Dr Michail Isupov
Postdoctoral researcher
M.Isupov@exeter.ac.uk
3451
Geoffrey Pope
Geoffrey Pope Building, University of Exeter , Stocker Road, Exeter, EX4 4QD, UK
Publications
Journal articles
Gambelli L, McLaren M, Conners R, Sanders K, Gaines MC, Clark L, Gold VAM, Kattnig D, Sikora M, Hanus C, et al (2024). Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius. eLife, 13 Abstract.
McLaren M, Conners R, Isupov MN, Gil-Díez P, Gambelli L, Gold VAM, Walter A, Connell SR, Williams B, Daum B, et al (2023). CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state. Nature Microbiology, 8(10), 1834-1845. Abstract.
De Rose SA, Isupov MN, Worthy HL, Stracke C, Harmer NJ, Siebers B, Littlechild JA, consortium TH, Siebers B, Bräsen C, et al (2023). Structural characterization of a novel cyclic 2,3-diphosphoglycerate synthetase involved in extremolyte production in the archaeon Methanothermus fervidus. Frontiers in Microbiology, 14
Freda I, Exertier C, Barile A, Chaves-Sanjuan A, Vega MV, Isupov MN, Harmer NJ, Gugole E, Swuec P, Bolognesi M, et al (2023). Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR. Nucleic Acids Research, 51(15), 8237-8254. Abstract.
Banerjee S, Varga JK, Kumar M, Zoltsman G, Rotem‐Bamberger S, Cohen‐Kfir E, Isupov MN, Rosenzweig R, Schueler‐Furman O, Wiener R, et al (2023). Structural study of <scp>UFL1‐UFC1</scp> interaction uncovers the role of <scp>UFL1</scp> N‐terminal helix in ufmylation. EMBO reports, 24(12). Abstract.
Khazma T, Grossman A, Guez-Haddad J, Feng C, Dabas H, Sain R, Weitman M, Zalk R, Isupov MN, Hammarlund M, et al (2023). Structure-function analysis of ceTIR-1/hSARM1 explains the lack of Wallerian axonal degeneration in C. elegans. Cell Rep, 42(9). Abstract. Author URL.
Roy S, Vivoli Vega M, Ames JR, Britten N, Kent A, Evans K, Isupov MN, Harmer NJ (2023). The ROK kinase N-acetylglucosamine kinase uses a sequential random enzyme mechanism with successive conformational changes upon each substrate binding. Journal of Biological Chemistry, 299(4), 103033-103033.
Gambelli L, Isupov MN, Conners R, McLaren M, Bellack A, Gold V, Rachel R, Daum B (2022). An archaellum filament composed of two alternating subunits. Nature Communications, 13(1). Abstract.
Krissinel E, Lebedev AA, Uski V, Ballard CB, Keegan RM, Kovalevskiy O, Nicholls RA, Pannu NS, Skubák P, Berrisford J, et al (2022). CCP4 Cloud for structure determination and project management in macromolecular crystallography. Acta Crystallogr D Struct Biol, 78(Pt 9), 1079-1089. Abstract. Author URL.
Gaines MC, Isupov MN, Sivabalasarma S, Haque RU, McLaren M, Mollat CL, Tripp P, Neuhaus A, Gold VAM, Albers S-V, et al (2022). Electron cryo-microscopy reveals the structure of the archaeal thread filament. Nature Communications, 13(1). Abstract.
Gambelli L, Isupov MN, Daum B (2022). Escaping the symmetry trap in helical reconstruction. Faraday Discussions, 240, 303-311. Abstract.
Cross AR, Roy S, Vivoli Vega M, Rejzek M, Nepogodiev SA, Cliff M, Salmon D, Isupov MN, Field RA, Prior JL, et al (2022). Spinning sugars in antigen biosynthesis: characterization of the Coxiella burnetii and Streptomyces griseus TDP-sugar epimerases. J Biol Chem, 298(5). Abstract. Author URL.
Sutter J-M, Mitchell DE, Schmidt M, Isupov MN, Littlechild JA, Schönheit P (2022). Substrate specificity of branched chain amino acid aminotransferases: the substitution of glycine to serine in the active site determines the substrate specificity for α-ketoglutarate. Frontiers in Catalysis, 2
De Rose SA, Kuprat T, Isupov MN, Reinhardt A, Schönheit P, Littlechild JA (2021). Biochemical and Structural Characterisation of a Novel D-Lyxose Isomerase from the Hyperthermophilic Archaeon Thermofilum sp. Frontiers in Bioengineering and Biotechnology, 9 Abstract.
Gambelli L, Isupov MN, Conners R, McLaren M, Bellack A, Gold V, Rachel R, Daum B (2021). New insights into the architecture and dynamics of archaella. Abstract.
Cross AR, Roy S, Vega MV, Rejzek M, Nepogodiev SA, Cliff M, Salmon D, Isupov MN, Field RA, Prior JL, et al (2021). Spinning sugars in antigen biosynthesis: a direct study of the <i>Coxiella burnetii</i> and <i>Streptomyces griseus</i> TDP-sugar epimerases. Abstract.
Zhu C, Chen Y, Isupov MN, Littlechild JA, Sun L, Liu X, Wang Q, Gong H, Dong P, Zhang N, et al (2021). Structural Insights into a Novel Esterase from the East Pacific Rise and its Improved Thermostability by a Semirational Design. Journal of Agricultural and Food Chemistry, 69(3), 1079-1090.
Kumar M, Padala P, Fahoum J, Hassouna F, Tsaban T, Zoltsman G, Banerjee S, Cohen-Kfir E, Dessau M, Rosenzweig R, et al (2021). Structural basis for UFM1 transfer from UBA5 to UFC1. Nature Communications, 12(1). Abstract.
Roy S, Vega MV, Ames JR, Britten N, Kent A, Evans K, Isupov MN, Harmer NJ (2021). Structure and function of <i>N</i>-acetylglucosamine kinase illuminates the catalytic mechanism of ROK kinases. Abstract.
James P, Isupov MN, De Rose SA, Sayer C, Cole IS, Littlechild JA (2020). A ‘Split-Gene’ Transketolase from the Hyper-Thermophilic Bacterium Carboxydothermus hydrogenoformans: Structure and Biochemical Characterization. Frontiers in Microbiology, 11
Sporny M, Guez-Haddad J, Khazma T, Yaron A, Dessau M, Shkolnisky Y, Mim C, Isupov MN, Zalk R, Hons M, et al (2020). Structural basis for SARM1 inhibition and activation under energetic stress. eLife, 9 Abstract.
Yilmazer B, Isupov MN, De Rose SA, Bulut H, Benninghoff JC, Binay B, Littlechild JA (2020). Structural insights into the NAD+-dependent formate dehydrogenase mechanism revealed from the NADH complex and the formate NAD+ ternary complex of the Chaetomium thermophilum enzyme. Journal of Structural Biology, 212(3), 107657-107657.
Wang Q, Jiang M, Isupov MN, Chen Y, Littlechild JA, Sun L, Wu X, Wang Q, Yang W, Chen L, et al (2020). The crystal structure of Arabidopsis BON1 provides insights into the copine protein family. Plant J, 103(3), 1215-1232. Abstract. Author URL.
Cutlan R, De Rose S, Isupov MN, Littlechild JA, Harmer NJ (2020). Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1868(2), 140322-140322.
Soudah N, Padala P, Hassouna F, Kumar M, Mashahreh B, Lebedev AA, Isupov MN, Cohen-Kfir E, Wiener R (2019). An N-Terminal Extension to UBA5 Adenylation Domain Boosts UFM1 Activation: Isoform-Specific Differences in Ubiquitin-like Protein Activation. J Mol Biol, 431(3), 463-478. Abstract. Author URL.
Sporny M, Guez-Haddad J, Lebendiker M, Ulisse V, Volf A, Mim C, Isupov MN, Opatowsky Y (2019). Structural Evidence for an Octameric Ring Arrangement of SARM1. J Mol Biol, 431(19), 3591-3605. Abstract. Author URL.
Barak R, Yom-Tov G, Guez-Haddad J, Gasri-Plotnitsky L, Maimon R, Cohen-Berkman M, McCarthy AA, Perlson E, Henis-Korenblit S, Isupov MN, et al (2019). Structural Principles in Robo Activation and Auto-inhibition. CELL, 177(2), 272-+. Author URL.
Jiang M, Sun L, Isupov MN, Littlechild JA, Wu X, Wang Q, Wang Q, Yang W, Wu Y (2019). Structural basis for the Target DNA recognition and binding by the MYB domain of phosphate starvation response 1. FEBS J, 286(14), 2809-2821. Abstract. Author URL.
Biterova EI, Isupov MN, Keegan RM, Lebedev AA, Sohail AA, Liaqat I, Alanen HI, Ruddock LW (2019). The crystal structure of human microsomal triglyceride transfer protein. Proc Natl Acad Sci U S A, 116(35), 17251-17260. Abstract. Author URL.
Isupov MN, Boyko KM, Sutter J-M, James P, Sayer C, Schmidt M, Schonheit P, Nikolaeva AY, Stekhanova TN, Mardanov AV, et al (2019). Thermostable Branched-Chain Amino Acid Transaminases from the Archaea <i>Geoglobus acetivorans</i> and <i>Archaeoglobus fulgidus</i>: Biochemical and Structural Characterization (vol 7, 7, 2019). FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 7 Author URL.
Isupov MN, James P, Littlechild JA (2019). Thermostable Branched-Chain Amino Acid Transaminases from the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus: Biochemical and Structural Characterization. Front. Bioeng. Biotechnol, 7, 7-7.
Line K, Isupov MN, LaCourse EJ, Cutress DJ, Morphew RM, Brophy PM, Littlechild JA (2019). X-ray structure of Fasciola hepatica Sigma class glutathione transferase 1 reveals a disulfide bond to support stability in gastro-intestinal environment. Scientific Reports, 9(1). Abstract.
Knight AR, Taylor EL, Lukaszewski R, Jensen KT, Jones HE, Carre JE, Isupov MN, Littlechild JA, Bailey SJ, Brewer E, et al (2018). A high-sensitivity electrochemiluminescence-based ELISA for the measurement of the oxidative stress biomarker, 3-nitrotyrosine, in human blood serum and cells. FREE RADICAL BIOLOGY AND MEDICINE, 120, 246-254. Author URL.
Ferrandi EE, Sayer C, De Rose SA, Guazzelli E, Marchesi C, Saneei V, Isupov MN, Littlechild JA, Monti D (2018). New Thermophilic α/β Class Epoxide Hydrolases Found in Metagenomes from Hot Environments. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 6 Author URL.
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, et al (2018). The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. JOURNAL OF BIOLOGICAL CHEMISTRY, 293(50), 19429-19440. Author URL.
Isupov MN, Schröder E, Gibson RP, Beecher J, Donadio G, Saneei V, Dcunha SA, McGhie EJ, Sayer C, Davenport CF, et al (2018). The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of a type II Baeyer-Villiger monooxygenase. Corrigendum. Acta Crystallogr D Struct Biol, 74(Pt 4). Abstract. Author URL.
Vogeler S, Galloway TS, Isupov M, Bean TP (2017). Cloning retinoid and peroxisome proliferator-activated nuclear receptors of the Pacific oyster and in silico binding to environmental chemicals. PLoS One, 12(4). Abstract. Author URL.
Littlechild JA, Isupov MN (2017). Comments to Article by Willetts A. et al. Microorganisms 2016, 4, 38. Microorganisms, 5(3). Abstract. Author URL.
Zhang J, Fan F, Zhao Y, Sun L, Liu Y, Keegan RM, Isupov MN, Wu Y (2017). Crystal structure of the type IV secretion system component CagX from <i>Helicobacter pylori</i>. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 73, 167-173. Author URL.
Romano-Moreno M, Rojas AL, Williamson CD, Gershlick DC, Lucas M, Isupov MN, Bonifacino JS, Machner MP, Hierro A (2017). Molecular mechanism for the subversion of the retromer coat by the <i>Legionella</i> effector RidL. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(52), E11151-E11160. Author URL.
Sporny M, Guez-Haddad J, Kreusch A, Shakartzi S, Neznansky A, Cross A, Isupov MN, Qualmann B, Kessels MM, Opatowsky Y, et al (2017). Structural History of Human SRGAP2 Proteins. MOLECULAR BIOLOGY AND EVOLUTION, 34(6), 1463-1478. Author URL.
Padala P, Soudah N, Giladi M, Haitin Y, Isupov MN, Wiener R (2017). The Crystal Structure and Conformations of an Unbranched Mixed Tri-Ubiquitin Chain Containing K48 and K63 Linkages. JOURNAL OF MOLECULAR BIOLOGY, 429(24), 3801-3813. Author URL.
Vivoli M, Isupov MN, Nicholas R, Hill A, Scott A, Kosma P, Prior J, Harmer NJ (2016). Burkholderia pseudomallei heptokinase WcbL, D-mannose complex.
Zarafeta D, Kissas D, Sayer C, Gudbergsdottir SR, Ladoukakis E, Isupov MN, Chatziioannou A, Peng X, Littlechild JA, Skretas G, et al (2016). Discovery and Characterization of a Thermostable and Highly Halotolerant GH5 Cellulase from an Icelandic Hot Spring Isolate. PLoS One, 11(1). Abstract. Author URL.
Sporny M, Guez-Haddad J, Waterman DG, Isupov MN, Opatowsky Y (2016). Molecular symmetry-constrained systematic search approach to structure solution of the coiled-coil SRGAP2 F-BARx domain. Acta Crystallographica Section D Structural Biology, 72(12), 1241-1253. Abstract.
sayer C, Finnigan W, Isupov MN, Levisson M, Kengen SWM, van der Oost J, Harmer NJ, Littlechild JA (2016). Structural and biochemical characterisation of Archaeoglobus fulgidus esterase reveals a bound CoA molecule in the vicinity of the active site. Scientific Reports, 6, 25542-25542. Abstract.
Ferrandi EE, Sayer C, Isupov MN, Annovazzi C, Marchesi C, Iacobone G, Peng X, Bonch-Osmolovskaya E, Wohlgemuth R, Littlechild JA, et al (2015). Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries. FEBS J, 282(15), 2879-2894. Abstract. Author URL.
Novak HR, Sayer C, Isupov MN, Littlechild JA (2015). Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase.
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from Thermogutta terrifontis (L37A mutant with butyrate bound).
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native).
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from Thermogutta terrifontis (acetate bound).
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from Thermogutta terrifontis (cacodylate complex).
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from Thermogutta terrifontis (malate bound).
Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA (2015). Structural studies of a thermophilic esterase from a new Planctomycetes species, Thermogutta terrifontis. FEBS J, 282(15), 2846-2857. Abstract. Author URL.
Sayer C, Szabo Z, Isupov MN, Ingham C, Littlechild JA (2015). The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain. Front Microbiol, 6 Abstract. Author URL.
Isupov MN, Schroeder E, Gibson RP, Beecher J, Donadio G, Saneei V, Dcunha SA, McGhie EJ, Sayer C, Davenport CF, et al (2015). The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of <i>Pseudomonas putida</i>: the first crystal structure of a type II Baeyer-Villiger monooxygenase. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 71, 2344-2353. Author URL.
Vivoli M, Isupov MN, Nicholas R, Hill A, Scott AE, Kosma P, Prior JL, Harmer NJ (2015). Unraveling the B. pseudomallei Heptokinase WcbL: from Structure to Drug Discovery. Chemistry and Biology, 22(12), 1622-1632. Abstract.
Novak HR, Sayer C, Isupov MN, Gotz D, Spragg AM, Littlechild JA (2014). Biochemical and structural characterisation of a haloalkane dehalogenase from a marine Rhodobacteraceae. FEBS Lett, 588(9), 1616-1622. Abstract. Author URL.
Gibson RP, Isupov M, Littlechild JA (2014). Crystal Structure of the FMN-reductase Msue from Pseudomonas putida KT2440.
Novak HR, Sayer C, Isupov M, Gotz D, Spragg AM, Littlechild JA (2014). Haloalkane dehalogenase.
Novak HR, Sayer C, Isupov M, Gotz D, Spragg AM, Littlechild JA (2014). Haloalkane dehalogenase with 1-hexanol.
Novak HR, Sayer C, Isupov MN, Littlechild JA (2014). Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase.
Novak HR, Sayer C, Isupov MN, Littlechild JA (2014). Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase.
Novak HR, Sayer C, Isupov MN, Littlechild JA (2014). Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase.
Novak HR, Sayer C, Isupov MN, Littlechild JA (2014). Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase.
Lebedev AA, Isupov MN (2014). Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda. Acta Crystallographica Section D: Biological Crystallography, 70(9), 2430-2443. Abstract.
Vivoli M, Ayres E, Beaumont E, Isupov MN, Harmer NJ (2014). Structural insights into WcbI, a novel polysaccharide-biosynthesis enzyme. IUCrJ, 1, 28-38.
Littlechild J, Sayer C, Isupov M, Ward J, Littlechild J (2014). Structural studies on transaminase enzymes and applications in biocatalysis. NEW BIOTECHNOLOGY, 31, S19-S19. Author URL.
Sayer C, Isupov M, Martinez-Torres RJ, Richter N, Hailes HC, Ward J, Littlechild J (2014). The (R)-selective transaminase from Nectria haematococca.
James P, Isupov MN, Sayer C, Saneei V, Berg S, Lioliou M, Kotlar HK, Littlechild JA (2014). The structure of a tetrameric α-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability. Acta Crystallogr D Biol Crystallogr, 70(Pt 10), 2607-2618. Abstract. Author URL.
Sayer C, Martinez-Torres RJ, Richter N, Isupov MN, Hailes HC, Littlechild JA, Ward JM (2014). The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca. FEBS J, 281(9), 2240-2253. Abstract. Author URL.
Sayer C, Isupov MN, Littlechild JA (2013). Crystal structure of omega-transaminase from Chromobacterium violaceum.
Sayer C, Isupov MN, Littlechild JA (2013). Crystal structure of the apo omega-transaminase from Chromobacterium violaceum.
Reid EL, Weynberg KD, Love J, Isupov MN, Littlechild JA, Wilson WH, Kelly SL, Lamb DC, Allen MJ (2013). Functional and structural characterisation of a viral cytochrome b5. FEBS Lett, 587(22), 3633-3639. Abstract. Author URL.
Novak HR, Sayer C, Isupov MN, Paszkiewicz K, Gotz D, Spragg AM, Littlechild JA (2013). Marine Rhodobacteraceae L-haloacid dehalogenase contains a novel His/Glu dyad that could activate the catalytic water. FEBS J, 280(7), 1664-1680. Abstract. Author URL.
Novak HR, Sayer C, Isupov MN, Paszkiewicz K, Gotz D, Spragg AM, Littlechild JA (2013). Native L-haloacid dehalogenase from a Rhodobacteraceae family bacterium.
Isupov MN, Lebedev AA, Westlake A, Sayer C, Littlechild JA (2013). Pseudomonas aeruginosa beta-alanine:pyruvate aminotransferase holoenzyme without divalent cations on dimer-dimer interface.
Vivoli M, Ayres E, Isupov MN, Harmer NJ (2013). Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.
Sayer C, Isupov MN, Westlake A, Littlechild JA (2013). Structural studies of Pseudomonas and Chromobacterium ω- aminotransferases provide insights into their differing substrate specificity. Acta Crystallographica Section D: Biological Crystallography, 69(4), 564-576. Abstract.
Sayer C, Isupov MN, Westlake A, Littlechild JA (2013). The structure of the omega aminotransferase from Pseudomonas aeruginosa.
Sayer C, Isupov MN, Westlake A, Littlechild JA (2013). The structure of the omega aminotransferase from Pseudomonas aeruginosa.
Sayer C, Bommer M, Isupov M, Ward J, Littlechild J (2012). Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus. Acta Crystallogr D Biol Crystallogr, 68(Pt 7), 763-772. Abstract. Author URL.
Urusova DV, Isupov MN, Antonyuk S, Kachalova GS, Obmolova G, Vagin AA, Lebedev AA, Burenkov GP, Dauter Z, Bartunik HD, et al (2012). Crystal structure of D-serine dehydratase from Escherichia coli. Biochimica et Biophysica Acta - Proteins and Proteomics, 1824(3), 422-432. Abstract.
Lebedev AA, Young P, Isupov MN, Moroz OV, Vagin AA, Murshudov GN (2012). JLigand: a graphical tool for the CCP4 template-restraint library. Acta Crystallogr D Biol Crystallogr, 68(Pt 4), 431-440. Abstract. Author URL.
Shevtsov MB, Chen Y, Isupov MN, Leech A, Gollnick P, Antson AA (2010). Bacillus licheniformis Anti-TRAP can assemble into two types of dodecameric particles with the same symmetry but inverted orientation of trimers. Journal of Structural Biology, 170(1), 127-133. Abstract.
Opperman DJ, Sewell BT, Litthauer D, Isupov MN, Littlechild JA, van Heerden E (2010). Crystal structure of a thermostable old yellow enzyme from Thermus scotoductus SA-01. Biochem Biophys Res Commun, 393(3), 426-431. Abstract. Author URL.
Opperman DJ, Sewell BT, Litthauer D, Isupov MN, Littlechild JA, van Heerden E (2010). Old Yellow Enzyme from Thermus scotoductus SA-01.
Willies S, Isupov M, Littlechild J (2010). Thermophilic enzymes and their applications in biocatalysis: a robust aldo-keto reductase. Environ Technol, 31(10), 1159-1167. Abstract. Author URL.
Bourne PC, Isupov MN, Littlechild JA (2009). Alcaligenes esterase complexed with product analogue.
Rye CA, Isupov MN, Lebedev AA, Littlechild JA (2009). Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii. Extremophiles, 13(1), 179-190. Abstract. Author URL.
Ng CL, Waterman DG, Koonin EV, Walters AD, Chong JP, Isupov MN, Lebedev AA, Bunka DH, Stockley PG, Ortiz-Lombardía M, et al (2009). Conformational flexibility and molecular interactions of an archaeal homologue of the Shwachman-Bodian-Diamond syndrome protein. BMC Structural Biology, 9 Abstract.
Willies SC, Isupov MN, Garman EF, Littlechild JA (2009). The binding of haem and zinc in the 1.9 a X-ray structure of Escherichia coli bacterioferritin. J Biol Inorg Chem, 14(2), 201-207. Abstract. Author URL.
Littlechild J, Garcia Rodriguez E, Isupov M (2009). Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography. J Inorg Biochem, 103(4), 617-621. Abstract. Author URL.
Line K, Isupov MN, Garcia-Rodriguez E, Maggioli G, Parra F, Littlechild JA (2008). The Fasciola hepatica thioredoxin: High resolution structure reveals two oxidation states. Mol Biochem Parasitol, 161(1), 44-48. Abstract. Author URL.
Rye CA, Isupov MN, Lebedev AA, Littlechild JA (2007). An order-disorder twin crystal of L-2-haloacid dehalogenase from Sulfolobus tokodaii. Acta Crystallogr D Biol Crystallogr, 63(Pt 8), 926-930. Abstract. Author URL.
Sayer C, Isupov MN, Littlechild JA (2007). Crystallization and preliminary X-ray diffraction analysis of omega-amino acid:pyruvate transaminase from Chromobacterium violaceum. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63(Pt 2), 117-119. Abstract. Author URL.
Isupov MN, Lebedev AA (2007). NCS-constrained exhaustive search using oligomeric models. Acta Crystallographica Section D: Biological Crystallography, 64(1), 90-98. Abstract.
Littlechild JA, Guy J, Connelly S, Mallett L, Waddell S, Rye CA, Line K, Isupov M (2007). Natural methods of protein stabilization: thermostable biocatalysts. Biochem Soc Trans, 35(Pt 6), 1558-1563. Abstract. Author URL.
Sanders CM, Kovalevskiy OV, Sizov D, Lebedev AA, Isupov MN, Antson AA (2007). Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors. Nucleic Acids Research, 35(19), 6451-6457. Abstract.
Conklin PL, Gatzek S, Wheeler GL, Dowdle J, Raymond MJ, Rolinski S, Isupov M, Littlechild JA, Smirnoff N (2006). Arabidopsis thaliana VTC4 encodes L-galactose-1-P phosphatase, a plant ascorbic acid biosynthetic enzyme. J Biol Chem, 281(23), 15662-15670. Abstract. Author URL.
Littlechild J, Garcia-Rodriguez E, Isupov M (2006). INOR 398-Crystallization studies of vanadium haloperoxidases: Insights into halide specificity and enzyme mechanism. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 232 Author URL.
Connelly S, Line K, Isupov MN, Littlechild JA (2005). Synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the Aureobacterium species (-) gamma-lactamase. Org Biomol Chem, 3(18), 3260-3262. Abstract. Author URL.
Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB (2004). Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix. Journal of Molecular Biology, 343(3), 547-557. Abstract.
Isupov MN, Brindley AA, Hollingsworth EJ, Murshudov GN, Vagin AA, Littlechild JA (2004). Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. Acta Crystallogr D Biol Crystallogr, 60(Pt 10), 1879-1882. Abstract. Author URL.
Line K, Isupov MN, Littlechild JA (2004). Gamma lactamase from an Aureobacterium species in complex with 3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one.
Ohshiro T, Littlechild J, Garcia-Rodriguez E, Isupov MN, Iida Y, Kobayashi T, Izumi Y (2004). Modification of halogen specificity of a vanadium-dependent bromoperoxidase. Protein Sci, 13(6), 1566-1571. Abstract. Author URL.
Levdikov VM, Blagova EV, Brannigan JA, Cladière L, Antson AA, Isupov MN, Séror SJ, Wilkinson AJ (2004). The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability. Journal of Molecular Biology, 340(4), 767-782. Abstract.
Line K, Isupov MN, Littlechild JA (2004). The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site. J Mol Biol, 338(3), 519-532. Abstract. Author URL.
Guy JE, Isupov MN, Littlechild JA (2003). Crystallization and preliminary X-ray diffraction studies of a novel alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix. Acta Crystallogr D Biol Crystallogr, 59(Pt 1), 174-176. Abstract. Author URL.
Littlechild JA, Guy JE, Isupov MN (2003). The Structure of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix. Journal of Molecular Biology, 331(5), 1041-1051.
Hollingsworth EJ, Isupov MN, Littlechild JA (2002). Crystallization and preliminary X-ray diffraction analysis of L-aminoacylase from the hyperthermophilic archaeon Thermococcus litoralis. Acta Crystallogr D Biol Crystallogr, 58(Pt 3), 507-510. Abstract. Author URL.
Littlechild J, Garcia-Rodriguez E, Dalby A, Isupov M (2002). Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes. J Mol Recognit, 15(5), 291-296. Abstract. Author URL.
Qi JF, Isupov MN, Littlechild JA, Anderson LE (2001). Chloroplast glyceraldehyde-3-P dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 222, U121-U121. Author URL.
Qi JF, Isupov MN, Littlechild JA, Anderson LE (2001). Chloroplast glyceraldehyde-3-P dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. BIOCHEMISTRY, 40(29), 8615-8615. Author URL.
Littlechild JA, Anderson LE, Isupov MN, Qi JF (2001). Chloroplast glyceraldehydes-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit. Journal of Biological Chemistry, 276(38), 35247-35252.
Harris JR, Schröder E, Isupov MN, Scheffler D, Kristensen P, Littlechild JA, Vagin AA, Meissner U (2001). Comparison of the decameric structure of peroxiredoxin-II by transmission electron microscopy and X-ray crystallography. Biochim Biophys Acta, 1547(2), 221-234. Abstract. Author URL.
Gonsalvez IS, Isupov MN, Littlechild JA (2001). Crystallization and preliminary X-ray analysis of a gamma-lactamase. Acta Crystallogr D Biol Crystallogr, 57(Pt 2), 284-286. Abstract. Author URL.
Littlechild JA, Isupov M (2001). Glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus solfataricus. Methods Enzymol, 331, 105-117. Author URL.
Vagin AA, Isupov MN (2001). Spherically averaged phased translation function and its application to the search for molecules and fragments in electron-density maps. Acta Crystallographica - Section D Biological Crystallography, 57(10), 1451-1456. Abstract.
Littlechild J, Isupov M, Garcia E (2001). Structural studies on the dodecameric vanadium containing bromoperoxidase from <i>Corallina officinalis</i>. JOURNAL OF INORGANIC BIOCHEMISTRY, 86(1), 67-67. Author URL.
Winn MD, Isupov MN, Murshudov GN (2001). Use of TLS parameters to model anisotropic displacements in macromolecular refinement. Acta Crystallographica Section D: Biological Crystallography, 57(1), 122-133. Abstract.
Schröder E, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Isupov MN (2000). Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 a resolution. Structure, 8(6), 605-615. Abstract. Author URL.
Schröder E, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Isupov MN (2000). Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 Å resolution (vol 8, pg 605, 2000). STRUCTURE, 8(12), U5-U5. Author URL.
Isupov MN, Dalby AR, Brindley AA, Izumi Y, Tanabe T, Murshudov GN, Littlechild JA (2000). Crystal structure of dodecameric vanadium-dependent bromoperoxidase from the red algae Corallina officinalis. J Mol Biol, 299(4), 1035-1049. Abstract. Author URL.
Bourne PC, Isupov MN, Littlechild JA (2000). The atomic-resolution structure of a novel bacterial esterase. Structure, 8(2), 143-151. Abstract. Author URL.
Charron C, Talfournier F, Isupov MN, Littlechild JA, Branlant G, Vitoux B, Aubry A (2000). The crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus in the presence of NADP(+) at 2.1 a resolution. J Mol Biol, 297(2), 481-500. Abstract. Author URL.
Isupov MN, Littlechild JA, Lebedev AA, Errington N, Vagin AA, Schroder E (2000). thioredoxin peroxidase B from red blood cells.
Isupov MN, Fleming TM, Dalby AR, Crowhurst GS, Bourne PC, Littlechild JA (1999). Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. J Mol Biol, 291(3), 651-660. Abstract. Author URL.
Schröder E, Isupov MN, Naran A, Littlechild JA (1999). Crystallization and preliminary X-ray analysis of human thioredoxin peroxidase-B from red blood cells. Acta Crystallogr D Biol Crystallogr, 55(Pt 2), 536-538. Abstract. Author URL.
Charron C, Talfournier F, Isupov MN, Branlant G, Littlechild JA, Vitoux B, Aubry A (1999). Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus. Acta Crystallogr D Biol Crystallogr, 55(Pt 7), 1353-1355. Abstract. Author URL.
Singleton MR, Isupov MN, Littlechild JA (1999). Crystallization and preliminary X-ray diffraction studies of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Acta Crystallogr D Biol Crystallogr, 55(Pt 3), 702-703. Abstract. Author URL.
Bourne PC, Isupov MN, Littlechild JA (1999). Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase. Acta Crystallogr D Biol Crystallogr, 55(Pt 4), 915-917. Abstract. Author URL.
Crowhurst GS, Dalby AR, Isupov MN, Campbell JW, Littlechild JA (1999). Structure of a phosphoglycerate mutase:3-phosphoglyceric acid complex at 1.7 A. Acta Crystallogr D Biol Crystallogr, 55(Pt 11), 1822-1826. Abstract. Author URL.
Morgunova E, Tuuttila A, Bergmann U, Isupov M, Lindqvist Y, Schneider G, Tryggvason K (1999). Structure of human pro-matrix metalloproteinase-2: activation mechanism revealed. Science, 284(5420), 1667-1670. Abstract. Author URL.
Singleton M, Isupov M, Littlechild J (1999). X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Structure, 7(3), 237-244. Abstract. Author URL.
Fleming TM, Jones CE, Piper PW, Cowan DA, Isupov MN, Littlechild JA (1998). Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. Acta Crystallogr D Biol Crystallogr, 54(Pt 4), 671-674. Abstract. Author URL.
Isupov MN, Antson AA, Dodson EJ, Dodson GG, Dementieva IS, Zakomirdina LN, Wilson KS, Dauter Z, Lebedev AA, Harutyunyan EH, et al (1998). Crystal structure of tryptophanase. Journal of Molecular Biology, 276(3), 603-623. Abstract.
McGhie EJ, Isupov MN, Schröder E, Littlechild JA (1998). Crystallization and preliminary X-ray diffraction studies of the oxygenating subunit of 3,6-diketocamphane monooxygenase from Pseudomonas putida. Acta Crystallogr D Biol Crystallogr, 54(Pt 5), 1035-1038. Abstract. Author URL.
Brindley AA, Dalby AR, Isupov MN, Littlechild JA (1998). Preliminary X-ray analysis of a new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis. Acta Crystallogr D Biol Crystallogr, 54(Pt 3), 454-457. Abstract. Author URL.
Crowhurst GSE, Isupov MN, Fleming T, Littlechild JA (1998). Two glycolytic enzymes from <i>Sulfolobus solfataricus</i>. Biochemical Society Transactions, 26(3), S275-S275.
Pletnev SV, Antson AA, Sinitsyna NI, Dauter Z, Isupov MN, Hurs EN, Faleev NG, Wilson KS, Dodson G, Demidkina TV, et al (1997). Crystallographic study of tyrosine phenol-lyase from Erwinia herbicola. Kristallografiya, 42(5), 877-888.
Isupov MN, Obmolova G, Butterworth S, BadetDenisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A (1997). Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: Evidence from the 1.8 angstrom crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase (vol 4, pg 801, 1996). STRUCTURE, 5(5), 723-723. Author URL.
Isupov MN, Teplyakov A (1996). GLUTAMINASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH GLUTAMATE.
Isupov MN, Teplyakov A (1996). GLUTAMINASE DOMAIN OF GLUCOSAMINE 6-PHOSPHATE SYNTHASE COMPLEXED WITH L-GLU HYDROXAMATE.
Pletnev SV, Isupov MN, Dauter Z, Wilson KS, Faleev NG, Harutyunyan EG, Demidkina TV (1996). Purification and crystals of tyrosine phenol-lyase from Erwinia herbicola. Biochemistry and Molecular Biology International, 38(1 Included), 37-42. Abstract.
Isupov MN, Obmolova G, Butterworth S, Badet-Denisot MA, Badet B, Polikarpov I, Littlechild JA, Teplyakov A (1996). Substrate binding is required for assembly of the active conformation of the catalytic site in Ntn amidotransferases: evidence from the 1.8 a crystal structure of the glutaminase domain of glucosamine 6-phosphate synthase. Structure, 4(7), 801-810. Abstract. Author URL.
Dementieva IS, Zakomirdina LN, Sinitzina NI, Antson AA, Wilson KS, Isupov MN, Lebedev AA, Harutyunyan EH (1994). Crystallization and preliminary X-ray investigation of holotryptophanases from Escherichia coli and Proteus vulgaris. Journal of Molecular Biology, 235(2), 783-786. Abstract.
Strelkov SV, Zurabishvili TG, Nepluev IV, Efimov VP, Isupov MN, Harutyunyan EH, Mesyanzhinov VV (1993). Crystallization and preliminary crystallographic characterization of bacteriophage T4 baseplate protein encoded by gene 9. Journal of Molecular Biology, 234(2), 493-495. Abstract.
Antson AA, Strokopytov BV, Murshudov GN, Isupov MN, Harutyunyan EH, Demidkina TV, Vassylyev DG, Dauter Z, Terry H, Wilson KS, et al (1992). The polypeptide chain fold in tyrosine phenol-lyase, a pyridoxal-5′-phosphate-dependent enzyme. FEBS Letters, 302(3), 256-260. Abstract.
BOGDANKEVICH OV, DAVYDOV VO, ZVEREV MM, ISUPOV MN, KUDEYAROV YA, FAIFER VN (1987). INDUCED CONDUCTIVITY OF CADMIUM-SULFIDE CRYSTALS SUBJECTED TO STRONG EXCITATION. SOVIET PHYSICS SEMICONDUCTORS-USSR, 21(12), 1342-1343. Author URL.
Chapters
Littlechild J, Isupov M (2014). Haloperoxidase enzymes as 'redox catalysts' important for industrial biocatalysis. In (Ed) Recent Advances in Redox Active Plant and Microbial Products: from Basic Chemistry to Widespread Applications in Medicine and Agriculture, 425-446. Abstract.
Littlechild JA, Garcia-Rodriguez E, Coupe E, Watts A, Isupov M (2007). Structural Studies of Vanadium Haloperoxidases:Insight into Halide Specificity,Stability and Enzyme Mechanism. In Kustin K, Pessoa JC, Crans D (Eds.) Vanadium:The versatile Metal, Washington, USA: ACS, 136-147.
Isupov M, Dementieva I, Zakomirdina L, Wilson KS, Dauter Z, Antson AA, Dodson GG, Harutyunyan EG (1994). X-ray study of tryptophanase at 2.1 Å resolution. In Marino G, Sannia G, Bossa F (Eds.) Biochemistry of Vitamin B6 and PQQ, Basel: Birkhäuser Basel, 183-185. Abstract.
Conferences
Littlechild JA, Guy JE, Isupov MN (2004). Hyperthermophilic dehydrogenase enzymes. Abstract. Author URL.
Gonsalvez IS, Littlechild JA, Isupov M, Taylor SJ, Brown R (2000). Characterisation and preliminary crystallographic studies of a novel γ-lactamase enzyme used in the synthesis of an anti-HIV drug.
Hollingsworth EJ, Brown R, Isupov MN, Taylor S, Littlechild JA (2000). Cloning, Expression & Purification of a Thermostable Aminoacylase from <i>Thermococcus litoralis</i>.
Vagin A, Isupov M (2000). Spherically averaged phased translation function and its applications to search of molecules and fragments in the macromolecular electron density maps. Author URL.
Schröder E, Littlechild JA, Lebedev AA, Isupov MN (2000). Structure and mechanism of decameric 2-cys peroxiredoxin from human erythrocytes.
Schröder E, Lebedev AA, Isupov MN, Littlechild JA (2000). Structure and mechanism of human decameric 2-cys peroxiredoxin.
Winn MD, Isupov M, Murshudov GN (2000). Use of collective variables to model anisotropic displacements; implementation in the macromolecular refinement program REFMAC. Author URL.
Isupov MN, Dalby AR, Brindley AA, Littlechild JA (1999). CRYSTAL STRUCTURE OF THE VANADIUM DEPENDENT HALOPEROXIDASE FROM C. OFFICINALIS. Author URL.
Winn MD, Isupov M, Murshudov GN (1999). EFFECTS OF TLS THERMAL PARAMETERS IN MACROMOLECULAR REFINEMENT. Author URL.
Bourne PC, Isupov MN, Littlechild JA (1999). THE ATOMIC RESOLUTION (1.15Å) STRUCTURE OF a NOVEL BACTERIAL ESTERASE. Author URL.
Schroder E, Isupov MI, Littlechild JA (1999). X-RAY STRUCTURAL STUDIES WITH HUMAN THIOREDOXIN PEROXIDASE. Author URL.
Isupov MN, Lebedev AA, Harutyunyan EH, Antson FA, Wilson KS, Dodson GG, Zakomirdina LN, Littlechild JA, Dementieva IS (1999). X-RAY STUDIES OF TRYPTOPHANASE. Author URL.
Crowhurst G, Isupov MN, Littlechild JA (1999). X-RAY STUDIES OF TWO GLYCOLYTIC ENZYMES FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS. Author URL.
Crowhurst GS, Isupov MN, Fleming T, Littlechild JA (1998). Two glycolytic enzymes from Sulfolobus solfataricus. Author URL.
Lilly MD, Chauhan R, French C, Gyamerah M, Hobbs GR, Humphrey A, Isupov M, Littlechild JA, Mitra RK, Morris KG, et al (1996). Carbon-carbon bond synthesis: the impact of rDNA technology on the production and use of E. coli transketolase. Abstract.
Teplyakovi A, Isupov MN (1996). STRUCTURE DETERMINATION OF GLUCOSAMINE-6P SYNTHASE: FIGHTING AGAINST PSEUDOSYMMETRY. Author URL.
