Overview
I received my technical education and degree at the Ruprecht Karls University of Heidelberg, Germany, with particular focus on cell biology, anatomy and biochemistry. I obtained international experience at the Johns Hopkins University, USA and in Portugal. Besides academia, I also worked in a company with focus on drug development in cancer research. In 2012, I joined the University of Exeter and I am involved in several inter-disciplinary research projects to understand the dynamics and cooperation of subcellular compartments and their link to human disease. I have profound technical expertise in several research areas including molecular cell biology, biochemistry, tumour biology, and histology. Besides involvement in several research projects, I am managing the cell culture facility including our cell bank as well as general needs of the whole group.
Qualifications
1993–1997 Professional Education as Research Technician, University of Heidelberg, Germany
Professionally registered at the Industrie und Handelskammer, Baden-Wuerttemberg, Germany
Career
2017-ongoing Senior Research Technician, Biosciences, CLES, University of Exeter, UK
9/2014-03/2015 Laboratory Manager (Maternity cover), Biosciences, CLES, University of Exeter, UK
2012-2017 Research Technician, Biosciences, CLES, University of Exeter, UK
PREVIOUS APPOINTMENTS
2008-2011 Voluntary Research Technician, Centre for Cell Biology & Dept. of Biology, University of Aveiro, Portugal
06/2001-06/2004 Full Time Senior Research Technician, Vectron Therapeutics AG, Marburg, Germany
01/1999-05/2001 Full Time Research Technician, Institute for Molecular Biology and Tumour Research (IMT), Philipps-University, Marburg, Germany (Prof. Dr. R. Müller)
08/1997-10/1998 Voluntary Research Technician, Dept. of Biology, Johns Hopkins University, Baltimore, MD/USA (Prof. Dr. T. A. Schroer)
02/1997-04/1997 Full time Research Technician, Institute for Anatomy and Cell Biology II, University of Heidelberg, Germany (Profs. Dr. Dr. A. Völkl and H. D. Fahimi)
Links
Research group links
Research
Research projects
Involvement and support of research projects (2012 –ongoing)
2013-14 ISSF Strategic Seed Corn Fund, Wellcome Trust Institutional Strategic Support Award (WT097835MF), University of Exeter, UK
2013-14 Bridging the gaps “Super-resolution imaging of peroxisomes” with Prof C.Soeller, Physics, University of Exeter
2015 Cooperative international project “ Characterisation of Pex11 function” with the University of Groningen, The Netherlands; ongoing research on human Pex11
2015 Cooperative international project with the University of Aveiro, Portugal
2015-16 CLES Strategic Development Fund, CLES, University of Exeter, UK
2016 ISSF2 Strategic Seed Corn Fund, Wellcome Trust Institutional Strategic Support Award (WT105618MA), University of Exeter, UK (Project: Modelling of peroxisomal membrane dynamics in health and disease)
2016-19 Research Grant Application, BBSRC (BB/N01541X/1) (Project: Unveiling novel functions of peroxisomal lipid-binding proteins in interorganellar cooperation and regulation of lipid metabolism)
2017-18 Confidence in Concept Platform, MRC
2018-21 Research Grant Application, BBSRC (BB/R016844/1) (Project: Unveiling the molecular mechanisms to modulate peroxisome dynamics and abundance for improvement of cell performance)
Publications
Key publications | Publications by category | Publications by year
Publications by category
Journal articles
Schrader TA, Islinger M, Schrader M (In Press). Detection and immunolabelling of peroxisomal proteins. Methods in Molecular Biology
Costello J, Castro I, Schrader TA, Islinger M, Schrader M (In Press). Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations. Cell Cycle
Schrader TA, Schrader M (In Press). siRNA-mediated Silencing of Peroxisomal Genes in Mammalian Cells. Methods in Molecular Biology
Bishop A, Kamoshita M, Passmore J, Hacker C, Schrader T, Waterham H, Costello J, Schrader M (2019). Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells. Contact, 2
Castro IG, Richards DM, Metz J, Costello JL, Passmore JB, Schrader TA, Gouveia A, Ribeiro D, Schrader M (2018). A role for Mitochondrial Rho GTPase 1 (MIRO1) in motility and membrane dynamics of peroxisomes. Traffic, 19(3), 229-242.
Costello JL, Castro IG, Hacker C, Schrader TA, Metz J, Zeuschner D, Azadi AS, Godinho LF, Costina V, Findeisen P, et al (2017). ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER.
The Journal of Cell Biology,
216, 331-342.
Abstract:
ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER
Peroxisomes (POs) and the endoplasmic reticulum (ER) cooperate in cellular lipid metabolism and form tight structural associations, which were first observed in ultrastructural studies decades ago. PO-ER associations have been suggested to impact on a diverse number of physiological processes, including lipid metabolism, phospholipid exchange, metabolite transport, signaling, and PO biogenesis. Despite their fundamental importance to cell metabolism, the mechanisms by which regions of the ER become tethered to POs are unknown, in particular in mammalian cells. Here, we identify the PO membrane protein acyl-coenzyme A-binding domain protein 5 (ACBD5) as a binding partner for the resident ER protein vesicle-associated membrane protein-associated protein B (VAPB). We show that ACBD5-VAPB interaction regulates PO-ER associations. Moreover, we demonstrate that loss of PO-ER association perturbs PO membrane expansion and increases PO movement. Our findings reveal the first molecular mechanism for establishing PO-ER associations in mammalian cells and report a new function for ACBD5 in PO-ER tethering.
Abstract.
Costello JL, Castro IG, Cam oes F, Schrader TA, McNeall D, Yang J, Giannopoulou EA, Gomes S, Pogenberg V, Bonekamp NA, et al (2017). Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells.
Journal of Cell Science,
130, 1675-1687.
Abstract:
Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells
Tail-anchored (TA) proteins contain a single transmembrane domain (TMD) at the C-terminus that anchors them to the membranes of organelles where they mediate critical cellular processes. Accordingly, mutations in genes encoding TA proteins have been identified in a number of severe inherited disorders. Despite the importance of correctly targeting a TA protein to its appropriate membrane, the mechanisms and signals involved are not fully understood. In this study, we identify additional peroxisomal TA proteins, discover more proteins that are present on multiple organelles, and reveal that a combination of TMD hydrophobicity and tail charge determines targeting to distinct organelle locations in mammals. Specifically, an increase in tail charge can override a hydrophobic TMD signal and re-direct a protein from the ER to peroxisomes or mitochondria and vice versa. We show that subtle changes in those parameters can shift TA proteins between organelles, explaining why peroxisomes and mitochondria have many of the same TA proteins. This enabled us to associate characteristic physicochemical parameters in TA proteins with particular organelle groups. Using this classification allowed successful prediction of the location of uncharacterized TA proteins for the first time.
Abstract.
Schrader M, King SJ, Stroh TA, Schroer TA (2000). Real time imaging reveals a peroxisomal reticulum in living cells.
J Cell Sci,
113 ( Pt 20), 3663-3671.
Abstract:
Real time imaging reveals a peroxisomal reticulum in living cells.
We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.
Abstract.
Author URL.
Schrader M, Reuber BE, Morrell JC, Jimenez-Sanchez G, Obie C, Stroh TA, Valle D, Schroer TA, Gould SJ (1998). Expression of PEX11β Mediates Peroxisome Proliferation in the Absence of Extracellular Stimuli. Journal of Biological Chemistry, 273(45), 29607-29614.
Publications by year
In Press
Schrader TA, Islinger M, Schrader M (In Press). Detection and immunolabelling of peroxisomal proteins. Methods in Molecular Biology
Costello J, Castro I, Schrader TA, Islinger M, Schrader M (In Press). Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations. Cell Cycle
Schrader TA, Schrader M (In Press). siRNA-mediated Silencing of Peroxisomal Genes in Mammalian Cells. Methods in Molecular Biology
2019
Bishop A, Kamoshita M, Passmore J, Hacker C, Schrader T, Waterham H, Costello J, Schrader M (2019). Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells. Contact, 2
2018
Castro IG, Richards DM, Metz J, Costello JL, Passmore JB, Schrader TA, Gouveia A, Ribeiro D, Schrader M (2018). A role for Mitochondrial Rho GTPase 1 (MIRO1) in motility and membrane dynamics of peroxisomes. Traffic, 19(3), 229-242.
2017
Costello JL, Castro IG, Hacker C, Schrader TA, Metz J, Zeuschner D, Azadi AS, Godinho LF, Costina V, Findeisen P, et al (2017). ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER.
The Journal of Cell Biology,
216, 331-342.
Abstract:
ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER
Peroxisomes (POs) and the endoplasmic reticulum (ER) cooperate in cellular lipid metabolism and form tight structural associations, which were first observed in ultrastructural studies decades ago. PO-ER associations have been suggested to impact on a diverse number of physiological processes, including lipid metabolism, phospholipid exchange, metabolite transport, signaling, and PO biogenesis. Despite their fundamental importance to cell metabolism, the mechanisms by which regions of the ER become tethered to POs are unknown, in particular in mammalian cells. Here, we identify the PO membrane protein acyl-coenzyme A-binding domain protein 5 (ACBD5) as a binding partner for the resident ER protein vesicle-associated membrane protein-associated protein B (VAPB). We show that ACBD5-VAPB interaction regulates PO-ER associations. Moreover, we demonstrate that loss of PO-ER association perturbs PO membrane expansion and increases PO movement. Our findings reveal the first molecular mechanism for establishing PO-ER associations in mammalian cells and report a new function for ACBD5 in PO-ER tethering.
Abstract.
Costello JL, Castro IG, Cam oes F, Schrader TA, McNeall D, Yang J, Giannopoulou EA, Gomes S, Pogenberg V, Bonekamp NA, et al (2017). Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells.
Journal of Cell Science,
130, 1675-1687.
Abstract:
Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells
Tail-anchored (TA) proteins contain a single transmembrane domain (TMD) at the C-terminus that anchors them to the membranes of organelles where they mediate critical cellular processes. Accordingly, mutations in genes encoding TA proteins have been identified in a number of severe inherited disorders. Despite the importance of correctly targeting a TA protein to its appropriate membrane, the mechanisms and signals involved are not fully understood. In this study, we identify additional peroxisomal TA proteins, discover more proteins that are present on multiple organelles, and reveal that a combination of TMD hydrophobicity and tail charge determines targeting to distinct organelle locations in mammals. Specifically, an increase in tail charge can override a hydrophobic TMD signal and re-direct a protein from the ER to peroxisomes or mitochondria and vice versa. We show that subtle changes in those parameters can shift TA proteins between organelles, explaining why peroxisomes and mitochondria have many of the same TA proteins. This enabled us to associate characteristic physicochemical parameters in TA proteins with particular organelle groups. Using this classification allowed successful prediction of the location of uncharacterized TA proteins for the first time.
Abstract.
2000
Schrader M, King SJ, Stroh TA, Schroer TA (2000). Real time imaging reveals a peroxisomal reticulum in living cells.
J Cell Sci,
113 ( Pt 20), 3663-3671.
Abstract:
Real time imaging reveals a peroxisomal reticulum in living cells.
We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.
Abstract.
Author URL.
1998
Schrader M, Reuber BE, Morrell JC, Jimenez-Sanchez G, Obie C, Stroh TA, Valle D, Schroer TA, Gould SJ (1998). Expression of PEX11β Mediates Peroxisome Proliferation in the Absence of Extracellular Stimuli. Journal of Biological Chemistry, 273(45), 29607-29614.
Tina_Schrader Details from cache as at 2023-12-06 16:31:28
Refresh publications
External Engagement and Impact
Awards
Papin Prize Award (Highly Commended) at the Higher Education Technicians Summit 2017
http://blogs.exeter.ac.uk/technicalservices/2017/06/12/research-technician-wins-award-at-the-higher-education-technicians-summit-2017/)
Committee/panel activities
Member of the Technician Commitment Committee, University of Exeter
Conferences and invited presentations
- Health and Medical Research Showcase, Exeter, 2014
- IRLA Launch, London, 2014
- EU FP7 Marie Curie ITN PERFUME Workshop “Peroxisomes in Health and Disease”, 2014. Exeter, UK (involved in organisation)
- Technical Services Conference, Exeter, 2015
- Higher Education Technicians Summit, University of Warwick, 2017
- Technical Service Conference, Exeter, 2017
- Zellweger UK Charity, Patient-centred session, Exeter, 2017
- Technical Service Conference, Exeter, 2018
- ZUK Family Meeting, Devon, 2018
International recognition, such as international research collaborations, visiting research posts in overseas institutions, involvement at senior levels in international research associations, acting as referee for national and international research councils.
2015 – ongoing Visit of international partner laboratories for cooperative research
Media Coverage
Press Release “Pulling power reveals new insights into membrane dynamics in human cells“, Research News, University of Exeter, 25 January 2018,
http://www.exeter.ac.uk/research/newsandevents/news/title_635625_en.html
N. Hunt, Zellweger UK Charity (23.11.2017, BIO3086 Patient-centred session) http://www.zellweger.org.uk/index.php/news-2/lr-151655/
https://www.facebook.com/zellwegeruk/posts/1130082373792943
Technical Services news and events “Research technician wins award at the higher education technicians summit 2017“, University of Exeter, 12 June 2017,
http://blogs.exeter.ac.uk/technicalservices/2017/06/12/research-technician-wins-award-at-the-higher-education-technicians-summit-2017/
Press Release “Weather forecasting technology used to predict where proteins anchor within human cells“, Featured News, University of Exeter, 30 March 2017,
http://www.exeter.ac.uk/news/research/title_576535_en.html
Press Release “Breakthrough by Exeter cell biologists - We all need contacts – how organelles hug in cells“, Featured News, University of Exeter, 20 January 2017, http://www.exeter.ac.uk/news/featurednews/title_565173_en.html
Press Release “Scientists discover an additional function for the peroxisome protein Pex11p”, Research News, Biosciences, University of Exeter, 14 May 2015,
http://biosciences.exeter.ac.uk/news/featurednews/title_451624_en.html
Press Release “Prestigious European training network comes to Biosciences”, Research News, Biosciences, University of Exeter, 1 December 2014,
http://biosciences.exeter.ac.uk/news/featurednews/title_424671_en.html
Teaching
TRAINING ACTIVITIES AND SKILLS DEVELOPMENT
- Training of project students
- Instructor/training of master students, PhD students and Post-doctoral researchers
- Laboratory training of guest scientists, technical staff
- Work experience placements
- Participation in institutional seminars, weekly lab meetings and journal clubs
