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Dr Richard Tennant

Dr Richard Tennant

Research Fellow

 01392 72(3105)

 Biocatalysis Centre BC2.1

 

Biocatalysis Centre, University of Exeter, The Henry Wellcome Building for Biocatalysis, Stocker Road, Exeter, EX4 4QD , UK

Overview

I am a postdoctoral research fellow in the Exeter Microbial Biofuels Group (EMBG) for Professor John Love. During my Ph.D. in the EMBG, I investigated the phylogenetic relationships between living and fossil Botryococcus braunii algae. My roles in the EMBG involve being the principal operator of our flow cytometers and supporting with the imaging cytometer. Furthermore to cytometry, I perform both Illumina and Oxford Nanopore DNA sequencing for an array of different samples, ranging from palaeoenvironmental lake sediments, silage samples to more traditional bacterial cultures.

Research

Research interests

My research is focused on developing and engineering industrially relevant organisms for the production of biofuels. I have investigated the ancient genome of the alga Botryococcus braunii. Using a combination of flow cytometry and DNA sequencing I was able to extract sequenceable DNA from 8,500 year old colonies of B, braunii. and begin to explore the rationale for why the alga excretes hydrocarbons. Adjunct to the biofuels research I have been using Oxford Nanopore sequencing to study microbial communities in Holocene lake sediments, with a focus on how these species are linked to evidence for ancient agriculture.

Publications

Key publications | Publications by category | Publications by year

Key publications


Tennant RK, Ayine M, Power A, Gilman J, Hewlett M, James P, Singleton C, Parker D, Love J (2019). A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200. Microbiology Resource Announcements, 8(3). Full text.
Tennant RK, Holzer B, Love J, Tchilian E, White HN (2019). Higher levels of B-cell mutation in the early germinal centres of an inefficient secondary antibody response to a variant influenza haemagglutinin. Immunology, 157(1), 86-91. Abstract.  Author URL.  Full text.
Tennant RK, Lux T, Sambles C, Kuhn N, Petticrew E, Oldfield R, Parker D, Hatton J, Moore K, Lee R, et al (2019). Palaeogenomics of the Hydrocarbon Producing Microalga Botryococcus braunii. Scientific Reports, 9 Full text.
Gilman J, Singleton C, Tennant RK, James P, Howard TP, Lux T, Parker DA, Love J (2019). Rapid, Heuristic Discovery and Design of Promoter Collections in Non-Model Microbes for Industrial Applications. ACS Synth Biol, 8(5), 1175-1186. Abstract.  Author URL.  Full text.
Murray AJ, Love J, Redwood MD, Orozco RL, Tennant RK, Woodhall F, Goodridge F, Macaskie LE (2018). Enhancement of Photosynthetic Productivity by Quantum Dots Application. In Stavrou VN (Ed) Nonmagnetic and Magnetic Quantum Dots, IntechOpen. Full text.
Power A, Tennant RK, Jones RT, Tang Y, Du J, Worsley AT, Love J (2018). Monitoring Impacts of Urbanisation and Industrialisation on Air Quality in the Anthropocene Using Urban Pond Sediments. Frontiers in Earth Science, 6 Full text.

Publications by category


Journal articles

Tennant RK, Ayine M, Power A, Gilman J, Hewlett M, James P, Singleton C, Parker D, Love J (2019). A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200. Microbiology Resource Announcements, 8(3). Full text.
Tennant RK, Holzer B, Love J, Tchilian E, White HN (2019). Higher levels of B-cell mutation in the early germinal centres of an inefficient secondary antibody response to a variant influenza haemagglutinin. Immunology, 157(1), 86-91. Abstract.  Author URL.  Full text.
Tennant RK, Lux T, Sambles C, Kuhn N, Petticrew E, Oldfield R, Parker D, Hatton J, Moore K, Lee R, et al (2019). Palaeogenomics of the Hydrocarbon Producing Microalga Botryococcus braunii. Scientific Reports, 9 Full text.
Gilman J, Singleton C, Tennant RK, James P, Howard TP, Lux T, Parker DA, Love J (2019). Rapid, Heuristic Discovery and Design of Promoter Collections in Non-Model Microbes for Industrial Applications. ACS Synth Biol, 8(5), 1175-1186. Abstract.  Author URL.  Full text.
Power A, Tennant RK, Jones RT, Tang Y, Du J, Worsley AT, Love J (2018). Monitoring Impacts of Urbanisation and Industrialisation on Air Quality in the Anthropocene Using Urban Pond Sediments. Frontiers in Earth Science, 6 Full text.
Burton BR, Tennant RK, Love J, Titball RW, Wraith DC, White HN (2018). Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory. eLife Full text.
Tennant RK, Sambles CM, Diffey GE, Moore KA, Love J (2017). Metagenomic Analysis of Silage. Journal of Visualized Experiments, 119, e54936-e54936. Full text.
Tennant RK, Jones RT, Brock F, Cook C, Turney CSM, Love J, Lee R (2013). A new flow cytometry method enabling rapid purification of fossil pollen from terrestrial sediments for AMS radiocarbon dating. JOURNAL OF QUATERNARY SCIENCE, 28(3), 229-236. Author URL.
(2013). Flow sorting for fossil pollen. Nature, 497(7449), 291-291.
Tennant RK, Jones RT, Love J, Lee R (2012). A new flow cytometry method enabling rapid purification of diatoms from silica-rich lacustrine sediments. Journal of Paleolimnology, 49(2), 305-309. Full text.

Chapters

Murray AJ, Love J, Redwood MD, Orozco RL, Tennant RK, Woodhall F, Goodridge F, Macaskie LE (2018). Enhancement of Photosynthetic Productivity by Quantum Dots Application. In Stavrou VN (Ed) Nonmagnetic and Magnetic Quantum Dots, IntechOpen. Full text.
Cook C, Dayananda C, Tennant RK, Love J (2017). Third‐Generation Biofuels from the Microalga, Botryococcus braunii. In Love J, Bryant J (Eds.) Biofuels and Bioenergy, Wiley.

Publications by year


In Press

Tennant R, Rutten P (In Press). Calibration Protocol - Conversion of OD600 to Colony Forming Units (CFUs) v1 (protocols.io.zgnf3ve).
Tennant R, Rutten P (In Press). Calibration Protocol - Fluorescence Standard Curve with Fluorescein v1 (protocols.io.zgkf3uw).
Tennant R, Rutten P (In Press). Calibration Protocol - Particle Standard Curve with Microspheres v1 (protocols.io.zgjf3un).
Tennant R, Rutten P (In Press). Standard iGEM Cell Measurement Protocol v1 (protocols.io.zgmf3u6).
Beal J, Telmer C, Tennant R, Rutten P (In Press). iGEM Calibration Protocol - Flow Cytometry Cell Size v1 (protocols.io.2pegdje).
Beal J, Telmer C, Tennant R, Rutten P (In Press). iGEM Calibration Protocol - Flow Cytometry Fluorescence v1 (protocols.io.2pcgdiw).

2019

Tennant RK, Ayine M, Power A, Gilman J, Hewlett M, James P, Singleton C, Parker D, Love J (2019). A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200. Microbiology Resource Announcements, 8(3). Full text.
Tennant RK, Holzer B, Love J, Tchilian E, White HN (2019). Higher levels of B-cell mutation in the early germinal centres of an inefficient secondary antibody response to a variant influenza haemagglutinin. Immunology, 157(1), 86-91. Abstract.  Author URL.  Full text.
Tennant RK, Lux T, Sambles C, Kuhn N, Petticrew E, Oldfield R, Parker D, Hatton J, Moore K, Lee R, et al (2019). Palaeogenomics of the Hydrocarbon Producing Microalga Botryococcus braunii. Scientific Reports, 9 Full text.
Gilman J, Singleton C, Tennant RK, James P, Howard TP, Lux T, Parker DA, Love J (2019). Rapid, Heuristic Discovery and Design of Promoter Collections in Non-Model Microbes for Industrial Applications. ACS Synth Biol, 8(5), 1175-1186. Abstract.  Author URL.  Full text.

2018

Murray AJ, Love J, Redwood MD, Orozco RL, Tennant RK, Woodhall F, Goodridge F, Macaskie LE (2018). Enhancement of Photosynthetic Productivity by Quantum Dots Application. In Stavrou VN (Ed) Nonmagnetic and Magnetic Quantum Dots, IntechOpen. Full text.
Power A, Tennant RK, Jones RT, Tang Y, Du J, Worsley AT, Love J (2018). Monitoring Impacts of Urbanisation and Industrialisation on Air Quality in the Anthropocene Using Urban Pond Sediments. Frontiers in Earth Science, 6 Full text.
Burton BR, Tennant RK, Love J, Titball RW, Wraith DC, White HN (2018). Variant proteins stimulate more IgM+ GC B-cells revealing a mechanism of cross-reactive recognition by antibody memory. eLife Full text.

2017

Tennant RK, Sambles CM, Diffey GE, Moore KA, Love J (2017). Metagenomic Analysis of Silage. Journal of Visualized Experiments, 119, e54936-e54936. Full text.
Cook C, Dayananda C, Tennant RK, Love J (2017). Third‐Generation Biofuels from the Microalga, Botryococcus braunii. In Love J, Bryant J (Eds.) Biofuels and Bioenergy, Wiley.

2013

Tennant RK, Jones RT, Brock F, Cook C, Turney CSM, Love J, Lee R (2013). A new flow cytometry method enabling rapid purification of fossil pollen from terrestrial sediments for AMS radiocarbon dating. JOURNAL OF QUATERNARY SCIENCE, 28(3), 229-236. Author URL.
(2013). Flow sorting for fossil pollen. Nature, 497(7449), 291-291.

2012

Tennant RK, Jones RT, Love J, Lee R (2012). A new flow cytometry method enabling rapid purification of diatoms from silica-rich lacustrine sediments. Journal of Paleolimnology, 49(2), 305-309. Full text.

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Teaching

Supervision / Group

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