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Dr Steffen Scholpp

Dr Steffen Scholpp

Associate Professor, Cell and Developmental Biology

 7451

 +44 (0)1392 727451

 Living Systems Institute T02.12

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

Tissue development is a key process for life starting from the earliest embryonic stages during which cells differentiate into later organs composing an entire body. An essential component for these developmental processes but also for tissue regeneration and stem cell regulation is the communication of cells by chemical signalling. The highly conserved family of Wnt proteins represents important regulators of cell behaviour, tissue development and homeostasis by inducing responses in a concentration dependent manner. We identified a novel way of spreading of Wnt proteins in vertebrates: Wnt molecules are mobilized on specific cell protrusions so-called cytonemes. These specialized signalling filopodia transmit signal proteins between communicating cells and allow a high degree of control of propagation speed, direction and concentration of the transmitted ligand. The signalling molecules are delivered directly to the receiving cells by a direct-contact model and parameters such as cytoneme length or speed of filopodia formation dictate local Wnt concentration. At the Living Systems Institute, we collaborate with biophysicists using super-resolution microscopy to describe these signalling processes in a quantitative way on a molecular level. As it is very difficult to determine the specific impact of individual parameters in a complex biological system by a purely experimental approach, we interact with mathematicians using computational modelling. Together, we develop a robust mathematical model for the distribution of signal molecules on the basis of signalling filopodia. Due to the conserved nature of vertebrate cell behaviour our results will be relevant to Wnt signalling during human embryonic development and could suggest novel vulnerabilities to Wnt-dependent diseases – a prerequisite for the development of novel therapeutics.

Qualifications

2009-2016 Emmy-Noether Research Group Leader (Assitant Professor)

2003 PhD Neurobiology (Hons., summa cum laude), University of Heidelberg, Germany

Career

2017-present Associate Professor of Cell and Developmental Biology, Biosciences, University of Exeter, UK

2009-2016 Emmy-Noether group leader (Assistant Professor) at the Karlsruhe Institute of Technology (KIT), Germany

2004-2009 Postdoctoral Research Fellow with Andrew Lumsden, MRC Centre for Developmental Neurobiology, King’s College London, UK

2003-2004 Postdoctoral Research Associate with M. Brand, Max Planck-Institute of Cell Biology and Genetics (MPI-CBG), Dresden, Germany

2003 PhD Neurobiology (Hons., summa cum laude), University of Heidelberg, Germany

1999-2003 PhD student in Neurobiology (Laboratory of M. Brand), University of Heidelberg, Germany and Max Planck-Institute of Cell Biology and Genetics (MPI-CBG), Dresden, Germany

Links

Wnt protein (red) on cytoneme tips in vivo

Research

Research interests

After secretion, developmental signals known as morphogens must travel relatively long distances to form a concentration gradient that the responding tissue uses to acquire positional information. The role of morphogen transport and endocytic trafficking in this process is the subject of intense debate. Wnt proteins regulate developmental processes, tissue regeneration and stem cell maintenance. It has been postulated that Wnt/β-catenin signalling form concentration gradients across responsive tissues and act as morphogens. However, little is known about the transport mechanism for these lipid-modified signalling proteins in vertebrates.

Recently, we showed that Wnt8a is transported on short, actin-based filopodia to contact responding cells and activate signalling during neural plate formation in zebrafish (1).Wnt/ Ror2 signalling regulates the formation of these Wnt-positive filopodia (5). Enhanced formation of filopodia increases the effective signalling range of Wnt by facilitating spreading. Consistently, reduction in filopodia leads to a restricted distribution of the ligand and a limited signalling range. Using a numerical simulation, we provide evidence that such a short-range transport system for Wnt has long-range signalling function.

After contact by Wnt/β-catenin positive filopodia, a multi-protein complex at the plasma membrane assembles clustering membrane-bound receptors and intracellular signal transducers into the so-called Lrp6-signalosome. Our imaging studies in live zebrafish embryos showed that the signalosome is a highly dynamic structure, which is continuously assembled and disassembled by a Dvl2-mediated endocytic process (2). We showed that this endocytic process is not only essential for ligand-receptor internalization but also for signaling.

We conclude that a cytoneme-based transport system for Wnt and subsequent endocytosis is important for Wnt/β-catenin signaling and controls anteroposterior patterning of the neural plate during vertebrate gastrulation (3,4).

(1) Stanganello et al., Nature Comms., 2015; (2) Hagemann, et al., J.Cell Sci., 2014; (3) Stanganello and Scholpp, J.Cell Sci., 2016; (4) Brunt and Scholpp, CMLS, 2017; (5) Mattes et al., eLife, 2018.

Research projects

OPEN POSITIONS:

We're recruiting talented graduates from all areas of bioscience to become MSc or PhD students in our lab.

1) BBSRC DTP PhD project (fully-funded four-year SWBio DTP studentship)

Dissecting the function of Wnt signalling in zebrafish neural crest development. (SWBio DTP)

Supervisor team: Steffen Scholpp and Robert Kelsh (Bath)

 

Project Description

The neural crest (NC) is a stem-cell-like cell population, which is unique to vertebrates. NC cells can become peripheral neurons and glia, pigment cells and cartilage. NC cells migrate a long distance from their birthplace to the site where they function. Along the way, NC cells are exposed to signals promoting the variety of cell fates. We have shown that Wnt signals regulate the balance between peripheral neurons and pigment cells (Vibert et al., 2017 Pigm Cell Melanoma). However, how Wnt activates pigment cell fate, and leaving others to adopt another fate, is not understood. Recently, we could show that Wnt transport is directed by long cellular extensions called cytonemes. (Stanganello et al., 2015; Nat Comms). The producing cell loads Wnt on cytonemes to send them to the receiving cell. So, the producers can control how much and how far Wnt is transported, but also which cells are contacted. We hypothesise that Wnt cytonemes are the underlying cellular mechanism driving diversification of the NC lineage.

Under the supervision of leading cell biologists in Exeter and Bath, the student will test this hypothesis by studying Wnt transport in zebrafish. The student will generate transgenic zebrafish lines with fluorescently tagged Wnt proteins. The student will use these fish lines to monitor Wnt trafficking by advanced high- and super-resolution microscopy. Simultaneously signal activation in the receiving neural crest cells will be described by using real-time PCR, and live reporter systems. Finally, the student will interfere with Wnt cytonemes by using chemical inhibitors and CRISPR-based mutations in zebrafish to study their impact on NC differentiation.

The student selected for this project will develop invaluable skill sets in experimental genetics, cell biology while also making a significant contribution to the development of and high-resolution in vivo imaging techniques. This combined skill set will make the candidate a highly desirable recruitment prospect for future academic and industrial employers.

The Living Systems Institute in Exeter and the Department of Biology in Bath, with complementary expertise in biosciences and high-resolution imaging, will be an optimal environment to conduct these doctoral training studies. We offer unique training opportunities for the PhD student as it allows the student to address critical problems in life sciences with state-of-the-art equipment in an interdisciplinary environment.

Further details here  https://www.findaphd.com/phds/project/dissecting-the-function-of-wnt-signalling-in-zebrafish-neural-crest-development-phd-in-biosciences-swbio-dtp/?p113928 and here https://www.swbio.ac.uk/programme/

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2) PhD project for the LSI PhD programme

Quantitative analysis of Wnt pathway components at cytoneme contact sites in the living zebrafish embryo by super-resolution microscopy

Supervisor team: Steffen Scholpp, Christian Soeller, Kyle Wedgwood

Project Description

Cell-cell communication is essential for the regulation of the development and homeostasis of all multicellular organisms. Secreted signals like Wnt signals regulate fundamental processes including cell proliferation and differentiation, cell polarity and migration. Misregulation of this signalling network causes severe diseases e.g. cancer, neurodegenerative diseases, and osteoporosis. Binding of a Wnt ligand with a seven-pass transmembrane Frizzled (Fzd) receptor on the surface of cells is the principal way to activate the Wnt signalling network. 19 different Wnt ligands may bind to 10 different Fzd receptors to specify the nature of the downstream signalling event. Accurate quantification of these interactions in living organisms is crucial to understand signalling specificity, however, due to technical obstacles, this was not possible until today.

In collaboration with Leica Microsystems and under the supervision of cell biologists and biophysicists, we will employ correlation spectroscopy to quantify for the first time the effective binding affinities - Kd (effective) - between Wnt ligands and Fzd proteins in vivo, which have important functions in early vertebrate development. The student will apply molecular biological techniques such as CRISPR/Cas9- based knock-out methods and super-resolution microscopy techniques such as PALM/dSTORM to visualize and quantify ligands and receptors in zebrafish. Then we will use imaging-based measurements of ligand-receptor interaction in the zebrafish gastrula by fluorescence fluctuation techniques.

The student selected for this interdisciplinary project will develop invaluable skill sets in experimental genetics, in vivo techniques, biophysical methods such as spectroscopy, super-resolution microscopy and mathematical modelling during three rotation projects in the first year. This combined skill set including the interactions with industrial partners will prepare the student for a successful PhD project and make the candidate a highly desirable recruitment prospect for future academic and industrial employers.

The Living Systems Institute (LSI), with complementary expertise in biosciences and physics will be an optimal environment to conduct these doctoral training studies. The LSI offers unique training opportunities for the PhD student in its newly funded PhD programme as it allows the student to address key problems in life sciences with state-of-the-art equipment in an interdisciplinary environment. The project includes close collaboration with the universities of Cardiff, Bath and Bristol to complement the required skill sets

 

Join our team!

Research grants

  • 2019 Medical Research Council
    Deciphering the molecular mechanism of Wnt trafficking in gastric cancer
  • 2019 BBSRC
    Quantitative analysis of cytoneme-based Wnt trafficking and signalling in vivo
  • 2019 BBSRC
    HPF to enable a high-quality ultrastructural analysis of biological samples
  • 2018 BBSRC
    A Single Molecule Detection Unit to perform in vivo FCS and FLIM-FRET analysis in zebrafish
  • 2017 Wellcome Trust
    CBMA Seed Corn Award

Publications

Key publications | Publications by category | Publications by year

Key publications


Liu T-L, Upadhyayula S, Milkie D, Singh V, Wang K, Swineburn I, Scholpp S, Megason S, Kirchhausen T, Betzig E, et al (In Press). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science
Mattes B, Scholpp S (2018). Emerging role of contact-mediated cell communication in tissue development and diseases. Histochemistry and Cell Biology, 150, 431-442. Abstract.  Full text.
Scholpp S (2018). Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates. eLife Full text.
Stanganello E, Scholpp S (2016). Role of cytonemes in Wnt transport. J Cell Sci, 129(4), 665-672. Abstract.  Author URL.
Stanganello E, Hagemann AIH, Mattes B, Sinner C, Meyen D, Weber S, Schug A, Raz E, Scholpp S (2015). Filopodia-based Wnt transport during vertebrate tissue patterning. Nat Commun, 6 Abstract.  Author URL.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-catenin signaling complex in zebrafish embryos. J Cell Sci, 127(Pt 18), 3970-3982. Abstract.  Author URL.

Publications by category


Journal articles

Gardilla AC, Sanchez D, Brunt L, Scholpp S (In Press). From top to bottom: Cell polarity in Hedgehog and Wnt trafficking. BMC Biology Full text.
Liu T-L, Upadhyayula S, Milkie D, Singh V, Wang K, Swineburn I, Scholpp S, Megason S, Kirchhausen T, Betzig E, et al (In Press). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science
Zhang C, Scholpp S (2019). Cytonemes in development. Current Opinion in Genetics and Development, 57, 25-30. Abstract.
Routledge D, Scholpp S (2019). Mechanisms of intercellular Wnt transport. Development, 146(10). Abstract.  Author URL.
Mattes B, Scholpp S (2018). Emerging role of contact-mediated cell communication in tissue development and diseases. Histochemistry and Cell Biology, 150, 431-442. Abstract.  Full text.
Brunt L, Scholpp S (2018). The function of endocytosis in Wnt signaling. Cellular and Molecular Life Sciences, 75(5), 785-795. Abstract.  Full text.
Scholpp S (2018). Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates. eLife Full text.
Stanganello E, Scholpp S (2016). Role of cytonemes in Wnt transport. J Cell Sci, 129(4), 665-672. Abstract.  Author URL.
Brinkmann E-M, Mattes B, Kumar R, Hagemann AIH, Gradl D, Scholpp S, Steinbeisser H, Kaufmann LT, Ă–zbek S (2016). Secreted Frizzled-related Protein 2 (sFRP2) Redirects Non-canonical Wnt Signaling from Fz7 to Ror2 during Vertebrate Gastrulation. J Biol Chem, 291(26), 13730-13742. Abstract.  Author URL.  Full text.
Stanganello E, Hagemann AIH, Mattes B, Sinner C, Meyen D, Weber S, Schug A, Raz E, Scholpp S (2015). Filopodia-based Wnt transport during vertebrate tissue patterning. Nat Commun, 6 Abstract.  Author URL.
Hirschbiel AF, Geyer S, Yameen B, Welle A, Nikolov P, Giselbrecht S, Scholpp S, Delaittre G, Barner-Kowollik C (2015). Photolithographic patterning of 3D-formed polycarbonate films for targeted cell guiding. Adv Mater, 27(16), 2621-2626. Abstract.  Author URL.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). Correction to in vivo analysis of formation and endocytosis of the Wnt/β-Catenin signaling complex in zebrafish embryos [J. Cell Sci. 127, (2014) 3970-3982]. Journal of Cell Science, 127(24).
Rengarajan C, Matzke A, Reiner L, Orian-Rousseau V, Scholpp S (2014). Endocytosis of Fgf8 is a double-stage process and regulates spreading and signaling. PLoS One, 9(1). Abstract.  Author URL.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-catenin signaling complex in zebrafish embryos. J Cell Sci, 127(Pt 18), 3970-3982. Abstract.  Author URL.
Chatterjee M, Guo Q, Weber S, Scholpp S, Li JY (2014). Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shh in the diencephalon in vertebrates. BMC Biol, 12 Abstract.  Author URL.
Chen Q, Su Y, Wesslowski J, Hagemann AI, Ramialison M, Wittbrodt J, Scholpp S, Davidson G (2014). Tyrosine phosphorylation of LRP6 by Src and Fer inhibits Wnt/β-catenin signalling. EMBO Rep, 15(12), 1254-1267. Abstract.  Author URL.
Scholpp S, Poggi L, Zigman M (2013). Brain on the stage - spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013. Neural Dev, 8 Abstract.  Author URL.
Scholpp S, Shimogori T (2013). Building the gateway to consciousness-about the development of the thalamus. Front Neurosci, 7 Author URL.
Efremov AN, Stanganello E, Welle A, Scholpp S, Levkin PA (2013). Micropatterned superhydrophobic structures for the simultaneous culture of multiple cell types and the study of cell-cell communication. Biomaterials, 34(7), 1757-1763. Abstract.  Author URL.
Schmidt R, Strähle U, Scholpp S (2013). Neurogenesis in zebrafish - from embryo to adult. Neural Dev, 8 Abstract.  Author URL.
Hagemann AIH, Scholpp S (2012). The Tale of the Three Brothers - Shh, Wnt, and Fgf during Development of the Thalamus. Front Neurosci, 6 Abstract.  Author URL.
Mattes B, Weber S, Peres J, Chen Q, Davidson G, Houart C, Scholpp S (2012). Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Dev, 7 Abstract.  Author URL.
Peukert D, Weber S, Lumsden A, Scholpp S (2011). Lhx2 and Lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating Wnt signaling. PLoS Biol, 9(12). Abstract.  Author URL.
Scholpp S, Lumsden A (2010). Building a bridal chamber: development of the thalamus. Trends Neurosci, 33(8), 373-380. Abstract.  Author URL.
Blackshaw S, Scholpp S, Placzek M, Ingraham H, Simerly R, Shimogori T (2010). Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation. J Neurosci, 30(45), 14925-14930. Abstract.  Author URL.
Peukert D, Scholpp S (2010). Ontogenesis of the brain: the development of the thalamus - the gateway to consciousness. BioSpektrum, 16(6), 639-643. Abstract.
Fassier C, Hutt JA, Scholpp S, Lumsden A, Giros B, Nothias F, Schneider-Maunoury S, Houart C, Hazan J (2010). Zebrafish atlastin controls motility and spinal motor axon architecture via inhibition of the BMP pathway. Nat Neurosci, 13(11), 1380-1387. Abstract.  Author URL.
Yu SR, Burkhardt M, Nowak M, Ries J, Petrásek Z, Scholpp S, Schwille P, Brand M (2009). Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules. Nature, 461(7263), 533-536. Abstract.  Author URL.
Scholpp S, Delogu A, Gilthorpe J, Peukert D, Schindler S, Lumsden A (2009). Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus. Proc Natl Acad Sci U S A, 106(47), 19895-19900. Abstract.  Author URL.
Wendl T, Adzic D, Schoenebeck JJ, Scholpp S, Brand M, Yelon D, Rohr KB (2007). Early developmental specification of the thyroid gland depends on han-expressing surrounding tissue and on FGF signals. Development, 134(15), 2871-2879. Abstract.  Author URL.
Scholpp S, Foucher I, Staudt N, Peukert D, Lumsden A, Houart C (2007). Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development, 134(17), 3167-3176. Abstract.  Author URL.
Erickson T, Scholpp S, Brand M, Moens CB, Waskiewicz AJ (2007). Pbx proteins cooperate with Engrailed to pattern the midbrain-hindbrain and diencephalic-mesencephalic boundaries. Dev Biol, 301(2), 504-517. Abstract.  Author URL.
Scholpp S, Wolf O, Brand M, Lumsden A (2006). Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon. Development, 133(5), 855-864. Abstract.  Author URL.
Scholpp S, Brand M (2004). Endocytosis controls spreading and effective signaling range of Fgf8 protein. Curr Biol, 14(20), 1834-1841. Abstract.  Author URL.
Scholpp S, Groth C, Lohs C, Lardelli M, Brand M (2004). Zebrafish fgfr1 is a member of the fgf8 synexpression group and is required for fgf8 signalling at the midbrain-hindbrain boundary. Dev Genes Evol, 214(6), 285-295. Abstract.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon (vol 130, pg 4881, 2003). DEVELOPMENT, 130(21), 5293-5293. Author URL.
Scholpp S, Lohs C, Brand M (2003). Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon. Development, 130(20), 4881-4893. Abstract.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Erratum: Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon (Development vol. 130 (4881-4893)). Development, 130(21).
Scholpp S, Brand M (2003). Integrity of the midbrain region is required to maintain the diencephalic-mesencephalic boundary in zebrafish no isthmus/pax2.1 mutants. Dev Dyn, 228(3), 313-322. Abstract.  Author URL.
Picker A, Scholpp S, Böhli H, Takeda H, Brand M (2002). A novel positive transcriptional feedback loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines. Development, 129(13), 3227-3239. Abstract.  Author URL.
Scholpp S, Brand M (2001). Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain--hindbrain boundary development. Genesis, 30(3), 129-133. Author URL.

Chapters

Scholpp S, Brand M (2009). Neural Patterning: Midbrain-Hindbrain Boundary. In  (Ed) Encyclopedia of Neuroscience, 205-211.  Abstract.

Conferences

Mattes B, Scholpp S (2017). Towards deciphering the molecular mechanism regulating Wnt ligand trafficking.  Author URL.
Reinartz I, Sinner C, Stanganello E, Mattes B, Scholpp S, Schug A (2016). 3D Simulations of Morphogen Transport in an Early Fish Embryo.  Author URL.
Sinner C, Reinartz I, Boesze B, Scholpp S, Schug A (2016). Dynamic Simulations of Cell Migration with Applications to Brain Development.  Author URL.
Heeren-Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2016). In vivo analysis of formation and endocytosis of the Wnt/beta-Catenin signaling complex in zebrafish embryos.  Author URL.
Sinner C, Stanganello E, Hagemann AIH, Mattes B, Meyen D, Weber S, Raz E, Scholpp S, Schug A (2015). Monte Carlo Simulation of Wnt Propagation by a Novel Transport Mechanism Complementing a Joint Experimental Study.  Author URL.
Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Davidson G, Kirchhausen T, Scholpp S (2014). In-vivo analysis of formation and endocytosis of the Wnt/beta-catenin signaling complex in zebrafish embryos.  Author URL.
Hagemann AI, Schindler S, Scholpp S (2010). The clathrin adaptor-protein subunit ap2m1 regulates canonical Wnt signalling in early neural development of the zebrafish.  Author URL.
Heinze KG, Schlopp S, Brand M, Schwille P (2004). Probing intercellular pathways and propagation of Fgf8 signalling protein during embryogenesis by FCS.  Author URL.
Scholpp S, Brand M (2003). Endocytosis controls propagation of Fgf8 during zebrafish embryogenesis.  Author URL.

Publications by year


In Press

Gardilla AC, Sanchez D, Brunt L, Scholpp S (In Press). From top to bottom: Cell polarity in Hedgehog and Wnt trafficking. BMC Biology Full text.
Liu T-L, Upadhyayula S, Milkie D, Singh V, Wang K, Swineburn I, Scholpp S, Megason S, Kirchhausen T, Betzig E, et al (In Press). Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms. Science

2019

Zhang C, Scholpp S (2019). Cytonemes in development. Current Opinion in Genetics and Development, 57, 25-30. Abstract.
Routledge D, Scholpp S (2019). Mechanisms of intercellular Wnt transport. Development, 146(10). Abstract.  Author URL.

2018

Mattes B, Scholpp S (2018). Emerging role of contact-mediated cell communication in tissue development and diseases. Histochemistry and Cell Biology, 150, 431-442. Abstract.  Full text.
Brunt L, Scholpp S (2018). The function of endocytosis in Wnt signaling. Cellular and Molecular Life Sciences, 75(5), 785-795. Abstract.  Full text.
Scholpp S (2018). Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates. eLife Full text.

2017

Mattes B, Scholpp S (2017). Towards deciphering the molecular mechanism regulating Wnt ligand trafficking.  Author URL.

2016

Reinartz I, Sinner C, Stanganello E, Mattes B, Scholpp S, Schug A (2016). 3D Simulations of Morphogen Transport in an Early Fish Embryo.  Author URL.
Sinner C, Reinartz I, Boesze B, Scholpp S, Schug A (2016). Dynamic Simulations of Cell Migration with Applications to Brain Development.  Author URL.
Heeren-Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2016). In vivo analysis of formation and endocytosis of the Wnt/beta-Catenin signaling complex in zebrafish embryos.  Author URL.
Stanganello E, Scholpp S (2016). Role of cytonemes in Wnt transport. J Cell Sci, 129(4), 665-672. Abstract.  Author URL.
Brinkmann E-M, Mattes B, Kumar R, Hagemann AIH, Gradl D, Scholpp S, Steinbeisser H, Kaufmann LT, Ă–zbek S (2016). Secreted Frizzled-related Protein 2 (sFRP2) Redirects Non-canonical Wnt Signaling from Fz7 to Ror2 during Vertebrate Gastrulation. J Biol Chem, 291(26), 13730-13742. Abstract.  Author URL.  Full text.

2015

Stanganello E, Hagemann AIH, Mattes B, Sinner C, Meyen D, Weber S, Schug A, Raz E, Scholpp S (2015). Filopodia-based Wnt transport during vertebrate tissue patterning. Nat Commun, 6 Abstract.  Author URL.
Sinner C, Stanganello E, Hagemann AIH, Mattes B, Meyen D, Weber S, Raz E, Scholpp S, Schug A (2015). Monte Carlo Simulation of Wnt Propagation by a Novel Transport Mechanism Complementing a Joint Experimental Study.  Author URL.
Hirschbiel AF, Geyer S, Yameen B, Welle A, Nikolov P, Giselbrecht S, Scholpp S, Delaittre G, Barner-Kowollik C (2015). Photolithographic patterning of 3D-formed polycarbonate films for targeted cell guiding. Adv Mater, 27(16), 2621-2626. Abstract.  Author URL.

2014

Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). Correction to in vivo analysis of formation and endocytosis of the Wnt/β-Catenin signaling complex in zebrafish embryos [J. Cell Sci. 127, (2014) 3970-3982]. Journal of Cell Science, 127(24).
Rengarajan C, Matzke A, Reiner L, Orian-Rousseau V, Scholpp S (2014). Endocytosis of Fgf8 is a double-stage process and regulates spreading and signaling. PLoS One, 9(1). Abstract.  Author URL.
Hagemann AIH, Kurz J, Kauffeld S, Chen Q, Reeves PM, Weber S, Schindler S, Davidson G, Kirchhausen T, Scholpp S, et al (2014). In vivo analysis of formation and endocytosis of the Wnt/β-catenin signaling complex in zebrafish embryos. J Cell Sci, 127(Pt 18), 3970-3982. Abstract.  Author URL.
Hagemann A, Kurz J, Kauffeld S, Chen Q, Reeves P, Davidson G, Kirchhausen T, Scholpp S (2014). In-vivo analysis of formation and endocytosis of the Wnt/beta-catenin signaling complex in zebrafish embryos.  Author URL.
Chatterjee M, Guo Q, Weber S, Scholpp S, Li JY (2014). Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shh in the diencephalon in vertebrates. BMC Biol, 12 Abstract.  Author URL.
Chen Q, Su Y, Wesslowski J, Hagemann AI, Ramialison M, Wittbrodt J, Scholpp S, Davidson G (2014). Tyrosine phosphorylation of LRP6 by Src and Fer inhibits Wnt/β-catenin signalling. EMBO Rep, 15(12), 1254-1267. Abstract.  Author URL.

2013

Scholpp S, Poggi L, Zigman M (2013). Brain on the stage - spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013. Neural Dev, 8 Abstract.  Author URL.
Scholpp S, Shimogori T (2013). Building the gateway to consciousness-about the development of the thalamus. Front Neurosci, 7 Author URL.
Efremov AN, Stanganello E, Welle A, Scholpp S, Levkin PA (2013). Micropatterned superhydrophobic structures for the simultaneous culture of multiple cell types and the study of cell-cell communication. Biomaterials, 34(7), 1757-1763. Abstract.  Author URL.
Schmidt R, Strähle U, Scholpp S (2013). Neurogenesis in zebrafish - from embryo to adult. Neural Dev, 8 Abstract.  Author URL.

2012

Hagemann AIH, Scholpp S (2012). The Tale of the Three Brothers - Shh, Wnt, and Fgf during Development of the Thalamus. Front Neurosci, 6 Abstract.  Author URL.
Mattes B, Weber S, Peres J, Chen Q, Davidson G, Houart C, Scholpp S (2012). Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain. Neural Dev, 7 Abstract.  Author URL.

2011

Peukert D, Weber S, Lumsden A, Scholpp S (2011). Lhx2 and Lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating Wnt signaling. PLoS Biol, 9(12). Abstract.  Author URL.

2010

Scholpp S, Lumsden A (2010). Building a bridal chamber: development of the thalamus. Trends Neurosci, 33(8), 373-380. Abstract.  Author URL.
Blackshaw S, Scholpp S, Placzek M, Ingraham H, Simerly R, Shimogori T (2010). Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation. J Neurosci, 30(45), 14925-14930. Abstract.  Author URL.
Peukert D, Scholpp S (2010). Ontogenesis of the brain: the development of the thalamus - the gateway to consciousness. BioSpektrum, 16(6), 639-643. Abstract.
Hagemann AI, Schindler S, Scholpp S (2010). The clathrin adaptor-protein subunit ap2m1 regulates canonical Wnt signalling in early neural development of the zebrafish.  Author URL.
Fassier C, Hutt JA, Scholpp S, Lumsden A, Giros B, Nothias F, Schneider-Maunoury S, Houart C, Hazan J (2010). Zebrafish atlastin controls motility and spinal motor axon architecture via inhibition of the BMP pathway. Nat Neurosci, 13(11), 1380-1387. Abstract.  Author URL.

2009

Yu SR, Burkhardt M, Nowak M, Ries J, Petrásek Z, Scholpp S, Schwille P, Brand M (2009). Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules. Nature, 461(7263), 533-536. Abstract.  Author URL.
Scholpp S, Delogu A, Gilthorpe J, Peukert D, Schindler S, Lumsden A (2009). Her6 regulates the neurogenetic gradient and neuronal identity in the thalamus. Proc Natl Acad Sci U S A, 106(47), 19895-19900. Abstract.  Author URL.
Scholpp S, Brand M (2009). Neural Patterning: Midbrain-Hindbrain Boundary. In  (Ed) Encyclopedia of Neuroscience, 205-211.  Abstract.

2007

Wendl T, Adzic D, Schoenebeck JJ, Scholpp S, Brand M, Yelon D, Rohr KB (2007). Early developmental specification of the thyroid gland depends on han-expressing surrounding tissue and on FGF signals. Development, 134(15), 2871-2879. Abstract.  Author URL.
Scholpp S, Foucher I, Staudt N, Peukert D, Lumsden A, Houart C (2007). Otx1l, Otx2 and Irx1b establish and position the ZLI in the diencephalon. Development, 134(17), 3167-3176. Abstract.  Author URL.
Erickson T, Scholpp S, Brand M, Moens CB, Waskiewicz AJ (2007). Pbx proteins cooperate with Engrailed to pattern the midbrain-hindbrain and diencephalic-mesencephalic boundaries. Dev Biol, 301(2), 504-517. Abstract.  Author URL.

2006

Scholpp S, Wolf O, Brand M, Lumsden A (2006). Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon. Development, 133(5), 855-864. Abstract.  Author URL.

2004

Scholpp S, Brand M (2004). Endocytosis controls spreading and effective signaling range of Fgf8 protein. Curr Biol, 14(20), 1834-1841. Abstract.  Author URL.
Heinze KG, Schlopp S, Brand M, Schwille P (2004). Probing intercellular pathways and propagation of Fgf8 signalling protein during embryogenesis by FCS.  Author URL.
Scholpp S, Groth C, Lohs C, Lardelli M, Brand M (2004). Zebrafish fgfr1 is a member of the fgf8 synexpression group and is required for fgf8 signalling at the midbrain-hindbrain boundary. Dev Genes Evol, 214(6), 285-295. Abstract.  Author URL.

2003

Scholpp S, Brand M (2003). Endocytosis controls propagation of Fgf8 during zebrafish embryogenesis.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon (vol 130, pg 4881, 2003). DEVELOPMENT, 130(21), 5293-5293. Author URL.
Scholpp S, Lohs C, Brand M (2003). Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon. Development, 130(20), 4881-4893. Abstract.  Author URL.
Scholpp S, Lohs C, Brand M (2003). Erratum: Engrailed and Fgf8 act synergistically to maintain the boundary between diencephalon and mesencephalon (Development vol. 130 (4881-4893)). Development, 130(21).
Scholpp S, Brand M (2003). Integrity of the midbrain region is required to maintain the diencephalic-mesencephalic boundary in zebrafish no isthmus/pax2.1 mutants. Dev Dyn, 228(3), 313-322. Abstract.  Author URL.

2002

Picker A, Scholpp S, Böhli H, Takeda H, Brand M (2002). A novel positive transcriptional feedback loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines. Development, 129(13), 3227-3239. Abstract.  Author URL.

2001

Scholpp S, Brand M (2001). Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain--hindbrain boundary development. Genesis, 30(3), 129-133. Author URL.

Steffen_Scholpp Details from cache as at 2019-11-20 05:26:25

Refresh publications

External Engagement and Impact

Administrative responsibilities

  • Deputy Director of Postgraduate Research (D-DPGR)
  • Organiser of the Biosciences Seminar Series

Committee/panel activities

  • Member of the reviewer panel for the OPUS, National Science Centre, NSC (2019)
  • Reviewer for the Royal Society  (2018 - present)
  • Reviewer for the Medical Research Council, MRC  (2017 - present)
  • Reviewer for Biotechnology and Biological Sciences Research Council (BBSRC) (2016 - present)
  • Reviewer for the National Science Centre, NSC Poland (2014 - present)
  • Reviewer for the German Research Council (DFG) Germany (2010 - present)
  • Scientific reviewer for DFG-Center for Regenerative Therapies, Dresden (CRTD) (2010)

Editorial responsibilities

  • Scientific editor of Mechanisms of Development (MOD) (2016 - present)
  • Scientific editor of Genesis, John Wiley and Sons, Inc (2015 - present)
  • Scientific editor of Molecular Science, AIMS Press (2014 - present)
  • Associated editor of Frontiers in Neuroscience (2011 - present)

External doctoral examining nationally and internationally

  • PhD       iBV, Nice U, FR          Supervisor Dr M. Fürthauer
  • PhD       Oxford Brookes U.     Supervisor Prof Alistair McGregor
  • MPhil     University of Bristol    Supervisor Dr Beck Richardson 2018

Internal doctoral examining responsibilities

  • PhD      UoE                             Supervisor Prof Michael Schrader 2018
  • PhD      UoE                             Supervisor Dr Tetsu Kudoh 2018     
  • MPhil    UoE                             Supervisor  Dr Matt Winter 2017

External positions

Selected activities as referee for scientific journals

eLife; Nature Communications; PLoS Biology; PNAS; EMBO reports; J Cell Sci; Development; Stem Cell Reports; Scientific Reports; Developmental Biology; Brain Structure & Function; Neurobiology of Disease; Neural Development; PLoS One; Differentiation; J of Medical Genetics; Cell & Tissue Research; Genesis; Cellular and Molecular Life Science; Development, Genes & Evolution

KIT

2016-2017       elected member of the KIT convent

2013 - 2014     Coordinator of the research cluster “Neural development and neural stem cells” of the Helmholtz Research Program “BioInterfaces”

2012 - 2013     Spokesman of the Young Investigator Network (YIN); assembly of junior faculties at

2010 - 2016    Member of the PhD Selection Committee for the BioInterfaces Graduate School, KIT

since 2010      Post-Graduate Student Advisor, KIT

2009 - 2011     Organizer of the Internal Seminar Series “On Fish and Technologies”, ITG, KIT

2009 - 2016     Member of the Faculty of Chemistry and Life Science, KIT, Germany


Invited lectures

Number of invited lectures in total

International lectures

National lectures

51

32

19

Recently invited lectures

2019

  • U Heidelberg
  • SignaLIFE, Nice
  • UCL
  • 8th Strategic Conference of Zebrafish Investigators, Asilomar, San Francisco, US

2018

  • NUS Singapore
  • University of Warsaw, Poland
  • University of Aberdeen
  • University of Bristol
  • University of Warwick
  • University of Manchester
  • University of Sheffield.

2017

  • Duke-NUS, Singapore
  • University of Calgary, Canada
  • ARUK Oxford
  • University of Madrid, Spain

2016

  • University of Exeter UK
  • University of Bath, UK
  • EZPM Lisbon, Portugal
  • EMBO Wnt meeting, Brno, Czech Republic

2015

  • UCSF, San Francisco, USA
  • Uni Jena, Germany
  • Dev. Biol. Soc. Meeting, Nuremberg, Germany
  • ETH Zürich Switzerland
  • EMBO workshop, Madrid, Spain
  • Gordon Research Conference on Developmental Biology, Mount Holyoke College, USA
  • DanStem Center, Copenhagen
  • Instituto de Neurociencias, Alicante, Spain.

Research networks

2019 -              Fellow of the Royal Society of Biology

2011 – 2015    Member of the DFG Research Network 1036: “Mechanism, functions and evolution of Wnt signalling pathways”

2007                Honorary Member of the Royal Microscopical Society (RMS)

 


Workshops/Conferences organised

2015                Session Chair, EMBO workshop „Signaling synapsis“, Madrid, Spain.

2014                Session Chair, European Zebrafish PI Meeting, Ein Gedi, Israel.

2013                Organizer, EMBO practical course „Imaging of Neural Development“, KIT, Germany.

                        Lecturer, Summer School, Jap Soc Dev Biol, Tokyo, Japan.

2012                Lecturer, GfE Summer School, Schloss Reissenburg, Ulm, Germany.

                        Session Chair, SURF Meeting Biozentrum Basel, Switzerland.

2011                Session Chair, Regional Meeting on Fish Genetics and Development, Landeck, Germany.

2002                Instructor, EMBO Developmental Biology Practical Course, MPI Tübingen, Germany.

Happy Lab Citizen

Teaching

BIO2088    Advanced Cell Biology (Module coordinator)

BIOM528   Advanced Topics in Biological Sciences

Academic tutor for yr1-yr3

Erasmus programme

 

Former Teaching Activities

2013 - 2016      Lecturer of the International Zebrafish and Medaka course (IZMC) at the EZRC

2010 - 2016      Lecturer of Biointerface International Graduate School (BIF-IGS)

2010 - 2016      Lecturer and supervisor in BSc and MSc program “Life Science” at the Faculty of Chemistry and Life Science; 1 lecture series per semester, supervision for 2 practical courses per semester

The Scholpp lab in 2017

Supervision / Group

Postdoctoral researchers

  • Lucy Brunt
  • Yosuke Ono
  • Sally Rogers
  • Joana Viana

Postgraduate researchers

  • Michael Dawes BBSRC DTP Student
  • Joshua Donnelly
  • Daniel Routledge MRC DTP Student
  • Chengting Zhang Chinese Scholarship Council (CSC) Student

Research Technicians

  • Holly Elson Research technician

Alumni

  • Bernadett Boesze PhD student (2012-2016)
  • Jenna Corcoran Research Tech (2017-2018)
  • Simone Geyer PhD student(2012-2015)
  • Anja Hagemann PostDoc (2009-2014)
  • Benjamin Mattes PhD student (2015-2018)
  • Daniela Peukert PhD student(2008-2011)
  • Lauren Porter MSc student(2018-2019)
  • Charanya Rengarajan PhD student (2009-2013)
  • Simone Schindler Technician (2017-2018)
  • Eliana Stanganello PhD student, summa cum laude (2011-2015)

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