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Dr Fulvia Bono

Dr Fulvia Bono

Associate Professor

 7450

 +44 (0)1392 727450

 Living Systems Institute T02.12

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

My research group at the LSI aims at understanding the molecular mechanisms that underlie the cytoplasmic control of gene expression. In particular, we focus on mRNA localization and translational control with an interdisciplinary approach that combines biochemistry, structural biology as well as cell- and organism-based functional assays.

I am based in the Living Systems Institute

Qualifications

2000: PhD in molecular biology, University of Pavia, Italy

2004: Specialization in applied genetics (Master equivalent), University of Pavia, Italy

Career

2018-present: Associate Professor, Living Systems Institute, University of Exeter, UK

2011-present: Group Leader, Max Planck Institute for Developmental Biology, Tübingen, Germany

2008-2011: Project Leader, MPI for Developmental Biology, Tübingen, Germany

2002-2007: Postdoctoral fellow, EMBL - Heidelberg, Germany

Research

Research interests

Our group has a long-standing interest in basic mechanistic questions in RNA biology, such as how mRNAs are exported from the nucleus and regulated in the cytoplasm.

The localization of certain mRNAs at specific sites within the cytoplasm is a prevalent way to regulate gene expression. mRNA localization plays a crucial role in embryonic patterning, cell polarity and neuronal function in a wide range of organisms. Studies of RNA localization in model organisms have revealed many components of the process, some of which are conserved across eukaryotes. The correct cytoplasmic localization of mRNAs and their transport and silencing in messenger ribonucleoprotein particles (mRNPs) is dependent on the earliest steps of mRNA processing in
the nucleus. The core nuclear mRNP particles associate with further components in the cytoplasm, forming large and dynamic multimolecular assemblies, underlying the various steps or mRNA maturation, transport, silencing and eventual localized translation. Even though many components of these large mRNP particles are known, their detailed molecular organization and regulation are poorly understood. Our aim is to gather structural information of mRNP complexes involved in RNA localization. We use a multidisciplinary approach, combining biochemistry, biophysics, protein crystallography and fly genetics, to tackle the complex structure-function relationships regulating mRNA localization. Our work will elucidate the structural details and molecular mechanisms of how mRNP complexes regulate mRNA localization during development. Given that mRNA localization is a conserved process, our results are expected to elucidate the mechanistic and structural details of a conserved regulatory pathway with fundamental and often medically relevant roles in the cell.

Grants/Funding:

2015-2018                          DFG Research Grant in Research Unit FOR 2333

Macromolecular Complexes in mRNA translocation

2012-2017                          European Research Council (ERC) Starting Grant

Towards a mechanistic understanding of mRNA localization

2010-2013                          DFG Research Grant

Structural studies of a bidirectional transport receptor, Importin13

2009-2012                          Max Planck inter-Institutional Project Grant

Deep sequencing of the transcriptome of two marine annelid species for neurobiological, structural, and comparative genomic studies

Key publications | Publications by category | Publications by year

Key publications


Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F (2016). The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nature Structural and Molecular Biology, 23(8), 705-713. Abstract.
Grünwald M, Lazzaretti D, Bono F (2013). Structural basis for the nuclear export activity of Importin13. EMBO Journal, 32(6), 899-913. Abstract.
Kinkelin K, Veith K, Grünwald M, Bono F (2012). Crystal structure of a minimal eIF4E-Cup complex reveals a general mechanism of eIF4E regulation in translational repression. RNA, 18(9), 1624-1634. Abstract.
Bono F, Ebert J, Lorentzen E, Conti E (2006). The Crystal Structure of the Exon Junction Complex Reveals How it Maintains a Stable Grip on mRNA. Cell, 126(4), 713-725. Abstract.

Publications by category


Books

Cook A, Bono F, Jinek M, Conti E (2007). Structural biology of nucleocytoplasmic transport. Abstract.

Journal articles

Pries V, Nöcker C, Khan D, Johnen P, Hong Z, Tripathi A, Keller AL, Fitz M, Perruccio F, Filipuzzi I, et al (2018). Target Identification and Mechanism of Action of Picolinamide and Benzamide Chemotypes with Antifungal Properties. Cell Chemical Biology Abstract.
Schellhaus AK, Moreno-Andrés D, Chugh M, Yokoyama H, Moschopoulou A, De S, Bono F, Hipp K, Schäffer E, Antonin W, et al (2017). Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics. Scientific Reports, 7(1). Abstract.
Lazzaretti D, Bono F (2017). mRNA localization in metazoans: a structural perspective. RNA BIOLOGY, 14(11), 1473-1484. Author URL.
Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F (2016). The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nature Structural and Molecular Biology, 23(8), 705-713. Abstract.
Bono F (2014). Juggling key players in NMD initiation. Structure, 22(8), 1074-1075. Abstract.
Holder T, Basquin C, Ebert J, Randel N, Jollivet D, Conti E, Jékely G, Bono F (2013). Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability. Biol Direct, 8 Abstract.  Author URL.
Grünwald M, Lazzaretti D, Bono F (2013). Structural basis for the nuclear export activity of Importin13. EMBO Journal, 32(6), 899-913. Abstract.
Kinkelin K, Veith K, Grünwald M, Bono F (2012). Crystal structure of a minimal eIF4E-Cup complex reveals a general mechanism of eIF4E regulation in translational repression. RNA, 18(9), 1624-1634. Abstract.
Bono F, Gehring NH (2011). Assembly, disassembly and recycling the dynamics of exon junction complexes. RNA Biology, 8(1). Abstract.
Grünwald M, Bono F (2011). Structure of Importin13-Ubc9 complex: Nuclear import and release of a key regulator of sumoylation. EMBO Journal, 30(2), 427-438. Abstract.
Buchwald G, Ebert J, Basquin C, Sauliere J, Jayachandran U, Bono F, Le Hir H, Conti E (2010). Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex. Proceedings of the National Academy of Sciences of the United States of America, 107(22), 10050-10055. Abstract.
Bono F, Cook AG, Grünwald M, Ebert J, Conti E (2010). Nuclear Import Mechanism of the EJC Component Mago-Y14 Revealed by Structural Studies of Importin 13. Molecular Cell, 37(2), 211-222. Abstract.
Bono F, Ebert J, Lorentzen E, Conti E (2006). The Crystal Structure of the Exon Junction Complex Reveals How it Maintains a Stable Grip on mRNA. Cell, 126(4), 713-725. Abstract.
Bono F, Ebert J, Unterholzner L, Güttler T, Izaurralde E, Conti E (2004). Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex. EMBO Reports, 5(3), 304-310. Abstract.
Sabile A, Perlemuter G, Bono F, Kohara K, Demaugre F, Kohara M, Matsuura Y, Miyamura T, Bréchot C, Barba G, et al (1999). Hepatitis C virus core protein binds to apolipoprotein AII and its secretion is modulated by fibrates. Hepatology, 30(4), 1064-1076. Abstract.
Bruno S, Silini E, Crosignani A, Borzio F, Leandro G, Bono F, Asti M, Rossi S, Larghi A, Cerino A, et al (1997). Hepatitis C virus genotypes and risk of hepatocellular carcinoma in cirrhosis: a prospective study. Hepatology, 25(3), 754-758. Abstract.
Silini E, Bottelli R, Asti M, Bruno S, Candusso ME, Brambilla S, Bono F, Iamoni G, Tinelli C, Mondelli MU, et al (1996). Hepatitis C virus genotypes and risk of hepatocellular carcinoma in cirrhosis: a case-control study. Gastroenterology, 111(1), 199-205. Abstract.
Baldanti F, Silini E, Sarasini A, Talarico CL, Stanat SC, Biron KK, Furione M, Bono F, Palu G, Gerna G, et al (1995). A three-nucleotide deletion in the UL97 open reading frame is responsible for the ganciclovir resistance of a human cytomegalovirus clinical isolate. Journal of Virology, 69(2), 796-800. Abstract.
Silini E, Bono F, Cividini A, Cerino A, Bruno S, Rossi S, Belloni G, Brugnetti B, Civardi E, Salvaneschi L, et al (1995). Differential distribution of hepatitis C virus genotypes in patients with and without liver function abnormalities. Hepatology, 21(2), 285-290. Abstract.
Silini E, Bono F, Cividini A, Cerino A, Maccabruni A, Tinelli C, Bruno S, Bellobuono A, Mondelli MU (1995). Molecular epidemiology of hepatitis C virus infection among intravenous drug users. Journal of Hepatology, 22(6), 691-695. Abstract.
Mondelli MU, Cerino A, Bono F, Cividini A, Maccabruni A, Arico M, Malfitano A, Barbarini G, Piazza V, Minoli L, et al (1994). Hepatitis C virus (HCV) core serotypes in chronic HCV infection. Journal of Clinical Microbiology, 32(10), 2523-2527. Abstract.
Aricò M, Maggiore G, Silini E, Bono F, Viganò C, Cerino A, Mondelli MU (1994). Hepatitis C virus infection in children treated for acute lymphoblastic leukemia. Blood, 84(9), 2919-2922. Abstract.
Magrassi L, Butti G, Silini E, Bono F, Paoletti P, Milanesi G (1993). The expression of genes of the steroid-thyroid hormone receptor superfamily in central nervous system tumors. Anticancer Research, 13(4), 859-866. Abstract.
Silini E, Bono F, Cerino A, Piazza V, Solcia E, Mondelli MU (1993). Virological features of hepatitis C virus infection in hemodialysis patients. Journal of Clinical Microbiology, 31(11), 2913-2917. Abstract.
Magrassi L, Bono F, Milanesi G, Butti G (1992). Vitamin D receptor expression in human brain tumors. Journal of Neurosurgical Sciences, 36(1), 27-30.

Publications by year


2018

Pries V, Nöcker C, Khan D, Johnen P, Hong Z, Tripathi A, Keller AL, Fitz M, Perruccio F, Filipuzzi I, et al (2018). Target Identification and Mechanism of Action of Picolinamide and Benzamide Chemotypes with Antifungal Properties. Cell Chemical Biology Abstract.

2017

Schellhaus AK, Moreno-Andrés D, Chugh M, Yokoyama H, Moschopoulou A, De S, Bono F, Hipp K, Schäffer E, Antonin W, et al (2017). Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics. Scientific Reports, 7(1). Abstract.
Lazzaretti D, Bono F (2017). mRNA localization in metazoans: a structural perspective. RNA BIOLOGY, 14(11), 1473-1484. Author URL.

2016

Lazzaretti D, Veith K, Kramer K, Basquin C, Urlaub H, Irion U, Bono F (2016). The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nature Structural and Molecular Biology, 23(8), 705-713. Abstract.

2014

Bono F (2014). Juggling key players in NMD initiation. Structure, 22(8), 1074-1075. Abstract.

2013

Holder T, Basquin C, Ebert J, Randel N, Jollivet D, Conti E, Jékely G, Bono F (2013). Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability. Biol Direct, 8 Abstract.  Author URL.
Grünwald M, Lazzaretti D, Bono F (2013). Structural basis for the nuclear export activity of Importin13. EMBO Journal, 32(6), 899-913. Abstract.

2012

Kinkelin K, Veith K, Grünwald M, Bono F (2012). Crystal structure of a minimal eIF4E-Cup complex reveals a general mechanism of eIF4E regulation in translational repression. RNA, 18(9), 1624-1634. Abstract.

2011

Bono F, Gehring NH (2011). Assembly, disassembly and recycling the dynamics of exon junction complexes. RNA Biology, 8(1). Abstract.
Grünwald M, Bono F (2011). Structure of Importin13-Ubc9 complex: Nuclear import and release of a key regulator of sumoylation. EMBO Journal, 30(2), 427-438. Abstract.

2010

Buchwald G, Ebert J, Basquin C, Sauliere J, Jayachandran U, Bono F, Le Hir H, Conti E (2010). Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex. Proceedings of the National Academy of Sciences of the United States of America, 107(22), 10050-10055. Abstract.
Bono F, Cook AG, Grünwald M, Ebert J, Conti E (2010). Nuclear Import Mechanism of the EJC Component Mago-Y14 Revealed by Structural Studies of Importin 13. Molecular Cell, 37(2), 211-222. Abstract.

2007

Cook A, Bono F, Jinek M, Conti E (2007). Structural biology of nucleocytoplasmic transport. Abstract.

2006

Bono F, Ebert J, Lorentzen E, Conti E (2006). The Crystal Structure of the Exon Junction Complex Reveals How it Maintains a Stable Grip on mRNA. Cell, 126(4), 713-725. Abstract.

2004

Bono F, Ebert J, Unterholzner L, Güttler T, Izaurralde E, Conti E (2004). Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex. EMBO Reports, 5(3), 304-310. Abstract.

1999

Sabile A, Perlemuter G, Bono F, Kohara K, Demaugre F, Kohara M, Matsuura Y, Miyamura T, Bréchot C, Barba G, et al (1999). Hepatitis C virus core protein binds to apolipoprotein AII and its secretion is modulated by fibrates. Hepatology, 30(4), 1064-1076. Abstract.

1997

Bruno S, Silini E, Crosignani A, Borzio F, Leandro G, Bono F, Asti M, Rossi S, Larghi A, Cerino A, et al (1997). Hepatitis C virus genotypes and risk of hepatocellular carcinoma in cirrhosis: a prospective study. Hepatology, 25(3), 754-758. Abstract.

1996

Silini E, Bottelli R, Asti M, Bruno S, Candusso ME, Brambilla S, Bono F, Iamoni G, Tinelli C, Mondelli MU, et al (1996). Hepatitis C virus genotypes and risk of hepatocellular carcinoma in cirrhosis: a case-control study. Gastroenterology, 111(1), 199-205. Abstract.

1995

Baldanti F, Silini E, Sarasini A, Talarico CL, Stanat SC, Biron KK, Furione M, Bono F, Palu G, Gerna G, et al (1995). A three-nucleotide deletion in the UL97 open reading frame is responsible for the ganciclovir resistance of a human cytomegalovirus clinical isolate. Journal of Virology, 69(2), 796-800. Abstract.
Silini E, Bono F, Cividini A, Cerino A, Bruno S, Rossi S, Belloni G, Brugnetti B, Civardi E, Salvaneschi L, et al (1995). Differential distribution of hepatitis C virus genotypes in patients with and without liver function abnormalities. Hepatology, 21(2), 285-290. Abstract.
Silini E, Bono F, Cividini A, Cerino A, Maccabruni A, Tinelli C, Bruno S, Bellobuono A, Mondelli MU (1995). Molecular epidemiology of hepatitis C virus infection among intravenous drug users. Journal of Hepatology, 22(6), 691-695. Abstract.

1994

Mondelli MU, Cerino A, Bono F, Cividini A, Maccabruni A, Arico M, Malfitano A, Barbarini G, Piazza V, Minoli L, et al (1994). Hepatitis C virus (HCV) core serotypes in chronic HCV infection. Journal of Clinical Microbiology, 32(10), 2523-2527. Abstract.
Aricò M, Maggiore G, Silini E, Bono F, Viganò C, Cerino A, Mondelli MU (1994). Hepatitis C virus infection in children treated for acute lymphoblastic leukemia. Blood, 84(9), 2919-2922. Abstract.

1993

Magrassi L, Butti G, Silini E, Bono F, Paoletti P, Milanesi G (1993). The expression of genes of the steroid-thyroid hormone receptor superfamily in central nervous system tumors. Anticancer Research, 13(4), 859-866. Abstract.
Silini E, Bono F, Cerino A, Piazza V, Solcia E, Mondelli MU (1993). Virological features of hepatitis C virus infection in hemodialysis patients. Journal of Clinical Microbiology, 31(11), 2913-2917. Abstract.

1992

Magrassi L, Bono F, Milanesi G, Butti G (1992). Vitamin D receptor expression in human brain tumors. Journal of Neurosurgical Sciences, 36(1), 27-30.

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