Publications by category
Journal articles
Wang P, Sheng H, Zheng K, Hong Y, Debnath SC, Yan C, Li K, Chen G, Xu J, Wu F, et al (2022). Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
Arch Microbiol,
204(7).
Abstract:
Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
A Gram-negative, aerobic, non-motile, oxidase-positive, catalase-positive, methyl red-positive, and lipase-negative bacterium, designated A5.8T, was isolated from beach sediment of Zhairuo Island located in the East China Sea. Growth occurred at 10-40 °C (optimum, 30 °C), pH 5.5-9.5 (optimum, 7.5), and 0-2% NaCl (optimum, 1.5%). Based on 16S rRNA gene sequence analysis, strain A5.8T belongs to the genus Ancylobacter, sharing the highest similarity with Ancylobacter aquaticus JCM 20518T (98.0%). Its polar lipids mainly consist of phosphatidylethanolamine (PE) and phosphatidylcholine (PC). The predominant fatty acids are summed feature 8 (C18:1ω7c and/or C18:1ω6c, 91.0%), and the major respiratory quinone is Q-10. The DNA G + C content is 67.2 mol%. Based on above analysis, as well as digital DNA-DNA hybridization (22.5-22.9%) and average nucleotide identity (83.0-83.6%) of strain A5.8T with reference type strains of the genus Ancylobacter, strain A5.8T was suggested to represent a novel species of the genus Ancylobacter, for which the name Ancylobacter gelatini sp. nov. is proposed. The type strain is A5.8T (= MCCC 1K07167T = LMG 32566T).
Abstract.
Author URL.
Sharifuzzaman SM, Rasid MH, Rubby IA, Debnath SC, Xing B, Chen G, Chowdhury MSN, Hossain MS (2021). DNA barcoding confirms a new record of flyingfish Cheilopogon spilonotopterus (Beloniformes: Exocoetidae) from the northern Bay of Bengal. Conservation Genetics Resources, 13(3), 323-328.
Khan I, Debnath SC, Yan C, Chen C, Xu Y, Wang W-J, Yu Y-C, Zheng D-Q, Xu J-Z, Wang P-M, et al (2020). Flavobacterium ajazii sp. nov. Isolated from Seaweed of Gouqi Island, China. Current Microbiology, 77(10), 2925-2932.
Debnath SC, Chen C, Khan I, Wang W-J, Zheng D-Q, Xu J-Z, Wang P-M (2020). Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
International Journal of Systematic and Evolutionary Microbiology,
70(7), 4250-4260.
Abstract:
Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12T) and sediment of Qinghai Lake (strain LaA7.5T), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus
.
. Flavobacterium
.
. showing the highest 16S rRNA sequence similarities to
.
. Flavobacterium arcticum
.
. SM1502T(97.6–97.7 %) and
.
. Flavobacterium suzhouense
.
. XIN-1T(96.5–96.6 %). Moreover, strains SaA2.12T and LaA7.5T showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C15 : 0, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12T and LaA7.5T showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12T and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5T and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12T and LaA7.5T were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12T represents a novel species with the name Flavobacterium salilacus sp. nov. with the type strain SaA2.12T (=KCTC 72220T=MCCC 1K03618T) and strain LaA7.5T as a subspecies within novel Flavobacterium salilacus with the name Flavobacterium salilacus subsp. altitudinum subsp. nov. with the type strain LaA7.5T (=KCTC 72806T=MCCC 1K04372T). These propositions automatically create Flavobacterium salilacus subsp. salilacus subsp. nov. with SaA2.12T (=KCTC 72220T=MCCC 1K03618T) as the type strain.
Abstract.
Khan I, Xie W-L, Yu Y-C, Sheng H, Xu Y, Wang J-Q, Debnath SC, Xu J-Z, Zheng D-Q, Ding W-J, et al (2020). Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes.
Marine Drugs,
18(12), 652-652.
Abstract:
Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A.
Abstract.
Debnath SC, Chen C, Liu S-X, Di Y-N, Zheng D-Q, Li X-Y, Xu X-W, Xu J-Z, Wang P-M (2019). Flavobacterium sharifuzzamanii sp. nov. Isolated from the Sediments of the East China Sea. Current Microbiology, 76(3), 297-303.
Debnath SC, Miyah AMA, Chen C, Sheng H, Xu X-W, Wu Y-H, Zheng D-Q, Xu J-Z, Di Y-N, Wang P-M, et al (2019). Flavobacterium zhairuonensis sp. nov. a gliding bacterium isolated from marine sediment of the East China Sea. Journal of Microbiology, 57(12), 1065-1072.
Chen C, Ye Y, Wang R, Zhang Y, Wu C, Debnath SC, Ma Z, Wang J, Wu M (2018). Streptomyces nigra sp. nov. Is a Novel Actinobacterium Isolated from Mangrove Soil and Exerts a Potent Antitumor Activity in Vitro. Frontiers in Microbiology, 9
Publications by year
2022
Wang P, Sheng H, Zheng K, Hong Y, Debnath SC, Yan C, Li K, Chen G, Xu J, Wu F, et al (2022). Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
Arch Microbiol,
204(7).
Abstract:
Ancylobacter gelatini sp. nov. isolated from beach sediment of Zhairuo Island, China.
A Gram-negative, aerobic, non-motile, oxidase-positive, catalase-positive, methyl red-positive, and lipase-negative bacterium, designated A5.8T, was isolated from beach sediment of Zhairuo Island located in the East China Sea. Growth occurred at 10-40 °C (optimum, 30 °C), pH 5.5-9.5 (optimum, 7.5), and 0-2% NaCl (optimum, 1.5%). Based on 16S rRNA gene sequence analysis, strain A5.8T belongs to the genus Ancylobacter, sharing the highest similarity with Ancylobacter aquaticus JCM 20518T (98.0%). Its polar lipids mainly consist of phosphatidylethanolamine (PE) and phosphatidylcholine (PC). The predominant fatty acids are summed feature 8 (C18:1ω7c and/or C18:1ω6c, 91.0%), and the major respiratory quinone is Q-10. The DNA G + C content is 67.2 mol%. Based on above analysis, as well as digital DNA-DNA hybridization (22.5-22.9%) and average nucleotide identity (83.0-83.6%) of strain A5.8T with reference type strains of the genus Ancylobacter, strain A5.8T was suggested to represent a novel species of the genus Ancylobacter, for which the name Ancylobacter gelatini sp. nov. is proposed. The type strain is A5.8T (= MCCC 1K07167T = LMG 32566T).
Abstract.
Author URL.
2021
Sharifuzzaman SM, Rasid MH, Rubby IA, Debnath SC, Xing B, Chen G, Chowdhury MSN, Hossain MS (2021). DNA barcoding confirms a new record of flyingfish Cheilopogon spilonotopterus (Beloniformes: Exocoetidae) from the northern Bay of Bengal. Conservation Genetics Resources, 13(3), 323-328.
2020
Khan I, Debnath SC, Yan C, Chen C, Xu Y, Wang W-J, Yu Y-C, Zheng D-Q, Xu J-Z, Wang P-M, et al (2020). Flavobacterium ajazii sp. nov. Isolated from Seaweed of Gouqi Island, China. Current Microbiology, 77(10), 2925-2932.
Debnath SC, Chen C, Khan I, Wang W-J, Zheng D-Q, Xu J-Z, Wang P-M (2020). Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
International Journal of Systematic and Evolutionary Microbiology,
70(7), 4250-4260.
Abstract:
Flavobacterium salilacus sp. nov. isolated from surface water of a hypersaline lake, and descriptions of Flavobacterium salilacus subsp. altitudinum subsp. nov. and Flavobacterium salilacus subsp. salilacus subsp. nov.
Two yellow-pigmented, Gram-stain-negative, aerobic, rod-shaped bacteria were isolated from the water of the hypersaline Chaka Salt Lake (strain SaA2.12T) and sediment of Qinghai Lake (strain LaA7.5T), PR China. According to the 16S rRNA phylogeny, the isolates belong to the genus
.
. Flavobacterium
.
. showing the highest 16S rRNA sequence similarities to
.
. Flavobacterium arcticum
.
. SM1502T(97.6–97.7 %) and
.
. Flavobacterium suzhouense
.
. XIN-1T(96.5–96.6 %). Moreover, strains SaA2.12T and LaA7.5T showed 99.73 % 16S rRNA sequence similarity to each other. Major fatty acids, respiratory quinones and polar lipids detected in these isolates were iso-C15 : 0, menaquinone-6 and phosphatidylethanolamine, respectively. Strains SaA2.12T and LaA7.5T showed significant unique characteristics between them as well as between the closest phylogenetic members. The highest digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SaA2.12T and its closest neighbours were 25.3 and 82.8 %, respectively; whereas these values (highest) between LaA7.5T and its closest members were 25.2 and 82.8 %, respectively. The dDDH and ANI values between strains SaA2.12T and LaA7.5T were calculated as 75.9 and 97.2 %, respectively. Therefore, based on polyphasic data, we propose that strain SaA2.12T represents a novel species with the name Flavobacterium salilacus sp. nov. with the type strain SaA2.12T (=KCTC 72220T=MCCC 1K03618T) and strain LaA7.5T as a subspecies within novel Flavobacterium salilacus with the name Flavobacterium salilacus subsp. altitudinum subsp. nov. with the type strain LaA7.5T (=KCTC 72806T=MCCC 1K04372T). These propositions automatically create Flavobacterium salilacus subsp. salilacus subsp. nov. with SaA2.12T (=KCTC 72220T=MCCC 1K03618T) as the type strain.
Abstract.
Khan I, Xie W-L, Yu Y-C, Sheng H, Xu Y, Wang J-Q, Debnath SC, Xu J-Z, Zheng D-Q, Ding W-J, et al (2020). Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes.
Marine Drugs,
18(12), 652-652.
Abstract:
Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A.
Abstract.
2019
Debnath SC, Chen C, Liu S-X, Di Y-N, Zheng D-Q, Li X-Y, Xu X-W, Xu J-Z, Wang P-M (2019). Flavobacterium sharifuzzamanii sp. nov. Isolated from the Sediments of the East China Sea. Current Microbiology, 76(3), 297-303.
Debnath SC, Miyah AMA, Chen C, Sheng H, Xu X-W, Wu Y-H, Zheng D-Q, Xu J-Z, Di Y-N, Wang P-M, et al (2019). Flavobacterium zhairuonensis sp. nov. a gliding bacterium isolated from marine sediment of the East China Sea. Journal of Microbiology, 57(12), 1065-1072.
2018
Chen C, Ye Y, Wang R, Zhang Y, Wu C, Debnath SC, Ma Z, Wang J, Wu M (2018). Streptomyces nigra sp. nov. Is a Novel Actinobacterium Isolated from Mangrove Soil and Exerts a Potent Antitumor Activity in Vitro. Frontiers in Microbiology, 9