-
- People
- Procedures and Guidelines
- Equipment
- Services
- Transmission Electron Microscopy (TEM) Techniques
- Light Microscopy (LM) Techniques
- Booking
- Gallery
Bioimaging Unit
Transmission Electron Microscopy (TEM) Techniques
The lab houses a JEOL JEM 1400 TEM with Compustage and high resolution digital image capture. This allows for fast image collection, random sampling and very accurate measuring. Experienced staff is here available to provide support in all areas of TEM.
Specialized techniques:
- Conventional resin-embedding
- Negative staining
- Immunogold labeling
- Freeze substitution
- Correlative Light Electron Microscopy (CLEM)
- Stereology
Typical applications for TEM:
- Tissue (plant and animal) and cell culture
- Suspensions cells (fungi, bacteria, blood cells, etc.)
- Monolayers (eg. for CLEM)
- Viruses
- Detergent-extracted cytoskeleton and organelles (eg. Golgi, Mithocondria, Vesicles)
- Particulate size and distribution in non-biological samples
