Publications by category
Journal articles
Li K, Ye Z, Chen G, Zheng K, Yin J, Debnath SC, Yan C, Hong Y, Guo Z, Wang P, et al (2023). Atopomonas sediminilitoris sp. nov. isolated from beach sediment of Zhairuo Island, China.
Antonie Van Leeuwenhoek,
116(2), 97-107.
Abstract:
Atopomonas sediminilitoris sp. nov. isolated from beach sediment of Zhairuo Island, China.
A novel bacterium designated A3.4T was isolated from the beach sediment of Zhairuo Island, which is located in the East China Sea. Strain A3.4T was found to be Gram-stain negative, cream coloured, rod-shaped, aerobic and motile via a single monopolar flagellum. The isolate grows at 20-37 °C (optimum 25-30 °C), at pH 6.0-8.0 (optimum pH 7.0-8.0), and in the presence of 0-5.0% (w/v) NaCl (optimum 0.5-1%). A3.4T has catalase and oxidase activity. The predominant fatty acids (≥ 10%) of the strain were identified as C16:0, summed feature 3 (C16:1 ω7c /C16:1 ω6c) and summed feature 8 (C18:1 ω7c /C18:1 ω6c). Q-9 was identified as the major isoprenoid quinone, with trace levels of Q-8 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The draft genome size is 3.55 Mb, with a DNA G + C content of 57.7 mol%. Analysis of the 16S rRNA gene sequence of strain A3.4T indicates that it belongs to the genus Atopomonas and shares high sequence similarity with Atopomonas hussainii JCM 19513T (97.60%). This classification was also supported by phylogenetic analysis using rpoB and several core genes. The genome of strain A3.4T shows an average nucleotide identity of 82.3%, an amino acid identity of 83.0%, and a digital DNA-DNA hybridization value of 22.1% with A. hussainii. In addition, 20 conserved signature indels (CSIs) were identified to be specific for A3.4T and A. hussainii, demonstrating that the strain A3.4T is closely related to A. hussainii rather than other species of family Pseudomonadaceae. Hundreds of unique genes were identified in the genomes of A3.4T and A. hussainii, which may underly multiple phenotypic differences between these strains. Based on phenotypic, chemotaxonomic, phylogenetic, and genomic investigations, strain A3.4T is concluded to represent a novel species of the genus Atopomonas, for which the name Atopomonas sediminilitoris sp. nov. is proposed. The type strain is A3.4T (= LMG 32563T = MCCC 1K07166T).
Abstract.
Author URL.
Debnath SC, McMurtrie J, Temperton B, Delamare-Deboutteville J, Mohan CV, Tyler CR (2023). Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease.
Aquaculture InternationalAbstract:
Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease
AbstractAquaculture is playing an increasingly important role in global food security, especially for low-income and food-deficit countries. The majority of aquaculture production occurs in freshwater earthen ponds and tilapia has quickly become one of the most widely adopted culture species in these systems. Tilapia are now farmed in over 140 countries facilitated by their ease of production, adaptability to a wide range of environmental conditions, fast growth, and high nutritional value. Typically, tilapia have been considered a hardy, disease resilient species; however, the disease is increasing with subsequent threats to the industry as their production is intensified. In this review, we discuss tilapia production, with a focus on Bangladesh as one of the top producing countries, and highlight the problems associated with disease and treatment approaches for them, including the misuse of antimicrobials. We address a key missing component in understanding health and disease processes for sustainable production in aquaculture, specifically the role played by the microbiome. Here we examine the importance of the microbiome in supporting health, focused on the symbiotic microbial community of the fish skin mucosal surface, the abiotic and biotic factors that influence the microbiome, and the shifts that are associated with diseased states. We also identify conserved taxa of skin microbiomes that may be used as indicators of health status for tilapia offering new opportunities to mitigate and manage the disease and optimize environmental growing conditions and farming practices.
Abstract.
Zheng K, Hong Y, Guo Z, Debnath SC, Yan C, Li K, Chen G, Xu J, Wu F, Zheng D, et al (2022). Acinetobacter sedimenti sp. nov. isolated from beach sediment.
International Journal of Systematic and Evolutionary Microbiology,
72(11).
Abstract:
Acinetobacter sedimenti sp. nov. isolated from beach sediment
A Gram-stain-negative, aerobic, non-motile, non-haemolytic, oxidase-negative, catalase-positive bacillus strain (A3.8T) was isolated from beach sediment from Zhairuo Island, PR China. The strain grew at pH 6.0–9.0 (optimum, 7.0), with 0–4.5 % NaCl (optimum, 2 %) and at 10–35 °C (optimum, 30 °C). Its whole-genome sequence was 2.5 Mb in size, with a DNA G+C content of 41.6 mol%. On the basis of the results of core genome phylogenetic analysis, A3.8T represents a separate branch within the clade formed by five species of the genus Acinetobacter with ‘Acinetobacter marinus’ as the most closely related species. The average nucleotide identity compared with the closely related species of the genus Acinetobacter was below 83.66 % and digital DNA–DNA hybridization values were less than 28.80 %. The predominant fatty acids included C18: 1 ω9c, C16: 0 and summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c). Q-9 was the major respiratory quinone. The polar lipids are mainly composed of diphosphatidyl-glycerol, phosphatidylglycerol, phosphatidylethanolamine, two phospholipids, an aminolipid and four unknown lipids. A3.8T cannot assimilate dl-lactate and weakly utilizes l-glutamate, l-leucine, l-phenylalanine and l-tartrate, which distinguishes it from other species of the genus Acinetobacter. On the basis of the genotype, phenotype and biochemical data, strain A3.8T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter sedimenti sp. nov. is proposed. The type strain is A3.8T (=MCCC 1K07161T=LMG 32568T).
Abstract.
Wang P, Sheng H, Zheng K, Hong Y, Debnath SC, Yan C, Li K, Chen G, Xu J, Wu F, et al (2022). Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
Arch Microbiol,
204(7).
Abstract:
Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
A Gram-negative, aerobic, non-motile, oxidase-positive, catalase-positive, methyl red-positive, and lipase-negative bacterium, designated A5.8T, was isolated from beach sediment of Zhairuo Island located in the East China Sea. Growth occurred at 10-40 °C (optimum, 30 °C), pH 5.5-9.5 (optimum, 7.5), and 0-2% NaCl (optimum, 1.5%). Based on 16S rRNA gene sequence analysis, strain A5.8T belongs to the genus Ancylobacter, sharing the highest similarity with Ancylobacter aquaticus JCM 20518T (98.0%). Its polar lipids mainly consist of phosphatidylethanolamine (PE) and phosphatidylcholine (PC). The predominant fatty acids are summed feature 8 (C18:1ω7c and/or C18:1ω6c, 91.0%), and the major respiratory quinone is Q-10. The DNA G + C content is 67.2 mol%. Based on above analysis, as well as digital DNA-DNA hybridization (22.5-22.9%) and average nucleotide identity (83.0-83.6%) of strain A5.8T with reference type strains of the genus Ancylobacter, strain A5.8T was suggested to represent a novel species of the genus Ancylobacter, for which the name Ancylobacter gelatini sp. nov. is proposed. The type strain is A5.8T (= MCCC 1K07167T = LMG 32566T).
Abstract.
Author URL.
Sharifuzzaman SM, Rasid MH, Rubby IA, Debnath SC, Xing B, Chen G, Chowdhury MSN, Hossain MS (2021). DNA barcoding confirms a new record of flyingfish Cheilopogon spilonotopterus (Beloniformes: Exocoetidae) from the northern Bay of Bengal. Conservation Genetics Resources, 13(3), 323-328.
Khan I, Debnath SC, Yan C, Chen C, Xu Y, Wang W-J, Yu Y-C, Zheng D-Q, Xu J-Z, Wang P-M, et al (2020). Flavobacterium ajazii sp. nov. Isolated from Seaweed of Gouqi Island, China. Current Microbiology, 77(10), 2925-2932.
Debnath SC, Chen C, Khan I, Wang W-J, Zheng D-Q, Xu J-Z, Wang P-M (2020). Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
International Journal of Systematic and Evolutionary Microbiology,
70(7), 4250-4260.
Abstract:
Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12T) and sediment of Qinghai Lake (strain LaA7.5T), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus
.
. Flavobacterium
.
. showing the highest 16S rRNA sequence similarities to
.
. Flavobacterium arcticum
.
. SM1502T(97.6–97.7 %) and
.
. Flavobacterium suzhouense
.
. XIN-1T(96.5–96.6 %). Moreover, strains SaA2.12T and LaA7.5T showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C15 : 0, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12T and LaA7.5T showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12T and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5T and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12T and LaA7.5T were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12T represents a novel species with the name Flavobacterium salilacus sp. nov. with the type strain SaA2.12T (=KCTC 72220T=MCCC 1K03618T) and strain LaA7.5T as a subspecies within novel Flavobacterium salilacus with the name Flavobacterium salilacus subsp. altitudinum subsp. nov. with the type strain LaA7.5T (=KCTC 72806T=MCCC 1K04372T). These propositions automatically create Flavobacterium salilacus subsp. salilacus subsp. nov. with SaA2.12T (=KCTC 72220T=MCCC 1K03618T) as the type strain.
Abstract.
Khan I, Xie W-L, Yu Y-C, Sheng H, Xu Y, Wang J-Q, Debnath SC, Xu J-Z, Zheng D-Q, Ding W-J, et al (2020). Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes.
Marine Drugs,
18(12), 652-652.
Abstract:
Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A.
Abstract.
Debnath SC, Chen C, Liu S-X, Di Y-N, Zheng D-Q, Li X-Y, Xu X-W, Xu J-Z, Wang P-M (2019). Flavobacterium sharifuzzamanii sp. nov. Isolated from the Sediments of the East China Sea. Current Microbiology, 76(3), 297-303.
Debnath SC, Miyah AMA, Chen C, Sheng H, Xu X-W, Wu Y-H, Zheng D-Q, Xu J-Z, Di Y-N, Wang P-M, et al (2019). Flavobacterium zhairuonensis sp. nov. a gliding bacterium isolated from marine sediment of the East China Sea. Journal of Microbiology, 57(12), 1065-1072.
Chen C, Ye Y, Wang R, Zhang Y, Wu C, Debnath SC, Ma Z, Wang J, Wu M (2018). Streptomyces nigra sp. nov. Is a Novel Actinobacterium Isolated from Mangrove Soil and Exerts a Potent Antitumor Activity in Vitro. Frontiers in Microbiology, 9
Publications by year
2023
Li K, Ye Z, Chen G, Zheng K, Yin J, Debnath SC, Yan C, Hong Y, Guo Z, Wang P, et al (2023). Atopomonas sediminilitoris sp. nov. isolated from beach sediment of Zhairuo Island, China.
Antonie Van Leeuwenhoek,
116(2), 97-107.
Abstract:
Atopomonas sediminilitoris sp. nov. isolated from beach sediment of Zhairuo Island, China.
A novel bacterium designated A3.4T was isolated from the beach sediment of Zhairuo Island, which is located in the East China Sea. Strain A3.4T was found to be Gram-stain negative, cream coloured, rod-shaped, aerobic and motile via a single monopolar flagellum. The isolate grows at 20-37 °C (optimum 25-30 °C), at pH 6.0-8.0 (optimum pH 7.0-8.0), and in the presence of 0-5.0% (w/v) NaCl (optimum 0.5-1%). A3.4T has catalase and oxidase activity. The predominant fatty acids (≥ 10%) of the strain were identified as C16:0, summed feature 3 (C16:1 ω7c /C16:1 ω6c) and summed feature 8 (C18:1 ω7c /C18:1 ω6c). Q-9 was identified as the major isoprenoid quinone, with trace levels of Q-8 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The draft genome size is 3.55 Mb, with a DNA G + C content of 57.7 mol%. Analysis of the 16S rRNA gene sequence of strain A3.4T indicates that it belongs to the genus Atopomonas and shares high sequence similarity with Atopomonas hussainii JCM 19513T (97.60%). This classification was also supported by phylogenetic analysis using rpoB and several core genes. The genome of strain A3.4T shows an average nucleotide identity of 82.3%, an amino acid identity of 83.0%, and a digital DNA-DNA hybridization value of 22.1% with A. hussainii. In addition, 20 conserved signature indels (CSIs) were identified to be specific for A3.4T and A. hussainii, demonstrating that the strain A3.4T is closely related to A. hussainii rather than other species of family Pseudomonadaceae. Hundreds of unique genes were identified in the genomes of A3.4T and A. hussainii, which may underly multiple phenotypic differences between these strains. Based on phenotypic, chemotaxonomic, phylogenetic, and genomic investigations, strain A3.4T is concluded to represent a novel species of the genus Atopomonas, for which the name Atopomonas sediminilitoris sp. nov. is proposed. The type strain is A3.4T (= LMG 32563T = MCCC 1K07166T).
Abstract.
Author URL.
Debnath SC, McMurtrie J, Temperton B, Delamare-Deboutteville J, Mohan CV, Tyler CR (2023). Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease.
Aquaculture InternationalAbstract:
Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease
AbstractAquaculture is playing an increasingly important role in global food security, especially for low-income and food-deficit countries. The majority of aquaculture production occurs in freshwater earthen ponds and tilapia has quickly become one of the most widely adopted culture species in these systems. Tilapia are now farmed in over 140 countries facilitated by their ease of production, adaptability to a wide range of environmental conditions, fast growth, and high nutritional value. Typically, tilapia have been considered a hardy, disease resilient species; however, the disease is increasing with subsequent threats to the industry as their production is intensified. In this review, we discuss tilapia production, with a focus on Bangladesh as one of the top producing countries, and highlight the problems associated with disease and treatment approaches for them, including the misuse of antimicrobials. We address a key missing component in understanding health and disease processes for sustainable production in aquaculture, specifically the role played by the microbiome. Here we examine the importance of the microbiome in supporting health, focused on the symbiotic microbial community of the fish skin mucosal surface, the abiotic and biotic factors that influence the microbiome, and the shifts that are associated with diseased states. We also identify conserved taxa of skin microbiomes that may be used as indicators of health status for tilapia offering new opportunities to mitigate and manage the disease and optimize environmental growing conditions and farming practices.
Abstract.
2022
Zheng K, Hong Y, Guo Z, Debnath SC, Yan C, Li K, Chen G, Xu J, Wu F, Zheng D, et al (2022). Acinetobacter sedimenti sp. nov. isolated from beach sediment.
International Journal of Systematic and Evolutionary Microbiology,
72(11).
Abstract:
Acinetobacter sedimenti sp. nov. isolated from beach sediment
A Gram-stain-negative, aerobic, non-motile, non-haemolytic, oxidase-negative, catalase-positive bacillus strain (A3.8T) was isolated from beach sediment from Zhairuo Island, PR China. The strain grew at pH 6.0–9.0 (optimum, 7.0), with 0–4.5 % NaCl (optimum, 2 %) and at 10–35 °C (optimum, 30 °C). Its whole-genome sequence was 2.5 Mb in size, with a DNA G+C content of 41.6 mol%. On the basis of the results of core genome phylogenetic analysis, A3.8T represents a separate branch within the clade formed by five species of the genus Acinetobacter with ‘Acinetobacter marinus’ as the most closely related species. The average nucleotide identity compared with the closely related species of the genus Acinetobacter was below 83.66 % and digital DNA–DNA hybridization values were less than 28.80 %. The predominant fatty acids included C18: 1 ω9c, C16: 0 and summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c). Q-9 was the major respiratory quinone. The polar lipids are mainly composed of diphosphatidyl-glycerol, phosphatidylglycerol, phosphatidylethanolamine, two phospholipids, an aminolipid and four unknown lipids. A3.8T cannot assimilate dl-lactate and weakly utilizes l-glutamate, l-leucine, l-phenylalanine and l-tartrate, which distinguishes it from other species of the genus Acinetobacter. On the basis of the genotype, phenotype and biochemical data, strain A3.8T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter sedimenti sp. nov. is proposed. The type strain is A3.8T (=MCCC 1K07161T=LMG 32568T).
Abstract.
Wang P, Sheng H, Zheng K, Hong Y, Debnath SC, Yan C, Li K, Chen G, Xu J, Wu F, et al (2022). Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
Arch Microbiol,
204(7).
Abstract:
Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
A Gram-negative, aerobic, non-motile, oxidase-positive, catalase-positive, methyl red-positive, and lipase-negative bacterium, designated A5.8T, was isolated from beach sediment of Zhairuo Island located in the East China Sea. Growth occurred at 10-40 °C (optimum, 30 °C), pH 5.5-9.5 (optimum, 7.5), and 0-2% NaCl (optimum, 1.5%). Based on 16S rRNA gene sequence analysis, strain A5.8T belongs to the genus Ancylobacter, sharing the highest similarity with Ancylobacter aquaticus JCM 20518T (98.0%). Its polar lipids mainly consist of phosphatidylethanolamine (PE) and phosphatidylcholine (PC). The predominant fatty acids are summed feature 8 (C18:1ω7c and/or C18:1ω6c, 91.0%), and the major respiratory quinone is Q-10. The DNA G + C content is 67.2 mol%. Based on above analysis, as well as digital DNA-DNA hybridization (22.5-22.9%) and average nucleotide identity (83.0-83.6%) of strain A5.8T with reference type strains of the genus Ancylobacter, strain A5.8T was suggested to represent a novel species of the genus Ancylobacter, for which the name Ancylobacter gelatini sp. nov. is proposed. The type strain is A5.8T (= MCCC 1K07167T = LMG 32566T).
Abstract.
Author URL.
2021
Sharifuzzaman SM, Rasid MH, Rubby IA, Debnath SC, Xing B, Chen G, Chowdhury MSN, Hossain MS (2021). DNA barcoding confirms a new record of flyingfish Cheilopogon spilonotopterus (Beloniformes: Exocoetidae) from the northern Bay of Bengal. Conservation Genetics Resources, 13(3), 323-328.
2020
Khan I, Debnath SC, Yan C, Chen C, Xu Y, Wang W-J, Yu Y-C, Zheng D-Q, Xu J-Z, Wang P-M, et al (2020). Flavobacterium ajazii sp. nov. Isolated from Seaweed of Gouqi Island, China. Current Microbiology, 77(10), 2925-2932.
Debnath SC, Chen C, Khan I, Wang W-J, Zheng D-Q, Xu J-Z, Wang P-M (2020). Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
International Journal of Systematic and Evolutionary Microbiology,
70(7), 4250-4260.
Abstract:
Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12T) and sediment of Qinghai Lake (strain LaA7.5T), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus
.
. Flavobacterium
.
. showing the highest 16S rRNA sequence similarities to
.
. Flavobacterium arcticum
.
. SM1502T(97.6–97.7 %) and
.
. Flavobacterium suzhouense
.
. XIN-1T(96.5–96.6 %). Moreover, strains SaA2.12T and LaA7.5T showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C15 : 0, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12T and LaA7.5T showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12T and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5T and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12T and LaA7.5T were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12T represents a novel species with the name Flavobacterium salilacus sp. nov. with the type strain SaA2.12T (=KCTC 72220T=MCCC 1K03618T) and strain LaA7.5T as a subspecies within novel Flavobacterium salilacus with the name Flavobacterium salilacus subsp. altitudinum subsp. nov. with the type strain LaA7.5T (=KCTC 72806T=MCCC 1K04372T). These propositions automatically create Flavobacterium salilacus subsp. salilacus subsp. nov. with SaA2.12T (=KCTC 72220T=MCCC 1K03618T) as the type strain.
Abstract.
Khan I, Xie W-L, Yu Y-C, Sheng H, Xu Y, Wang J-Q, Debnath SC, Xu J-Z, Zheng D-Q, Ding W-J, et al (2020). Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes.
Marine Drugs,
18(12), 652-652.
Abstract:
Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A.
Abstract.
2019
Debnath SC, Chen C, Liu S-X, Di Y-N, Zheng D-Q, Li X-Y, Xu X-W, Xu J-Z, Wang P-M (2019). Flavobacterium sharifuzzamanii sp. nov. Isolated from the Sediments of the East China Sea. Current Microbiology, 76(3), 297-303.
Debnath SC, Miyah AMA, Chen C, Sheng H, Xu X-W, Wu Y-H, Zheng D-Q, Xu J-Z, Di Y-N, Wang P-M, et al (2019). Flavobacterium zhairuonensis sp. nov. a gliding bacterium isolated from marine sediment of the East China Sea. Journal of Microbiology, 57(12), 1065-1072.
2018
Chen C, Ye Y, Wang R, Zhang Y, Wu C, Debnath SC, Ma Z, Wang J, Wu M (2018). Streptomyces nigra sp. nov. Is a Novel Actinobacterium Isolated from Mangrove Soil and Exerts a Potent Antitumor Activity in Vitro. Frontiers in Microbiology, 9
