Overview
Please see my lab website.
Dr Charlie Jeynes is an STFC 'Innovation' Research Fellow working at the Centre for Biomedical Modelling and Analysis, University of Exeter, U.K. This centre is an Institutional Strategic Support Fund, with the aim of developing computational analysis and mathematical modelling within the life sciences.
After studying for a degree in Biochemistry and Genetics at the University of Wales, Cardiff, he moved to the University of Surrey where he was awarded a PhD functionalising carbon nanotubes with DNA.
He then worked as a Post-Doc for the CONFORM consortium, a collaboration between a number of institutions including the Universities of Oxford/Surrey/Manchester and the Cockcroft Institute, investigating next generation particle accelerators largely for cancer treatment.
Following this, he worked as the scientific officer for the U.K. National Ion Beam Centre, facilitating beam access for various institutions, as well as pursuing his own research interests into nanoparticle enhanced radiation therapies.
He then moved to the University of Reading where he completed his Higher Education associate fellow training (AFHFA) as a Teaching Fellow.
While at the University of Exeter, he has worked as an independant Wellcome Trust Fellow at the Centre for Biomedical Modelling and Analysis, working with a range of academics including Prof Christian Soeller and Prof John Terry - largely on correlative single molecule imaging technologies using nanoparticles.
He was recently awarded an STFC 'Innovation' Research Fellowship working with http://emps.exeter.ac.uk/physics-astronomy/staff/tjharrie Tim Harries to pursue modelling and experimental techniques to investigate how nanoparticles can be used in Photodynamic Therapy.
Broad research specialisms:
- X-ray fluorescence microscopy
- Single Molecule Imaging
- Image Processing
- Radiation Biology
- Nanotoxicology
- Nanotechnology
Qualifications
2015 Associate Fellow Higher Education Academy
2003-2007 Ph.D, Bio-nanotechnology, University of Surrey
1999-2003 B.Sc, Biochemistry and Genetics, University of Cardiff (year in industry Merck KgA)
Career
|
07/2015-present
|
University of Exeter – STFC ‘Innovation’ Research Fellow –
A 3 year Fellowship, in collaboration with Prof Tim Harries (UoE), to continue my research in metal nanoparticle probes for cancer photodynamic therapy
|
|
06/2015-07/2018
|
University of Exeter – Wellcome Trust ISSF Research Fellow –
A highly competitive award with £10K consumables to pursue my own research interests; supported by the Institution Science Support Funded (ISSF) Centre of Biomedical Modelling and Analysis; 50% of my time was spent using my skills in quantitative imaging with academics in Exeter ad beyond.
|
|
07/2014-06/2015
|
University of Reading – Teaching Fellow
I lectured large (>300) and small (<5) groups mainly biochemistry related modules. I also obtained my Higher Education Award.
|
|
06/2012-06/2014
|
UK National Ion Beam Centre (Surrey) – Scientific Experimental Officer
My role was to facilitate experiments from a range of scientists on the proton induced X-ray fluorescent imaging spectroscopy beamline
|
|
07/2007-06/2012
|
CONFORM project (Universities of Oxford, Surrey & Cockcroft Institute)
Post-doc position where I developed a proton beamline capable of irradiating and analysing human tissue – for applications in proton cancer therapy
|
|
06/2001-06/2002
|
Merck KgA, Newport, UK (industrial placement)
|
Research
Research interests
The broad theme of my work is to investigate nanoparticles for human health. This includes studying the toxic effect of nanoparticles from air pollution; using nanoparticles to develop new tools to probe biological processes on a single molecule level; as well as developing novel drugs based on nanoparticle designs.
Key to my research is using analytical imaging methods to quantify nanoparticles in human cells and tissue.
I use a range of imaging techniques including electron microscopy, chemical imaging (Infra-Red/Raman), X-ray fluorescence microscopy (see IBC, Diamond Synchrotron) optical super resolution microscopy and fluorescent imaging.
I am also involved in a range of “seed corn” projects with academics at the University of Exeter supported through the Centre for Biomedical Modelling and Analysis. These projects involve utilising my analytical imaging skills for novel applications.
Research projects
Please see my lab website for current/past research projects.
Grants/funding
|
06/2018-08/2018
|
BBSRC-Research Experience Placement student (2.5K, I was awarded this to host a student at UoE to assist me in advancing qCLEM probes, and for their own career development, with myself as Principle Supervisor)
|
|
03/2017-present
|
Science and Technologies Facilities Council (£6K, A workshop to bring together simulation experts, image specialist and experimentalists to develop multi-scale models in biology, Queen’s Belfast April 2018)
|
|
02/2017-present
|
EPSRC (£9.8K, beamtime funding for UK National Ion Beam Centre [IBC])
The IBC is an EPSRC medium scale facility offering competitive beamtime access (PI with F.Palombo, Exeter)
|
|
09/2016-09/2017
|
Royal Society (£3K, Partnership Award - see outreach section)
|
|
03/2016-092016
|
Royal Society (£3K, International Exchange Award)
This was a personal award to visit my collaborator Prof Andrew Bettiol @ National University of Singapore to work on X-ray nano-imaging
|
|
02/201607-2016
|
Diamond Lightsource Synchrotron (‘in kind’ £10K Beamtime Access Award)
Highly competitive beamtime access granted through peer review & panel
|
|
09/2015-09/2016
|
Royal Society of Chemistry (£1K, Partnership Award – see outreach section)
|
|
06/2015-present
|
Wellcome Trust ‘Seed Corn’ ISSF Award (£10K)
|
Publications
Key publications | Publications by category | Publications by year
Publications by category
Journal articles
Jeynes JCG, Geraki K, Jeynes C, Zhaohong M, Bettiol AA, Latorre E, Harries LW, Soeller C (In Press). Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe.
ACS Nano,
11(12), 12632-12640.
Abstract:
Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe.
Techniques to analyze human telomeres are imperative in studying the molecular mechanism of aging and related diseases. Two important aspects of telomeres are their length in DNA base pairs (bps) and their biophysical nanometer dimensions. However, there are currently no techniques that can simultaneously measure these quantities in individual cell nuclei. Here, we develop and evaluate a telomere "dual" gold nanoparticle-fluorescent probe simultaneously compatible with both X-ray fluorescence (XRF) and super resolution microscopy. We used silver enhancement to independently visualize the spatial locations of gold nanoparticles inside the nuclei, comparing to a standard QFISH (quantitative fluorescence in situ hybridization) probe, and showed good specificity at ∼90%. For sensitivity, we calculated telomere length based on a DNA/gold binding ratio using XRF and compared to quantitative polymerase chain reaction (qPCR) measurements. The sensitivity was low (∼10%), probably because of steric interference prohibiting the relatively large 10 nm gold nanoparticles access to DNA space. We then measured the biophysical characteristics of individual telomeres using super resolution microscopy. Telomeres that have an average length of ∼10 kbps, have diameters ranging between ∼60-300 nm. Further, we treated cells with a telomere-shortening drug and showed there was a small but significant difference in telomere diameter in drug-treated vs control cells. We discuss our results in relation to the current debate surrounding telomere compaction.
Abstract.
Author URL.
Full text.
Latorre E, Birar VC, Sheerin AN, Jeynes JCC, Hooper A, Dawe HR, Melzer D, Cox LS, Faragher RGA, Ostler EL, et al (In Press). Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence.
BMC Cell Biol,
18(1).
Abstract:
Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence.
BACKGROUND: Altered expression of mRNA splicing factors occurs with ageing in vivo and is thought to be an ageing mechanism. The accumulation of senescent cells also occurs in vivo with advancing age and causes much degenerative age-related pathology. However, the relationship between these two processes is opaque. Accordingly we developed a novel panel of small molecules based on resveratrol, previously suggested to alter mRNA splicing, to determine whether altered splicing factor expression had potential to influence features of replicative senescence. RESULTS: Treatment with resveralogues was associated with altered splicing factor expression and rescue of multiple features of senescence. This rescue was independent of cell cycle traverse and also independent of SIRT1, SASP modulation or senolysis. Under growth permissive conditions, cells demonstrating restored splicing factor expression also demonstrated increased telomere length, re-entered cell cycle and resumed proliferation. These phenomena were also influenced by ERK antagonists and agonists. CONCLUSIONS: This is the first demonstration that moderation of splicing factor levels is associated with reversal of cellular senescence in human primary fibroblasts. Small molecule modulators of such targets may therefore represent promising novel anti-degenerative therapies.
Abstract.
Author URL.
Full text.
Palombo F, Tamagnini F, Jeynes J, Mattana S, Swift I, Nallala J, Hancock J, Brown J, Randall A, Stone N, et al (2018). Detection of Aβ plaque-associated astrogliosis in Alzheimer’s disease brain by spectroscopic imaging and immunohistochemistry.
Analyst,
143, 850-857.
Full text.
Georgantzoglou A, Merchant MJ, Jeynes JCG, Mayhead N, Punia N, Butler RE, Jena R (2016). Applications of high-throughput clonogenic survival assays in high-LET particle microbeams.
Frontiers in Oncology,
5(JAN).
Abstract:
Applications of high-throughput clonogenic survival assays in high-LET particle microbeams
© 2016 Georgantzoglou, Merchant, Jeynes, Mayhead, Punia, Butler and Jena. Charged particle therapy is increasingly becoming a valuable tool in cancer treatment, mainly due to the favorable interaction of particle radiation with matter. Its application is still limited due, in part, to lack of data regarding the radiosensitivity of certain cell lines to this radiation type, especially to high-linear energy transfer (LET) particles. From the earliest days of radiation biology, the clonogenic survival assay has been used to provide radiation response data. This method produces reliable data but it is not optimized for high-throughput microbeam studies with high-LET radiation where high levels of cell killing lead to a very low probability of maintaining cells' clonogenic potential. A new method, therefore, is proposed in this paper, which could potentially allow these experiments to be conducted in a high-throughput fashion. Cells are seeded in special polypropylene dishes and bright-field illumination provides cell visualization. Digital images are obtained and cell detection is applied based on corner detection, generating individual cell targets as x-y points. These points in the dish are then irradiated individually by a micron field size high-LET microbeam. Post-irradiation, time-lapse imaging follows cells' response. All irradiated cells are tracked by linking trajectories in all time-frames, based on finding their nearest position. Cell divisions are detected based on cell appearance and individual cell temporary corner density. The number of divisions anticipated is low due to the high probability of cell killing from high-LET irradiation. Survival curves are produced based on cell's capacity to divide at least four to five times. The process is repeated for a range of doses of radiation. Validation shows the efficiency of the proposed cell detection and tracking method in finding cell divisions.
Abstract.
Jeynes JCG, Jeynes C, Palitsin V, Townley HE (2016). Direct quantification of rare earth doped titania nanoparticles in individual human cells.
NANOTECHNOLOGY,
27(28).
Author URL.
Georgantzoglou A, Merchant MJ, Jeynes JCG, Wera A-C, Kirkby KJ, Kirkby NF, Jena R (2015). Automatic cell detection in bright-field microscopy for microbeam irradiation studies.
PHYSICS IN MEDICINE AND BIOLOGY,
60(16), 6289-6303.
Author URL.
Barazzuol L, Jeynes JCG, Merchant MJ, Wera A-C, Barry MA, Kirkby KJ, Suzuki M (2015). Radiosensitization of glioblastoma cells using a histone deacetylase inhibitor (SAHA) comparing carbon ions with X-rays.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,
91(1), 90-98.
Author URL.
Mohamad AS, Jeynes JCG, Hughes MP (2014). Dielectrophoretic Response of DNA Shows Different Conduction Mechanisms for Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) in Solution.
IEEE TRANSACTIONS ON NANOBIOSCIENCE,
13(1), 51-54.
Author URL.
Wera A-C, Barazzuol L, Jeynes JCG, Merchant MJ, Suzuki M, Kirkby KJ (2014). Influence of the nucleus area distribution on the survival fraction after charged particles broad beam irradiation.
PHYSICS IN MEDICINE AND BIOLOGY,
59(15), 4197-4211.
Author URL.
Jeynes JCG, Merchant MJ, Spindler A, Wera A-C, Kirkby KJ (2014). Investigation of gold nanoparticle radiosensitization mechanisms using a free radical scavenger and protons of different energies.
PHYSICS IN MEDICINE AND BIOLOGY,
59(21), 6431-6443.
Author URL.
Jeynes JCG, Merchant MJ, Barazzuol L, Barry M, Guest D, Palitsin VV, Grime GW, Tullis IDC, Barber PR, Vojnovic B, et al (2013). "Broadbeam" irradiation of mammalian cells using a vertical microbeam facility.
RADIATION AND ENVIRONMENTAL BIOPHYSICS,
52(4), 513-521.
Author URL.
Barazzuol L, Jena R, Burnet NG, Meira LB, Jeynes JCG, Kirkby KJ, Kirkby NF (2013). Evaluation of poly (ADP-ribose) polymerase inhibitor ABT-888 combined with radiotherapy and temozolomide in glioblastoma.
RADIATION ONCOLOGY,
8 Author URL.
Jeynes JCG, Jeynes C, Merchant MJ, Kirkby KJ (2013). Measuring and modelling cell-to-cell variation in uptake of gold nanoparticles.
ANALYST,
138(23), 7070-7074.
Author URL.
Merchant MJ, Jeynes JCG, Grime GW, Palitsin V, Tullis IDW, Barber PR, Vojnovic B, Webb RP, Kirkby KJ (2012). A Focused Scanning Vertical Beam for Charged Particle Irradiation of Living Cells with Single Counted Particles.
RADIATION RESEARCH,
178(3), 182-190.
Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological effectiveness on live cells of laser driven protons at dose rates exceeding 10(9) Gy/s.
AIP ADVANCES,
2(1).
Author URL.
Barazzuol L, Jena R, Burnet NG, Jeynes JCG, Merchant MJ, Kirkby KJ, Kirkby NF (2012). In Vitro Evaluation of Combined Temozolomide and Radiotherapy Using X Rays and High-Linear Energy Transfer Radiation for Glioblastoma.
RADIATION RESEARCH,
177(5), 651-662.
Author URL.
Fiorini F, Kirby D, Borghesi M, Doria D, Jeynes JCG, Kakolee KF, Kar S, Kaur S, Kirby KJ, Merchant MJ, et al (2011). Dosimetry and spectral analysis of a radiobiological experiment using laser-driven proton beams.
PHYSICS IN MEDICINE AND BIOLOGY,
56(21), 6969-6982.
Author URL.
Jeynes JCG, Webb M, Kirkby KJ, Kirkby NF, Bradley DA (2009). Proceedings of the First International Conference on Biomedical Applications of High Energy Ion Beams Preface.
APPLIED RADIATION AND ISOTOPES,
67(3), 369-370.
Author URL.
Jeynes JCG, Mendoza E, Chow DCS, Watts PCR, McFadden J, Silva SRP (2006). Generation of chemically unmodified pure single-walled carbon nanotubes by solubilizing with RNA and treatment with ribonuclease A.
ADVANCED MATERIALS,
18(12), 1598-+.
Author URL.
Newcombe J, Jeynes JC, Mendoza E, Hinds J, Marsden GL, Stabler RA, Marti M, McFadden JJ (2005). Phenotypic and transcriptional characterization of the meningococcal PhoPQ system, a magnesium-sensing two-component regulatory system that controls genes involved in remodeling the meningococcal cell surface.
J Bacteriol,
187(14), 4967-4975.
Abstract:
Phenotypic and transcriptional characterization of the meningococcal PhoPQ system, a magnesium-sensing two-component regulatory system that controls genes involved in remodeling the meningococcal cell surface.
We previously identified and characterized a two-component regulatory system in the meningococcus with homology to the phoP-phoQ system in salmonella and showed that allele replacement of the NMB0595 regulator gene led to loss of virulence, sensitivity to antimicrobial peptides, perturbed protein expression, and magnesium-sensitive growth. On the basis of these findings we proposed that the system should be designated the meningococcal PhoPQ system. Here we further characterized the NMB0595 mutant and demonstrated that it had increased membrane permeability and was unable to form colonies on solid media with low magnesium concentrations, features that are consistent with disruption of PhoPQ-mediated modifications to the lipooligosaccharide structure. We examined the transcriptional profiles of wild-type and NMB0595 mutant strains and found that magnesium-regulated changes in gene expression are completely abrogated in the mutant, indicating that, similar to the salmonella PhoPQ system, the meningococcal PhoPQ system is regulated by magnesium. Transcriptional profiling of the mutant indicated that, also similar to the salmonella PhoPQ system, the meningococcal system is involved in control of virulence and remodeling of the bacterial cell surface in response to the host environment. The results are consistent with the hypothesis that the PhoP homologue plays a role in the meningococcus similar to the role played by PhoP in salmonella. Elucidating the role that the PhoPQ system and PhoPQ-regulated genes play in the response of the meningococcus to the host environment may provide new insights into the pathogenic process.
Abstract.
Author URL.
Conferences
Kar S, Doria D, Kakolee KF, Prasad R, Litt S, Ahmed H, Nersisyan G, Lewis C, Zepf M, Borghesi M, et al (2013). First Results on Cell Irradiation with laser-driven protons on the TARANIS system.
Author URL.
Prakrajang K, Jeynes JCG, Merchant MJ, Kirkby K, Kirkby N, Thopan P, Yu LD (2013). MeV single-ion beam irradiation of mammalian cells using the Surrey vertical nanobeam, compared with broad proton beam and X-ray irradiations.
Author URL.
Hanton F, Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, et al (2013). Radiobiology at ultra-high dose rates employing laser-driven ions.
Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological Cell Irradiation at Ultrahigh Dose Rate Employing Laser Driven Protons.
Author URL.
Jeynes JCG, Bailey MJ, Coley H, Kirkby KJ, Jeynes C (2010). Microbeam PIXE analysis of platinum resistant and sensitive ovarian cancer cells.
Author URL.
Jeynes JCG, Jeynes C, Kirkby KJ, Ruemmeli A, Silva SRP (2008). RBS/EBS/PIXE measurement of single-walled carbon nanotube modification by nitric acid purification treatment.
Author URL.
Jeynes JCG, Mendoza E, Chow D, McFadden J, Silva SRP (2005). DNA/RNA purified single walled carbon nanotubes on self-assembled networks.
Abstract:
DNA/RNA purified single walled carbon nanotubes on self-assembled networks
Abstract.
Publications by year
In Press
Jeynes JCG, Geraki K, Jeynes C, Zhaohong M, Bettiol AA, Latorre E, Harries LW, Soeller C (In Press). Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe.
ACS Nano,
11(12), 12632-12640.
Abstract:
Nanoscale Properties of Human Telomeres Measured with a Dual Purpose X-ray Fluorescence and Super Resolution Microscopy Gold Nanoparticle Probe.
Techniques to analyze human telomeres are imperative in studying the molecular mechanism of aging and related diseases. Two important aspects of telomeres are their length in DNA base pairs (bps) and their biophysical nanometer dimensions. However, there are currently no techniques that can simultaneously measure these quantities in individual cell nuclei. Here, we develop and evaluate a telomere "dual" gold nanoparticle-fluorescent probe simultaneously compatible with both X-ray fluorescence (XRF) and super resolution microscopy. We used silver enhancement to independently visualize the spatial locations of gold nanoparticles inside the nuclei, comparing to a standard QFISH (quantitative fluorescence in situ hybridization) probe, and showed good specificity at ∼90%. For sensitivity, we calculated telomere length based on a DNA/gold binding ratio using XRF and compared to quantitative polymerase chain reaction (qPCR) measurements. The sensitivity was low (∼10%), probably because of steric interference prohibiting the relatively large 10 nm gold nanoparticles access to DNA space. We then measured the biophysical characteristics of individual telomeres using super resolution microscopy. Telomeres that have an average length of ∼10 kbps, have diameters ranging between ∼60-300 nm. Further, we treated cells with a telomere-shortening drug and showed there was a small but significant difference in telomere diameter in drug-treated vs control cells. We discuss our results in relation to the current debate surrounding telomere compaction.
Abstract.
Author URL.
Full text.
Latorre E, Birar VC, Sheerin AN, Jeynes JCC, Hooper A, Dawe HR, Melzer D, Cox LS, Faragher RGA, Ostler EL, et al (In Press). Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence.
BMC Cell Biol,
18(1).
Abstract:
Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence.
BACKGROUND: Altered expression of mRNA splicing factors occurs with ageing in vivo and is thought to be an ageing mechanism. The accumulation of senescent cells also occurs in vivo with advancing age and causes much degenerative age-related pathology. However, the relationship between these two processes is opaque. Accordingly we developed a novel panel of small molecules based on resveratrol, previously suggested to alter mRNA splicing, to determine whether altered splicing factor expression had potential to influence features of replicative senescence. RESULTS: Treatment with resveralogues was associated with altered splicing factor expression and rescue of multiple features of senescence. This rescue was independent of cell cycle traverse and also independent of SIRT1, SASP modulation or senolysis. Under growth permissive conditions, cells demonstrating restored splicing factor expression also demonstrated increased telomere length, re-entered cell cycle and resumed proliferation. These phenomena were also influenced by ERK antagonists and agonists. CONCLUSIONS: This is the first demonstration that moderation of splicing factor levels is associated with reversal of cellular senescence in human primary fibroblasts. Small molecule modulators of such targets may therefore represent promising novel anti-degenerative therapies.
Abstract.
Author URL.
Full text.
2018
Palombo F, Tamagnini F, Jeynes J, Mattana S, Swift I, Nallala J, Hancock J, Brown J, Randall A, Stone N, et al (2018). Detection of Aβ plaque-associated astrogliosis in Alzheimer’s disease brain by spectroscopic imaging and immunohistochemistry.
Analyst,
143, 850-857.
Full text.
2016
Georgantzoglou A, Merchant MJ, Jeynes JCG, Mayhead N, Punia N, Butler RE, Jena R (2016). Applications of high-throughput clonogenic survival assays in high-LET particle microbeams.
Frontiers in Oncology,
5(JAN).
Abstract:
Applications of high-throughput clonogenic survival assays in high-LET particle microbeams
© 2016 Georgantzoglou, Merchant, Jeynes, Mayhead, Punia, Butler and Jena. Charged particle therapy is increasingly becoming a valuable tool in cancer treatment, mainly due to the favorable interaction of particle radiation with matter. Its application is still limited due, in part, to lack of data regarding the radiosensitivity of certain cell lines to this radiation type, especially to high-linear energy transfer (LET) particles. From the earliest days of radiation biology, the clonogenic survival assay has been used to provide radiation response data. This method produces reliable data but it is not optimized for high-throughput microbeam studies with high-LET radiation where high levels of cell killing lead to a very low probability of maintaining cells' clonogenic potential. A new method, therefore, is proposed in this paper, which could potentially allow these experiments to be conducted in a high-throughput fashion. Cells are seeded in special polypropylene dishes and bright-field illumination provides cell visualization. Digital images are obtained and cell detection is applied based on corner detection, generating individual cell targets as x-y points. These points in the dish are then irradiated individually by a micron field size high-LET microbeam. Post-irradiation, time-lapse imaging follows cells' response. All irradiated cells are tracked by linking trajectories in all time-frames, based on finding their nearest position. Cell divisions are detected based on cell appearance and individual cell temporary corner density. The number of divisions anticipated is low due to the high probability of cell killing from high-LET irradiation. Survival curves are produced based on cell's capacity to divide at least four to five times. The process is repeated for a range of doses of radiation. Validation shows the efficiency of the proposed cell detection and tracking method in finding cell divisions.
Abstract.
Jeynes JCG, Jeynes C, Palitsin V, Townley HE (2016). Direct quantification of rare earth doped titania nanoparticles in individual human cells.
NANOTECHNOLOGY,
27(28).
Author URL.
2015
Georgantzoglou A, Merchant MJ, Jeynes JCG, Wera A-C, Kirkby KJ, Kirkby NF, Jena R (2015). Automatic cell detection in bright-field microscopy for microbeam irradiation studies.
PHYSICS IN MEDICINE AND BIOLOGY,
60(16), 6289-6303.
Author URL.
Barazzuol L, Jeynes JCG, Merchant MJ, Wera A-C, Barry MA, Kirkby KJ, Suzuki M (2015). Radiosensitization of glioblastoma cells using a histone deacetylase inhibitor (SAHA) comparing carbon ions with X-rays.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,
91(1), 90-98.
Author URL.
2014
Mohamad AS, Jeynes JCG, Hughes MP (2014). Dielectrophoretic Response of DNA Shows Different Conduction Mechanisms for Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) in Solution.
IEEE TRANSACTIONS ON NANOBIOSCIENCE,
13(1), 51-54.
Author URL.
Wera A-C, Barazzuol L, Jeynes JCG, Merchant MJ, Suzuki M, Kirkby KJ (2014). Influence of the nucleus area distribution on the survival fraction after charged particles broad beam irradiation.
PHYSICS IN MEDICINE AND BIOLOGY,
59(15), 4197-4211.
Author URL.
Jeynes JCG, Merchant MJ, Spindler A, Wera A-C, Kirkby KJ (2014). Investigation of gold nanoparticle radiosensitization mechanisms using a free radical scavenger and protons of different energies.
PHYSICS IN MEDICINE AND BIOLOGY,
59(21), 6431-6443.
Author URL.
2013
Jeynes JCG, Merchant MJ, Barazzuol L, Barry M, Guest D, Palitsin VV, Grime GW, Tullis IDC, Barber PR, Vojnovic B, et al (2013). "Broadbeam" irradiation of mammalian cells using a vertical microbeam facility.
RADIATION AND ENVIRONMENTAL BIOPHYSICS,
52(4), 513-521.
Author URL.
Barazzuol L, Jena R, Burnet NG, Meira LB, Jeynes JCG, Kirkby KJ, Kirkby NF (2013). Evaluation of poly (ADP-ribose) polymerase inhibitor ABT-888 combined with radiotherapy and temozolomide in glioblastoma.
RADIATION ONCOLOGY,
8 Author URL.
Kar S, Doria D, Kakolee KF, Prasad R, Litt S, Ahmed H, Nersisyan G, Lewis C, Zepf M, Borghesi M, et al (2013). First Results on Cell Irradiation with laser-driven protons on the TARANIS system.
Author URL.
Prakrajang K, Jeynes JCG, Merchant MJ, Kirkby K, Kirkby N, Thopan P, Yu LD (2013). MeV single-ion beam irradiation of mammalian cells using the Surrey vertical nanobeam, compared with broad proton beam and X-ray irradiations.
Author URL.
Jeynes JCG, Jeynes C, Merchant MJ, Kirkby KJ (2013). Measuring and modelling cell-to-cell variation in uptake of gold nanoparticles.
ANALYST,
138(23), 7070-7074.
Author URL.
Hanton F, Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, et al (2013). Radiobiology at ultra-high dose rates employing laser-driven ions.
Author URL.
2012
Merchant MJ, Jeynes JCG, Grime GW, Palitsin V, Tullis IDW, Barber PR, Vojnovic B, Webb RP, Kirkby KJ (2012). A Focused Scanning Vertical Beam for Charged Particle Irradiation of Living Cells with Single Counted Particles.
RADIATION RESEARCH,
178(3), 182-190.
Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological Cell Irradiation at Ultrahigh Dose Rate Employing Laser Driven Protons.
Author URL.
Doria D, Kakolee KF, Kar S, Litt SK, Fiorini F, Ahmed H, Green S, Jeynes JCG, Kavanagh J, Kirby D, et al (2012). Biological effectiveness on live cells of laser driven protons at dose rates exceeding 10(9) Gy/s.
AIP ADVANCES,
2(1).
Author URL.
Barazzuol L, Jena R, Burnet NG, Jeynes JCG, Merchant MJ, Kirkby KJ, Kirkby NF (2012). In Vitro Evaluation of Combined Temozolomide and Radiotherapy Using X Rays and High-Linear Energy Transfer Radiation for Glioblastoma.
RADIATION RESEARCH,
177(5), 651-662.
Author URL.
2011
Fiorini F, Kirby D, Borghesi M, Doria D, Jeynes JCG, Kakolee KF, Kar S, Kaur S, Kirby KJ, Merchant MJ, et al (2011). Dosimetry and spectral analysis of a radiobiological experiment using laser-driven proton beams.
PHYSICS IN MEDICINE AND BIOLOGY,
56(21), 6969-6982.
Author URL.
2010
Jeynes JCG, Bailey MJ, Coley H, Kirkby KJ, Jeynes C (2010). Microbeam PIXE analysis of platinum resistant and sensitive ovarian cancer cells.
Author URL.
2009
Jeynes JCG, Webb M, Kirkby KJ, Kirkby NF, Bradley DA (2009). Proceedings of the First International Conference on Biomedical Applications of High Energy Ion Beams Preface.
APPLIED RADIATION AND ISOTOPES,
67(3), 369-370.
Author URL.
2008
Jeynes JCG, Jeynes C, Kirkby KJ, Ruemmeli A, Silva SRP (2008). RBS/EBS/PIXE measurement of single-walled carbon nanotube modification by nitric acid purification treatment.
Author URL.
2006
Jeynes JCG, Mendoza E, Chow DCS, Watts PCR, McFadden J, Silva SRP (2006). Generation of chemically unmodified pure single-walled carbon nanotubes by solubilizing with RNA and treatment with ribonuclease A.
ADVANCED MATERIALS,
18(12), 1598-+.
Author URL.
2005
Jeynes JCG, Mendoza E, Chow D, McFadden J, Silva SRP (2005). DNA/RNA purified single walled carbon nanotubes on self-assembled networks.
Abstract:
DNA/RNA purified single walled carbon nanotubes on self-assembled networks
Abstract.
Newcombe J, Jeynes JC, Mendoza E, Hinds J, Marsden GL, Stabler RA, Marti M, McFadden JJ (2005). Phenotypic and transcriptional characterization of the meningococcal PhoPQ system, a magnesium-sensing two-component regulatory system that controls genes involved in remodeling the meningococcal cell surface.
J Bacteriol,
187(14), 4967-4975.
Abstract:
Phenotypic and transcriptional characterization of the meningococcal PhoPQ system, a magnesium-sensing two-component regulatory system that controls genes involved in remodeling the meningococcal cell surface.
We previously identified and characterized a two-component regulatory system in the meningococcus with homology to the phoP-phoQ system in salmonella and showed that allele replacement of the NMB0595 regulator gene led to loss of virulence, sensitivity to antimicrobial peptides, perturbed protein expression, and magnesium-sensitive growth. On the basis of these findings we proposed that the system should be designated the meningococcal PhoPQ system. Here we further characterized the NMB0595 mutant and demonstrated that it had increased membrane permeability and was unable to form colonies on solid media with low magnesium concentrations, features that are consistent with disruption of PhoPQ-mediated modifications to the lipooligosaccharide structure. We examined the transcriptional profiles of wild-type and NMB0595 mutant strains and found that magnesium-regulated changes in gene expression are completely abrogated in the mutant, indicating that, similar to the salmonella PhoPQ system, the meningococcal PhoPQ system is regulated by magnesium. Transcriptional profiling of the mutant indicated that, also similar to the salmonella PhoPQ system, the meningococcal system is involved in control of virulence and remodeling of the bacterial cell surface in response to the host environment. The results are consistent with the hypothesis that the PhoP homologue plays a role in the meningococcus similar to the role played by PhoP in salmonella. Elucidating the role that the PhoPQ system and PhoPQ-regulated genes play in the response of the meningococcus to the host environment may provide new insights into the pathogenic process.
Abstract.
Author URL.
Charlie_Jeynes Details from cache as at 2019-06-15 18:14:39
Refresh publications
External Engagement and Impact
Editorial responsibilities
Reviewer for a number of journals including Nanotechnology, Chemosphere and PLOS one.
Invited lectures
June 2018:Conference talk at South West Structural Biology Conference 2018, Exeter“Dual-purpose gold nanoparticle probes for quantification in structural biology”
September 2017:Conference talk at Recent Appointments in Material Science Conference 2017, Exeter“Constructing quantitative X-ray fluorescence super resolution gold nanoparticle probes to measure biophysical properties of human chromosomes”
March 2017:Invited talk at Monte Carlo Multi-scale Modelling in Radiotherapy: From Ionisation to Clinical Impact, 2017 Manchester “Aligning experiments and models; commercial software symposium”
September 2016:Conference talk at BioDynamics Workshop 2016, Exeter“Measuring the length of human telomeres with XRF to inform cell ageing models”
July 2016:Conference talk at 16thIUBMB Conference, Vancouver, Canada “Getting more from QFISH: Correlation of Super Resolution Microscopy and X-ray Spectroscopy on nanoparticle probed telomeres”
June 2016:Invited talk at Department of Physics, National University of Singapore “Correlation of Super Resolution and X-ray Spectroscopy on nanoparticle probed telomeres”
September 2014:Invited talk at 8thInternational Symposium on BioPIXE, Slovenia “Measuring and modelling gold nanoparticles uptake in individual cancer cells with X-ray fluorescence”
Teaching
I am currently supervising with course lead James Wakefield, and Christian Soeller, a number of 3rd year undergraduate projects on the Natural Sciences degree.
PhD Students (PG researchers):
I am currently involved in a Doctoral Training Centre associated with CBMA and co-supervising a number of PhD students.
Supervision / Group
Postgraduate researchers
- I am currently involved in a Doctoral Training Centre associated with CBMA and co-supervising and helping a number of Ph.D students.
