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Dr Stefan Harmansa

Dr Stefan Harmansa

Senior Research Fellow

 2402

 Living Systems Institute T02.09

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

Morphomechanics – Shaping Growing Tissues

Morphogenesis delineates the complex process of shape generation during embryonic development. Morphogenesis is an intrinsically mechanical process where cellular activities, like contractility or growth, lead to stresses that mechanically mould tissues into their complex 3D shapes. Proper morphology is essential for organ functionality and defects in morphogenesis are linked to developmental disorders and disease.

I am particularly interested in the interplay between growing epithelial tissues and their basement membranes (BMs), specialised sheet-like extracellular matrices. Like the foundation of a building, BMs acts as base for epithelial cells and their mechanical properties (such as stiffness) as well as their growth properties directly influence cell and tissue morphology. I recently demonstrated that differential growth between a tissue and its BM leads to the accumulation of growth-induced mechanical stresses that guide tissue morphology (Harmansa et.al. 2023). Using a combination of Drosophila genetics, advanced imaging techniques, quantitative biophysical tools and data-informed modelling my group aims to uncover how such stresses arise during tissue growth and how they guide the mechanics of morphogenesis.

We are a newly established group that officially joined LSI in February 2024 and will be supported by a Wellcome Career Development Award.  For more details on our current research, news and open positions please visit our group’s webpage.

 

Principal Investigator Profile

Stefan studied Biology at the University of Basel, Switzerland. He then joined the team of Prof. Markus Affolter at the Biozentrum in Basel for his PhD work, studying organ size control during animal development. For his postdoctoral work he joined the team of Prof. Thomas Lecuit (Institute of Developmental Biology in Marseille, France) investigating how growth-induced stresses shape epithelial tissues. His work identified an essential role for the growth orientation of the extracellular matrix in determining 3-dimensional morphology during organ growth.

In February 2024 he joined the Living Systems Institute in Exeter (UK) as a group leader, investigating the biomechanical interplay between growing tissues and their basement membranes.

 

Qualifications

Degrees

2010 BSc, Integrative Biology, University of Basel (Switzerland)

2011 MSc, Molecular Biology, University of Basel (Switzerland)

2016 PhD (Hons., summa cum laude), Developmental Biology, University of Basel (Switzerland)

 

Awards

2023Career Development Award of the Wellcome Trust

2022 Prize of the Fondation Hugot of the Collège de France

2019CENTURI Postdoctoral Fellow

2017 – EMBO Long-Term Fellow

2015J.C.W. Shepherd Prize of the Biozentrum Basel

Career

Since 2024 Group leader at the Living Systems Institute, Bioscience, University of Exeter, UK

2017-2023 Postdoctoral Research Fellow with Prof. Thomas Lecuit, Institute of Developmental Biology Marseille (IBDM), Marseille, France

2011-2016 PhD student in Developmental Biology (Group of Prof. Markus Affolter), Biozentrum, University of Basel, Switzerland

Links

Research group links

Research

Research interests

Basement Membranes in Shape Generation

Organ shape emerges during morphogenesis, a mechanical process whereby forces exert stresses leading to cell and tissue deformation that shape growing tissues. The shaping of growing tissues depends on genetic cellular programs that biochemically and mechanically interact with the extracellular environment. In particular the extracellular matrix, a complex network of macromolecules surrounding the cells instructively guides morphogenesis.

We use the fruit fly Drosophila melanogaster to investigate how the Basement Membrane, a specialized extracellular matrix covering epithelial tissues, mechanically interacts with and thereby shapes the morphology of growing tissues. Basement Membranes (BM) are sheet-like extracellular matrices rich in Collagen IV. They cover the basal side of epithelial tissues, the major building blocks of our internal organs. Basement membranes provide physical support for tissues and their mechanical properties directly feed back on tissue morphology. We want to understand how growing tissues mechanically interact with their basement membranes and how this interaction is guiding changes in tissue shape.

Depending on their biophysical properties and dynamics growth patterns, basement membranes can act as geometric boundary and hinder the expansion of growing tissues. In particular, differences in growth dynamics can lead to the accumulation of residual stress which leads to cell and tissue-scale deformations. Using and interdisciplinary approach, we aim to uncover how such stresses emerge, with the ultimate goal to actively control such stresses and thereby guide custom tissue morphologies.

Publications

Key publications | Publications by category | Publications by year

Key publications


Harmansa S, Erlich A, Eloy C, Zurlo G, Lecuit T (2023). Growth anisotropy of the extracellular matrix shapes a developing organ. Nat Commun, 14(1). Abstract.  Author URL.
Harmansa S, Alborelli I, Bieli D, Caussinus E, Affolter M (2017). A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila. Elife, 6 Abstract.  Author URL.
Harmansa S, Hamaratoglu F, Affolter M, Caussinus E (2015). Dpp spreading is required for medial but not for lateral wing disc growth. Nature, 527(7578), 317-322. Abstract.  Author URL.

Publications by category


Journal articles

Harmansa S, Erlich A, Eloy C, Zurlo G, Lecuit T (2023). Growth anisotropy of the extracellular matrix shapes a developing organ. Nat Commun, 14(1). Abstract.  Author URL.
Lavalou J, Mao Q, Harmansa S, Kerridge S, Lellouch AC, Philippe J-M, Audebert S, Camoin L, Lecuit T (2021). Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry. Dev Cell, 56(11), 1574-1588.e7. Abstract.  Author URL.
Harmansa S, Lecuit T (2021). Forward and feedback control mechanisms of developmental tissue growth. Cells Dev, 168 Abstract.  Author URL.
Harmansa S, Affolter M (2018). Protein binders and their applications in developmental biology. Development, 145(2). Abstract.  Author URL.
Harmansa S, Alborelli I, Bieli D, Caussinus E, Affolter M (2017). A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila. Elife, 6 Abstract.  Author URL.
Ochoa-Espinosa A, Harmansa S, Caussinus E, Affolter M (2017). Myosin II is not required for Drosophila tracheal branch elongation and cell intercalation. Development, 144(16), 2961-2968. Abstract.  Author URL.
Matsuda S, Harmansa S, Affolter M (2016). BMP morphogen gradients in flies. Cytokine Growth Factor Rev, 27, 119-127. Abstract.  Author URL.
Bieli D, Alborelli I, Harmansa S, Matsuda S, Caussinus E, Affolter M (2016). Development and Application of Functionalized Protein Binders in Multicellular Organisms. Int Rev Cell Mol Biol, 325, 181-213. Abstract.  Author URL.
Harmansa S, Hamaratoglu F, Affolter M, Caussinus E (2015). Dpp spreading is required for medial but not for lateral wing disc growth. Nature, 527(7578), 317-322. Abstract.  Author URL.

Publications by year


2023

Harmansa S, Erlich A, Eloy C, Zurlo G, Lecuit T (2023). Growth anisotropy of the extracellular matrix shapes a developing organ. Nat Commun, 14(1). Abstract.  Author URL.

2021

Lavalou J, Mao Q, Harmansa S, Kerridge S, Lellouch AC, Philippe J-M, Audebert S, Camoin L, Lecuit T (2021). Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry. Dev Cell, 56(11), 1574-1588.e7. Abstract.  Author URL.
Harmansa S, Lecuit T (2021). Forward and feedback control mechanisms of developmental tissue growth. Cells Dev, 168 Abstract.  Author URL.

2018

Harmansa S, Affolter M (2018). Protein binders and their applications in developmental biology. Development, 145(2). Abstract.  Author URL.

2017

Harmansa S, Alborelli I, Bieli D, Caussinus E, Affolter M (2017). A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila. Elife, 6 Abstract.  Author URL.
Ochoa-Espinosa A, Harmansa S, Caussinus E, Affolter M (2017). Myosin II is not required for Drosophila tracheal branch elongation and cell intercalation. Development, 144(16), 2961-2968. Abstract.  Author URL.

2016

Matsuda S, Harmansa S, Affolter M (2016). BMP morphogen gradients in flies. Cytokine Growth Factor Rev, 27, 119-127. Abstract.  Author URL.
Bieli D, Alborelli I, Harmansa S, Matsuda S, Caussinus E, Affolter M (2016). Development and Application of Functionalized Protein Binders in Multicellular Organisms. Int Rev Cell Mol Biol, 325, 181-213. Abstract.  Author URL.

2015

Harmansa S, Hamaratoglu F, Affolter M, Caussinus E (2015). Dpp spreading is required for medial but not for lateral wing disc growth. Nature, 527(7578), 317-322. Abstract.  Author URL.

Stefan_Harmansa Details from cache as at 2024-03-01 16:09:05

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