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Dr Leandro Jose de Assis

Dr Leandro Jose de Assis

Postdoctoral Research RA

 Geoffrey Pope 328

 

Geoffrey Pope Building, University of Exeter , Stocker Road, Exeter, EX4 4QD, UK

Overview

My main research focuses on fungal biochemistry, genetics and metabolism, more specifically, in the characterization of carbon source metabolism. I have been dedicated to understand and elucidate mechanisms governing glucose signaling. I have gained skills in basic and advanced microbiology, biochemistry and genetics techniques from different universities: (i) Federal University of Lavras (Minas Gerais, Brazil) in basic microbiology methods for agricultural applications, (ii) Institute of Medical Biochemistry at the Federal University of Rio de Janeiro (Rio de Janeiro, Brazil) in basic and advanced biochemical and metabolism techniques and (iii) University of São Paulo (São Paulo, Brazil) in advanced molecular biology and genetics methodologies. As part of my post-doctoral fellowship, gained experience working with genetics and proteomics at Maynooth University in Ireland, also I was working as a post-doctoral fellow at the Georg-August University of Gottingen in Germany applying genetics and proteomics approach. I therefore have good knowledge in assaying the activities of a wide range of enzymes, characterizing interactions between genes as well as protein physical interactions using techniques such as immunoprecipitation and know how to do a screening of strains (yeast and filamentous fungi) for drugs using microplate reader. I am currently working on various aspects of sugar metabolism studying cell wall construction and the exposition of carbohydrates on the surface of the fungi to the host.

 

Qualifications

Bachelor in Biological science (agricultural applications)

Bachelor in Biomedicine

Ph.D.: Chemical Biology

Postdoctoral fellow: Biochemistry, Genetics, Metabolism, and Proteomics.

 

Career

2003-2005 Biological Science, Federal University of Lavras, Minas Gerais, Brazil.

2005-2009 Biomedicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

2009-2013 Ph.D. Chemical Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

2016-2016 Postdoctoral fellowship, Maynooth University, Ireland,

2018-2018 Postdoctoral fellowship, Georg-August University of Gottingen, Germany.

2013-2019 Postdoctoral fellowship, University of São Paulo, São Paulo, Brazil.

Research group links

Research

Research interests

My main research focuses on fungal biochemistry, genetics, and metabolism, more specifically, in the characterization of carbon source metabolism. I have been dedicated to understand and elucidate mechanisms governing glucose signaling.  I am currently working on various aspects of sugar metabolism studying cell wall construction and the exposition of carbohydrates on the surface of the fungi to the host.

Awards

Cover Illustration mBio November/December Volume 9, N˚ 6 (de Assis et al., mBio 9:e01952-18, 2018), American Society of Microbiology (ASM).

Patent

2014   Engineered yeast strain that reduces the production of foam during fermentation processes, Patent number: BR10201400268, National Institute of Intellectual Property, Brazil.

 

Research grants

  • 2020 Marie Curie Fellowship
    Independent researcher Marie-Curie MSCA-IF-EF-ST H2020 94.40% score (Nuclear Regulators of Fungal Enzymes), National University of Ireland - Maynooth.

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Prusky D, de Assis LJ, Baroncelli R, Benito EP, del Castillo VC, Chaya T, Covo S, Díaz-Mínguez JM, Donofrio NM, Espeso E, et al (2020). Nutritional factors modulating plant and fruit susceptibility to pathogens: BARD workshop, Haifa, Israel, February 25–26, 2018. Phytoparasitica, 48(3), 317-333.
Antonieto ACC, Nogueira KMV, de Paula RG, Nora LC, Cassiano MHA, Guazzaroni M-E, Almeida F, da Silva TA, Ries LNA, de Assis LJ, et al (2019). A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei. mSystems, 4(4). Abstract.
Manfiolli AO, Mattos EC, de Assis LJ, Silva LP, Ulaş M, Brown NA, Silva-Rocha R, Bayram Ö, Goldman GH (2019). Aspergillus fumigatus High Osmolarity Glycerol Mitogen Activated Protein Kinases SakA and MpkC Physically Interact During Osmotic and Cell Wall Stresses. Frontiers in Microbiology, 10
Horta MAC, Thieme N, Gao Y, Burnum-Johnson KE, Nicora CD, Gritsenko MA, Lipton MS, Mohanraj K, de Assis LJ, Lin L, et al (2019). Broad Substrate-Specific Phosphorylation Events Are Associated with the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa. Frontiers in Microbiology, 10
Ribeiro LFC, Chelius C, Boppidi KR, Naik NS, Hossain S, Ramsey JJJ, Kumar J, Ribeiro LF, Ostermeier M, Tran B, et al (2019). Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation. mBio, 10(2). Abstract.
Suriani Ribeiro M, Graciano de Paula R, Raquel Voltan A, de Castro RG, Carraro CB, José de Assis L, Stecca Steindorff A, Goldman GH, Silva RN, Ulhoa CJ, et al (2019). Endo-β-1,3-glucanase (GH16 Family) from Trichoderma harzianum Participates in Cell Wall Biogenesis but is Not Essential for Antagonism Against Plant Pathogens. Biomolecules, 9(12), 781-781. Abstract.  Full text.
Manfiolli AO, Siqueira FS, dos Reis TF, Van Dijck P, Schrevens S, Hoefgen S, Föge M, Straßburger M, de Assis LJ, Heinekamp T, et al (2019). Mitogen-Activated Protein Kinase Cross-Talk Interaction Modulates the Production of Melanins in Aspergillus fumigatus. mBio, 10(2). Abstract.
Ries LNA, Steenwyk JL, de Castro PA, de Lima PBA, Almeida F, de Assis LJ, Manfiolli AO, Takahashi-Nakaguchi A, Kusuya Y, Hagiwara D, et al (2019). Nutritional Heterogeneity Among Aspergillus fumigatus Strains Has Consequences for Virulence in a Strain- and Host-Dependent Manner. Frontiers in Microbiology, 10
Manfiolli AO, dos Reis TF, de Assis LJ, de Castro PA, Silva LP, Hori JI, Walker LA, Munro CA, Rajendran R, Ramage G, et al (2018). Mitogen activated protein kinases (MAPK) and protein phosphatases are involved in Aspergillus fumigatus adhesion and biofilm formation. The Cell Surface, 1, 43-56.
de Assis LJ, Manfiolli A, Mattos E, Fabri JHTM, Malavazi I, Jacobsen ID, Brock M, Cramer RA, Thammahong A, Hagiwara D, et al (2018). Protein Kinase a and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus. mBio, 9(6). Abstract.
de Assis LJ, Ulas M, Ries LNA, El Ramli NAM, Sarikaya-Bayram O, Braus GH, Bayram O, Goldman GH (2018). Regulation of. Aspergillus nidulans. CreA-Mediated Catabolite Repression by the F-Box Proteins Fbx23 and Fbx47. mBio, 9(3). Abstract.
Ries LNA, José de Assis L, Rodrigues FJS, Caldana C, Rocha MC, Malavazi I, Bayram Ö, Goldman GH (2018). The Aspergillus nidulans Pyruvate Dehydrogenase Kinases Are Essential to Integrate Carbon Source Metabolism. G3: Genes|Genomes|Genetics, 8(7), 2445-2463.
Ries LNA, Rocha MC, de Castro PA, Silva-Rocha R, Silva RN, Freitas FZ, de Assis LJ, Bertolini MC, Malavazi I, Goldman GH, et al (2017). The Aspergillus fumigatus CrzA Transcription Factor Activates Chitin Synthase Gene Expression during the Caspofungin Paradoxical Effect. mBio, 8(3). Abstract.
dos Reis TF, Nitsche BM, de Lima PBA, de Assis LJ, Mellado L, Harris SD, Meyer V, dos Santos RAC, Riaño-Pachón DM, Ries LNA, et al (2017). The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans. Scientific Reports, 7(1).
Bruder Nascimento ACMDO, dos Reis TF, de Castro PA, Hori JI, Bom VLP, de Assis LJ, Ramalho LNZ, Rocha MC, Malavazi I, Brown NA, et al (2016). Mitogen activated protein kinases SakAHOG1and MpkC collaborate forAspergillus fumigatusvirulence. Molecular Microbiology, 100(5), 841-859.
de Assis LJ, Ries LNA, Savoldi M, dos Reis TF, Brown NA, Goldman GH (2015). Aspergillus nidulans protein kinase a plays an important role in cellulase production. Biotechnology for Biofuels, 8(1).
de Assis LJ, Ries LNA, Savoldi M, Dinamarco TM, Goldman GH, Brown NA (2015). Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism inAspergillus nidulans. G3: Genes|Genomes|Genetics, 5(5), 857-872.
de Assis LJ, Zingali RB, Masuda CA, Rodrigues SP, Montero-Lomelí M (2013). Pyruvate decarboxylase activity is regulated by the Ser/Thr protein phosphatase Sit4p in the yeastSaccharomyces cerevisiae. FEMS Yeast Research, 13(6), 518-528.
Masuda CA, Previato JO, Miranda MN, Assis LJ, Penha LL, Mendonça-Previato L, Montero-Lomelí MN (2008). Overexpression of the aldose reductaseGRE3 suppresses lithium-induced galactose toxicity inSaccharomyces cerevisiae. FEMS Yeast Research, 8(8), 1245-1253.
Tomizawa MM, Dias ES, Assis LJD, Gomide PHO, Santos JBD (2007). Variabilidade genética de isolados do cogumelo Agaricus blazei por meio de marcadores RAPD. Ciência e Agrotecnologia, 31(4), 1242-1249. Abstract.

Publications by year


2020

Prusky D, de Assis LJ, Baroncelli R, Benito EP, del Castillo VC, Chaya T, Covo S, Díaz-Mínguez JM, Donofrio NM, Espeso E, et al (2020). Nutritional factors modulating plant and fruit susceptibility to pathogens: BARD workshop, Haifa, Israel, February 25–26, 2018. Phytoparasitica, 48(3), 317-333.

2019

Antonieto ACC, Nogueira KMV, de Paula RG, Nora LC, Cassiano MHA, Guazzaroni M-E, Almeida F, da Silva TA, Ries LNA, de Assis LJ, et al (2019). A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei. mSystems, 4(4). Abstract.
Manfiolli AO, Mattos EC, de Assis LJ, Silva LP, Ulaş M, Brown NA, Silva-Rocha R, Bayram Ö, Goldman GH (2019). Aspergillus fumigatus High Osmolarity Glycerol Mitogen Activated Protein Kinases SakA and MpkC Physically Interact During Osmotic and Cell Wall Stresses. Frontiers in Microbiology, 10
Horta MAC, Thieme N, Gao Y, Burnum-Johnson KE, Nicora CD, Gritsenko MA, Lipton MS, Mohanraj K, de Assis LJ, Lin L, et al (2019). Broad Substrate-Specific Phosphorylation Events Are Associated with the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa. Frontiers in Microbiology, 10
Ribeiro LFC, Chelius C, Boppidi KR, Naik NS, Hossain S, Ramsey JJJ, Kumar J, Ribeiro LF, Ostermeier M, Tran B, et al (2019). Comprehensive Analysis of Aspergillus nidulans PKA Phosphorylome Identifies a Novel Mode of CreA Regulation. mBio, 10(2). Abstract.
Suriani Ribeiro M, Graciano de Paula R, Raquel Voltan A, de Castro RG, Carraro CB, José de Assis L, Stecca Steindorff A, Goldman GH, Silva RN, Ulhoa CJ, et al (2019). Endo-β-1,3-glucanase (GH16 Family) from Trichoderma harzianum Participates in Cell Wall Biogenesis but is Not Essential for Antagonism Against Plant Pathogens. Biomolecules, 9(12), 781-781. Abstract.  Full text.
Manfiolli AO, Siqueira FS, dos Reis TF, Van Dijck P, Schrevens S, Hoefgen S, Föge M, Straßburger M, de Assis LJ, Heinekamp T, et al (2019). Mitogen-Activated Protein Kinase Cross-Talk Interaction Modulates the Production of Melanins in Aspergillus fumigatus. mBio, 10(2). Abstract.
Ries LNA, Steenwyk JL, de Castro PA, de Lima PBA, Almeida F, de Assis LJ, Manfiolli AO, Takahashi-Nakaguchi A, Kusuya Y, Hagiwara D, et al (2019). Nutritional Heterogeneity Among Aspergillus fumigatus Strains Has Consequences for Virulence in a Strain- and Host-Dependent Manner. Frontiers in Microbiology, 10

2018

Manfiolli AO, dos Reis TF, de Assis LJ, de Castro PA, Silva LP, Hori JI, Walker LA, Munro CA, Rajendran R, Ramage G, et al (2018). Mitogen activated protein kinases (MAPK) and protein phosphatases are involved in Aspergillus fumigatus adhesion and biofilm formation. The Cell Surface, 1, 43-56.
de Assis LJ, Manfiolli A, Mattos E, Fabri JHTM, Malavazi I, Jacobsen ID, Brock M, Cramer RA, Thammahong A, Hagiwara D, et al (2018). Protein Kinase a and High-Osmolarity Glycerol Response Pathways Cooperatively Control Cell Wall Carbohydrate Mobilization in Aspergillus fumigatus. mBio, 9(6). Abstract.
de Assis LJ, Ulas M, Ries LNA, El Ramli NAM, Sarikaya-Bayram O, Braus GH, Bayram O, Goldman GH (2018). Regulation of. Aspergillus nidulans. CreA-Mediated Catabolite Repression by the F-Box Proteins Fbx23 and Fbx47. mBio, 9(3). Abstract.
Ries LNA, José de Assis L, Rodrigues FJS, Caldana C, Rocha MC, Malavazi I, Bayram Ö, Goldman GH (2018). The Aspergillus nidulans Pyruvate Dehydrogenase Kinases Are Essential to Integrate Carbon Source Metabolism. G3: Genes|Genomes|Genetics, 8(7), 2445-2463.

2017

Ries LNA, Rocha MC, de Castro PA, Silva-Rocha R, Silva RN, Freitas FZ, de Assis LJ, Bertolini MC, Malavazi I, Goldman GH, et al (2017). The Aspergillus fumigatus CrzA Transcription Factor Activates Chitin Synthase Gene Expression during the Caspofungin Paradoxical Effect. mBio, 8(3). Abstract.
dos Reis TF, Nitsche BM, de Lima PBA, de Assis LJ, Mellado L, Harris SD, Meyer V, dos Santos RAC, Riaño-Pachón DM, Ries LNA, et al (2017). The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans. Scientific Reports, 7(1).

2016

Bruder Nascimento ACMDO, dos Reis TF, de Castro PA, Hori JI, Bom VLP, de Assis LJ, Ramalho LNZ, Rocha MC, Malavazi I, Brown NA, et al (2016). Mitogen activated protein kinases SakAHOG1and MpkC collaborate forAspergillus fumigatusvirulence. Molecular Microbiology, 100(5), 841-859.

2015

de Assis LJ, Ries LNA, Savoldi M, dos Reis TF, Brown NA, Goldman GH (2015). Aspergillus nidulans protein kinase a plays an important role in cellulase production. Biotechnology for Biofuels, 8(1).
de Assis LJ, Ries LNA, Savoldi M, Dinamarco TM, Goldman GH, Brown NA (2015). Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism inAspergillus nidulans. G3: Genes|Genomes|Genetics, 5(5), 857-872.

2013

de Assis LJ, Zingali RB, Masuda CA, Rodrigues SP, Montero-Lomelí M (2013). Pyruvate decarboxylase activity is regulated by the Ser/Thr protein phosphatase Sit4p in the yeastSaccharomyces cerevisiae. FEMS Yeast Research, 13(6), 518-528.

2008

Masuda CA, Previato JO, Miranda MN, Assis LJ, Penha LL, Mendonça-Previato L, Montero-Lomelí MN (2008). Overexpression of the aldose reductaseGRE3 suppresses lithium-induced galactose toxicity inSaccharomyces cerevisiae. FEMS Yeast Research, 8(8), 1245-1253.

2007

Tomizawa MM, Dias ES, Assis LJD, Gomide PHO, Santos JBD (2007). Variabilidade genética de isolados do cogumelo Agaricus blazei por meio de marcadores RAPD. Ciência e Agrotecnologia, 31(4), 1242-1249. Abstract.

Leandro_Jose_de_Assis Details from cache as at 2020-07-15 00:36:35

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