AbstractThe lack of commercially available low-cost laboratory spraying equipment for testing microbial control agents can hinder advancement in the field of biocontrol. This study presents an inexpensive, portable sprayer that is calibrated utilizing laboratory consumables. The computer aided design files are made available so that it is freely modifiable and can be used for machine routing or 3D printing. Bioassay data was obtained by spraying Myzus persicae with spores of entomopathogenic fungi. Observed variation in droplet deposition within tested pressure and volume settings, and spore deposition within sprayed concentrations were low. Bioassay results show reproducible mortality for the tested doses.

Understanding the ecological and genetic factors that determine the evolution of virulence has broad value for invertebrate pathology. In addition to helping us understand the fundamental biology of our study organisms this body of theory has important applications in microbial biocontrol. Experimental tests of virulence theory are often carried out in invertebrate models and yet theory rarely informs applied passage experiments that aim to increase or maintain virulence. This review summarizes recent progress in this field with a focus on work most relevant to biological control: the virulence of invertebrate pathogens that are 'obligate killers' and which require cadavers for the production of infectious propagules. We discuss recent theory and fundamental and applied experimental evolution with bacteria, fungi, baculoviruses and nematodes. While passage experiments using baculoviruses have a long history of producing isolates with increased virulence, studies with other pathogens have not been so successful. Recent passage experiments that have applied evolution of virulence frameworks based on cooperation (kin selection) have produced novel methods and promising mutants with increased killing power. Evolution of virulence theory can provide plausible explanations for the varied results of passage experiments as well as a predictive framework for improving artificial selection.

BACKGROUND: Biological control is a cornerstone of integrated pest management and could also play a key role in managing the evolution of insecticide resistance. Ecological theory predicts that the fitness cost of insecticide resistance can be increased under exposure to invertebrate natural enemies or pathogens, and can therefore increase the value of integrating biological control into pest management. In this study of the peach potato aphid, Myzus persicae, we aimed to identify whether insecticide resistance affected fitness and vulnerability of different aphid clones to the entomopathogenic fungus Akanthomyces muscarius. RESULTS: Insecticide resistant clones were found to be slightly less susceptible to the pathogen than susceptible clones. However, this pattern could also be explained by the influence of length of laboratory culture, which was longer in susceptible clones and was positively correlated with susceptibility to fungi. Furthermore, resistance status did not affect aphid development time or intrinsic rate of increase of aphids. Finally, in a cage trial the application of fungus did not increase the competitive fitness of insecticide resistant clone 'O'. CONCLUSION: We found no fitness cost in reproductive rate or pathogen susceptibility associated with chemical resistance in M. persicae. In contrast, some susceptible clones, particularly those subject to decades of laboratory rearing, showed enhanced susceptibility to a fungal pathogen, but not reduced reproductive fitness, an observation consistent with down-regulation of costly immune functions in culture. Overall, fungal pathogen control is compatible with insecticides and should not increase the selection pressure for resistance of M. persicae to chemical insecticides.