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Dr Harley Worthy

Research Associate

 Biocatalysis Centre BC1.2

 

Biocatalysis Centre, University of Exeter, The Henry Wellcome Building for Biocatalysis, Stocker Road, Exeter, EX4 4QD , UK

 Office hours:

Monday – Friday 9 am – 5 pm

Overview

After graduating from Cardiff University with a B.Sc. Biochemistry I joined the lab of Dr Dafydd Jones for my Doctoral training. There I focussed on incorporating non-canonical amino acids (ncAAs) into proteins and studying the structural and functional changes of these mutations. I also studied how these mutations could be used to generate artificial protein oligomers and interface with nano materials and surfaces (DNA origami tiles and carbon nanotubes).

Currently, I am working in the lab of Prof, Littlechild in the Biocatalysis centre at University of Exeter, in conjunction with Unilever. My major focus on generating cold adaptive enzymes, for use in environmentally friendly biological washing formulations.

During my PhD and subsequent Post-doc positions in the Jones and Littlechild groups, I have developed expertise in multiple techniques including:

  • Cloning and DNA manipulation
  • Recombinant protein expression and purification
  • X-ray crystallography
  • Fluorescence/Absorbance spectroscopy
  • SEC-DLS
  • Protein modelling and molecular dynamics

Qualifications

PhD Biochemistry - Cardiff University (2014-2018)

B.Sc. (Hons) Biochemistry - Cardiff University (2010-2014)

Career

Post Doctoral Research Associate, University of Exeter (05/2019- Present)

Research Associate, Cardiff University (02/2018-05/2019)

 

Links

Research group links

Research

Publications

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Pope JR, Johnson RL, Jamieson WD, Worthy HL, Kailasam S, Ahmed RD, Taban I, Auhim HS, Watkins DW, Rizkallah PJ, et al (2020). Association of Fluorescent Protein Pairs and its Significant Impact on Fluorescence and Energy Transfer. Advanced Science, 2003167-2003167.
Worthy HL, Auhim HS, Jamieson WD, Pope JR, Wall A, Batchelor R, Johnson RL, Watkins DW, Rizkallah P, Castell OK, et al (2019). Author Correction: Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry (Communications Chemistry, (2019), 2, 1, (83), 10.1038/s42004-019-0185-5). Communications Chemistry, 2(1). Abstract.
Gwyther REA, Dafydd Jones D, Worthy HL (2019). Better together: Building protein oligomers naturally and by design. Biochemical Society Transactions, 47(6), 1773-1780. Abstract.  Full text.
Worthy HL, Auhim HS, Jamieson WD, Pope JR, Wall A, Batchelor R, Johnson RL, Watkins DW, Rizkallah P, Castell OK, et al (2019). Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry. Communications Chemistry, 2(1). Abstract.  Author URL.
Thomas SK, Jamieson WD, Gwyther REA, Bowen BJ, Beachey A, Worthy HL, Macdonald JE, Elliott M, Castell OK, Jones DD, et al (2019). Site-Specific Protein Photochemical Covalent Attachment to Carbon Nanotube Side Walls and its Electronic Impact on Single Molecule Function. Bioconjugate Chemistry, 31(3), 584-594. Full text.
Freeley M, Worthy HL, Ahmed R, Bowen B, Watkins D, Macdonald JE, Zheng M, Jones DD, Palma M (2017). Site-Specific One-to-One Click Coupling of Single Proteins to Individual Carbon Nanotubes: a Single-Molecule Approach. Journal of the American Chemical Society, 139(49), 17834-17840.
Halliwell LM, Jathoul AP, Bate JP, Worthy HL, Anderson JC, Jones DD, Murray JAH (2017). ΔFlucs: Brighter Photinus pyralis. firefly luciferases identified by surveying consecutive single amino acid deletion mutations in a thermostable variant. Biotechnology and Bioengineering, 115(1), 50-59.
Hartley AM, Worthy HL, Reddington SC, Rizkallah PJ, Jones DD (2016). Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle. Chem Sci, 7(10), 6484-6491. Abstract.  Author URL.

Publications by year


2020

Pope JR, Johnson RL, Jamieson WD, Worthy HL, Kailasam S, Ahmed RD, Taban I, Auhim HS, Watkins DW, Rizkallah PJ, et al (2020). Association of Fluorescent Protein Pairs and its Significant Impact on Fluorescence and Energy Transfer. Advanced Science, 2003167-2003167.

2019

Worthy HL, Auhim HS, Jamieson WD, Pope JR, Wall A, Batchelor R, Johnson RL, Watkins DW, Rizkallah P, Castell OK, et al (2019). Author Correction: Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry (Communications Chemistry, (2019), 2, 1, (83), 10.1038/s42004-019-0185-5). Communications Chemistry, 2(1). Abstract.
Gwyther REA, Dafydd Jones D, Worthy HL (2019). Better together: Building protein oligomers naturally and by design. Biochemical Society Transactions, 47(6), 1773-1780. Abstract.  Full text.
Worthy HL, Auhim HS, Jamieson WD, Pope JR, Wall A, Batchelor R, Johnson RL, Watkins DW, Rizkallah P, Castell OK, et al (2019). Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry. Communications Chemistry, 2(1). Abstract.  Author URL.
Thomas SK, Jamieson WD, Gwyther REA, Bowen BJ, Beachey A, Worthy HL, Macdonald JE, Elliott M, Castell OK, Jones DD, et al (2019). Site-Specific Protein Photochemical Covalent Attachment to Carbon Nanotube Side Walls and its Electronic Impact on Single Molecule Function. Bioconjugate Chemistry, 31(3), 584-594. Full text.

2017

Freeley M, Worthy HL, Ahmed R, Bowen B, Watkins D, Macdonald JE, Zheng M, Jones DD, Palma M (2017). Site-Specific One-to-One Click Coupling of Single Proteins to Individual Carbon Nanotubes: a Single-Molecule Approach. Journal of the American Chemical Society, 139(49), 17834-17840.
Halliwell LM, Jathoul AP, Bate JP, Worthy HL, Anderson JC, Jones DD, Murray JAH (2017). ΔFlucs: Brighter Photinus pyralis. firefly luciferases identified by surveying consecutive single amino acid deletion mutations in a thermostable variant. Biotechnology and Bioengineering, 115(1), 50-59.

2016

Hartley AM, Worthy HL, Reddington SC, Rizkallah PJ, Jones DD (2016). Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle. Chem Sci, 7(10), 6484-6491. Abstract.  Author URL.

Harley_Worthy Details from cache as at 2021-01-22 02:28:07

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