Conventional resin-embedding

Biological samples can be fixed chemically and embedded in the resin of choice. After initial aldehyde-fixation the specimen will be post-fixed and then dehydrated in an ethanol gradient before embedment in a resin. The polymerised samples can then be sectioned using an ultramicrotome (typically section-thickness < 100 nm) and the ultrastructure can be contrasted utilising heavy metal stains (uranyl acetate and/or lead citrate) before imaging.

Samples can be fixed upon arrival to the Bioimaging Centre to lead to embedded material typically within 4 days.

Ultrastructure of Zymoseptoria tritici