2014). The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca. FEBS J, 281(9), 2240-2253. Abstract.  Author URL.  Full text. DOI.
Holley JE, Slonka J, Littlechild J, Gutowski NJ (2013). An investigation into the presence of pyroglutamyl peptidases in the Alzheimer's disease brain: relevance to pathology?. JOURNAL OF NEUROLOGY, 260, S167-S167. Author URL.
Novak HR, Sayer C, Panning J, Littlechild JA (2013). Characterisation of an L-haloacid dehalogenase from the marine psychrophile Psychromonas ingrahamii with potential industrial application. Mar Biotechnol (NY), 15(6), 695-705. Abstract.  Author URL. DOI.
Novak HR, Sayer C, Panning J, Littlechild JA (2013). Characterisation of an l-Haloacid Dehalogenase from the Marine Psychrophile Psychromonas ingrahamii with Potential Industrial Application. Marine Biotechnology, 15(6), 695-705. Abstract. DOI.
Reid EL, Weynberg KD, Love J, Isupov MN, Littlechild JA, Wilson WH, Kelly SL, Lamb DC, Allen MJ (2013). Functional and structural characterisation of a viral cytochrome b5. FEBS Lett, 587(22), 3633-3639. Abstract.  Author URL. DOI.
Novak HR, Sayer C, Isupov MN, Paszkiewicz K, Gotz D, Spragg AM, Littlechild JA (2013). Marine Rhodobacteraceae L-haloacid dehalogenase contains a novel His/Glu dyad that could activate the catalytic water. FEBS J, 280(7), 1664-1680. Abstract.  Author URL.  Full text. DOI.
Novak H, Littlechild J (2013). Marine enzymes with applications for biosynthesis of fine chemicals. , 89-106. Abstract. DOI.
Littlechild JA (2013). Protein structure and function. , 57-79. Abstract. DOI.
Sayer C, Isupov MN, Westlake A, Littlechild JA (2013). Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity. Acta Crystallogr D Biol Crystallogr, 69(Pt 4), 564-576. Abstract.  Author URL.  Full text. DOI.
Hill A, Littlechild J (2012). 7.15 Oxidation: Haloperoxidases. , 7, 329-349. Abstract. DOI.
Fraser CS, Rubio-Cabezas O, Littlechild JA, Ellard S, Hattersley AT, Flanagan SE (2012). Amino acid properties may be useful in predicting clinical outcome in patients with Kir6.2 neonatal diabetes. Eur J Endocrinol, 167(3), 417-421. Abstract.  Author URL. DOI.
Sayer C, Bommer M, Isupov M, Ward J, Littlechild J (2012). Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus. Acta Crystallographica Section D: Biological Crystallography, 68(7), 763-772. Abstract.  Full text. DOI.
Szabo-Taylor KE, Eggleton P, Turner CAL, Lo Faro ML, Tarr JM, Toth S, Whiteman M, Haigh RC, Littlechild JA, Winyard PG, et al (2012). Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 44(8), 1223-1231. Author URL. DOI.
Reid EL, Worthy CA, Probert I, Ali ST, Love J, Napier J, Littlechild JA, Somerfield PJ, Allen MJ (2011). Coccolithophores: functional biodiversity, enzymes and bioprospecting. Mar Drugs, 9(4), 586-602. Abstract.  Author URL.  Full text. DOI.
Littlechild JA (2011). Thermophilic archaeal enzymes and applications in biocatalysis. Biochem Soc Trans, 39(1), 155-158. Abstract.  Author URL. DOI.
Wilson RA, Gibson RP, Quispe CF, Littlechild JA, Talbot NJ (2010). An NADPH-dependent genetic switch regulates plant infection by the rice blast fungus. Proc Natl Acad Sci U S A, 107(50), 21902-21907. Abstract.  Author URL. DOI.
Opperman DJ, Sewell BT, Litthauer D, Isupov MN, Littlechild JA, van Heerden E (2010). Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01. Biochem Biophys Res Commun, 393(3), 426-431. Abstract.  Author URL. DOI.
Ngamsom B, Hickey AM, Greenway GM, Littlechild JA, Watts P, Wiles C (2010). Development of a high throughput screening tool for biotransformations utilising a thermophilic l-aminoacylase enzyme. Journal of Molecular Catalysis B: Enzymatic, 63(1-2), 81-86. Abstract. DOI.
Ngamsom B, Hickey AM, Greenway GM, Littlechild JA, McCreedy T, Watts P, Wiles C (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Org Biomol Chem, 8(10), 2419-2424. Abstract.  Author URL. DOI.
Willies S, Isupov M, Littlechild J (2010). Thermophilic enzymes and their applications in biocatalysis: a robust aldo-keto reductase. Environ Technol, 31(10), 1159-1167. Abstract.  Author URL. DOI.
Hickey AM, Ngamsom B, Wiles C, Greenway GM, Watts P, Littlechild JA (2009). A microreactor for the study of biotransformations by a cross-linked gamma-lactamase enzyme. Biotechnol J, 4(4), 510-516. Abstract.  Author URL. DOI.
Rye CA, Isupov MN, Lebedev AA, Littlechild JA (2009). Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii. Extremophiles, 13(1), 179-190. Abstract.  Author URL.  Full text. DOI.
Willies SC, Isupov MN, Garman EF, Littlechild JA (2009). The binding of haem and zinc in the 1.9 a X-ray structure of Escherichia coli bacterioferritin. J Biol Inorg Chem, 14(2), 201-207. Abstract.  Author URL.  Full text. DOI.
Littlechild J (2009). Thermophilic enzymes as stable biocatalysts. NEW BIOTECHNOLOGY, 25, S108-S108. Author URL. DOI.
Littlechild J, Garcia Rodriguez E, Isupov M (2009). Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography. J Inorg Biochem, 103(4), 617-621. Abstract.  Author URL.  Full text. DOI.
Littlechild J (2008). Catalyst for improvement. EBR - European Biopharmaceutical Review(SUMM), 27-32. Abstract.
Ngamsom B, Marle L, Hickey AM, Greenway GM, Watts P, McCreedy T, Littlechild JA (2008). Immobilisation of thermophilic enzymes within miniaturised flow reactors for biotransformations. AIChE Annual Meeting, Conference Proceedings
Line K, Isupov MN, Garcia-Rodriguez E, Maggioli G, Parra F, Littlechild JA (2008). The Fasciola hepatica thioredoxin: High resolution structure reveals two oxidation states. Mol Biochem Parasitol, 161(1), 44-48. Abstract.  Author URL.  Full text. DOI.
Rye CA, Isupov MN, Lebedev AA, Littlechild JA (2007). An order-disorder twin crystal of L-2-haloacid dehalogenase from Sulfolobus tokodaii. Acta Crystallogr D Biol Crystallogr, 63(Pt 8), 926-930. Abstract.  Author URL.  Full text. DOI.
Battershill C, Bavington C, Chahal S, Jaspars M, Littlechild J, Spragg AM (2007). Contributions of marine bioscience to industrial biotechology. Industrial Biotechnology, 3(4), 304-313. Abstract. DOI.
Sayer C, Isupov MN, Littlechild JA (2007). Crystallization and preliminary X-ray diffraction analysis of omega-amino acid:pyruvate transaminase from Chromobacterium violaceum. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63(Pt 2), 117-119. Abstract.  Author URL.  Full text. DOI.
Hickey AM, Marle L, McCreedy T, Watts P, Greenway GM, Littlechild JA (2007). Immobilization of thermophilic enzymes in miniaturized flow reactors. Biochem Soc Trans, 35(Pt 6), 1621-1623. Abstract.  Author URL. DOI.
Littlechild JA, Guy J, Connelly S, Mallett L, Waddell S, Rye CA, Line K, Isupov M (2007). Natural methods of protein stabilization: thermostable biocatalysts. Biochem Soc Trans, 35(Pt 6), 1558-1563. Abstract.  Author URL. DOI.
Littlechild J (2007). Nature's catalysts for biosynthesis of natural products. AMINO ACIDS, 33(3), XIV-XIV. Author URL.
Watts P, McCreedy T, Marle L, Littlechild J, Hickey AM, Greenway GM (2007). ORGN 104-The use of immobilized thermophilic enzymes for organic synthesis in flow reactors. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 234 Author URL.
Coupe EE, Smyth MG, Fosberry AP, Hall RM, Littlechild JA (2007). The dodecameric vanadium-dependent haloperoxidase from the marine algae Corallina officinalis: cloning, expression, and refolding of the recombinant enzyme. Protein Expr Purif, 52(2), 265-272. Abstract.  Author URL. DOI.
Talbot NJ, Littlechild JA, Wang Z-Y, Wilson RA (2007). Tps1 regulates the pentose phosphate pathway, nitrogen metabolism and fungal virulence. The EMBO Journal, 26, 3673-3685. DOI.
Wilson RA, Jenkinson JM, Gibson RP, Littlechild JA, Wang Z-Y, Talbot NJ (2007). Tps1 regulates the pentose phosphate pathway, nitrogen metabolism and fungal virulence. EMBO J, 26(15), 3673-3685. Abstract.  Author URL. DOI.
Tapper S, Littlechild JA, Molard Y, Prokes I, Tucker JHR (2006). Anion binding tripodal receptors as structural models for the active site of vanadium haloperoxidases and acid phosphatases. Supramolecular Chemistry, 18(1), 55-58. Abstract. DOI.
Conklin PL, Gatzek S, Wheeler GL, Dowdle J, Raymond MJ, Rolinski S, Isupov M, Littlechild JA, Smirnoff N (2006). Arabidopsis thaliana VTC4 encodes L-galactose-1-P phosphatase, a plant ascorbic acid biosynthetic enzyme. J Biol Chem, 281(23), 15662-15670. Abstract.  Author URL. DOI.
Littlechild J, Garcia-Rodriguez E, Isupov M (2006). INOR 398-Crystallization studies of vanadium haloperoxidases: Insights into halide specificity and enzyme mechanism. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 232 Author URL.
Garcia-Rodriguez E, Ohshiro T, Aibara T, Izumi Y, Littlechild J (2005). Enhancing effect of calcium and vanadium ions on thermal stability of bromoperoxidase from Corallina pilulifera. J Biol Inorg Chem, 10(3), 275-282. Abstract.  Author URL. DOI.
Littlechild J (2005). Nature's catalysts and their applications in the pharmaceutical industry. EBR - European Biopharmaceutical Review(AUTUMN), 64-68. Abstract.
Connelly S, Line K, Isupov MN, Littlechild JA (2005). Synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the Aureobacterium species (-) gamma-lactamase. Org Biomol Chem, 3(18), 3260-3262. Abstract.  Author URL. DOI.
Clark DS, Ball P, Littlechild JA, Rice DW, Halle B (2004). Characteristics of nearly dry enzymes in organic solvents: Implications for biocatalysis in the absence of water. Philosophical Transactions of the Royal Society B: Biological Sciences, 359(1448), 1299-1307. Abstract. DOI.
Brice DC, Bryant JA, Dambrauskas G, Drury SC, Littlechild JA (2004). Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.). J Exp Bot, 55(398), 955-956. Abstract.  Author URL. DOI.
Isupov MN, Brindley AA, Hollingsworth EJ, Murshudov GN, Vagin AA, Littlechild JA (2004). Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. Acta Crystallogr D Biol Crystallogr, 60(Pt 10), 1879-1882. Abstract.  Author URL.  Full text. DOI.
Ohshiro T, Littlechild J, Garcia-Rodriguez E, Isupov MN, Iida Y, Kobayashi T, Izumi Y (2004). Modification of halogen specificity of a vanadium-dependent bromoperoxidase. Protein Sci, 13(6), 1566-1571. Abstract.  Author URL. DOI.
Rand RP, Ho MW, Littlechild JA, Finney JL, Cupane A, Engberts JBFN (2004). Probing the role of water in protein conformation and function. Philosophical Transactions of the Royal Society B: Biological Sciences, 359(1448), 1277-1285. Abstract. DOI.
Littlechild JA, Isupov MN, Line K (2004). The crystal structure of a (-)?-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site. Journal of Molecular Biology, 338(3), 519-532. DOI.
Toogood HS, Brown RC, Line K, Keene PA, Taylor SJC, McCague R, Littlechild JA (2004). The use of a thermostable signature amidase in the resolution of the bicyclic synthon (rac)-γ-lactam. Tetrahedron, 60(3), 711-716. Abstract. DOI.
Halling PJ, Finney JL, Ho MW, Franks F, Littlechild JA (2004). What can we learn by studying enzymes in non-aqueous media?. Philosophical Transactions of the Royal Society B: Biological Sciences, 359(1448), 1287-1297. Abstract. DOI.
Guy JE, Isupov MN, Littlechild JA (2003). Crystallization and preliminary X-ray diffraction studies of a novel alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix. Acta Crystallogr D Biol Crystallogr, 59(Pt 1), 174-176. Abstract.  Author URL.  Full text.
Watts, A. Giles, G.I. Fry, F.H. (2003). Metal and Redox Regulation of Cysteine Protein Function. Chemistry and Biology, 10, 677-693.
Littlechild JA, Garcia-Rodriguez E (2003). Structural studies on the dodecameric vanadium bromoperoxidase from Corallina species. Coordination Chemistry Reviews, 237(1-2), 65-76. DOI.
Littlechild JA, Guy JE, Isupov MN (2003). The Structure of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix. Journal of Molecular Biology, 331(5), 1041-1051. DOI.
Watts AB, Beecher J, Whitcher CS, Littlechild JA (2002). A method for screening Baeyer-Villiger monooxygenase activity against monocyclic ketones. Biocatalysis and Biotransformation, 20(3), 209-214. Abstract. DOI.
Toogood HS, Hollingsworth EJ, Brown RC, Taylor IN, Taylor SJC, McCague R, Littlechild JA (2002). A thermostable L-aminoacylase from Thermococcus litoralis: cloning, overexpression, characterization, and applications in biotransformations. Extremophiles, 6(2), 111-122. Abstract.  Author URL.
Hollingsworth EJ, Isupov MN, Littlechild JA (2002). Crystallization and preliminary X-ray diffraction analysis of L-aminoacylase from the hyperthermophilic archaeon Thermococcus litoralis. Acta Crystallogr D Biol Crystallogr, 58(Pt 3), 507-510. Abstract.  Author URL.  Full text.
Gloyn AL, Noordam K, Willemsen MA, Watts AB, Littlechild JA, Ellard S, Hatterlsey AT (2002). Familial hyperinsulinism caused by a novel activating glucokinase mutation. DIABETES, 51, A260-A260. Author URL.
Toogood HS, Taylor IN, Brown RC, Taylor SJC, McCague R, Littlechild JA (2002). Immobilisation of the thermostable L-aminoacylase from Thermococcus litoralis to generate a reusable industrial biocatalyst. Biocatalysis and Biotransformation, 20(4), 241-249. Abstract. DOI.
Littlechild J, Garcia-Rodriguez E, Dalby A, Isupov M (2002). Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes. J Mol Recognit, 15(5), 291-296. Abstract.  Author URL. DOI.
Qi JF, Isupov MN, Littlechild JA, Anderson LE (2001). Chloroplast glyceraldehyde-3-P dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. BIOCHEMISTRY, 40(29), 8615-8615. Author URL.
Qi JF, Isupov MN, Littlechild JA, Anderson LE (2001). Chloroplast glyceraldehyde-3-P dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 222, U121-U121. Author URL.
Littlechild JA, Anderson LE, Isupov MN, Qi JF (2001). Chloroplast glyceraldehydes-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. Journal of Biological Chemistry, 276(38), 35247-35252. DOI.
Harris JR, Schröder E, Isupov MN, Scheffler D, Kristensen P, Littlechild JA, Vagin AA, Meissner U (2001). Comparison of the decameric structure of peroxiredoxin-II by transmission electron microscopy and X-ray crystallography. Biochim Biophys Acta, 1547(2), 221-234. Abstract.  Author URL.
Gonsalvez IS, Isupov MN, Littlechild JA (2001). Crystallization and preliminary X-ray analysis of a gamma-lactamase. Acta Crystallogr D Biol Crystallogr, 57(Pt 2), 284-286. Abstract.  Author URL.  Full text.
Turner NA, Gaskin DJ, Yagnik AT, Littlechild JA, Vulfson EN (2001). Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones. Protein Eng, 14(4), 269-278. Abstract.  Author URL.
Littlechild JA, Isupov M (2001). Glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus solfataricus. Methods Enzymol, 331, 105-117. Author URL.
Crowhurst G, McHarg J, Littlechild JA (2001). Phosphoglycerate kinases from bacteria and archaea. Methods Enzymol, 331, 90-104. Author URL.
Singleton MR, Littlechild JA (2001). Pyrrolidone carboxylpeptidase from Thermococcus litoralis. Methods Enzymol, 330, 394-403. Author URL.
Littlechild J, Isupov M, Garcia E (2001). Structural studies on the dodecameric vanadium containing bromoperoxidase from Corallina officinalis. JOURNAL OF INORGANIC BIOCHEMISTRY, 86(1), 67-67. Author URL.
Dalby AR, Tolan DR, Littlechild JA (2001). The structure of human liver fructose-1,6-bisphosphate aldolase. Acta Crystallogr D Biol Crystallogr, 57(Pt 11), 1526-1533. Abstract.  Author URL.
Singleton MR, Taylor SJ, Parrat JS, Littlechild JA (2000). Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis. Extremophiles, 4(5), 297-303. Abstract.  Author URL.
Schröder E, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Isupov MN (2000). Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 a resolution. Structure, 8(6), 605-615. Abstract.  Author URL.
Schroder E, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Isupov MN (2000). Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 angstrom resolution (vol 8, pg 605, 2000). STRUCTURE, 8(12), U5-U5. Author URL.
Isupov MN, Dalby AR, Brindley AA, Izumi Y, Tanabe T, Murshudov GN, Littlechild JA (2000). Crystal structure of dodecameric vanadium-dependent bromoperoxidase from the red algae Corallina officinalis. J Mol Biol, 299(4), 1035-1049. Abstract.  Author URL. DOI.
Bourne PC, Isupov MN, Littlechild JA (2000). The atomic-resolution structure of a novel bacterial esterase. Structure, 8(2), 143-151. Abstract.  Author URL.
Charron C, Talfournier F, Isupov MN, Littlechild JA, Branlant G, Vitoux B, Aubry A (2000). The crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus in the presence of NADP(+) at 2.1 a resolution. J Mol Biol, 297(2), 481-500. Abstract.  Author URL. DOI.
Dalby A, Dauter Z, Littlechild JA (1999). Crystal structure of human muscle aldolase complexed with fructose 1,6-bisphosphate: mechanistic implications. Protein Sci, 8(2), 291-297. Abstract.  Author URL. DOI.
Isupov MN, Fleming TM, Dalby AR, Crowhurst GS, Bourne PC, Littlechild JA (1999). Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. J Mol Biol, 291(3), 651-660. Abstract.  Author URL. DOI.
Schröder E, Isupov MN, Naran A, Littlechild JA (1999). Crystallization and preliminary X-ray analysis of human thioredoxin peroxidase-B from red blood cells. Acta Crystallogr D Biol Crystallogr, 55(Pt 2), 536-538. Abstract.  Author URL.  Full text.
Charron C, Talfournier F, Isupov MN, Branlant G, Littlechild JA, Vitoux B, Aubry A (1999). Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus. Acta Crystallogr D Biol Crystallogr, 55(Pt 7), 1353-1355. Abstract.  Author URL.  Full text.
Singleton MR, Isupov MN, Littlechild JA (1999). Crystallization and preliminary X-ray diffraction studies of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Acta Crystallogr D Biol Crystallogr, 55(Pt 3), 702-703. Abstract.  Author URL.  Full text.
Bourne PC, Isupov MN, Littlechild JA (1999). Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase. Acta Crystallogr D Biol Crystallogr, 55(Pt 4), 915-917. Abstract.  Author URL.  Full text.
Littlechild J (1999). Haloperoxidases and their role in biotransformation reactions. Curr Opin Chem Biol, 3(1), 28-34. Abstract.  Author URL.
McHarg J, Kelly SM, Price NC, Cooper A, Littlechild JA (1999). Site-directed mutagenesis of proline 204 in the 'hinge' region of yeast phosphoglycerate kinase. Eur J Biochem, 259(3), 939-945. Abstract.  Author URL.
Crowhurst GS, Dalby AR, Isupov MN, Campbell JW, Littlechild JA (1999). Structure of a phosphoglycerate mutase:3-phosphoglyceric acid complex at 1.7 A. Acta Crystallogr D Biol Crystallogr, 55(Pt 11), 1822-1826. Abstract.  Author URL.  Full text.
Singleton M, Isupov M, Littlechild J (1999). X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Structure, 7(3), 237-244. Abstract.  Author URL.
Fleming TM, Jones CE, Piper PW, Cowan DA, Isupov MN, Littlechild JA (1998). Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. Acta Crystallogr D Biol Crystallogr, 54(Pt 4), 671-674. Abstract.  Author URL.  Full text.
McGhie EJ, Isupov MN, Schröder E, Littlechild JA (1998). Crystallization and preliminary X-ray diffraction studies of the oxygenating subunit of 3,6-diketocamphane monooxygenase from Pseudomonas putida. Acta Crystallogr D Biol Crystallogr, 54(Pt 5), 1035-1038. Abstract.  Author URL.  Full text.
Brindley AA, Dalby AR, Isupov MN, Littlechild JA (1998). Preliminary X-ray analysis of a new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis. Acta Crystallogr D Biol Crystallogr, 54(Pt 3), 454-457. Abstract.  Author URL.  Full text.
Lewis G, Bevan J, Rawas A, McMichael P, Wisdom R, McCague R, Watson H, Littlechild J (1997). Crystallization and preliminary X-ray studies on Candida cylindracea lipase. Acta Crystallogr D Biol Crystallogr, 53(Pt 3), 348-351. Abstract.  Author URL. DOI.
Yagnik AT, Littlechild JA, Turner NJ (1997). Molecular modelling studies of substrate binding to the lipase from Rhizomucor miehei. J Comput Aided Mol Des, 11(3), 256-264. Abstract.  Author URL.
Bentley PA, Kroutil W, Littlechild JA, Roberts SM (1997). Preparation of polyamino acid catalysts for use in Julia asymmetric epoxidation. Chirality, 9(2), 198-202. Abstract.
Fleming T, Littlechild J (1997). Sequence and structural comparison of thermophilic phosphoglycerate kinases with a mesophilic equivalent. Comp Biochem Physiol a Physiol, 118(3), 439-451. Abstract.  Author URL.
Isupov MN, Obmolova G, Butterworth S, BadetDenisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A (1997). Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: Evidence from the 1.8 angstrom crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase (vol 4, pg 801, 1996). STRUCTURE, 5(5), 723-723. Author URL.
Piper PW, Emson C, Jones CE, Cowan DA, Fleming TM, Littlechild JA (1996). Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic Archaeon Sulfolobus solfataricus. Curr Genet, 29(6), 594-596. Abstract.  Author URL.
Dalby A, Rawas A, Watson HC, Littlechild JA (1996). Crystallisation and preliminary x-ray diffraction studies on human liver aldolase. Protein and Peptide Letters, 3(3), 207-212. Abstract.
Brabban AD, Littlechild J, Wisdom R (1996). Stereospecific γ-lactamase activity in a Pseudomonas fluorescens species. Journal of Industrial Microbiology, 16(1), 8-14. Abstract. DOI.
Mcharg J, Littlechild JA (1996). Studies with inhibitors of the glycolytic enzyme phosphoglycerate kinase for potential treatment of cardiovascular and respiratory disorders. J Pharm Pharmacol, 48(2), 201-205. Abstract.  Author URL.
Dalby A, Littlechild JA (1996). Studies with type I aldolase to understand fructose intolerance and combat parasitic disease. J Pharm Pharmacol, 48(2), 214-217. Abstract.  Author URL.
Isupov MN, Obmolova G, Butterworth S, Badet-Denisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A (1996). Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: evidence from the 1.8 a crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase. Structure, 4(7), 801-810. Abstract.  Author URL.
McGhie EJ, Littlechild JA (1996). The purification and crystallisation of 2,5-diketocamphane 1,2-monooxygenase and 3,6-diketocamphane 1,6-monooxygenase from Pseudomonas putida NCIMB 10007. Biochem Soc Trans, 24(1). Author URL.
Fleming TM, Jones CE, Piper PW, Cowan DA, Adams MWW, Littlechild JA (1996). The purification and crystallisation of phosphoglycerate kinase from the hyperthermophilic eubacterium thermotoga maritima. Protein and Peptide Letters, 3(3), 213-218. Abstract.
Jones CE, Fleming TM, Piper PW, Littlechild JA, Cowan DA (1995). Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli. Gene, 160(1), 101-103. Abstract.  Author URL.
Littlechild J, Turner N, Hobbs G, Lilly M, Rawas A, Watson H (1995). Crystallization and preliminary X-ray crystallographic data with Escherichia coli transketolase. Acta Crystallogr D Biol Crystallogr, 51(Pt 6), 1074-1076. Abstract.  Author URL. DOI.
Rush C, Willetts A, Davies G, Dauter Z, Watson H, Littlechild J (1995). Purification, crystallisation and preliminary X-ray analysis of the vanadium-dependent haloperoxidase from Corallina officinalis. FEBS Lett, 359(2-3), 244-246. Abstract.  Author URL.
Jones CE, Fleming TM, Cowan DA, Littlechild JA, Piper PW (1995). The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli. Eur J Biochem, 233(3), 800-808. Abstract.  Author URL.
Davies GJ, Gamblin SJ, Littlechild JA, Dauter Z, Wilson KS, Watson HC (1994). Structure of the ADP complex of the 3-phosphoglycerate kinase from Bacillus stearothermophilus at 1.65 A. Acta Crystallogr D Biol Crystallogr, 50(Pt 2), 202-209. Abstract.  Author URL. DOI.
Littlechild JA, Watson HC (1993). A data-based reaction mechanism for type I fructose bisphosphate aldolase. Trends Biochem Sci, 18(2), 36-39. Abstract.  Author URL.
Taylor SJ, McCague R, Wisdom R, Lee C, Dickson K, Ruecroft G, O'Brien F, Littlechild J, Bevan J, Roberts SM, et al (1993). Development of the biocatalytic resolution of 2-azabicyclo[2.2.1]hept-5-en-3-one as an entry to single-enantiomer carbocyclic nucleosides. Tetrahedron: Asymmetry, 4(6), 1117-1128. Abstract. DOI.
BARBER MD, GAMBLIN SJ, WATSON HC, LITTLECHILD JA (1993). SITE-DIRECTED MUTAGENESIS OF YEAST PHOSPHOGLYCERATE KINASE - ARGININE-65, ARGININE-121 AND ARGININE-168. FEBS LETTERS, 320(3), 193-197. Author URL. DOI.
Vellieux FM, Hajdu J, Verlinde CL, Groendijk H, Read RJ, Greenhough TJ, Campbell JW, Kalk KH, Littlechild JA, Watson HC, et al (1993). Structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei determined from Laue data. Proc Natl Acad Sci U S A, 90(6), 2355-2359. Abstract.  Author URL.
Davies GJ, Gamblin SJ, Littlechild JA, Watson HC (1993). The structure of a thermally stable 3-phosphoglycerate kinase and a comparison with its mesophilic equivalent. Proteins, 15(3), 283-289. Abstract.  Author URL. DOI.
Joao HC, Williams RJ, Littlechild JA, Nagasuma R, Watson HC (1992). An investigation of large inhibitors binding to phosphoglycerate kinase and their effect on anion activation. Eur J Biochem, 205(3), 1077-1088. Abstract.  Author URL.
Walker PA, Joâo HC, Littlechild JA, Williams RJ, Watson HC (1992). Characterisation of yeast phosphoglycerate kinase modified by mutagenesis at residue 21. Eur J Biochem, 207(1), 29-37. Abstract.  Author URL.
Eisenberg D, Perutz MF, Buckingham AD, Graf L, Thornton J, Blow DM, Fersht AR, Karplus M, Johnson LN, Rippmann F, et al (1992). General discussion. Faraday Discussions, 93, 107-129. DOI.
Davies GJ, Gamblin SJ, Littlechild JA, Watson HC (1992). Purification, crystallization and preliminary X-ray analysis of the 3-phosphoglycerate kinase from Bacillus stearothermophilus. J Mol Biol, 227(4), 1263-1264. Abstract.  Author URL.
Graham HC, Williams RJ, Littlechild JA, Watson HC (1991). A proton-NMR study of a site-directed mutation (His388----Glu) in the interdomain region of yeast phosphoglycerate kinase. Implications for domain movement. Eur J Biochem, 196(2), 261-269. Abstract.  Author URL.
Gamblin SJ, Davies GJ, Grimes JM, Jackson RM, Littlechild JA, Watson HC (1991). Activity and specificity of human aldolases. J Mol Biol, 219(4), 573-576. Abstract.  Author URL.
Littlechild JA (1991). Protein crystallization: Magical or logical: can we establish some general rules?. Journal of Physics D: Applied Physics, 24(2), 111-118. Abstract. DOI.
Davies GJ, Littlechild JA, Watson HC, Hall L (1991). Sequence and expression of the gene encoding 3-phosphoglycerate kinase from Bacillus stearothermophilus. Gene, 109(1), 39-45. Abstract.  Author URL.
Minard P, Bowen DJ, Hall L, Littlechild JA, Watson HC (1990). Site-directed mutagenesis of aspartic acid 372 at the ATP binding site of yeast phosphoglycerate kinase: over-expression and characterization of the mutant enzyme. Protein Eng, 3(6), 515-521. Abstract.  Author URL.
Gamblin SJ, Cooper B, Millar JR, Davies GJ, Littlechild JA, Watson HC (1990). The crystal structure of human muscle aldolase at 3.0 a resolution. FEBS Lett, 262(2), 282-286. Abstract.  Author URL.
Fairbrother WJ, Walker PA, Minard P, Littlechild JA, Watson HC, Williams RJ (1989). NMR analysis of site-specific mutants of yeast phosphoglycerate kinase. An investigation of the triose-binding site. Eur J Biochem, 183(1), 57-67. Abstract.  Author URL.
Fairbrother WJ, Hall L, Littlechild JA, Walker PA, Watson HC, Williams RJ (1989). Site-directed mutagenesis of histidine 62 in the 'basic patch' region of yeast phosphoglycerate kinase. FEBS Lett, 258(2), 247-250. Abstract.  Author URL.
Walker PA, Littlechild JA, Hall L, Watson HC (1989). Site-directed mutagenesis of yeast phosphoglycerate kinase. The 'basic-patch' residue arginine 168. Eur J Biochem, 183(1), 49-55. Abstract.  Author URL.
Wilson HR, Williams RJ, Littlechild JA, Watson HC (1988). NMR analysis of the interdomain region of yeast phosphoglycerate kinase. Eur J Biochem, 170(3), 529-538. Abstract.  Author URL.
Bowen D, Littlechild JA, Fothergill JE, Watson HC, Hall L (1988). Nucleotide sequence of the phosphoglycerate kinase gene from the extreme thermophile Thermus thermophilus. Comparison of the deduced amino acid sequence with that of the mesophilic yeast phosphoglycerate kinase. Biochem J, 254(2), 509-517. Abstract.  Author URL.
FAIRBROTHER WJ, HALL L, LITTLECHILD JA, WALKER PA, WATSON HC, WILLIAMS RJP (1988). PROBING THE 3-PHOSPHOGLYCERATE-BINDING SITE OF YEAST PHOSPHOGLYCERATE KINASE USING SITE-SPECIFIC MUTANTS AND H-1 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY. BIOCHEMICAL SOCIETY TRANSACTIONS, 16(5), 724-725. Author URL.
FAIRBROTHER WJ, HALL L, LITTLECHILD J, MINARD P, WATSON HC, WILLIAMS RJP (1987). ANION BINDING STUDY OF YEAST PHOSPHOGLYCERATE KINASE BY NMR AND SITE-SPECIFIC MUTAGENESIS. PROTEIN ENGINEERING, 1(3), 261-261. Author URL.
FAIRBROTHER WJ, HALL L, LITTLECHILD JA, MINARD P, WATSON HC, WILLIAMS RJP (1987). ANION BINDING STUDY OF YEAST PHOSPHOGLYCERATE KINASE BY NUCLEAR-MAGNETIC-RESONANCE AND SITE-SPECIFIC MUTAGENESIS. BIOCHEMICAL SOCIETY TRANSACTIONS, 15(5), 868-869. Author URL.
BOWEN D, DAVIES G, GAMBLIN S, HALL L, LITTLECHILD J, WATSON HC (1987). PHOSPHOGLYCERATE KINASE - a TEST-BED SYSTEM FOR THE STUDY OF PROTEIN THERMOSTABILITY. PROTEIN ENGINEERING, 1(3), 242-242. Author URL.
Littlechild JA, Davies GJ, Gamblin SJ, Watson HC (1987). Phosphoglycerate kinase from the extreme thermophile Thermus thermophilus Crystallization and preliminary X-ray data. FEBS Letters, 225(1-2), 123-126. Abstract. DOI.
MINARD P, WALKER P, BOWEN D, DAVIES G, LITTLECHILD J, HALL L, WATSON HC (1987). THE USE OF SITE-DIRECTED MUTAGENESIS TO STUDY THE GLYCOLYTIC ENZYME PHOSPHOGLYCERATE KINASE. PROTEIN ENGINEERING, 1(3), 260-260. Author URL.
Littlechild J, Malcolm A, Paterakis K, Ackermann I, Dijk J (1987). The tertiary structure of salt-extracted ribosomal proteins from Escherichia coli as studied by proton magnetic resonance spectroscopy and limited proteolysis experiments. Biochim Biophys Acta, 913(2), 245-255. Abstract.  Author URL.
Crout DHG, Littlechild J, Morrey SM (1986). Acetoin metabolism: Stereochemistry of the acetoin produced by the pyruvate decarboxylase of wheat germ and by the α-acetolactate decarboxylase of Klebsiella aerogenes. Journal of the Chemical Society, Perkin Transactions 1, 105-108. Abstract.
LITTLECHILD JA (1986). THE TYPE-II RESTRICTION ENZYMES HGIAI AND TAQI - PURIFICATION AND PROPERTIES. BIOCHEMICAL SOCIETY TRANSACTIONS, 14(2), 268-268. Author URL.
Dijk J, Littlechild JA, Freund AM, Pouyet J, Daune M, Provencher SW (1986). The secondary structure of salt-extracted ribosomal proteins from Escherichia coli as studied by circular dichroic spectroscopy. Biochim Biophys Acta, 874(2), 227-234. Abstract.  Author URL.
Stöffler-Meilicke M, Epe B, Woolley P, Lotti M, Littlechild J, Stöffler G (1984). Location of protein S4 on the small ribosomal subunit of E. coli and B. stearothermophilus with protein- and hapten-specific antibodies. Mol Gen Genet, 197(1), 8-18. Abstract.  Author URL.
Crout DHG, Littlechild J, Mitchell MB, Morrey SM (1984). Stereochemistry of the decarboxylation of α-acetolactate (2-hydroxy-2-methyl-3-oxobutanoate) by the acetolactate decarboxylase of Klebsiella aerogenes. Journal of the Chemical Society, Perkin Transactions 1, 2271-2276. Abstract.
Paterakis K, Littlechild J, Woolley P (1983). Structural and functional studies on protein S20 from the 30-S subunit of the Escherichia coli ribosome. Eur J Biochem, 129(3), 543-548. Abstract.  Author URL.
Epe B, Woolley P, Steinhäuser KG, Littlechild J (1982). Distance measurement by energy transfer: the 3' end of 16-S RNA and proteins S4 and S17 of the ribosome of Escherichia coli. Eur J Biochem, 129(1), 211-219. Abstract.  Author URL.
Wirth R, Littlechild J, Böck A (1982). Ribosomal protein S20 purified under mild conditions almost completely inhibits its own translation. Mol Gen Genet, 188(1), 164-166. Abstract.  Author URL.
Paterakis K, Littlechild J (1982). Structural domains of ribosomal protein S8 and their relationship to ribosomal RNA binding. FEBS Lett, 149(2), 328-333. Abstract.  Author URL.
Georgalis Y, Giri L, Littlechild JA (1981). Physical studies on the ribosomal protein S2 from the Escherichia coli 30S. Biochemistry, 20(5), 1061-1064. Abstract.  Author URL.
Pawlik RT, Littlechild J, Pon C, Gualerzi C (1981). Purification and properties of Escherichia coli translational initiation factors. Biochemistry International, 2(4), 421-428.
LITTLECHILD JA, MALCOLM AL, SOWDEN BK (1980). COMPARATIVE STUDIES ON THE 30S RIBOSOMAL-PROTEINS FROM ESCHERICHIA-COLI AND BACILLUS-STEAROTHERMOPHILUS. EUROPEAN JOURNAL OF CELL BIOLOGY, 22(1), 133-133. Author URL.
Littlechild J (1980). Proton magnetic resonance studies of Escherichia coli ribosomal protein S16. FEBS Lett, 111(1), 51-55. Author URL.
LITTLECHILD JA, PATERAKIS K (1980). STRUCTURAL STABILITY OF SEVERAL PROTEINS ISOLATED FROM THE 30S-RIBOSOMAL SUBUNIT OF ESCHERICHIA-COLI. EUROPEAN JOURNAL OF CELL BIOLOGY, 22(1), 132-132. Author URL.
Littlechild J, Morrison CA, Bradbury EM (1979). Proton magnetic resonance studies of Escherichia coli ribosomal protein S4 and a C-terminal fragment of this protein. FEBS Lett, 104(1), 90-94. Author URL.
Dijk J, Littlechild J (1979). Purification of ribosomal proteins from Escherichia coli under nondenaturing conditions. Methods Enzymol, 59, 481-502. Author URL.
Khechinashvili NN, Koteliansky VE, Gogia ZV, Littlechild J, Dijk J (1978). A heat denaturation study of several ribosomal proteins from Escherichia coli by scanning microcalorimetry. FEBS Letters, 95(2), 270-272. DOI.
Khechinashvili NN, Koteliansky VE, Gogia ZV, Littlechild J, Dijk J (1978). A heat denaturation study of several ribosomal proteins from Escherichia coli by scanning microcalorimetry. FEBS Lett, 95(2), 270-272. Author URL.
Littlechild JA, Malcolm AL (1978). A new method for the purification of 30S ribosomal proteins from Escherichia coli using nondenaturing conditions. Biochemistry, 17(16), 3363-3369. Abstract.  Author URL.
OSTERBERG R, SJOBERG B, LILJAS A, GARRETT RA, LITTLECHILD J (1978). SMALL-ANGLE X-RAY-SCATTERING STUDY OF COMPLEXES OF INDIVIDUAL COMPONENTS FROM ESCHERICHIA-COLI RIBOSOMES. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 11(OCT), 487-487. Author URL. DOI.
Österberg R, Sjöberg B, Littlechild J (1978). Small-angle X-ray scattering study of the proteins S1, S8, S15, S16, S20 from Escherichia coli ribosomes. FEBS Letters, 93(1), 115-119. DOI.
Osterberg R, Sjöberg B, Garrett RA, Littlechild J (1978). Small-angle X-ray titration of the complex formed between the ribosomal protein S4 and its 16S binding site, S4-RNA: a central core in the 30S subunit. Nucleic Acids Res, 5(10), 3579-3587. Abstract.  Author URL.
Giri L, Littlechild J, Dijk J (1977). Hydrodynamic studies on the Escherichia coli robosomal proteins S8 and L6, prepared by two different methods. FEBS Lett, 79(2), 238-244. Author URL.
Morrison CA, Bradbury EM, Littlechild J, Dijk J (1977). Proton magnetic resonance studies to compare Escherichia coli ribosomal proteins prepared by two different methods. FEBS Lett, 83(2), 348-352. Author URL.
Österberg R, Sjöberg B, Garrett RA, Littlechild J (1977). Small-angle X-ray scattering study of the 16 S RNA binding protein S4 from Escherichia coli ribosomes. FEBS Letters, 73(1), 22-24. DOI.
Osterberg R, Sjöberg B, Garrett RA, Littlechild J (1977). Small-angle x-ray scattering study of the 16S RNA binding protein S4 from Escherichia coli ribosomes. FEBS Lett, 73(1), 25-28. Author URL.
Dijk J, Littlechild J, Garrett RA (1977). The RNA binding properties of "native" protein-protein complexes isolated from the Escherichia coli ribosome. FEBS Lett, 77(2), 295-300. Author URL.
Littlechild J, Dijk J, Garrett RA (1977). The identification of new RNA-binding proteins in the Escherichia coli ribosome. FEBS Lett, 74(2), 292-294. Author URL.
Littlechild J (1973). Nuclease contaminant of a batch of lysozyme used for ribosome extraction: cleavage of Escherichia coli 23S rRNA into two specific fragments. Anal Biochem, 55(2), 634-636. Author URL.
Littlechild J, Spencer M (1973). Stability and homogeneity of preparations of ribosomal particles from Escherichia coli. Biochemistry, 12(16), 3102-3108. Author URL.

Chapters

Littlechild J, Isupov M (2014). Haloperoxidase enzymes as 'redox catalysts' important for industrial biocatalysis. In  (Ed) Recent Advances in Redox Active Plant and Microbial Products: from Basic Chemistry to Widespread Applications in Medicine and Agriculture, 425-446.  Abstract. DOI.
James PBC, sayer C, Novak H, Littlechild JA (2013). Mechanisms of Thermal Stability Adopted by Thermophilic Proteins and Their Use in White Biotechnology. In Littlechild JA, Satyanarayana T, Kawarabayasi Y (Eds.) Thermophilic Microbes in Environmental and Industrial Biotechnology, Springer Netherlands, 481-507. DOI.
Littlechild JA, Hill A (2012). Oxidation:Haloperoxidases. In Carreira EM, Yamamoto H (Eds.) Comprehensive Chirality, Amsterdam: Elsevier, 329-349.  Abstract.
Szabo K, Gutowski NJ, Holley JE, Littlechild JA, Winyard P (2009). Redox Control in Human Disease with a Special Emphasis on the Peroxiredoxin-Based Antioxidant System. In Jacob C, Winyard PG (Eds.) Redox signaling and regulation in biology and medicine, Vch Verlagsgesellschaft Mbh, 409-431.  Abstract.
Szabo KE, Line K, Eggleton P, Littlechild JA, Winyard P (2009). Structure and Function of the Human Peroxiredoxin-Based Antioxidant System:the Interplay between Peroxiredoxins,Thioredoxins,Thioredoxin Reductases,Sulfiredoxins and Sestrins. In Jacob C, Winyard PG (Eds.) Redox signaling and regulation in biology and medicine, Vch Verlagsgesellschaft Mbh, 143-174.  Abstract.
Szabo KE, Line K, Eggleton P, Littlechild JA, Winyard PG (2009). Structure and function of the human peroxiredoxin-based antioxidant system: the interplay between peroxierdoxins, thioredoxins, thioredoxin reductases, sulfiredoxins and sestrins. In Jacob C, Winyard PG (Eds.) Redox Signaling and Regulation in Biology and Medicine, Weinheim, Germany: Wiley-VCH.
Littlechild JA, Garcia-Rodriguez E, Coupe E, Watts A, Isupov M (2007). Structural Studies of Vanadium Haloperoxidases:Insight into Halide Specificity,Stability and Enzyme Mechanism. In Kustin K, Pessoa JC, Crans D (Eds.) Vanadium:The versatile Metal, Washington, USA: ACS, 136-147.
Littlechild J, Garcia-Rodriguez E, Watts A, Coupe E, Isupov M (2007). The vanadium dependent bromoperoxidase enzymes:Insights into Mechanism and Applications gained from Structural Studies. In Aureliano M (Ed) Vanadium biochemistry, India: Signpost, 97-116.
Littlechild JA, Garcia-Rodriguez E, Watts A, Coupe E, Isupov M (2007). The vanadium dependent bromoperoxidase enzymes:Insights into Mechanism and Applications gained from Structural Studies. In Alves MA (Ed) Vanadium Biochemistry, India: Research Signpost, 97-116.

Conferences

Szabo-Taylor KE, Lo Faro ML, Eggleton P, Turner CAL, Tarr JM, Haigh RC, Littlechild JA, Whiteman M, Winyard PG (2010). Peroxiredoxin 2 in Human Inflammatory Joint Disease.  Author URL. DOI.
Turner CA, Szabo KE, Haigh R, Tarr J, Littlechild JA, Eggleton P, Winyard PG (2009). EXPRESSION OF THE PEROXIREDOXIN-BASED ANTIOXIDANT SYSTEM IN PERIPHERAL BLOOD LYMPHOCYTES IN RHEUMATOID ARTHRITIS.  Author URL.
Gutowski NJ, Watson EL, Holley JE, Winyard PG, Littlechild JA (2009). Peroxiredoxin II: an antioxidant with therapeutic potential in multiple sclerosis?.  Author URL.
Szabo K, Tarr J, Eggleton P, Line K, Ryan B, Aksu K, Akcay Y, Haigh R, Littlechild J, Winyard P, et al (2008). Extracellular peroxiredoxin II in rheumatoid arthritis and other autoimmune conditions.  Author URL.
Taylor IN, Brown RC, Bycroft M, King G, Littlechild JA, Lloyd MC, Praquin C, Toogood HS, Taylor SJC (2004). Application of thermophilic enzymes in commercial biotransformation processes. England.  Abstract.  Author URL.
Littlechild JA, Guy JE, Isupov MN (2004). Hyperthermophilic dehydrogenase enzymes. England.  Abstract.  Author URL.
Whitcher CS, Littlechild JA, Woodley JM (2003). Application of a Baeyer-Villiger monooxygenase from cunninghamella echinulata NRRL 3655.  Author URL.
Crowhurst GS, Isupov MN, Fleming T, Littlechild JA (1998). Two glycolytic enzymes from Sulfolobus solfataricus. England.  Author URL.
Lilly MD, Chauhan R, French C, Gyamerah M, Hobbs GR, Humphrey A, Isupov M, Littlechild JA, Mitra RK, Morris KG, et al (1996). Carbon-carbon bond synthesis: the impact of rDNA technology on the production and use of E. coli transketolase.  Abstract. DOI.
McGhie EJ, Littlechild JA (1996). The purification and crystallisation of 2,5-diketocamphane 1,2 monooxygenase and 3,6-diketocamphane 1,6 monooxygenase from Pseudomonas putida NCIMB 10007.
Watson HC, Littlechild JA (1990). Isoenzymes of phosphoglycerate kinase: evolutionary conservation of the structure of this glycolytic enzyme. England.  Author URL.

Publications by year



In Press

Littlechild JA (In Press). Improving the ‘Tool Box’ for Robust Industrial Enzymes. Journal of Industrial Microbiology & Biotechnology Full text.

2017

Antranikian G, Suleiman M, Schäfers C, Adams MWW, Bartolucci S, Blamey JM, Birkeland N-K, Bonch-Osmolovskaya E, da Costa MS, Cowan D, et al (2017). Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island. Extremophiles, 21(4), 733-742. Abstract.  Author URL. DOI.
de Rose SA, Novak H, Dowd A, Singh S, Lang DA, Littlechild J (2017). Stabilization of a lipolytic enzyme for commercial application. Catalysts, 7(3). Abstract. DOI.

2016

Finnigan W, Thomas A, Cromar H, Gough B, Snajdrova R, Adams JP, Littlechild JA, Harmer NJ (2016). Characterization of carboxylic acid reductases as enzymes in the toolbox for synthetic chemistry. ChemCatChem, in press Full text. DOI.
Zarafeta D, Kissas D, Sayer C, Gudbergsdottir SR, Ladoukakis E, Isupov MN, Chatziioannou A, Peng X, Littlechild JA, Skretas G, et al (2016). Discovery and Characterization of a Thermostable and Highly Halotolerant GH5 Cellulase from an Icelandic Hot Spring Isolate. PLoS One, 11(1). Abstract.  Author URL.  Full text. DOI.
sayer C, Finnigan W, Isupov MN, Levisson M, Kengen SWM, van der Oost J, Harmer NJ, Littlechild JA (2016). Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site. Scientific Reports, 6, 25542-25542. Abstract.  Full text. DOI.

2015

Littlechild JA (2015). Archaeal Enzymes and Applications in Industrial Biocatalysts. Archaea, 2015, 1-10. Full text. DOI.
Ferrandi EE, Sayer C, Isupov MN, Annovazzi C, Marchesi C, Iacobone G, Peng X, Bonch-Osmolovskaya E, Wohlgemuth R, Littlechild JA, et al (2015). Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries. FEBS J, 282(15), 2879-2894. Abstract.  Author URL. DOI.
Littlechild JA (2015). Enzymes from Extreme Environments and Their Industrial Applications. Front Bioeng Biotechnol, 3 Abstract.  Author URL.  Full text. DOI.
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from a new Planctomycetes species, Thermogutta terrifontis. FEBS J, 282(15), 2846-2857. Abstract.  Author URL. DOI.
Sayer C, Szabo Z, Isupov MN, Ingham C, Littlechild JA (2015). The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain. Front Microbiol, 6 Abstract.  Author URL.  Full text. DOI.
Isupov MN, Schröder E, Gibson RP, Beecher J, Donadio G, Saneei V, Dcunha SA, McGhie EJ, Sayer C, Davenport CF, et al (2015). The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of a type II Baeyer-Villiger monooxygenase. Acta Crystallogr D Biol Crystallogr, 71(Pt 11), 2344-2353. Abstract.  Author URL.  Full text. DOI.

2014

Novak HR, Sayer C, Isupov MN, Gotz D, Spragg AM, Littlechild JA (2014). Biochemical and structural characterisation of a haloalkane dehalogenase from a marine Rhodobacteraceae. FEBS Lett, 588(9), 1616-1622. Abstract.  Author URL.  Full text. DOI.
Kallnik V, Bunescu A, Sayer C, Bräsen C, Wohlgemuth R, Littlechild J, Siebers B (2014). Characterization of a phosphotriesterase-like lactonase from the hyperthermoacidophilic crenarchaeon Vulcanisaeta moutnovskia. J Biotechnol, 190, 11-17. Abstract.  Author URL. DOI.
Vivoli M, Novak HR, Littlechild JA, Harmer NJ (2014). Determination of protein-ligand interactions using differential scanning fluorimetry. J Vis Exp(91). Abstract.  Author URL.  Full text. DOI.
Littlechild J, Isupov M (2014). Haloperoxidase enzymes as 'redox catalysts' important for industrial biocatalysis. In  (Ed) Recent Advances in Redox Active Plant and Microbial Products: from Basic Chemistry to Widespread Applications in Medicine and Agriculture, 425-446.  Abstract. DOI.
Littlechild J, Sayer C, Isupov M, Ward J, Littlechild J (2014). Structural studies on transaminase enzymes and applications in biocatalysis. NEW BIOTECHNOLOGY, 31, S19-S19. Author URL. DOI.
James P, Isupov MN, Sayer C, Saneei V, Berg S, Lioliou M, Kotlar HK, Littlechild JA (2014). The structure of a tetrameric α-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability. Acta Crystallogr D Biol Crystallogr, 70(Pt 10), 2607-2618. Abstract.  Author URL.  Full text. DOI.
Sayer C, Martinez-Torres RJ, Richter N, Isupov MN, Hailes HC, Littlechild JA, Ward JM (2014). The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca. FEBS J, 281(9), 2240-2253. Abstract.  Author URL.  Full text. DOI.

2013

Holley JE, Slonka J, Littlechild J, Gutowski NJ (2013). An investigation into the presence of pyroglutamyl peptidases in the Alzheimer's disease brain: relevance to pathology?. JOURNAL OF NEUROLOGY, 260, S167-S167. Author URL.
Novak HR, Sayer C, Panning J, Littlechild JA (2013). Characterisation of an L-haloacid dehalogenase from the marine psychrophile Psychromonas ingrahamii with potential industrial application. Mar Biotechnol (NY), 15(6), 695-705. Abstract.  Author URL. DOI.
Novak HR, Sayer C, Panning J, Littlechild JA (2013). Characterisation of an l-Haloacid Dehalogenase from the Marine Psychrophile Psychromonas ingrahamii with Potential Industrial Application. Marine Biotechnology, 15(6), 695-705. Abstract. DOI.
Reid EL, Weynberg KD, Love J, Isupov MN, Littlechild JA, Wilson WH, Kelly SL, Lamb DC, Allen MJ (2013). Functional and structural characterisation of a viral cytochrome b5. FEBS Lett, 587(22), 3633-3639. Abstract.  Author URL. DOI.
Novak HR, Sayer C, Isupov MN, Paszkiewicz K, Gotz D, Spragg AM, Littlechild JA (2013). Marine Rhodobacteraceae L-haloacid dehalogenase contains a novel His/Glu dyad that could activate the catalytic water. FEBS J, 280(7), 1664-1680. Abstract.  Author URL.  Full text. DOI.
Novak H, Littlechild J (2013). Marine enzymes with applications for biosynthesis of fine chemicals. , 89-106. Abstract. DOI.
James PBC, sayer C, Novak H, Littlechild JA (2013). Mechanisms of Thermal Stability Adopted by Thermophilic Proteins and Their Use in White Biotechnology. In Littlechild JA, Satyanarayana T, Kawarabayasi Y (Eds.) Thermophilic Microbes in Environmental and Industrial Biotechnology, Springer Netherlands, 481-507. DOI.
Satyanarayana T, Littlechild J, Kawarabayasi Y (2013). Preface. DOI.
Littlechild JA (2013). Protein structure and function. , 57-79. Abstract. DOI.
Sayer C, Isupov MN, Westlake A, Littlechild JA (2013). Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity. Acta Crystallogr D Biol Crystallogr, 69(Pt 4), 564-576. Abstract.  Author URL.  Full text. DOI.

2012

Hill A, Littlechild J (2012). 7.15 Oxidation: Haloperoxidases. , 7, 329-349. Abstract. DOI.
Fraser CS, Rubio-Cabezas O, Littlechild JA, Ellard S, Hattersley AT, Flanagan SE (2012). Amino acid properties may be useful in predicting clinical outcome in patients with Kir6.2 neonatal diabetes. Eur J Endocrinol, 167(3), 417-421. Abstract.  Author URL. DOI.
Sayer C, Bommer M, Isupov M, Ward J, Littlechild J (2012). Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus. Acta Crystallographica Section D: Biological Crystallography, 68(7), 763-772. Abstract.  Full text. DOI.
Littlechild JA, Greenway G, Watts P, Hickey AM, Ngamsom B (2012). Immobilised Enzymes and Co-Factors.
Szabo-Taylor KE, Eggleton P, Turner CAL, Lo Faro ML, Tarr JM, Toth S, Whiteman M, Haigh RC, Littlechild JA, Winyard PG, et al (2012). Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 44(8), 1223-1231. Author URL. DOI.
Littlechild JA, Hill A (2012). Oxidation:Haloperoxidases. In Carreira EM, Yamamoto H (Eds.) Comprehensive Chirality, Amsterdam: Elsevier, 329-349.  Abstract.

2011

Reid EL, Worthy CA, Probert I, Ali ST, Love J, Napier J, Littlechild JA, Somerfield PJ, Allen MJ (2011). Coccolithophores: functional biodiversity, enzymes and bioprospecting. Mar Drugs, 9(4), 586-602. Abstract.  Author URL.  Full text. DOI.
Littlechild JA (2011). Thermophilic archaeal enzymes and applications in biocatalysis. Biochem Soc Trans, 39(1), 155-158. Abstract.  Author URL. DOI.

2010

Wilson RA, Gibson RP, Quispe CF, Littlechild JA, Talbot NJ (2010). An NADPH-dependent genetic switch regulates plant infection by the rice blast fungus. Proc Natl Acad Sci U S A, 107(50), 21902-21907. Abstract.  Author URL. DOI.
Opperman DJ, Sewell BT, Litthauer D, Isupov MN, Littlechild JA, van Heerden E (2010). Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01. Biochem Biophys Res Commun, 393(3), 426-431. Abstract.  Author URL. DOI.
Ngamsom B, Hickey AM, Greenway GM, Littlechild JA, Watts P, Wiles C (2010). Development of a high throughput screening tool for biotransformations utilising a thermophilic l-aminoacylase enzyme. Journal of Molecular Catalysis B: Enzymatic, 63(1-2), 81-86. Abstract. DOI.
Szabo-Taylor KE, Lo Faro ML, Eggleton P, Turner CAL, Tarr JM, Haigh RC, Littlechild JA, Whiteman M, Winyard PG (2010). Peroxiredoxin 2 in Human Inflammatory Joint Disease.  Author URL. DOI.
Ngamsom B, Hickey AM, Greenway GM, Littlechild JA, McCreedy T, Watts P, Wiles C (2010). The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow. Org Biomol Chem, 8(10), 2419-2424. Abstract.  Author URL. DOI.
Willies S, Isupov M, Littlechild J (2010). Thermophilic enzymes and their applications in biocatalysis: a robust aldo-keto reductase. Environ Technol, 31(10), 1159-1167. Abstract.  Author URL. DOI.

2009

Hickey AM, Ngamsom B, Wiles C, Greenway GM, Watts P, Littlechild JA (2009). A microreactor for the study of biotransformations by a cross-linked gamma-lactamase enzyme. Biotechnol J, 4(4), 510-516. Abstract.  Author URL. DOI.
Rye CA, Isupov MN, Lebedev AA, Littlechild JA (2009). Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii. Extremophiles, 13(1), 179-190. Abstract.  Author URL.  Full text. DOI.
Turner CA, Szabo KE, Haigh R, Tarr J, Littlechild JA, Eggleton P, Winyard PG (2009). EXPRESSION OF THE PEROXIREDOXIN-BASED ANTIOXIDANT SYSTEM IN PERIPHERAL BLOOD LYMPHOCYTES IN RHEUMATOID ARTHRITIS.  Author URL.
Gutowski NJ, Watson EL, Holley JE, Winyard PG, Littlechild JA (2009). Peroxiredoxin II: an antioxidant with therapeutic potential in multiple sclerosis?.  Author URL.
Szabo K, Gutowski NJ, Holley JE, Littlechild JA, Winyard P (2009). Redox Control in Human Disease with a Special Emphasis on the Peroxiredoxin-Based Antioxidant System. In Jacob C, Winyard PG (Eds.) Redox signaling and regulation in biology and medicine, Vch Verlagsgesellschaft Mbh, 409-431.  Abstract.
Szabo KE, Line K, Eggleton P, Littlechild JA, Winyard P (2009). Structure and Function of the Human Peroxiredoxin-Based Antioxidant System:the Interplay between Peroxiredoxins,Thioredoxins,Thioredoxin Reductases,Sulfiredoxins and Sestrins. In Jacob C, Winyard PG (Eds.) Redox signaling and regulation in biology and medicine, Vch Verlagsgesellschaft Mbh, 143-174.  Abstract.
Szabo KE, Line K, Eggleton P, Littlechild JA, Winyard PG (2009). Structure and function of the human peroxiredoxin-based antioxidant system: the interplay between peroxierdoxins, thioredoxins, thioredoxin reductases, sulfiredoxins and sestrins. In Jacob C, Winyard PG (Eds.) Redox Signaling and Regulation in Biology and Medicine, Weinheim, Germany: Wiley-VCH.
Willies SC, Isupov MN, Garman EF, Littlechild JA (2009). The binding of haem and zinc in the 1.9 a X-ray structure of Escherichia coli bacterioferritin. J Biol Inorg Chem, 14(2), 201-207. Abstract.  Author URL.  Full text. DOI.
Littlechild J (2009). Thermophilic enzymes as stable biocatalysts. NEW BIOTECHNOLOGY, 25, S108-S108. Author URL. DOI.
Littlechild J, Garcia Rodriguez E, Isupov M (2009). Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography. J Inorg Biochem, 103(4), 617-621. Abstract.  Author URL.  Full text. DOI.

2008

Littlechild J (2008). Catalyst for improvement. EBR - European Biopharmaceutical Review(SUMM), 27-32. Abstract.
Ward S, Macgowan A, Roberts S, Littlechild JA, Line K, Irving E, Donnelly S (2008). Derivatives of 18Beta-Glycyrrhetinic Acid.
Szabo K, Tarr J, Eggleton P, Line K, Ryan B, Aksu K, Akcay Y, Haigh R, Littlechild J, Winyard P, et al (2008). Extracellular peroxiredoxin II in rheumatoid arthritis and other autoimmune conditions.  Author URL.
Ngamsom B, Marle L, Hickey AM, Greenway GM, Watts P, McCreedy T, Littlechild JA (2008). Immobilisation of thermophilic enzymes within miniaturised flow reactors for biotransformations. AIChE Annual Meeting, Conference Proceedings
Line K, Isupov MN, Garcia-Rodriguez E, Maggioli G, Parra F, Littlechild JA (2008). The Fasciola hepatica thioredoxin: High resolution structure reveals two oxidation states. Mol Biochem Parasitol, 161(1), 44-48. Abstract.  Author URL.  Full text. DOI.

2007

Rye CA, Isupov MN, Lebedev AA, Littlechild JA (2007). An order-disorder twin crystal of L-2-haloacid dehalogenase from Sulfolobus tokodaii. Acta Crystallogr D Biol Crystallogr, 63(Pt 8), 926-930. Abstract.  Author URL.  Full text. DOI.
Battershill C, Bavington C, Chahal S, Jaspars M, Littlechild J, Spragg AM (2007). Contributions of marine bioscience to industrial biotechology. Industrial Biotechnology, 3(4), 304-313. Abstract. DOI.
Sayer C, Isupov MN, Littlechild JA (2007). Crystallization and preliminary X-ray diffraction analysis of omega-amino acid:pyruvate transaminase from Chromobacterium violaceum. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63(Pt 2), 117-119. Abstract.  Author URL.  Full text. DOI.
Hickey AM, Marle L, McCreedy T, Watts P, Greenway GM, Littlechild JA (2007). Immobilization of thermophilic enzymes in miniaturized flow reactors. Biochem Soc Trans, 35(Pt 6), 1621-1623. Abstract.  Author URL. DOI.
Littlechild JA, Guy J, Connelly S, Mallett L, Waddell S, Rye CA, Line K, Isupov M (2007). Natural methods of protein stabilization: thermostable biocatalysts. Biochem Soc Trans, 35(Pt 6), 1558-1563. Abstract.  Author URL. DOI.
Littlechild J (2007). Nature's catalysts for biosynthesis of natural products. AMINO ACIDS, 33(3), XIV-XIV. Author URL.
Watts P, McCreedy T, Marle L, Littlechild J, Hickey AM, Greenway GM (2007). ORGN 104-The use of immobilized thermophilic enzymes for organic synthesis in flow reactors. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 234 Author URL.
Littlechild JA, Garcia-Rodriguez E, Coupe E, Watts A, Isupov M (2007). Structural Studies of Vanadium Haloperoxidases:Insight into Halide Specificity,Stability and Enzyme Mechanism. In Kustin K, Pessoa JC, Crans D (Eds.) Vanadium:The versatile Metal, Washington, USA: ACS, 136-147.
Coupe EE, Smyth MG, Fosberry AP, Hall RM, Littlechild JA (2007). The dodecameric vanadium-dependent haloperoxidase from the marine algae Corallina officinalis: cloning, expression, and refolding of the recombinant enzyme. Protein Expr Purif, 52(2), 265-272. Abstract.  Author URL. DOI.
Littlechild J, Garcia-Rodriguez E, Watts A, Coupe E, Isupov M (2007). The vanadium dependent bromoperoxidase enzymes:Insights into Mechanism and Applications gained from Structural Studies. In Aureliano M (Ed) Vanadium biochemistry, India: Signpost, 97-116.
Littlechild JA, Garcia-Rodriguez E, Watts A, Coupe E, Isupov M (2007). The vanadium dependent bromoperoxidase enzymes:Insights into Mechanism and Applications gained from Structural Studies. In Alves MA (Ed) Vanadium Biochemistry, India: Research Signpost, 97-116.
Talbot NJ, Littlechild JA, Wang Z-Y, Wilson RA (2007). Tps1 regulates the pentose phosphate pathway, nitrogen metabolism and fungal virulence. The EMBO Journal, 26, 3673-3685. DOI.
Wilson RA, Jenkinson JM, Gibson RP, Littlechild JA, Wang Z-Y, Talbot NJ (2007). Tps1 regulates the pentose phosphate pathway, nitrogen metabolism and fungal virulence. EMBO J, 26(15), 3673-3685. Abstract.  Author URL. DOI.

2006

Tapper S, Littlechild JA, Molard Y, Prokes I, Tucker JHR (2006). Anion binding tripodal receptors as structural models for the active site of vanadium haloperoxidases and acid phosphatases. Supramolecular Chemistry, 18(1), 55-58. Abstract. DOI.
Conklin PL, Gatzek S, Wheeler GL, Dowdle J, Raymond MJ, Rolinski S, Isupov M, Littlechild JA, Smirnoff N (2006). Arabidopsis thaliana VTC4 encodes L-galactose-1-P phosphatase, a plant ascorbic acid biosynthetic enzyme. J Biol Chem, 281(23), 15662-15670. Abstract.  Author URL. DOI.
Littlechild J, Garcia-Rodriguez E, Isupov M (2006). INOR 398-Crystallization studies of vanadium haloperoxidases: Insights into halide specificity and enzyme mechanism. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 232 Author URL.

2005

Garcia-Rodriguez E, Ohshiro T, Aibara T, Izumi Y, Littlechild J (2005). Enhancing effect of calcium and vanadium ions on thermal stability of bromoperoxidase from Corallina pilulifera. J Biol Inorg Chem, 10(3), 275-282. Abstract.  Author URL. DOI.
Littlechild J (2005). Nature's catalysts and their applications in the pharmaceutical industry. EBR - European Biopharmaceutical Review(AUTUMN), 64-68. Abstract.
Connelly S, Line K, Isupov MN, Littlechild JA (2005). Synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the Aureobacterium species (-) gamma-lactamase. Org Biomol Chem, 3(18), 3260-3262. Abstract.  Author URL. DOI.

2004

Taylor IN, Brown RC, Bycroft M, King G, Littlechild JA, Lloyd MC, Praquin C, Toogood HS, Taylor SJC (2004). Application of thermophilic enzymes in commercial biotransformation processes. England.  Abstract.  Author URL.
Clark DS, Ball P, Littlechild JA, Rice DW, Halle B (2004). Characteristics of nearly dry enzymes in organic solvents: Implications for biocatalysis in the absence of water. Philosophical Transactions of the Royal Society B: Biological Sciences, 359(1448), 1299-1307. Abstract. DOI.
Brice DC, Bryant JA, Dambrauskas G, Drury SC, Littlechild JA (2004). Cloning and expression of cytosolic phosphoglycerate kinase from pea (Pisum sativum L.). J Exp Bot, 55(398), 955-956. Abstract.  Author URL. DOI.
Isupov MN, Brindley AA, Hollingsworth EJ, Murshudov GN, Vagin AA, Littlechild JA (2004). Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. Acta Crystallogr D Biol Crystallogr, 60(Pt 10), 1879-1882. Abstract.  Author URL.  Full text. DOI.
Littlechild JA, Guy JE, Isupov MN (2004). Hyperthermophilic dehydrogenase enzymes. England.  Abstract.  Author URL.
Ohshiro T, Littlechild J, Garcia-Rodriguez E, Isupov MN, Iida Y, Kobayashi T, Izumi Y (2004). Modification of halogen specificity of a vanadium-dependent bromoperoxidase. Protein Sci, 13(6), 1566-1571. Abstract.  Author URL. DOI.
Rand RP, Ho MW, Littlechild JA, Finney JL, Cupane A, Engberts JBFN (2004). Probing the role of water in protein conformation and function. Philosophical Transactions of the Royal Society B: Biological Sciences, 359(1448), 1277-1285. Abstract. DOI.
Littlechild JA, Isupov MN, Line K (2004). The crystal structure of a (-)?-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site. Journal of Molecular Biology, 338(3), 519-532. DOI.
Toogood HS, Brown RC, Line K, Keene PA, Taylor SJC, McCague R, Littlechild JA (2004). The use of a thermostable signature amidase in the resolution of the bicyclic synthon (rac)-γ-lactam. Tetrahedron, 60(3), 711-716. Abstract. DOI.
Halling PJ, Finney JL, Ho MW, Franks F, Littlechild JA (2004). What can we learn by studying enzymes in non-aqueous media?. Philosophical Transactions of the Royal Society B: Biological Sciences, 359(1448), 1287-1297. Abstract. DOI.

2003

Whitcher CS, Littlechild JA, Woodley JM (2003). Application of a Baeyer-Villiger monooxygenase from cunninghamella echinulata NRRL 3655.  Author URL.
Guy JE, Isupov MN, Littlechild JA (2003). Crystallization and preliminary X-ray diffraction studies of a novel alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix. Acta Crystallogr D Biol Crystallogr, 59(Pt 1), 174-176. Abstract.  Author URL.  Full text.
Watts, A. Giles, G.I. Fry, F.H. (2003). Metal and Redox Regulation of Cysteine Protein Function. Chemistry and Biology, 10, 677-693.
Littlechild JA, Garcia-Rodriguez E (2003). Structural studies on the dodecameric vanadium bromoperoxidase from Corallina species. Coordination Chemistry Reviews, 237(1-2), 65-76. DOI.
Littlechild JA, Guy JE, Isupov MN (2003). The Structure of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix. Journal of Molecular Biology, 331(5), 1041-1051. DOI.

2002

Watts AB, Beecher J, Whitcher CS, Littlechild JA (2002). A method for screening Baeyer-Villiger monooxygenase activity against monocyclic ketones. Biocatalysis and Biotransformation, 20(3), 209-214. Abstract. DOI.
Toogood HS, Hollingsworth EJ, Brown RC, Taylor IN, Taylor SJC, McCague R, Littlechild JA (2002). A thermostable L-aminoacylase from Thermococcus litoralis: cloning, overexpression, characterization, and applications in biotransformations. Extremophiles, 6(2), 111-122. Abstract.  Author URL.
Hollingsworth EJ, Isupov MN, Littlechild JA (2002). Crystallization and preliminary X-ray diffraction analysis of L-aminoacylase from the hyperthermophilic archaeon Thermococcus litoralis. Acta Crystallogr D Biol Crystallogr, 58(Pt 3), 507-510. Abstract.  Author URL.  Full text.
Gloyn AL, Noordam K, Willemsen MA, Watts AB, Littlechild JA, Ellard S, Hatterlsey AT (2002). Familial hyperinsulinism caused by a novel activating glucokinase mutation. DIABETES, 51, A260-A260. Author URL.
Toogood HS, Taylor IN, Brown RC, Taylor SJC, McCague R, Littlechild JA (2002). Immobilisation of the thermostable L-aminoacylase from Thermococcus litoralis to generate a reusable industrial biocatalyst. Biocatalysis and Biotransformation, 20(4), 241-249. Abstract. DOI.
Littlechild J, Garcia-Rodriguez E, Dalby A, Isupov M (2002). Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes. J Mol Recognit, 15(5), 291-296. Abstract.  Author URL. DOI.

2001

Qi JF, Isupov MN, Littlechild JA, Anderson LE (2001). Chloroplast glyceraldehyde-3-P dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. BIOCHEMISTRY, 40(29), 8615-8615. Author URL.
Qi JF, Isupov MN, Littlechild JA, Anderson LE (2001). Chloroplast glyceraldehyde-3-P dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 222, U121-U121. Author URL.
Littlechild JA, Anderson LE, Isupov MN, Qi JF (2001). Chloroplast glyceraldehydes-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. Journal of Biological Chemistry, 276(38), 35247-35252. DOI.
Harris JR, Schröder E, Isupov MN, Scheffler D, Kristensen P, Littlechild JA, Vagin AA, Meissner U (2001). Comparison of the decameric structure of peroxiredoxin-II by transmission electron microscopy and X-ray crystallography. Biochim Biophys Acta, 1547(2), 221-234. Abstract.  Author URL.
Gonsalvez IS, Isupov MN, Littlechild JA (2001). Crystallization and preliminary X-ray analysis of a gamma-lactamase. Acta Crystallogr D Biol Crystallogr, 57(Pt 2), 284-286. Abstract.  Author URL.  Full text.
Turner NA, Gaskin DJ, Yagnik AT, Littlechild JA, Vulfson EN (2001). Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones. Protein Eng, 14(4), 269-278. Abstract.  Author URL.
Littlechild JA, Isupov M (2001). Glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus solfataricus. Methods Enzymol, 331, 105-117. Author URL.
Crowhurst G, McHarg J, Littlechild JA (2001). Phosphoglycerate kinases from bacteria and archaea. Methods Enzymol, 331, 90-104. Author URL.
Singleton MR, Littlechild JA (2001). Pyrrolidone carboxylpeptidase from Thermococcus litoralis. Methods Enzymol, 330, 394-403. Author URL.
Littlechild J, Isupov M, Garcia E (2001). Structural studies on the dodecameric vanadium containing bromoperoxidase from Corallina officinalis. JOURNAL OF INORGANIC BIOCHEMISTRY, 86(1), 67-67. Author URL.
Dalby AR, Tolan DR, Littlechild JA (2001). The structure of human liver fructose-1,6-bisphosphate aldolase. Acta Crystallogr D Biol Crystallogr, 57(Pt 11), 1526-1533. Abstract.  Author URL.

2000

Singleton MR, Taylor SJ, Parrat JS, Littlechild JA (2000). Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis. Extremophiles, 4(5), 297-303. Abstract.  Author URL.
Schröder E, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Isupov MN (2000). Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 a resolution. Structure, 8(6), 605-615. Abstract.  Author URL.
Schroder E, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Isupov MN (2000). Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 angstrom resolution (vol 8, pg 605, 2000). STRUCTURE, 8(12), U5-U5. Author URL.
Isupov MN, Dalby AR, Brindley AA, Izumi Y, Tanabe T, Murshudov GN, Littlechild JA (2000). Crystal structure of dodecameric vanadium-dependent bromoperoxidase from the red algae Corallina officinalis. J Mol Biol, 299(4), 1035-1049. Abstract.  Author URL. DOI.
Bourne PC, Isupov MN, Littlechild JA (2000). The atomic-resolution structure of a novel bacterial esterase. Structure, 8(2), 143-151. Abstract.  Author URL.
Charron C, Talfournier F, Isupov MN, Littlechild JA, Branlant G, Vitoux B, Aubry A (2000). The crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus in the presence of NADP(+) at 2.1 a resolution. J Mol Biol, 297(2), 481-500. Abstract.  Author URL. DOI.

1999

Dalby A, Dauter Z, Littlechild JA (1999). Crystal structure of human muscle aldolase complexed with fructose 1,6-bisphosphate: mechanistic implications. Protein Sci, 8(2), 291-297. Abstract.  Author URL. DOI.
Isupov MN, Fleming TM, Dalby AR, Crowhurst GS, Bourne PC, Littlechild JA (1999). Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. J Mol Biol, 291(3), 651-660. Abstract.  Author URL. DOI.
Schröder E, Isupov MN, Naran A, Littlechild JA (1999). Crystallization and preliminary X-ray analysis of human thioredoxin peroxidase-B from red blood cells. Acta Crystallogr D Biol Crystallogr, 55(Pt 2), 536-538. Abstract.  Author URL.  Full text.
Charron C, Talfournier F, Isupov MN, Branlant G, Littlechild JA, Vitoux B, Aubry A (1999). Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus. Acta Crystallogr D Biol Crystallogr, 55(Pt 7), 1353-1355. Abstract.  Author URL.  Full text.
Singleton MR, Isupov MN, Littlechild JA (1999). Crystallization and preliminary X-ray diffraction studies of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Acta Crystallogr D Biol Crystallogr, 55(Pt 3), 702-703. Abstract.  Author URL.  Full text.
Bourne PC, Isupov MN, Littlechild JA (1999). Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase. Acta Crystallogr D Biol Crystallogr, 55(Pt 4), 915-917. Abstract.  Author URL.  Full text.
Littlechild J (1999). Haloperoxidases and their role in biotransformation reactions. Curr Opin Chem Biol, 3(1), 28-34. Abstract.  Author URL.
McHarg J, Kelly SM, Price NC, Cooper A, Littlechild JA (1999). Site-directed mutagenesis of proline 204 in the 'hinge' region of yeast phosphoglycerate kinase. Eur J Biochem, 259(3), 939-945. Abstract.  Author URL.
Crowhurst GS, Dalby AR, Isupov MN, Campbell JW, Littlechild JA (1999). Structure of a phosphoglycerate mutase:3-phosphoglyceric acid complex at 1.7 A. Acta Crystallogr D Biol Crystallogr, 55(Pt 11), 1822-1826. Abstract.  Author URL.  Full text.
Singleton M, Isupov M, Littlechild J (1999). X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Structure, 7(3), 237-244. Abstract.  Author URL.

1998

Fleming TM, Jones CE, Piper PW, Cowan DA, Isupov MN, Littlechild JA (1998). Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. Acta Crystallogr D Biol Crystallogr, 54(Pt 4), 671-674. Abstract.  Author URL.  Full text.
McGhie EJ, Isupov MN, Schröder E, Littlechild JA (1998). Crystallization and preliminary X-ray diffraction studies of the oxygenating subunit of 3,6-diketocamphane monooxygenase from Pseudomonas putida. Acta Crystallogr D Biol Crystallogr, 54(Pt 5), 1035-1038. Abstract.  Author URL.  Full text.
Brindley AA, Dalby AR, Isupov MN, Littlechild JA (1998). Preliminary X-ray analysis of a new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis. Acta Crystallogr D Biol Crystallogr, 54(Pt 3), 454-457. Abstract.  Author URL.  Full text.
Crowhurst GS, Isupov MN, Fleming T, Littlechild JA (1998). Two glycolytic enzymes from Sulfolobus solfataricus. England.  Author URL.

1997

Lewis G, Bevan J, Rawas A, McMichael P, Wisdom R, McCague R, Watson H, Littlechild J (1997). Crystallization and preliminary X-ray studies on Candida cylindracea lipase. Acta Crystallogr D Biol Crystallogr, 53(Pt 3), 348-351. Abstract.  Author URL. DOI.
Yagnik AT, Littlechild JA, Turner NJ (1997). Molecular modelling studies of substrate binding to the lipase from Rhizomucor miehei. J Comput Aided Mol Des, 11(3), 256-264. Abstract.  Author URL.
Bentley PA, Kroutil W, Littlechild JA, Roberts SM (1997). Preparation of polyamino acid catalysts for use in Julia asymmetric epoxidation. Chirality, 9(2), 198-202. Abstract.
Fleming T, Littlechild J (1997). Sequence and structural comparison of thermophilic phosphoglycerate kinases with a mesophilic equivalent. Comp Biochem Physiol a Physiol, 118(3), 439-451. Abstract.  Author URL.
Isupov MN, Obmolova G, Butterworth S, BadetDenisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A (1997). Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: Evidence from the 1.8 angstrom crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase (vol 4, pg 801, 1996). STRUCTURE, 5(5), 723-723. Author URL.

1996

Lilly MD, Chauhan R, French C, Gyamerah M, Hobbs GR, Humphrey A, Isupov M, Littlechild JA, Mitra RK, Morris KG, et al (1996). Carbon-carbon bond synthesis: the impact of rDNA technology on the production and use of E. coli transketolase.  Abstract. DOI.
Piper PW, Emson C, Jones CE, Cowan DA, Fleming TM, Littlechild JA (1996). Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic Archaeon Sulfolobus solfataricus. Curr Genet, 29(6), 594-596. Abstract.  Author URL.
Dalby A, Rawas A, Watson HC, Littlechild JA (1996). Crystallisation and preliminary x-ray diffraction studies on human liver aldolase. Protein and Peptide Letters, 3(3), 207-212. Abstract.
Brabban AD, Littlechild J, Wisdom R (1996). Stereospecific γ-lactamase activity in a Pseudomonas fluorescens species. Journal of Industrial Microbiology, 16(1), 8-14. Abstract. DOI.
Mcharg J, Littlechild JA (1996). Studies with inhibitors of the glycolytic enzyme phosphoglycerate kinase for potential treatment of cardiovascular and respiratory disorders. J Pharm Pharmacol, 48(2), 201-205. Abstract.  Author URL.
Dalby A, Littlechild JA (1996). Studies with type I aldolase to understand fructose intolerance and combat parasitic disease. J Pharm Pharmacol, 48(2), 214-217. Abstract.  Author URL.
Isupov MN, Obmolova G, Butterworth S, Badet-Denisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A (1996). Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: evidence from the 1.8 a crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase. Structure, 4(7), 801-810. Abstract.  Author URL.
McGhie EJ, Littlechild JA (1996). The purification and crystallisation of 2,5-diketocamphane 1,2 monooxygenase and 3,6-diketocamphane 1,6 monooxygenase from Pseudomonas putida NCIMB 10007.
McGhie EJ, Littlechild JA (1996). The purification and crystallisation of 2,5-diketocamphane 1,2-monooxygenase and 3,6-diketocamphane 1,6-monooxygenase from Pseudomonas putida NCIMB 10007. Biochem Soc Trans, 24(1). Author URL.
Fleming TM, Jones CE, Piper PW, Cowan DA, Adams MWW, Littlechild JA (1996). The purification and crystallisation of phosphoglycerate kinase from the hyperthermophilic eubacterium thermotoga maritima. Protein and Peptide Letters, 3(3), 213-218. Abstract.

1995

Jones CE, Fleming TM, Piper PW, Littlechild JA, Cowan DA (1995). Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli. Gene, 160(1), 101-103. Abstract.  Author URL.
Littlechild J, Turner N, Hobbs G, Lilly M, Rawas A, Watson H (1995). Crystallization and preliminary X-ray crystallographic data with Escherichia coli transketolase. Acta Crystallogr D Biol Crystallogr, 51(Pt 6), 1074-1076. Abstract.  Author URL. DOI.
Rush C, Willetts A, Davies G, Dauter Z, Watson H, Littlechild J (1995). Purification, crystallisation and preliminary X-ray analysis of the vanadium-dependent haloperoxidase from Corallina officinalis. FEBS Lett, 359(2-3), 244-246. Abstract.  Author URL.
Jones CE, Fleming TM, Cowan DA, Littlechild JA, Piper PW (1995). The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli. Eur J Biochem, 233(3), 800-808. Abstract.  Author URL.

1994

Davies GJ, Gamblin SJ, Littlechild JA, Dauter Z, Wilson KS, Watson HC (1994). Structure of the ADP complex of the 3-phosphoglycerate kinase from Bacillus stearothermophilus at 1.65 A. Acta Crystallogr D Biol Crystallogr, 50(Pt 2), 202-209. Abstract.  Author URL. DOI.

1993

Littlechild JA, Watson HC (1993). A data-based reaction mechanism for type I fructose bisphosphate aldolase. Trends Biochem Sci, 18(2), 36-39. Abstract.  Author URL.
Taylor SJ, McCague R, Wisdom R, Lee C, Dickson K, Ruecroft G, O'Brien F, Littlechild J, Bevan J, Roberts SM, et al (1993). Development of the biocatalytic resolution of 2-azabicyclo[2.2.1]hept-5-en-3-one as an entry to single-enantiomer carbocyclic nucleosides. Tetrahedron: Asymmetry, 4(6), 1117-1128. Abstract. DOI.
BARBER MD, GAMBLIN SJ, WATSON HC, LITTLECHILD JA (1993). SITE-DIRECTED MUTAGENESIS OF YEAST PHOSPHOGLYCERATE KINASE - ARGININE-65, ARGININE-121 AND ARGININE-168. FEBS LETTERS, 320(3), 193-197. Author URL. DOI.
Vellieux FM, Hajdu J, Verlinde CL, Groendijk H, Read RJ, Greenhough TJ, Campbell JW, Kalk KH, Littlechild JA, Watson HC, et al (1993). Structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei determined from Laue data. Proc Natl Acad Sci U S A, 90(6), 2355-2359. Abstract.  Author URL.
Davies GJ, Gamblin SJ, Littlechild JA, Watson HC (1993). The structure of a thermally stable 3-phosphoglycerate kinase and a comparison with its mesophilic equivalent. Proteins, 15(3), 283-289. Abstract.  Author URL. DOI.

1992

Joao HC, Williams RJ, Littlechild JA, Nagasuma R, Watson HC (1992). An investigation of large inhibitors binding to phosphoglycerate kinase and their effect on anion activation. Eur J Biochem, 205(3), 1077-1088. Abstract.  Author URL.
Walker PA, Joâo HC, Littlechild JA, Williams RJ, Watson HC (1992). Characterisation of yeast phosphoglycerate kinase modified by mutagenesis at residue 21. Eur J Biochem, 207(1), 29-37. Abstract.  Author URL.
Eisenberg D, Perutz MF, Buckingham AD, Graf L, Thornton J, Blow DM, Fersht AR, Karplus M, Johnson LN, Rippmann F, et al (1992). General discussion. Faraday Discussions, 93, 107-129. DOI.
Davies GJ, Gamblin SJ, Littlechild JA, Watson HC (1992). Purification, crystallization and preliminary X-ray analysis of the 3-phosphoglycerate kinase from Bacillus stearothermophilus. J Mol Biol, 227(4), 1263-1264. Abstract.  Author URL.

1991

Graham HC, Williams RJ, Littlechild JA, Watson HC (1991). A proton-NMR study of a site-directed mutation (His388----Glu) in the interdomain region of yeast phosphoglycerate kinase. Implications for domain movement. Eur J Biochem, 196(2), 261-269. Abstract.  Author URL.
Gamblin SJ, Davies GJ, Grimes JM, Jackson RM, Littlechild JA, Watson HC (1991). Activity and specificity of human aldolases. J Mol Biol, 219(4), 573-576. Abstract.  Author URL.
Littlechild JA (1991). Protein crystallization: Magical or logical: can we establish some general rules?. Journal of Physics D: Applied Physics, 24(2), 111-118. Abstract. DOI.
Davies GJ, Littlechild JA, Watson HC, Hall L (1991). Sequence and expression of the gene encoding 3-phosphoglycerate kinase from Bacillus stearothermophilus. Gene, 109(1), 39-45. Abstract.  Author URL.

1990

Watson HC, Littlechild JA (1990). Isoenzymes of phosphoglycerate kinase: evolutionary conservation of the structure of this glycolytic enzyme. England.  Author URL.
Minard P, Bowen DJ, Hall L, Littlechild JA, Watson HC (1990). Site-directed mutagenesis of aspartic acid 372 at the ATP binding site of yeast phosphoglycerate kinase: over-expression and characterization of the mutant enzyme. Protein Eng, 3(6), 515-521. Abstract.  Author URL.
Gamblin SJ, Cooper B, Millar JR, Davies GJ, Littlechild JA, Watson HC (1990). The crystal structure of human muscle aldolase at 3.0 a resolution. FEBS Lett, 262(2), 282-286. Abstract.  Author URL.

1989

Fairbrother WJ, Walker PA, Minard P, Littlechild JA, Watson HC, Williams RJ (1989). NMR analysis of site-specific mutants of yeast phosphoglycerate kinase. An investigation of the triose-binding site. Eur J Biochem, 183(1), 57-67. Abstract.  Author URL.
Fairbrother WJ, Hall L, Littlechild JA, Walker PA, Watson HC, Williams RJ (1989). Site-directed mutagenesis of histidine 62 in the 'basic patch' region of yeast phosphoglycerate kinase. FEBS Lett, 258(2), 247-250. Abstract.  Author URL.
Walker PA, Littlechild JA, Hall L, Watson HC (1989). Site-directed mutagenesis of yeast phosphoglycerate kinase. The 'basic-patch' residue arginine 168. Eur J Biochem, 183(1), 49-55. Abstract.  Author URL.

1988

Wilson HR, Williams RJ, Littlechild JA, Watson HC (1988). NMR analysis of the interdomain region of yeast phosphoglycerate kinase. Eur J Biochem, 170(3), 529-538. Abstract.  Author URL.
Bowen D, Littlechild JA, Fothergill JE, Watson HC, Hall L (1988). Nucleotide sequence of the phosphoglycerate kinase gene from the extreme thermophile Thermus thermophilus. Comparison of the deduced amino acid sequence with that of the mesophilic yeast phosphoglycerate kinase. Biochem J, 254(2), 509-517. Abstract.  Author URL.
FAIRBROTHER WJ, HALL L, LITTLECHILD JA, WALKER PA, WATSON HC, WILLIAMS RJP (1988). PROBING THE 3-PHOSPHOGLYCERATE-BINDING SITE OF YEAST PHOSPHOGLYCERATE KINASE USING SITE-SPECIFIC MUTANTS AND H-1 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY. BIOCHEMICAL SOCIETY TRANSACTIONS, 16(5), 724-725. Author URL.

1987

FAIRBROTHER WJ, HALL L, LITTLECHILD J, MINARD P, WATSON HC, WILLIAMS RJP (1987). ANION BINDING STUDY OF YEAST PHOSPHOGLYCERATE KINASE BY NMR AND SITE-SPECIFIC MUTAGENESIS. PROTEIN ENGINEERING, 1(3), 261-261. Author URL.
FAIRBROTHER WJ, HALL L, LITTLECHILD JA, MINARD P, WATSON HC, WILLIAMS RJP (1987). ANION BINDING STUDY OF YEAST PHOSPHOGLYCERATE KINASE BY NUCLEAR-MAGNETIC-RESONANCE AND SITE-SPECIFIC MUTAGENESIS. BIOCHEMICAL SOCIETY TRANSACTIONS, 15(5), 868-869. Author URL.
BOWEN D, DAVIES G, GAMBLIN S, HALL L, LITTLECHILD J, WATSON HC (1987). PHOSPHOGLYCERATE KINASE - a TEST-BED SYSTEM FOR THE STUDY OF PROTEIN THERMOSTABILITY. PROTEIN ENGINEERING, 1(3), 242-242. Author URL.
Littlechild JA, Davies GJ, Gamblin SJ, Watson HC (1987). Phosphoglycerate kinase from the extreme thermophile Thermus thermophilus Crystallization and preliminary X-ray data. FEBS Letters, 225(1-2), 123-126. Abstract. DOI.
MINARD P, WALKER P, BOWEN D, DAVIES G, LITTLECHILD J, HALL L, WATSON HC (1987). THE USE OF SITE-DIRECTED MUTAGENESIS TO STUDY THE GLYCOLYTIC ENZYME PHOSPHOGLYCERATE KINASE. PROTEIN ENGINEERING, 1(3), 260-260. Author URL.
Littlechild J, Malcolm A, Paterakis K, Ackermann I, Dijk J (1987). The tertiary structure of salt-extracted ribosomal proteins from Escherichia coli as studied by proton magnetic resonance spectroscopy and limited proteolysis experiments. Biochim Biophys Acta, 913(2), 245-255. Abstract.  Author URL.

1986

Crout DHG, Littlechild J, Morrey SM (1986). Acetoin metabolism: Stereochemistry of the acetoin produced by the pyruvate decarboxylase of wheat germ and by the α-acetolactate decarboxylase of Klebsiella aerogenes. Journal of the Chemical Society, Perkin Transactions 1, 105-108. Abstract.
LITTLECHILD JA (1986). THE TYPE-II RESTRICTION ENZYMES HGIAI AND TAQI - PURIFICATION AND PROPERTIES. BIOCHEMICAL SOCIETY TRANSACTIONS, 14(2), 268-268. Author URL.
Dijk J, Littlechild JA, Freund AM, Pouyet J, Daune M, Provencher SW (1986). The secondary structure of salt-extracted ribosomal proteins from Escherichia coli as studied by circular dichroic spectroscopy. Biochim Biophys Acta, 874(2), 227-234. Abstract.  Author URL.

1984

Stöffler-Meilicke M, Epe B, Woolley P, Lotti M, Littlechild J, Stöffler G (1984). Location of protein S4 on the small ribosomal subunit of E. coli and B. stearothermophilus with protein- and hapten-specific antibodies. Mol Gen Genet, 197(1), 8-18. Abstract.  Author URL.
Crout DHG, Littlechild J, Mitchell MB, Morrey SM (1984). Stereochemistry of the decarboxylation of α-acetolactate (2-hydroxy-2-methyl-3-oxobutanoate) by the acetolactate decarboxylase of Klebsiella aerogenes. Journal of the Chemical Society, Perkin Transactions 1, 2271-2276. Abstract.

1983

Paterakis K, Littlechild J, Woolley P (1983). Structural and functional studies on protein S20 from the 30-S subunit of the Escherichia coli ribosome. Eur J Biochem, 129(3), 543-548. Abstract.  Author URL.

1982

Epe B, Woolley P, Steinhäuser KG, Littlechild J (1982). Distance measurement by energy transfer: the 3' end of 16-S RNA and proteins S4 and S17 of the ribosome of Escherichia coli. Eur J Biochem, 129(1), 211-219. Abstract.  Author URL.
Wirth R, Littlechild J, Böck A (1982). Ribosomal protein S20 purified under mild conditions almost completely inhibits its own translation. Mol Gen Genet, 188(1), 164-166. Abstract.  Author URL.
Paterakis K, Littlechild J (1982). Structural domains of ribosomal protein S8 and their relationship to ribosomal RNA binding. FEBS Lett, 149(2), 328-333. Abstract.  Author URL.

1981

Georgalis Y, Giri L, Littlechild JA (1981). Physical studies on the ribosomal protein S2 from the Escherichia coli 30S. Biochemistry, 20(5), 1061-1064. Abstract.  Author URL.
Pawlik RT, Littlechild J, Pon C, Gualerzi C (1981). Purification and properties of Escherichia coli translational initiation factors. Biochemistry International, 2(4), 421-428.

1980

LITTLECHILD JA, MALCOLM AL, SOWDEN BK (1980). COMPARATIVE STUDIES ON THE 30S RIBOSOMAL-PROTEINS FROM ESCHERICHIA-COLI AND BACILLUS-STEAROTHERMOPHILUS. EUROPEAN JOURNAL OF CELL BIOLOGY, 22(1), 133-133. Author URL.
Littlechild J (1980). Proton magnetic resonance studies of Escherichia coli ribosomal protein S16. FEBS Lett, 111(1), 51-55. Author URL.
LITTLECHILD JA, PATERAKIS K (1980). STRUCTURAL STABILITY OF SEVERAL PROTEINS ISOLATED FROM THE 30S-RIBOSOMAL SUBUNIT OF ESCHERICHIA-COLI. EUROPEAN JOURNAL OF CELL BIOLOGY, 22(1), 132-132. Author URL.

1979

Littlechild J, Morrison CA, Bradbury EM (1979). Proton magnetic resonance studies of Escherichia coli ribosomal protein S4 and a C-terminal fragment of this protein. FEBS Lett, 104(1), 90-94. Author URL.
Dijk J, Littlechild J (1979). Purification of ribosomal proteins from Escherichia coli under nondenaturing conditions. Methods Enzymol, 59, 481-502. Author URL.

1978

Khechinashvili NN, Koteliansky VE, Gogia ZV, Littlechild J, Dijk J (1978). A heat denaturation study of several ribosomal proteins from Escherichia coli by scanning microcalorimetry. FEBS Letters, 95(2), 270-272. DOI.
Khechinashvili NN, Koteliansky VE, Gogia ZV, Littlechild J, Dijk J (1978). A heat denaturation study of several ribosomal proteins from Escherichia coli by scanning microcalorimetry. FEBS Lett, 95(2), 270-272. Author URL.
Littlechild JA, Malcolm AL (1978). A new method for the purification of 30S ribosomal proteins from Escherichia coli using nondenaturing conditions. Biochemistry, 17(16), 3363-3369. Abstract.  Author URL.
OSTERBERG R, SJOBERG B, LILJAS A, GARRETT RA, LITTLECHILD J (1978). SMALL-ANGLE X-RAY-SCATTERING STUDY OF COMPLEXES OF INDIVIDUAL COMPONENTS FROM ESCHERICHIA-COLI RIBOSOMES. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 11(OCT), 487-487. Author URL. DOI.
Österberg R, Sjöberg B, Littlechild J (1978). Small-angle X-ray scattering study of the proteins S1, S8, S15, S16, S20 from Escherichia coli ribosomes. FEBS Letters, 93(1), 115-119. DOI.
Osterberg R, Sjöberg B, Garrett RA, Littlechild J (1978). Small-angle X-ray titration of the complex formed between the ribosomal protein S4 and its 16S binding site, S4-RNA: a central core in the 30S subunit. Nucleic Acids Res, 5(10), 3579-3587. Abstract.  Author URL.

1977

Giri L, Littlechild J, Dijk J (1977). Hydrodynamic studies on the Escherichia coli robosomal proteins S8 and L6, prepared by two different methods. FEBS Lett, 79(2), 238-244. Author URL.
Morrison CA, Bradbury EM, Littlechild J, Dijk J (1977). Proton magnetic resonance studies to compare Escherichia coli ribosomal proteins prepared by two different methods. FEBS Lett, 83(2), 348-352. Author URL.
Österberg R, Sjöberg B, Garrett RA, Littlechild J (1977). Small-angle X-ray scattering study of the 16 S RNA binding protein S4 from Escherichia coli ribosomes. FEBS Letters, 73(1), 22-24. DOI.
Osterberg R, Sjöberg B, Garrett RA, Littlechild J (1977). Small-angle x-ray scattering study of the 16S RNA binding protein S4 from Escherichia coli ribosomes. FEBS Lett, 73(1), 25-28. Author URL.
Dijk J, Littlechild J, Garrett RA (1977). The RNA binding properties of "native" protein-protein complexes isolated from the Escherichia coli ribosome. FEBS Lett, 77(2), 295-300. Author URL.
Littlechild J, Dijk J, Garrett RA (1977). The identification of new RNA-binding proteins in the Escherichia coli ribosome. FEBS Lett, 74(2), 292-294. Author URL.

1973

Littlechild J (1973). Nuclease contaminant of a batch of lysozyme used for ribosome extraction: cleavage of Escherichia coli 23S rRNA into two specific fragments. Anal Biochem, 55(2), 634-636. Author URL.
Littlechild J, Spencer M (1973). Stability and homogeneity of preparations of ribosomal particles from Escherichia coli. Biochemistry, 12(16), 3102-3108. Author URL.