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Dr Andrea Dowling

Dr Andrea Dowling

Research Fellow

 +44(0)1326 371872

 Daphne du Maurier 

 

Daphne du Maurier Building, University of Exeter,  Penryn Campus, Penryn, Cornwall, TR10 9FE, UK

Overview

Qualifications

1999: BSc Hons Natural Sciences, University of Bath
2000: MSc Crop Protection, University of Bristol
2005: PhD Investigation into the Mode of Action of Mcf1 Toxin, University of Bath

Research

Research interests

Photorhabdus toxins

Photorhabdus are Gram-negative, nematode associated entomopathogenic bacteria, which secrete a wide range of toxins and virulence factors swamping host defences and killing target insect larvae.

My current research focuses on one of these toxins - a novel pro-apoptotic protein, Makes Caterpillars Floppy 1 (Mcf1), which is active against both insect and mammalian cells. I am researching interactions of this toxin with the host cell itself, the cell death machinery and biochemical pathways/ cellular components in a bid to elucidate the mode of action of this toxin.  Providing a toolbox for evaluating bacterial toxin mode of action.

Research networks

Prof. Charles Streuli, University of Manchester
Prof. Richard Titball, University of Exeter
Dr Barbara Reaves, University of Bath
Dr Nick Waterfield, University of Bath
Dr Jean van den Elsen, University of Bath
Dr Rob Jackson, University of Reading

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Dowling A, Hill G, Bonneaud C (In Press). Multiple differences in pathogen-host cell interactions following a bacterial host shift. Scientific Reports Full text.
Dowling AJ (2012). Novel gain of function approaches for vaccine candidate identification in Burkholderia pseudomallei. Front Cell Infect Microbiol, 2 Abstract.  Author URL.
Proschak A, Schultz K, Herrmann J, Dowling AJ, Brachmann AO, Ffrench-Constant R, Müller R, Bode HB (2011). Cytotoxic fatty acid amides from Xenorhabdus. ChemBioChem, 12(13), 2011-2015.
Eleftherianos I, ffrench-Constant RH, Clarke DJ, Dowling AJ, Reynolds SE (2010). Dissecting the immune response to the entomopathogen Photorhabdus. Trends in Microbiology, 18(12), 552-560. Abstract.
Dowling AJ, Wilkinson PA, Holden MTG, Quail MA, Bentley SD, Reger J, Waterfield NR, Titball RW, Ffrench-Constant RH (2010). Genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen Burkholderia pseudomallei K96243. PLoS One, 5(12). Abstract.  Author URL.  Full text.
Poulter N, Munoz-Berbel X, Johnson AL, Dowling AJ, Waterfield N, Jenkins ATA (2009). An organo-silver compound that shows antimicrobial activity against Pseudomonas aeruginosa as a monomer and plasma deposited film. Chemical Communications(47), 7312-7314. Abstract.
Vlisidou I, Dowling AJ, Evans IR, Waterfield N, ffrench-Constant RH, Wood W (2009). Drosophila embryos as model systems for monitoring bacterial infection in real time. PLoS Pathog, 5(7). Abstract.  Author URL.  Full text.
Waterfield NR, Sanchez-Contreras M, Eleftherianos I, Dowling A, Yang G, Wilkinson P, Parkhill J, Thomson N, Reynolds SE, Bode HB, et al (2008). Rapid Virulence Annotation (RVA): identification of virulence factors using a bacterial genome library and multiple invertebrate hosts. Proc Natl Acad Sci U S A, 105(41), 15967-15972. Abstract.  Author URL.
Hares MC, Hinchliffe SJ, Strong PCR, Eleftherianos I, Dowling AJ, Ffrench-Constant RH, Waterfield N (2008). The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is active against cultured mammalian cells. Microbiology, 154(Pt 11), 3503-3517. Abstract.  Author URL.
ffrench-Constant RH, Dowling A, Waterfield NR (2007). Insecticidal toxins from Photorhabdus bacteria and their potential use in agriculture. Toxicon, 49(4), 436-451. Abstract.  Author URL.
Dowling AJ, Waterfield NR, Hares MC, Le Goff G, Streuli CH, ffrench-Constant RH (2007). The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain. Cell Microbiol, 9(10), 2470-2484. Abstract.  Author URL.
Dowling AJ, Daborn PJ, Waterfield NR, Wang P, Streuli CH, ffrench-Constant RH (2004). The insecticidal toxin Makes caterpillars floppy (Mcf) promotes apoptosis in mammalian cells. CELLULAR MICROBIOLOGY, 6(4), 345-353. Author URL.
Waterfield NR, Daborn PJ, Dowling AJ, Yang G, Hares M, ffrench-Constant RH (2003). The insecticidal toxin makes caterpillars floppy 2 (Mcf2) shows similarity to HrmA, an avirulence protein from a plant pathogen. FEMS Microbiol Lett, 229(2), 265-270. Abstract.  Author URL.
Waterfield N, Dowling A, Sharma S, Daborn PJ, Potter U, Ffrench-Constant RH (2001). Oral toxicity of Photorhabdus luminescens W14 toxin complexes in Escherichia coli. Appl Environ Microbiol, 67(11), 5017-5024. Abstract.  Author URL.

Publications by year


In Press

Dowling A, Hill G, Bonneaud C (In Press). Multiple differences in pathogen-host cell interactions following a bacterial host shift. Scientific Reports Full text.

2012

Dowling AJ (2012). Novel gain of function approaches for vaccine candidate identification in Burkholderia pseudomallei. Front Cell Infect Microbiol, 2 Abstract.  Author URL.

2011

Proschak A, Schultz K, Herrmann J, Dowling AJ, Brachmann AO, Ffrench-Constant R, Müller R, Bode HB (2011). Cytotoxic fatty acid amides from Xenorhabdus. ChemBioChem, 12(13), 2011-2015.

2010

Eleftherianos I, ffrench-Constant RH, Clarke DJ, Dowling AJ, Reynolds SE (2010). Dissecting the immune response to the entomopathogen Photorhabdus. Trends in Microbiology, 18(12), 552-560. Abstract.
Dowling AJ, Wilkinson PA, Holden MTG, Quail MA, Bentley SD, Reger J, Waterfield NR, Titball RW, Ffrench-Constant RH (2010). Genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen Burkholderia pseudomallei K96243. PLoS One, 5(12). Abstract.  Author URL.  Full text.

2009

Poulter N, Munoz-Berbel X, Johnson AL, Dowling AJ, Waterfield N, Jenkins ATA (2009). An organo-silver compound that shows antimicrobial activity against Pseudomonas aeruginosa as a monomer and plasma deposited film. Chemical Communications(47), 7312-7314. Abstract.
Vlisidou I, Dowling AJ, Evans IR, Waterfield N, ffrench-Constant RH, Wood W (2009). Drosophila embryos as model systems for monitoring bacterial infection in real time. PLoS Pathog, 5(7). Abstract.  Author URL.  Full text.

2008

Waterfield NR, Sanchez-Contreras M, Eleftherianos I, Dowling A, Yang G, Wilkinson P, Parkhill J, Thomson N, Reynolds SE, Bode HB, et al (2008). Rapid Virulence Annotation (RVA): identification of virulence factors using a bacterial genome library and multiple invertebrate hosts. Proc Natl Acad Sci U S A, 105(41), 15967-15972. Abstract.  Author URL.
Hares MC, Hinchliffe SJ, Strong PCR, Eleftherianos I, Dowling AJ, Ffrench-Constant RH, Waterfield N (2008). The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is active against cultured mammalian cells. Microbiology, 154(Pt 11), 3503-3517. Abstract.  Author URL.

2007

ffrench-Constant RH, Dowling A, Waterfield NR (2007). Insecticidal toxins from Photorhabdus bacteria and their potential use in agriculture. Toxicon, 49(4), 436-451. Abstract.  Author URL.
Dowling AJ, Waterfield NR, Hares MC, Le Goff G, Streuli CH, ffrench-Constant RH (2007). The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain. Cell Microbiol, 9(10), 2470-2484. Abstract.  Author URL.

2004

Dowling AJ, Daborn PJ, Waterfield NR, Wang P, Streuli CH, ffrench-Constant RH (2004). The insecticidal toxin Makes caterpillars floppy (Mcf) promotes apoptosis in mammalian cells. CELLULAR MICROBIOLOGY, 6(4), 345-353. Author URL.

2003

Waterfield NR, Daborn PJ, Dowling AJ, Yang G, Hares M, ffrench-Constant RH (2003). The insecticidal toxin makes caterpillars floppy 2 (Mcf2) shows similarity to HrmA, an avirulence protein from a plant pathogen. FEMS Microbiol Lett, 229(2), 265-270. Abstract.  Author URL.

2001

Waterfield N, Dowling A, Sharma S, Daborn PJ, Potter U, Ffrench-Constant RH (2001). Oral toxicity of Photorhabdus luminescens W14 toxin complexes in Escherichia coli. Appl Environ Microbiol, 67(11), 5017-5024. Abstract.  Author URL.

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