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Dr Steffen Scholpp

Dr Steffen Scholpp

Associate Professor, Cell and Developmental Biology

 7451

 +44 (0)1392 727451

 Living Systems Institute T02.12

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

Tissue development is a key process for life starting from the earliest embryonic stages during which cells differentiate into later organs composing an entire body. An essential component for these developmental processes but also for tissue regeneration and stem cell regulation is the communication of cells by chemical signalling. The highly conserved family of Wnt proteins represents important regulators of cell behaviour, tissue development and homeostasis by inducing responses in a concentration-dependent manner. We identified a novel way of spreading Wnt proteins in vertebrates: Wnt molecules are mobilized on specific cell protrusions known as cytonemes. These specialized signalling filopodia transmit signal proteins between communicating cells and allow a high degree of control of propagation speed, direction and concentration of the transmitted ligand. The signalling molecules are delivered directly to the receiving cells by a direct-contact model and parameters such as cytoneme length or speed of filopodia formation dictate local Wnt concentration.

In our research, we use the zebrafish embryo to investigate how intercellular Wnt protein transport is regulated and how signals are subsequently delivered to the target cell in a living vertebrate organism. By understanding Wnt dissemination mechanisms, we will be able to control Wnt signalling in development, and regeneration. This research is funded by the Biotechnology and Biological Sciences Research Council (BBSRC).

We further use gastric cancer cells to investigate how cytonemes mobilize Wnts in tumour tissue. Our research will open up new strategies to control Wnt signalling by altering its transport route in the tumour microenvironment. Our research is supported by the Medical Research Council (MRC).

At the Living Systems Institute, we collaborate with biophysicists using super-resolution microscopy to describe these signalling processes in a quantitative way on a molecular level. As it is very difficult to determine the specific impact of individual parameters in a complex biological system by a purely experimental approach, we interact with mathematicians using computational modelling. Together, we develop a robust mathematical model for the distribution of signal molecules on the basis of signalling filopodia. Due to the conserved nature of vertebrate cell behaviour, our results will be relevant to Wnt signalling during human embryonic development and could suggest novel vulnerabilities to Wnt-dependent diseases – a prerequisite for the development of novel therapeutics.

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Qualifications

2009-2016 Emmy-Noether Research Group Leader (Assistant Professor)

2003 PhD Neurobiology (Hons., summa cum laude), University of Heidelberg, Germany

Career

2017-present Associate Professor of Cell and Developmental Biology, Biosciences, University of Exeter, UK

2009-2016 Emmy-Noether group leader (Assistant Professor) at the Karlsruhe Institute of Technology (KIT), Germany

2004-2009 Postdoctoral Research Fellow with Prof Andrew Lumsden, FRS, MRC Centre for Developmental Neurobiology, King’s College London, UK

2003-2004 Postdoctoral Research Associate with Prof Michael Brand, Max Planck-Institute of Cell Biology and Genetics (MPI-CBG), Dresden, Germany

2003 PhD Neurobiology (Hons., summa cum laude), University of Heidelberg, Germany

1999-2003 PhD student in Neurobiology (Laboratory of Prof Michael Brand), University of Heidelberg, Germany and Max Planck-Institute of Cell Biology and Genetics (MPI-CBG), Dresden, Germany

Links

Wnt protein (red) on cytoneme tips in vivo

Research

Research interests

After secretion, developmental signals known as morphogens must travel relatively long distances to form a concentration gradient that the responding tissue uses to acquire positional information. The role of morphogen transport and endocytic trafficking in this process is the subject of intense debate. Wnt proteins regulate developmental processes, tissue regeneration and stem cell maintenance. It has been postulated that Wnt/β-catenin signalling forms concentration gradients across responsive tissues and act as morphogens. However, little is known about the transport mechanism for these lipid-modified signalling proteins in vertebrates.

Recently, we showed that Wnt8a is transported on short, actin-based filopodia known as cytonemes to contact responding cells and activate signalling during neural plate formation in zebrafish (1).Wnt/ Ror2 signalling regulates the formation of these Wnt cytonemes (5). Enhanced formation of cytonemes increases the effective signalling range of Wnt by facilitating spreading. Consistently, reduction in cytonemes leads to a restricted distribution of the ligand and a limited signalling range. Using a numerical simulation, we provide evidence that such a short-range transport system for Wnt has a long-range signalling function.

We also showed that Wnt cytonemes are essential to disseminate Wnt signalling in gastric tumours. In this tissue, fibroblasts produce Wnts and distribute them via a dynamic cytoneme network within the cancer tissue to regulate proliferation and metastasis.

After contact by Wnt/β-catenin positive filopodia, a multi-protein complex at the plasma membrane assembles clustering membrane-bound receptors and intracellular signal transducers into the so-called Lrp6-signalosome. Our imaging studies in live zebrafish embryos showed that the signalosome is a highly dynamic structure, which is continuously assembled and disassembled by a Dvl2-mediated endocytic process (2). We showed that this endocytic process is not only essential for ligand-receptor internalization but also for signalling.

We conclude that a cytoneme-based transport system for Wnt and subsequent endocytosis is important for Wnt/β-catenin signalling in development and disease (3,4,6).

(1) Stanganello et al., Nature Comms., 2015; (2) Hagemann, et al., J.Cell Sci., 2014; (3) Zhang and Scholpp, 2019; (4) Brunt and Scholpp, CMLS, 2017; (5) Mattes et al., eLife, 2018. (6) Brunt et al., Nat Comms 2021

Research projects

OPEN POSITIONS:

We're recruiting talented graduates from all areas of bioscience to become MSc students, PhD students and postdoctoral researchers in our lab. Contact us to talk about possibilities. 

Join our team!

Research grants

  • 2020 BBSRC
    Lattice-based Microscopy to analyse subcellular dynamics in living specimen;: BBSRC 19-ALERT Mid-range equipment initiative; 2020-2021; BB/T017899/1
  • 2019 Medical Research Council
    Deciphering the molecular mechanism of Wnt trafficking in gastric cancer; MRC Research Grant; 2019-2022; MR/S007970/1
  • 2019 BBSRC
    Quantitative analysis of cytoneme-based Wnt trafficking and signalling in vivo; BBSRC Responsive Mode; 2019-2022; BB/S016295/1
  • 2019 BBSRC
    HPF to enable a high-quality ultrastructural analysis of biological samples; BBSRC 18-ALERT Mid-range equipment initative; 2019-2020; BB/R019499/1
  • 2018 BBSRC
    A Single Molecule Detection Unit to perform in vivo FCS and FLIM-FRET analysis in zebrafish; BBSRC 17-ALERT Mid-range equipment initiative; 2018-2019; BB/R013764/1
  • 2017 Wellcome Trust
    CBMA Seed Corn Award, 2018; WT105618MA

Publications

Key publications | Publications by category | Publications by year

Key publications


Liu T-L, Upadhyayula S, Milkie D, Singh V, Wang K, Swineburn I, Scholpp S, Megason S, Kirchhausen T, Betzig E, et al (In Press). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science
Winter MJ, Ono Y, Ball JS, Walentinsson A, Michaelsson E, Tochwin A, Scholpp S, Tyler CR, Rees S, Hetheridge MJ, et al (2022). A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation. Frontiers in Pharmacology, 13
Brunt L, Greicius G, Rogers S, Evans BD, Virshup DM, Wedgwood KCA, Scholpp S (2021). Vangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling. Nat Commun, 12(1). Abstract.  Author URL.
Bosze B, Ono Y, Mattes B, Sinner C, Gourain V, Thumberger T, Tlili S, Wittbrodt J, Saunders TE, Strähle U, et al (2020). Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish. Histochemistry and Cell Biology, 154(5), 463-480. Abstract.
Zhang C, Scholpp S (2019). Cytonemes in development. Current Opinion in Genetics and Development, 57, 25-30. Abstract.
Routledge D, Scholpp S (2019). Mechanisms of intercellular Wnt transport. Development, 146(10). Abstract.  Author URL.

Publications by category


Journal articles

Gardilla AC, Sanchez D, Brunt L, Scholpp S (In Press). From top to bottom: Cell polarity in Hedgehog and Wnt trafficking. BMC Biology
Liu T-L, Upadhyayula S, Milkie D, Singh V, Wang K, Swineburn I, Scholpp S, Megason S, Kirchhausen T, Betzig E, et al (In Press). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science
Brunt L, Greicius G, Evans BD, Virshup DM, Wedgwood KCA, Scholpp S (In Press). Vangl2 regulates the dynamics of Wnt cytonemes in vertebrates.  Abstract.
Winter MJ, Ono Y, Ball JS, Walentinsson A, Michaelsson E, Tochwin A, Scholpp S, Tyler CR, Rees S, Hetheridge MJ, et al (2022). A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation. Frontiers in Pharmacology, 13
Routledge D, Rogers S, Ono Y, Brunt L, Meniel V, Tornillo G, Ashktorab H, Phesse TJ, Scholpp S (2022). The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer. eLife, 11 Abstract.
Rogers S, Scholpp S (2022). Vertebrate Wnt5a – at the crossroads of cellular signalling. Seminars in Cell & Developmental Biology, 125, 3-10.
Sutton G, Kelsh RN, Scholpp S (2021). Review: the Role of Wnt/β-Catenin Signalling in Neural Crest Development in Zebrafish. Frontiers in Cell and Developmental Biology, 9 Abstract.
Brunt L, Greicius G, Rogers S, Evans BD, Virshup DM, Wedgwood KCA, Scholpp S (2021). Vangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling. Nat Commun, 12(1). Abstract.  Author URL.
Cavallo JC, Scholpp S, Flegg MB (2020). Delay-driven oscillations via Axin2 feedback in the Wnt/β-catenin signalling pathway. J Theor Biol, 507 Abstract.  Author URL.
Alshami IJJ, Ono Y, Correia A, Hacker C, Lange A, Scholpp S, Kawasaki M, Ingham PW, Kudoh T (2020). Development of the electric organ in embryos and larvae of the knifefish, Brachyhypopomus gauderio. Developmental Biology, 466(1-2), 99-108.
Scholpp S (2020). Introduction: in vivo cell biology in zebrafish. Histochem Cell Biol, 154(5), 457-461. Author URL.
Rosenbauer J, Zhang C, Mattes B, Reinartz I, Wedgwood K, Schindler S, Sinner C, Scholpp S, Schug A (2020). Modeling of Wnt-mediated tissue patterning in vertebrate embryogenesis. PLOS Computational Biology, 16(6), e1007417-e1007417.
Bosze B, Ono Y, Mattes B, Sinner C, Gourain V, Thumberger T, Tlili S, Wittbrodt J, Saunders TE, Strähle U, et al (2020). Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish. Histochemistry and Cell Biology, 154(5), 463-480. Abstract.
Dawes ML, Soeller C, Scholpp S (2020). Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo. HISTOCHEMISTRY AND CELL BIOLOGY, 154(5), 507-519. Author URL.
Zhang C, Scholpp S (2019). Cytonemes in development. Current Opinion in Genetics and Development, 57, 25-30. Abstract.
Routledge D, Scholpp S (2019). Mechanisms of intercellular Wnt transport. Development, 146(10). Abstract.  Author URL.
Mattes B, Scholpp S (2018). Emerging role of contact-mediated cell communication in tissue development and diseases. Histochemistry and Cell Biology, 150, 431-442. Abstract.
Brunt L, Scholpp S (2018). The function of endocytosis in Wnt signaling. Cellular and Molecular Life Sciences, 75(5), 785-795. Abstract.
Scholpp S (2018). Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates. eLife
Stanganello E, Scholpp S (2016). Role of cytonemes in Wnt transport. J Cell Sci, 129(4), 665-672. Abstract.  Author URL.
Brinkmann E-M, Mattes B, Kumar R, Hagemann AIH, Gradl D, Scholpp S, Steinbeisser H, Kaufmann LT, Özbek S (2016). Secreted Frizzled-related Protein 2 (sFRP2) Redirects Non-canonical Wnt Signaling from Fz7 to Ror2 during Vertebrate Gastrulation. J Biol Chem, 291(26), 13730-13742. Abstract.  Author URL.
Stanganello E, Hagemann AIH, Mattes B, Sinner C, Meyen D, Weber S, Schug A, Raz E, Scholpp S (2015). Filopodia-based Wnt transport during vertebrate tissue patterning. Nat Commun, 6 Abstract.  Author URL.
Hirschbiel AF, Geyer S, Yameen B, Welle A, Nikolov P, Giselbrecht S, Scholpp S, Delaittre G, Barner-Kowollik C (2015). Photolithographic patterning of 3D-formed polycarbonate films for targeted cell guiding. Adv Mater, 27(16), 2621-2626. Abstract.  Author URL.
Rengarajan C, Matzke A, Reiner L, Orian-Rousseau V, Scholpp S (2014). Endocytosis of Fgf8 is a double-stage process and regulates spreading and signaling. PLoS One, 9(1). Abstract.  Author URL.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-Catenin signaling complex in zebrafish embryos. Development, 141(19), e1907-e1907.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-catenin signaling complex in zebrafish embryos. J Cell Sci, 127(Pt 18), 3970-3982. Abstract.  Author URL.
Chatterjee M, Guo Q, Weber S, Scholpp S, Li JY (2014). Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shh in the diencephalon in vertebrates. BMC Biol, 12 Abstract.  Author URL.
Chen Q, Su Y, Wesslowski J, Hagemann AI, Ramialison M, Wittbrodt J, Scholpp S, Davidson G (2014). Tyrosine phosphorylation of LRP6 by Src and Fer inhibits Wnt/β-catenin signalling. EMBO Rep, 15(12), 1254-1267. Abstract.  Author URL.
Efremov AN, Stanganello E, Welle A, Scholpp S, Levkin PA (2013). Micropatterned superhydrophobic structures for the simultaneous culture of multiple cell types and the study of cell-cell communication. Biomaterials, 34(7), 1757-1763. Abstract.  Author URL.
Schmidt R, Strähle U, Scholpp S (2013). Neurogenesis in zebrafish - from embryo to adult. Neural Dev, 8 Abstract.  Author URL.
Hagemann AIH, Scholpp S (2012). The Tale of the Three Brothers - Shh, Wnt, and Fgf during Development of the Thalamus. Front Neurosci, 6 Abstract.  Author URL.
Mattes B, Weber S, Peres J, Chen Q, Davidson G, Houart C, Scholpp S (2012). Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Dev, 7 Abstract.  Author URL.
Peukert D, Weber S, Lumsden A, Scholpp S (2011). Lhx2 and Lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating Wnt signaling. PLoS Biol, 9(12). Abstract.  Author URL.
Scholpp S, Lumsden A (2010). Building a bridal chamber: development of the thalamus. Trends Neurosci, 33(8), 373-380. Abstract.  Author URL.
Peukert D, Scholpp S (2010). Ontogenesis of the brain: the development of the thalamus - the gateway to consciousness. BioSpektrum, 16(6), 639-643. Abstract.
Fassier C, Hutt JA, Scholpp S, Lumsden A, Giros B, Nothias F, Schneider-Maunoury S, Houart C, Hazan J (2010). Zebrafish atlastin controls motility and spinal motor axon architecture via inhibition of the BMP pathway. Nat Neurosci, 13(11), 1380-1387. Abstract.  Author URL.
Yu SR, Burkhardt M, Nowak M, Ries J, Petrásek Z, Scholpp S, Schwille P, Brand M (2009). Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules. Nature, 461(7263), 533-536. Abstract.  Author URL.
Scholpp S, Delogu A, Gilthorpe J, Peukert D, Schindler S, Lumsden A (2009). Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus. Proc Natl Acad Sci U S A, 106(47), 19895-19900. Abstract.  Author URL.
Wendl T, Adzic D, Schoenebeck JJ, Scholpp S, Brand M, Yelon D, Rohr KB (2007). Early developmental specification of the thyroid gland depends on han-expressing surrounding tissue and on FGF signals. Development, 134(15), 2871-2879. Abstract.  Author URL.
Scholpp S (2007). Hedgehogs, Fluorescence Imaging & Brain Development. infocus Magazine, 66-75.
Scholpp S, Foucher I, Staudt N, Peukert D, Lumsden A, Houart C (2007). Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development, 134(17), 3167-3176. Abstract.  Author URL.
Erickson T, Scholpp S, Brand M, Moens CB, Waskiewicz AJ (2007). Pbx proteins cooperate with Engrailed to pattern the midbrain-hindbrain and diencephalic-mesencephalic boundaries. Dev Biol, 301(2), 504-517. Abstract.  Author URL.
Scholpp S, Wolf O, Brand M, Lumsden A (2006). Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon. Development, 133(5), 855-864. Abstract.  Author URL.
Scholpp S, Brand M (2004). Endocytosis controls spreading and effective signaling range of Fgf8 protein. Curr Biol, 14(20), 1834-1841. Abstract.  Author URL.
Scholpp S, Groth C, Lohs C, Lardelli M, Brand M (2004). Zebrafish fgfr1 is a member of the fgf8 synexpression group and is required for fgf8 signalling at the midbrain-hindbrain boundary. Dev Genes Evol, 214(6), 285-295. Abstract.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon. Development, 130(20), 4881-4893. Abstract.  Author URL.
Scholpp S, Brand M (2003). Integrity of the midbrain region is required to maintain the diencephalic-mesencephalic boundary in zebrafish no isthmus/pax2.1 mutants. Dev Dyn, 228(3), 313-322. Abstract.  Author URL.
Picker A, Scholpp S, Böhli H, Takeda H, Brand M (2002). A novel positive transcriptional feedback loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines. Development, 129(13), 3227-3239. Abstract.  Author URL.
Scholpp S, Brand M (2001). Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain--hindbrain boundary development. Genesis, 30(3), 129-133. Author URL.

Chapters

Rogers S, Scholpp S (2021). Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells. In  (Ed) , 183-190.  Abstract.  Author URL.
Scholpp S, Shimogori T (2013). Building the gateway to consciousness-about the development of the thalamus. In  (Ed) .  Author URL.
Scholpp S, Brand M (2009). Neural Patterning: Midbrain-Hindbrain Boundary. In  (Ed) Encyclopedia of Neuroscience, 205-211.  Abstract.

Conferences

Mattes B, Scholpp S (2017). Towards deciphering the molecular mechanism regulating Wnt ligand trafficking.  Author URL.
Reinartz I, Sinner C, Stanganello E, Mattes B, Scholpp S, Schug A (2016). 3D Simulations of Morphogen Transport in an Early Fish Embryo.  Author URL.
Sinner C, Reinartz I, Boesze B, Scholpp S, Schug A (2016). Dynamic Simulations of Cell Migration with Applications to Brain Development.  Author URL.
Heeren-Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2016). In vivo analysis of formation and endocytosis of the Wnt/beta-Catenin signaling complex in zebrafish embryos.  Author URL.
Sinner C, Stanganello E, Hagemann AIH, Mattes B, Meyen D, Weber S, Raz E, Scholpp S, Schug A (2015). Monte Carlo Simulation of Wnt Propagation by a Novel Transport Mechanism Complementing a Joint Experimental Study.  Author URL.
Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Davidson G, Kirchhausen T, Scholpp S (2014). In-vivo analysis of formation and endocytosis of the Wnt/beta-catenin signaling complex in zebrafish embryos.  Author URL.
Hagemann AI, Schindler S, Scholpp S (2010). The clathrin adaptor-protein subunit ap2m1 regulates canonical Wnt signalling in early neural development of the zebrafish.  Author URL.
Heinze KG, Schlopp S, Brand M, Schwille P (2004). Probing intercellular pathways and propagation of Fgf8 signalling protein during embryogenesis by FCS.  Author URL.
Scholpp S, Brand M (2003). Endocytosis controls propagation of Fgf8 during zebrafish embryogenesis.  Author URL.

Reports

Scholpp S, Poggi L, Zigman M (2013). Brain on the stage - spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013.  Abstract.  Author URL.
Blackshaw S, Scholpp S, Placzek M, Ingraham H, Simerly R, Shimogori T (2010). Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation.  5 pages.  Abstract.  Author URL.

Publications by year


In Press

Gardilla AC, Sanchez D, Brunt L, Scholpp S (In Press). From top to bottom: Cell polarity in Hedgehog and Wnt trafficking. BMC Biology
Liu T-L, Upadhyayula S, Milkie D, Singh V, Wang K, Swineburn I, Scholpp S, Megason S, Kirchhausen T, Betzig E, et al (In Press). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science
Routledge D, Rogers S, Ashktorab H, Phesse TJ, Scholpp S (In Press). The scaffolding protein Flot2 regulates cytoneme-based transport of Wnt3 in gastric cancer.  Abstract.
Brunt L, Greicius G, Evans BD, Virshup DM, Wedgwood KCA, Scholpp S (In Press). Vangl2 regulates the dynamics of Wnt cytonemes in vertebrates.  Abstract.

2022

Winter MJ, Ono Y, Ball JS, Walentinsson A, Michaelsson E, Tochwin A, Scholpp S, Tyler CR, Rees S, Hetheridge MJ, et al (2022). A Combined Human in Silico and CRISPR/Cas9-Mediated in Vivo Zebrafish Based Approach to Provide Phenotypic Data for Supporting Early Target Validation. Frontiers in Pharmacology, 13
Rogers S, Zhang C, Anagnostidis V, Fishel M, Gielen F, Scholpp S (2022). Cancer-associated fibroblast-derived ROR2 induces WNT/PCP activation and polarized migration in receiving gastric cancer cells.
Zhang C, Brunt L, Rogers S, Scholpp S (2022). Cytoneme-mediated transport of active Wnt5b/Ror2 complexes in zebrafish gastrulation.
Routledge D, Rogers S, Ono Y, Brunt L, Meniel V, Tornillo G, Ashktorab H, Phesse TJ, Scholpp S (2022). The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer. eLife, 11 Abstract.
Rogers S, Scholpp S (2022). Vertebrate Wnt5a – at the crossroads of cellular signalling. Seminars in Cell & Developmental Biology, 125, 3-10.

2021

Rogers S, Scholpp S (2021). Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells. In  (Ed) , 183-190.  Abstract.  Author URL.
Sutton G, Kelsh RN, Scholpp S (2021). Review: the Role of Wnt/β-Catenin Signalling in Neural Crest Development in Zebrafish. Frontiers in Cell and Developmental Biology, 9 Abstract.
Brunt L, Greicius G, Rogers S, Evans BD, Virshup DM, Wedgwood KCA, Scholpp S (2021). Vangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling. Nat Commun, 12(1). Abstract.  Author URL.

2020

Cavallo JC, Scholpp S, Flegg MB (2020). Delay-driven oscillations via Axin2 feedback in the Wnt/β-catenin signalling pathway. J Theor Biol, 507 Abstract.  Author URL.
Alshami IJJ, Ono Y, Correia A, Hacker C, Lange A, Scholpp S, Kawasaki M, Ingham PW, Kudoh T (2020). Development of the electric organ in embryos and larvae of the knifefish, Brachyhypopomus gauderio. Developmental Biology, 466(1-2), 99-108.
Scholpp S (2020). Introduction: in vivo cell biology in zebrafish. Histochem Cell Biol, 154(5), 457-461. Author URL.
Rosenbauer J, Zhang C, Mattes B, Reinartz I, Wedgwood K, Schindler S, Sinner C, Scholpp S, Schug A (2020). Modeling of Wnt-mediated tissue patterning in vertebrate embryogenesis. PLOS Computational Biology, 16(6), e1007417-e1007417.
Bosze B, Ono Y, Mattes B, Sinner C, Gourain V, Thumberger T, Tlili S, Wittbrodt J, Saunders TE, Strähle U, et al (2020). Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish. Histochemistry and Cell Biology, 154(5), 463-480. Abstract.
Dawes ML, Soeller C, Scholpp S (2020). Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo. HISTOCHEMISTRY AND CELL BIOLOGY, 154(5), 507-519. Author URL.
Porter L (2020). Wnt signalling and peroxisome dynamics in the zebrafish (Danio rerio).  Abstract.

2019

Zhang C, Scholpp S (2019). Cytonemes in development. Current Opinion in Genetics and Development, 57, 25-30. Abstract.
Routledge D, Scholpp S (2019). Mechanisms of intercellular Wnt transport. Development, 146(10). Abstract.  Author URL.

2018

Mattes B, Scholpp S (2018). Emerging role of contact-mediated cell communication in tissue development and diseases. Histochemistry and Cell Biology, 150, 431-442. Abstract.
Brunt L, Scholpp S (2018). The function of endocytosis in Wnt signaling. Cellular and Molecular Life Sciences, 75(5), 785-795. Abstract.
Scholpp S (2018). Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates. eLife

2017

Mattes B, Scholpp S (2017). Towards deciphering the molecular mechanism regulating Wnt ligand trafficking.  Author URL.

2016

Reinartz I, Sinner C, Stanganello E, Mattes B, Scholpp S, Schug A (2016). 3D Simulations of Morphogen Transport in an Early Fish Embryo.  Author URL.
Sinner C, Reinartz I, Boesze B, Scholpp S, Schug A (2016). Dynamic Simulations of Cell Migration with Applications to Brain Development.  Author URL.
Heeren-Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2016). In vivo analysis of formation and endocytosis of the Wnt/beta-Catenin signaling complex in zebrafish embryos.  Author URL.
Stanganello E, Scholpp S (2016). Role of cytonemes in Wnt transport. J Cell Sci, 129(4), 665-672. Abstract.  Author URL.
Brinkmann E-M, Mattes B, Kumar R, Hagemann AIH, Gradl D, Scholpp S, Steinbeisser H, Kaufmann LT, Özbek S (2016). Secreted Frizzled-related Protein 2 (sFRP2) Redirects Non-canonical Wnt Signaling from Fz7 to Ror2 during Vertebrate Gastrulation. J Biol Chem, 291(26), 13730-13742. Abstract.  Author URL.

2015

Stanganello E, Hagemann AIH, Mattes B, Sinner C, Meyen D, Weber S, Schug A, Raz E, Scholpp S (2015). Filopodia-based Wnt transport during vertebrate tissue patterning. Nat Commun, 6 Abstract.  Author URL.
Sinner C, Stanganello E, Hagemann AIH, Mattes B, Meyen D, Weber S, Raz E, Scholpp S, Schug A (2015). Monte Carlo Simulation of Wnt Propagation by a Novel Transport Mechanism Complementing a Joint Experimental Study.  Author URL.
Hirschbiel AF, Geyer S, Yameen B, Welle A, Nikolov P, Giselbrecht S, Scholpp S, Delaittre G, Barner-Kowollik C (2015). Photolithographic patterning of 3D-formed polycarbonate films for targeted cell guiding. Adv Mater, 27(16), 2621-2626. Abstract.  Author URL.

2014

Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). Correction to in vivo analysis of formation and endocytosis of the Wnt/β-Catenin signaling complex in zebrafish embryos [J. Cell Sci. 127, (2014) 3970-3982]. Journal of Cell Science, 127(24).
Rengarajan C, Matzke A, Reiner L, Orian-Rousseau V, Scholpp S (2014). Endocytosis of Fgf8 is a double-stage process and regulates spreading and signaling. PLoS One, 9(1). Abstract.  Author URL.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-Catenin signaling complex in zebrafish embryos. Development, 141(19), e1907-e1907.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-catenin signaling complex in zebrafish embryos. J Cell Sci, 127(Pt 18), 3970-3982. Abstract.  Author URL.
Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Davidson G, Kirchhausen T, Scholpp S (2014). In-vivo analysis of formation and endocytosis of the Wnt/beta-catenin signaling complex in zebrafish embryos.  Author URL.
Chatterjee M, Guo Q, Weber S, Scholpp S, Li JY (2014). Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shh in the diencephalon in vertebrates. BMC Biol, 12 Abstract.  Author URL.
Chen Q, Su Y, Wesslowski J, Hagemann AI, Ramialison M, Wittbrodt J, Scholpp S, Davidson G (2014). Tyrosine phosphorylation of LRP6 by Src and Fer inhibits Wnt/β-catenin signalling. EMBO Rep, 15(12), 1254-1267. Abstract.  Author URL.

2013

Scholpp S, Poggi L, Zigman M (2013). Brain on the stage - spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013.  Abstract.  Author URL.
Scholpp S, Shimogori T (2013). Building the gateway to consciousness-about the development of the thalamus. In  (Ed) .  Author URL.
Efremov AN, Stanganello E, Welle A, Scholpp S, Levkin PA (2013). Micropatterned superhydrophobic structures for the simultaneous culture of multiple cell types and the study of cell-cell communication. Biomaterials, 34(7), 1757-1763. Abstract.  Author URL.
Schmidt R, Strähle U, Scholpp S (2013). Neurogenesis in zebrafish - from embryo to adult. Neural Dev, 8 Abstract.  Author URL.

2012

Hagemann AIH, Scholpp S (2012). The Tale of the Three Brothers - Shh, Wnt, and Fgf during Development of the Thalamus. Front Neurosci, 6 Abstract.  Author URL.
Mattes B, Weber S, Peres J, Chen Q, Davidson G, Houart C, Scholpp S (2012). Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Dev, 7 Abstract.  Author URL.

2011

Peukert D, Weber S, Lumsden A, Scholpp S (2011). Lhx2 and Lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating Wnt signaling. PLoS Biol, 9(12). Abstract.  Author URL.

2010

Scholpp S, Lumsden A (2010). Building a bridal chamber: development of the thalamus. Trends Neurosci, 33(8), 373-380. Abstract.  Author URL.
Blackshaw S, Scholpp S, Placzek M, Ingraham H, Simerly R, Shimogori T (2010). Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation.  5 pages.  Abstract.  Author URL.
Peukert D, Scholpp S (2010). Ontogenesis of the brain: the development of the thalamus - the gateway to consciousness. BioSpektrum, 16(6), 639-643. Abstract.
Hagemann AI, Schindler S, Scholpp S (2010). The clathrin adaptor-protein subunit ap2m1 regulates canonical Wnt signalling in early neural development of the zebrafish.  Author URL.
Fassier C, Hutt JA, Scholpp S, Lumsden A, Giros B, Nothias F, Schneider-Maunoury S, Houart C, Hazan J (2010). Zebrafish atlastin controls motility and spinal motor axon architecture via inhibition of the BMP pathway. Nat Neurosci, 13(11), 1380-1387. Abstract.  Author URL.

2009

Yu SR, Burkhardt M, Nowak M, Ries J, Petrásek Z, Scholpp S, Schwille P, Brand M (2009). Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules. Nature, 461(7263), 533-536. Abstract.  Author URL.
Scholpp S, Delogu A, Gilthorpe J, Peukert D, Schindler S, Lumsden A (2009). Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus. Proc Natl Acad Sci U S A, 106(47), 19895-19900. Abstract.  Author URL.
Scholpp S, Brand M (2009). Neural Patterning: Midbrain-Hindbrain Boundary. In  (Ed) Encyclopedia of Neuroscience, 205-211.  Abstract.

2007

Wendl T, Adzic D, Schoenebeck JJ, Scholpp S, Brand M, Yelon D, Rohr KB (2007). Early developmental specification of the thyroid gland depends on han-expressing surrounding tissue and on FGF signals. Development, 134(15), 2871-2879. Abstract.  Author URL.
Scholpp S (2007). Hedgehogs, Fluorescence Imaging & Brain Development. infocus Magazine, 66-75.
Scholpp S, Foucher I, Staudt N, Peukert D, Lumsden A, Houart C (2007). Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development, 134(17), 3167-3176. Abstract.  Author URL.
Erickson T, Scholpp S, Brand M, Moens CB, Waskiewicz AJ (2007). Pbx proteins cooperate with Engrailed to pattern the midbrain-hindbrain and diencephalic-mesencephalic boundaries. Dev Biol, 301(2), 504-517. Abstract.  Author URL.

2006

Scholpp S, Wolf O, Brand M, Lumsden A (2006). Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon. Development, 133(5), 855-864. Abstract.  Author URL.

2004

Scholpp S, Brand M (2004). Endocytosis controls spreading and effective signaling range of Fgf8 protein. Curr Biol, 14(20), 1834-1841. Abstract.  Author URL.
Heinze KG, Schlopp S, Brand M, Schwille P (2004). Probing intercellular pathways and propagation of Fgf8 signalling protein during embryogenesis by FCS.  Author URL.
Scholpp S, Groth C, Lohs C, Lardelli M, Brand M (2004). Zebrafish fgfr1 is a member of the fgf8 synexpression group and is required for fgf8 signalling at the midbrain-hindbrain boundary. Dev Genes Evol, 214(6), 285-295. Abstract.  Author URL.

2003

Scholpp S, Brand M (2003). Endocytosis controls propagation of Fgf8 during zebrafish embryogenesis.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon. Development, 130(20), 4881-4893. Abstract.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Erratum: Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon (Development vol. 130 (4881-4893)). Development, 130(21).
Scholpp S, Brand M (2003). Integrity of the midbrain region is required to maintain the diencephalic-mesencephalic boundary in zebrafish no isthmus/pax2.1 mutants. Dev Dyn, 228(3), 313-322. Abstract.  Author URL.

2002

Picker A, Scholpp S, Böhli H, Takeda H, Brand M (2002). A novel positive transcriptional feedback loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines. Development, 129(13), 3227-3239. Abstract.  Author URL.

2001

Scholpp S, Brand M (2001). Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain--hindbrain boundary development. Genesis, 30(3), 129-133. Author URL.

Steffen_Scholpp Details from cache as at 2022-12-04 14:25:50

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External Engagement and Impact

Administrative responsibilities

  • Chair of the LSI Lab Management Group (LMG)
  • Deputy Director of Postgraduate Research (D-DPGR)
  • Co-organiser of the Biosciences Seminar Series

Committee/panel activities

  • Panel member of the BBSRC committee (since 2022)
  • Member of the reviewing board for the National Science Centre, NSC, Poland (since 2019)

Editorial responsibilities

  • Scientific editor of Mechanisms of Development (MOD) (2016 - present)
  • Scientific editor of Genesis, John Wiley and Sons, Inc (2015 - present)
  • Associated editor of Frontiers in Neuroscience (2011 - present)

External doctoral examining nationally and internationally

  • PhD       Maastricht, The Netherlands; Supervisor Dr Stefan Giiselbrecht, 2022
  • PhD       Francis Crick, UK; Supervisor Prof Jim Smith, 2021
  • PhD       iBV, Nice U, France; Supervisor Dr M. Fürthauer, 2021
  • PhD       U Nottingham, UK; Supervisor Prof Martin Gehring, 2019
  • PhD       Oxford Brookes U., UK; Supervisor Prof Alistair McGregor, 2019
  • MPhil     U Bristol, UK; Supervisor Dr Beck Richardson, 2018

Invited lectures

Number of invited lectures in total

International lectures

National lectures

over 60

over 40

over 20


Workshops/Conferences organised

2022                Organizer, EMBO workshop on "Long-distance cell.-cell communication", Exeter, UK.

2020                Organizer, Southwest Zebrafish Meeting 2020, Exeter, UK.

2013                Organizer, EMBO practical course „Imaging of Neural Development“, KIT, Germany.

                        Lecturer, Summer School, Jap Soc Dev Biol, Tokyo, Japan.

2012                Lecturer, GfE Summer School, Schloss Reissenburg, Ulm, Germany.

    

Happy Lab Citizen

Teaching

I have taught at the undergraduate and graduate level, including general biology, cell biology, developmental biology, and neurobiology at the Karlsruhe Institute of Technology and the University of Exeter. I have also personally trained over 20 undergraduates in research, some of whom have been my co-authors on peer-reviewed publications.


Teaching is a privilege, and so I try to practice teaching the most effective way to optimize student learning. I enjoy training students to learn science by doing science, both in terms of hands-on research activities and courses that emphasize formulating research questions, critical analysis of data, and drawing meaningful conclusions. My goal is to teach the subject matter, but also to teach students how to train themselves to learn.  As module coordinator, I emphasize organization and context while being mindful of how different students learn and what techniques work best both inside and outside of contact hours, in the classroom or the lab.


I have received several teaching awards at the Karlsruhe Institute of Technology, including the German Certificate for Higher Education (Baden-Württemberg Zertifikat fur Hochschuldidaktik, 200h) in 2012. In 2013, I was awarded the Certificate for Academic Leadership (3y, 160h) to guide working groups in an efficient and target-orientated way.

Modules

2022/23


The Scholpp lab in 2020

Supervision / Group

Postdoctoral researchers

  • Lucy Brunt
  • Yosuke Ono
  • Tom Piers
  • Sally Rogers

Postgraduate researchers

  • Michael Dawes (BBSRC DTP Student)
  • Joshua Donnelly
  • Kevin Fang (Chinese Scholarship Council, CSC, Student)
  • Daniel Routledge (MRC DTP Student)
  • Gemma Sutton (BBSRC DTP Student)
  • Chengting Zhang (Chinese Scholarship Council, CSC, Student)

Research Technicians

  • Agnieszka Kaczmar

Alumni

  • Bernadett Boesze PhD student (2012-2016)
  • Holly Elson
  • Simone Geyer PhD student(2012-2015)
  • Anja Hagemann PostDoc (2009-2014)
  • Benjamin Mattes PhD student (2015-2018)
  • Daniela Peukert PhD student(2008-2011)
  • Lauren Porter MSc student(2018-2019)
  • Charanya Rengarajan PhD student (2009-2013)
  • Simone Schindler Technician (2017-2018)
  • Eliana Stanganello PhD student, summa cum laude (2011-2015)
  • Joana Viana PostDoc (2018-2019)

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