Correlative Light and Electron Microscopy (CLEM)

CLEM enables the acquisition of dynamic as well as ultrastructural information about the specimen of interest. This method allows for the selection of rare events or structural features in complex, heterogeneous samples. We are currently exploring various methods to combine light and electron microscopy. One way is to image specimens that adhere to a surface containing a finder grid. After life cell imaging the sample will be fixed and the finder grid can be used to locate the region of interest after processing the sample for electron microscopy and analysis/quantitation at the ultrastructural level can be achieved.