Faculty of Health and Life Sciences

Professor Nick Smirnoff

Professor Nick Smirnoff

Professor of Plant Biochemistry

 N.Smirnoff@exeter.ac.uk

 5168

 01392 725168

 Geoffrey Pope MO4

 

Geoffrey Pope Building, University of Exeter , Stocker Road, Exeter, EX4 4QD, UK

Overview

My long term research interest is in the metabolism and functions of vitamin C (ascorbic acid) in plants. My laboratory has discovered the biosynthetic pathway of ascorbate and more recently we are focussing on control of its synthesis and accumulation in relation to light and its role in photoprotection of photosynthesis. I am more generally interested in the roles of antioxidants and reactive oxygen species. Biochemical and molecular genetics approaches are used, focussing on Arabidopsis thaliana as well as algae and cyanobacteria. I am director of the Exeter Mass Spectrometry Facility wihch focusses on small molecule analysis. My current research focuses on the following areas.

  • The functions of reactive oxygen species (ROS) and antioxidants in plants. Currently, the focus is on the metabolism and functions of ascorbate (vitamin C) and on the production and functions of hydrogen peroxide.
  • Plant and algal metabolism and metabolomics. We are developing LC-MS and GC-MS metabolite profiling methods and mass spectral databases for plants and algae. Imaging plant chemical composition and metabolism using Raman microscopy.
  • High temperature acclimation and adaptation in algae and cyanobacteria.

Qualifications

1981: PhD in Physiological Ecology, University of St Andrews
1978: BSc Botany, University of Bristol

Career

2006-present: Professor of Plant Biochemistry, School of Biosciences, University of Exeter
2001-2006: Reader in Plant Biochemistry, School of Biosciences, University of Exeter
1998-2001: Senior Lecturer, Department of Biological Sciences, University of Exeter
1984-1998: Lecturer, Department of Biological Sciences, University of Exeter
1982-1984: Birkbeck College, University of London
1984: Agricultural and Food Research Council Wain Fellow

Research group links

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Research

Research interests

The main focus is on the synthesis and function of vitamin C (ascorbic acid) and other antioxidants in plants, and the functions of reactive oxygen species (ROS). The lab also has wider interests in plant metabolism and responses to environmental stresses, particularly the involvement of ROS.

Research projects

The synthesis and function of vitamin C (ascorbate).

Ascorbate is a key vitamin in the human diet and plants are the main dietary source. Recent work has completed identification of all the genes and enzymes involved in the GDP-mannose (“Smirnoff-Wheeler”) pathway of ascorbate synthesis. This shows that the pathway is the major source of ascorbate and that it is essential for plant growth, as well as for stress resistance. Current research is aimed at understanding the functions of ascorbate and how its biosynthesis is controlled.

  • The control of ascorbate synthesis by light and its role in photoprotection.
  • The role of ascorbate and ascorbate oxidase in plant cell growth and development.
  • The role of ascorbate, along with other antioxidants and reactive oxygen species, in plant-pathogen interactions.

Physiological, biochemical, molecular and -omics (transcriptomics and metabolomics) approaches are being used to investigate these topics. The main experimental material is the ascorbate-deficient (vtc) mutants of the model plant Arabidopsis thaliana.

Plants and mammals employ different ascorbate biosynthesis pathways while fungi have an analogue (erythroascorbate). Biosynthetic pathways in protists have been little explored. Biochemical approaches and genome sequence information are being used to define biosynthetic pathways in a wide range of organisms with the aim of understanding the evolution and function(s) of ascorbate.

Imaging hydrogen peroxide production in plants using HyPer, a genetically-encoded YFP sensor (PDRA- Marino Exposito Rodriguez).

Understanding the role of hydrogen peroxide as a signalling molecule is hampered by inadequate methods to measure its production and concentration in specific subcellular compartments. In collaboration with Phil Mullineaux (Essex University) we are expressing the ratiometric YFP-based hydrogen peroxide sensor HyPer in plants. Along with a new pH sensor (pHRed, to correct for the pH sensitivity of HyPer)) and evaluating their use in measuring H2O2. By the end of the project, HyPer constructs targeted to multiple subcellular compartments will be provided to the research community.

Decreasing the oxygenase activity of Rubisco: a synthetic biology approach (PDRA- Chloe Singleton)

The oxygenase activity of the primary CO2 fixing enzyme Rubisco introduces inefficiency into photosynthesis giving rise to "wasteful" photorespiration (PR). Some plants and algae have evolved CO2 concentrating mechanisms (e.g. C4 photosynthesis, HCO3- transport) to increase [CO2]/[O2] at the Rubisco active site thus reducing PR. Because of decreased PR, C4 plants can have high productivity and require less water (stomata can be more closed) and nitrogen (less Rubisco is required). Cyanobacteria concentrate CO2 by packaging Rubisco and carbonic nanhydrase (CA) in protein microcompartments (carboxysomes). CA catalyses CO2 release from bicarbonate in the vicinity of Rubisco, thereby out-competing oxygenase. Although there has been speculation about transferring carboxysomes to plants their structural complexity makes this a formidable task. Also, introduction of C4 photosynthesis or production of Rubisco are possible approaches. We propose an alternative approach in which a protein scaffold tethers Rubisco and CA in close proximity. The scaffold is a synthetic polypeptide which contains concatenated protein-peptide interaction domains that will bind Rubisco and CA that have been tagged with the appropriate interaction peptides. This method has been used to produce a three enzyme metabolon that greatly improves local substrate concentration, resulting in greater flux through the mevalonate pathway in E. coli. Although the ultimate aim is to engineer plants, it is more convenient to carry out proof of concept in a cyanobacterium. The carboxysome-based CO2 concentrating mechanism of Synechocystis sp. PCC 6803 will be disrupted and the synthetic Rubisco/CA complex introduced. The effectiveness of the Rubisco/CA complex in reducing the oxygenase reaction will be assessed by measuring the photosynthetic characteristics of the cells. In parallel, the kinetic properties of the complex will be investigated in vitro.

Frequency-modulated stimulated Raman scattering microscopy for label-free functional imaging in planta (with Julian Moger, Physics Exeter).

Stimulated Raman Scattering (SRS) microscopy is an optical imaging technique that provides label-free chemically specific image contrast based on vibrational spectroscopy. Over the past decade it has been shown to have clear advantages for a vast range of biological applications. It provides quantitative functional analysis; does not suffer from photobleaching; allows near real time imaging; and has good depth penetration (several hundreds of microns) into scattering tissues. However, due to the susceptibility of current detection schemes to optical absorption from highly pigmented samples its application in plant science has remained virtually unexplored. This project aims to overcome the limitations of SRS for plant imaging by develop

The objectives of the project are:

i) To develop a new detection scheme for SRS microscopy, based on Frequency Modulation (FM-SRS), which is impervious to interference from pigments.

ii) To perform a short series of proof-of-principle investigations that demonstrate how label-free imaging with FM-SRS can be used to answer fundamental biological questions that are not possible with current analytical techniques.

Metabolite profiling and metabolism of planktonic microalgae (Debbie Salmon, PhD student).

We are assessing the use of NMR and LC-MS metabolite profiling to distinguish between genotypes of Emiliania huxleyi, a globally important phytoplankton species. The aim is to assess the genetic diversity of this species both geographically and within blooms and to understand the role of calcification.

Research networks

  • Takahiro Ishikawa (Shimane University, Japan)
  • Phil Mullineaux (University of Essex)
  • Alistair Hetherington (University of Bristol)
  • Glen Wheeler (Marine Biological Association, Plymouth)
  • Murray Grant (University of Warwick)
  • Julian Hibberd (University of Cambridge)
  • Patricia Conklin (State University of New York, Cortland)

Research grants

  • 2013 BBSRC
    Frequency-Modulated Stimulated Raman Scattering Microscopy for Label-Free Functional Imaging In-Planta
  • 2013 BBSRC
    Harnessing synthetic biology to improve photosynthesis: preparation of joint grant proposal for BBSRC-NSF photosynthesis initiative
  • 2011 BBSRC
    Decreasing the oxygenase activity of Rubisco: a synthetic biology approach
  • 2010 BBSRC
    Development of plant-based hydrogen peroxide YFP nanosensors targeted to multiple sub-cellular locations
  • 2010 EPSRC
    The Exeter Science Exchange:trading ideas to promote multi-disciplinary collaboration
  • 2009 BBSRC
    The control of ascorbate biosynthesis: the role of light and GDP-L-galactose phosphorylase
  • 2008 BBSRC
    Elucidating Signalling Networks in Plant Stress Responses. (Part of SABR consortium with Warwick and Essex Universities)
  • 2008 BBSRC
    Liquid chromatography-mass spectrometry equipment for plant metabolism and host-pathogen interactions (REI).
  • 2006 NERC
    GFP based biosensors for ascorbate and hormones
  • 2006 Leverhulme Trust
    GFP based biosensors for ascorbate and hormones
  • 2003 NERC
    Genomics of zinc hyperaccumulation in Arabidopsis halleri. (Environmental Genomics programme)
  • 2003 NERC
    Evolution of zinc hyperaccumulation.
  • 2001 BBSRC
    The role of RACs, ROS and antioxidants in cell growth: Arabidopsis root hairs as a model system.
  • 2001 BBSRC
    Metabolic profiling of sugars, sugar phosphates, nucleotide sugars and antioxidants in plants and fungi. (JREI equipment grant)

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Publications

Journal articles

Yvon-Durocher G, Buckling A, Smirnoff N (In Press). Adaptation of phytoplankton to a decade of experimental warming linked to increased photosynthesis. Nature Ecology and Evolution
Singleton C, Howard TP, Smirnoff N (In Press). Synthetic metabolons for metabolic engineering. Journal of Experimental Botany Abstract.
Smirnoff N, Wheeler GL (2024). The ascorbate biosynthesis pathway in plants is known but there is a way to go with understanding control and functions. J Exp Bot Abstract.  Author URL.
Arnaud D, Deeks MJ, Smirnoff N (2023). <scp>RBOHF</scp> activates stomatal immunity by modulating both reactive oxygen species and apoplastic <scp>pH</scp> dynamics in Arabidopsis. The Plant Journal, 116(2), 404-415. Abstract.
Barton S, Padfield D, Masterson A, Buckling A, Smirnoff N, Yvon‐Durocher G (2023). Comparative experimental evolution reveals species‐specific idiosyncrasies in marine phytoplankton adaptation to warming. Global Change Biology, 29(18), 5261-5275. Abstract.
Lamanchai K, Smirnoff N, Salmon DL, Ngernmuen A, Roytrakul S, Leetanasaksakul K, Kittisenachai S, Jantasuriyarat C (2022). <i>OsVTC1-1</i> Gene Silencing Promotes a Defense Response in Rice and Enhances Resistance to <i>Magnaporthe oryzae</i>. PLANTS-BASEL, 11(17).  Author URL.
Lamanchai K, Salmon DL, Smirnoff N, Sutthinon P, Roytrakul S, Leetanasaksakul K, Kittisenachai S, Jantasuriyarat C (2022). <i>OsVTC1-1</i> RNAi Mutant with Reduction of Ascorbic Acid Synthesis Alters Cell Wall Sugar Composition and Cell Wall-Associated Proteins. AGRONOMY-BASEL, 12(6).  Author URL.
Schaum C-E, Buckling A, Smirnoff N, Yvon-Durocher G (2022). Evolution of thermal tolerance and phenotypic plasticity under rapid and slow temperature fluctuations. Proc Biol Sci, 289(1980). Abstract.  Author URL.
Arnaud D, Deeks MJ, Smirnoff N (2022). Organelle-targeted biosensors reveal distinct oxidative events during pattern-triggered immune responses. Plant Physiology, 191(4), 2551-2569. Abstract.
Hancock RD, Smirnoff N, Lunn JE (2021). <i>Journal of Experimental Botany</i> 70th anniversary: plant metabolism in a changing world. Journal of Experimental Botany, 72(17), 5939-5941.
Littlejohn GR, Breen S, Smirnoff N, Grant M (2021). Chloroplast immunity illuminated. New Phytol, 229(6), 3088-3107. Abstract.  Author URL.
Arnaud D, Deeks MJ, Smirnoff N (2021). Differences between apoplastic and cytosolic reactive oxygen species production in<i>Arabidopsis</i>during pattern-triggered immunity. Abstract.
Bömer M, Pérez-Salamó I, Florance HV, Salmon D, Dudenhoffer J-H, Finch P, Cinar A, Smirnoff N, Harvey A, Devoto A, et al (2021). Jasmonates induce Arabidopsis bioactivities selectively inhibiting the growth of breast cancer cells through CDC6 and mTOR. New Phytol, 229(4), 2120-2134. Abstract.  Author URL.
Feroz H, Ferlez B, Oh H, Mohammadiarani H, Ren T, Baker CS, Gajewski JP, Lugar DJ, Gaudana SB, Butler P, et al (2021). Liposome-based measurement of light-driven chloride transport kinetics of halorhodopsin. Biochimica et Biophysica Acta - Biomembranes, 1863(8). Abstract.
Xiong H, Hua L, Reyna-Llorens I, Shi Y, Chen K-M, Smirnoff N, Kromdijk J, Hibberd JM (2021). Photosynthesis-independent production of reactive oxygen species in the rice bundle sheath during high light is mediated by NADPH oxidase. Proc Natl Acad Sci U S A, 118(25). Abstract.  Author URL.
Geigenberger P, Smirnoff N, Van Breusegem F, Dietz K-J, Noctor G (2021). Plant redox biology—on the move. Plant Physiology, 186(1), 1-3.
Helliwell KE, Kleiner FH, Hardstaff H, Chrachri A, Gaikwad T, Salmon D, Smirnoff N, Wheeler GL, Brownlee C (2021). Spatiotemporal patterns of intracellular Ca<sup>2+</sup> signalling govern hypo‐osmotic stress resilience in marine diatoms. New Phytologist, 230(1), 155-170. Abstract.
Fenech M, Amorim-Silva V, Esteban del Valle A, Arnaud D, Ruiz-Lopez N, Castillo AG, Smirnoff N, Botella MA (2021). The role of GDP-<scp>l</scp>-galactose phosphorylase in the control of ascorbate biosynthesis. Plant Physiology, 185(4), 1574-1594. Abstract.
Alegre ML, Steelheart C, Baldet P, Rothan C, Just D, Okabe Y, Ezura H, Smirnoff N, Gergoff Grozeff GE, Bartoli CG, et al (2020). Deficiency of GDP-L-galactose phosphorylase, an enzyme required for ascorbic acid synthesis, reduces tomato fruit yield. Planta, 251(2). Abstract.  Author URL.
Barton S, Jenkins J, Buckling A, Schaum C-E, Smirnoff N, Raven JA, Yvon‐Durocher G (2020). Evolutionary temperature compensation of carbon fixation in marine phytoplankton. Ecology Letters, 23(4), 722-733. Abstract.
Fenech M, Amorim-Silva V, del Valle AE, Arnaud D, Castillo AG, Smirnoff N, Botella MA (2020). Organization and control of the ascorbate biosynthesis pathway in plants. Abstract.
Dewhirst RA, Smirnoff N, Belcher CM (2020). Pine species that support crown fire regimes have lower leaf-level terpene contents than those native to surface fire regimes. Fire, 3(2), 1-19. Abstract.
Bunbury F, Helliwell KE, Mehrshahi P, Davey MP, Salmon DL, Holzer A, Smirnoff N, Smith AG (2020). Responses of a Newly Evolved Auxotroph of Chlamydomonas to B12 Deprivation. Plant Physiol, 183(1), 167-178. Abstract.  Author URL.
Haque T, Eaves DJ, Lin Z, Zampronio CG, Cooper HJ, Bosch M, Smirnoff N, Franklin-Tong VE (2020). Self-Incompatibility Triggers Irreversible Oxidative Modification of Proteins in Incompatible Pollen. Plant Physiol, 183(3), 1391-1404. Abstract.  Author URL.
Mullineaux PM, Exposito-Rodriguez M, Laissue PP, Smirnoff N, Park E (2020). Spatial chloroplast-to-nucleus signalling involving plastid-nuclear complexes and stromules. Philos Trans R Soc Lond B Biol Sci, 375(1801). Abstract.  Author URL.
Drenichev MS, Bennett M, Novikov RA, Mansfield J, Smirnoff N, Grant M, Mikhailov SN (2019). A role for 3′-O-β-D-ribofuranosyladenosine in altering plant immunity. Phytochemistry, 157, 128-134. Abstract.
Smirnoff N (2019). Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes. Plant Physiol, 179(3), 918-928. Abstract.  Author URL.
Smirnoff N, Arnaud D (2019). Hydrogen peroxide metabolism and functions in plants. New Phytol, 221(3), 1197-1214. Abstract.  Author URL.
Walker CE, Heath S, Salmon DL, Smirnoff N, Langer G, Taylor AR, Brownlee C, Wheeler GL (2018). An extracellular polysaccharide-rich organic layer contributes to organization of the coccosphere in coccolithophores. Frontiers in Marine Science, 5(AUG). Abstract.
Smirnoff N (2018). Ascorbic acid metabolism and functions: a comparison of plants and mammals. Free Radical Biology and Medicine
Schaum E, Buckling A, Studholme D, Smirnoff N, Yvon-Durocher G (2018). Environmental fluctuations accelerate molecular evolution of thermal tolerance in a marine diatom. Nature Communications
Schaum C-E, Buckling A, Smirnoff N, Studholme DJ, Yvon-Durocher G (2018). Environmental fluctuations accelerate molecular evolution of thermal tolerance in a marine diatom (vol 9, 1719, 2018). NATURE COMMUNICATIONS, 9  Author URL.
Feroz H, Ferlez B, Lefoulon C, Ren T, Baker CS, Gajewski JP, Lugar DJ, Gaudana SB, Butler PJ, Hühn J, et al (2018). Light-Driven Chloride Transport Kinetics of Halorhodopsin. Biophysical Journal, 115(2), 353-360. Abstract.
Helliwell KE, Pandhal J, Cooper MB, Longworth J, Kudahl UJ, Russo DA, Tomsett EV, Bunbury F, Salmon DL, Smirnoff N, et al (2018). Quantitative proteomics of a B12 -dependent alga grown in coculture with bacteria reveals metabolic tradeoffs required for mutualism. New Phytol, 217(2), 599-612. Abstract.  Author URL.
Mullineaux PM, Exposito-Rodriguez M, Laissue PP, Smirnoff N (2018). ROS-dependent signalling pathways in plants and algae exposed to high light: Comparisons with other eukaryotes. Free Radic Biol Med, 122, 52-64. Abstract.  Author URL.
Barton S, Jenkins J, Buckling A, Schaum C-E, Smirnoff N, Yvon-Durocher G (2018). Universal metabolic constraints on the thermal tolerance of marine phytoplankton. Abstract.
Sambles CM, Salmon DL, Florance H, Howard TP, Smirnoff N, Nielsen LR, McKinney LV, Kjær ED, Buggs RJA, Studholme DJ, et al (2017). Ash leaf metabolomes reveal differences between trees tolerant and susceptible to ash dieback disease. Sci Data, 4 Abstract.  Author URL.
Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, Mullineaux PM (2017). Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nature Communications, 8(1). Abstract.
Lim B, Smirnoff N, Cobbett CS, Goltz JF (2016). Ascorbate-Deficient vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth. Frontiers in Plant Science, 7, 1025-1025.
de Torres Zabala M, Zhai B, Jayaraman S, Eleftheriadou G, Winsbury R, Yang R, Truman W, Tang S, Smirnoff N, Grant M, et al (2016). Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection. New Phytologist, 209(3), 1120-1134. Abstract.
de Torres Zabala M, Zhai B, Jayaraman S, Eleftheriadou G, Winsbury R, Yang R, Truman W, Tang S, Smirnoff N, Grant M, et al (2016). Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection. New Phytol, 209(3), 1120-1134. Abstract.  Author URL.
Bechtold U, Penfold CA, Jenkins DJ, Legaie R, Moore JD, Lawson T, Matthews JSA, Vialet-Chabrand SRM, Baxter L, Subramaniam S, et al (2016). Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis. Plant Cell, 28(2), 345-366. Abstract.  Author URL.
Goodey NA, Florance HV, Smirnoff N, Hodgson DJ (2015). Aphids Pick Their Poison: Selective Sequestration of Plant Chemicals Affects Host Plant Use in a Specialist Herbivore. J Chem Ecol, 41(10), 956-964. Abstract.  Author URL.
Sultana N, Florance HV, Johns A, Smirnoff N (2015). Ascorbate deficiency influences the leaf cell wall glycoproteome in Arabidopsis thaliana. Plant Cell and Environment, 38(2), 375-384. Abstract.
de Torres M, Littlejohn G, Jayaraman S, Studholme D, Bailey TC, Lawson T, Delfino L, Licht D, Truman W, Bölter B, et al (2015). Chloroplasts play a central role in plant defence and are targeted by pathogen effectors. Nature Plants, 1, n/a-n/a.
Wheeler GL, Smirnoff N, Ishikawa T (2015). EVOLUTION OF ALTERNATIVE PATHWAYS FOR VITAMIN C SYNTHESIS FOLLOWING PLASTID ACQUISITION. EUROPEAN JOURNAL OF PHYCOLOGY, 50, 52-52.  Author URL.
Wheeler G, Ishikawa T, Pornsaksit V, Smirnoff N (2015). Evolution of alternative biosynthetic pathways for vitamin C following plastid acquisition in photosynthetic eukaryotes. Elife, 4 Abstract.  Author URL.
Littlejohn GR, Mansfield JC, Parker D, Lind R, Perfect S, Seymour M, Smirnoff N, Love J, Moger J (2015). In vivo chemical and structural analysis of plant cuticular waxes using stimulated Raman scattering microscopy. Plant Physiol, 168(1), 18-28. Abstract.  Author URL.
Proctor MCF, Smirnoff N (2015). Photoprotection in bryophytes: Rate and extent of dark relaxation of non-photochemical quenching of chlorophyll fluorescence. Journal of Bryology, 37(3), 171-177. Abstract.
MacGregor DR, Kendall SL, Florance H, Fedi F, Moore K, Paszkiewicz K, Smirnoff N, Penfield S (2015). Seed production temperature regulation of primary dormancy occurs through control of seed coat phenylpropanoid metabolism. New Phytol, 205(2), 642-652. Abstract.  Author URL.
Lewis LA, Polanski K, de Torres-Zabala M, Jayaraman S, Bowden L, Moore J, Penfold CA, Jenkins DJ, Hill C, Baxter L, et al (2015). Transcriptional Dynamics Driving MAMP-Triggered Immunity and Pathogen Effector-Mediated Immunosuppression in Arabidopsis Leaves Following Infection with Pseudomonas syringae pv tomato DC3000. Plant Cell, 27(11), 3038-3064. Abstract.  Author URL.
Littlejohn GR, Mansfield JC, Christmas JT, Witterick E, Fricker MD, Grant MR, Smirnoff N, Everson RM, Moger J, Love J, et al (2014). An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology. Front Plant Sci, 5 Abstract.  Author URL.
Cresswell JE, Robert FXL, Florance H, Smirnoff N (2014). Clearance of ingested neonicotinoid pesticide (imidacloprid) in honey bees (Apis mellifera) and bumblebees (Bombus terrestris). Pest Management Science, 70(2), 332-337. Abstract.
Cresswell JE, Robert F-XL, Florance H, Smirnoff N (2014). Clearance of ingested neonicotinoid pesticide (imidacloprid) in honey bees (Apis mellifera) and bumblebees (Bombus terrestris). Pest Manag Sci, 70(2), 332-337. Abstract.  Author URL.
Chen M, MacGregor DR, Dave A, Florance H, Moore K, Paszkiewicz K, Smirnoff N, Graham IA, Penfield S (2014). Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year. Proc Natl Acad Sci U S A, 111(52), 18787-18792. Abstract.  Author URL.
Madadkar Haghjou M, Colville L, Smirnoff N (2014). The induction of menadione stress tolerance in the marine microalga, Dunaliella viridis, through cold pretreatment and modulation of the ascorbate and glutathione pools. Plant Physiol Biochem, 84, 96-104. Abstract.  Author URL.
Leonelli S, Smirnoff, Nicholas, Moore, Jonathan, Cook, Charis, Bastow, Ruth (2013). Making Open Data Work for Plant Science. Journal of Experimental Botany, 64(14), 4109-4117.
Howard TP, Middelhaufe S, Moore K, Edner C, Kolak DM, Taylor GN, Parker DA, Lee R, Smirnoff N, Aves SJ, et al (2013). Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli. Proc Natl Acad Sci USA, 110, 7636-7641. Abstract.  Author URL.
Exposito-Rodriguez M, Laissue PP, Littlejohn GR, Smirnoff N, Mullineaux PM (2013). The use of HyPer to examine spatial and temporal changes in H2O2 in high light-exposed plants. Methods Enzymol, 527, 185-201. Abstract.  Author URL.
Perera V, de Torres Zabala M, Florance H, Smirnoff N, Grant M, Yang ZR (2012). Aligning extracted LC-MS peak lists via density maximization. Metabolomics, 8, 175-185. Abstract.
Cresswell JE, Page CJ, Uygun MB, Holmbergh M, Li Y, Wheeler JG, Laycock I, Pook CJ, de Ibarra NH, Smirnoff N, et al (2012). Differential sensitivity of honey bees and bumble bees to a dietary insecticide (imidacloprid). Zoology, 115(6), 365-371. Abstract.
Smirnoff N (2012). THE ROLE OF ASCORBATE IN THE ACCLIMATION OF LEAVES TO HIGH LIGHT. PHARMACEUTICAL BIOLOGY, 50(5), 634-634.  Author URL.
Page M, Sultana N, Paszkiewicz K, Florance H, Smirnoff N (2012). The influence of ascorbate on anthocyanin accumulation during high light acclimation in Arabidopsis thaliana: further evidence for redox control of anthocyanin synthesis. Plant, Cell & Environment, 35, 388-404. Abstract.
Perera V, De Torres Zabala M, Florance H, Smirnoff N, Grant M, Yang ZR (2011). Aligning extracted LC-MS peak lists via density maximization. Metabolomics, 1-11.
Proctor MCF, Smirnoff N (2011). Ecophysiology of photosynthesis in bryophytes: major roles for oxygen photoreduction and non-photochemical quenching?. Physiol Plant, 141(2), 130-140. Abstract.  Author URL.
Gao Y, Badejo AA, Shibata H, Sawa Y, Maruta T, Shigeoka S, Page M, Smirnoff N, Ishikawa T (2011). Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana. Biosci Biotechnol Biochem, 75(9), 1783-1788. Abstract.  Author URL.
Gao Y, Nishikawa H, Badejo AA, Shibata H, Sawa Y, Nakagawa T, Maruta T, Shigeoka S, Smirnoff N, Ishikawa T, et al (2011). Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana. J Exp Bot, 62(10), 3647-3657. Abstract.  Author URL.
Salmon D, Smirnoff N, Wheeler G, Brownlee C (2011). METABOLITE PROFILING INDICATES INTRASPECIFIC VARIABILITY IN THE ACCUMULATION OF COMPATIBLE SOLUTES IN THE HAPTOPHYTE ALGA <i>EMILIANIA HUXLEYI</i>. EUROPEAN JOURNAL OF PHYCOLOGY, 46, 154-155.  Author URL.
Wilkins KA, Bancroft J, Bosch M, Ings J, Smirnoff N, Franklin-Tong VE (2011). Reactive oxygen species and nitric oxide mediate actin reorganization and programmed cell death in the self-incompatibility response of papaver. Plant Physiol, 156(1), 404-416. Abstract.  Author URL.
Smirnoff N (2011). Vitamin C: the metabolism and functions of ascorbic acid in plants. , 59, 107-177. Abstract.
Littlejohn GR, Gouveia JD, Edner C, Smirnoff N, Love J (2010). Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll. New Phytol, 186(4), 1018-1025. Abstract.  Author URL.
Smirnoff N (2010). Tocochromanols: rancid lipids, seed longevity, and beyond. Proc Natl Acad Sci U S A, 107(42), 17857-17858.  Author URL.
Spelman K, Iiams-Hauser K, Cech NB, Taylor EW, Smirnoff N, Wenner CA (2009). Role for PPARgamma in IL-2 inhibition in T cells by Echinacea-derived undeca-2E-ene-8,10-diynoic acid isobutylamide. Int Immunopharmacol, 9(11), 1260-1264. Abstract.  Author URL.
Haghjou MM, Shariati M, Smirnoff N (2009). The effect of acute high light and low temperature stresses on the ascorbate-glutathione cycle and superoxide dismutase activity in two Dunaliella salina strains. Physiol Plant, 135(3), 272-280. Abstract.  Author URL.
Galvez-Valdivieso G, Fryer MJ, Lawson T, Slattery K, Truman W, Smirnoff N, Asami T, Davies WJ, Jones AM, Baker NR, et al (2009). The high light response in Arabidopsis involves ABA signaling between vascular and bundle sheath cells. Plant Cell, 21(7), 2143-2162. Abstract.  Author URL.
Colville L, Smirnoff N (2008). Antioxidant status, peroxidase activity, and PR protein transcript levels in ascorbate-deficient Arabidopsis thaliana vtc mutants. J Exp Bot, 59(14), 3857-3868. Abstract.  Author URL.
Smirnoff N, Grant M (2008). Plant biology: do DELLAs do defence?. Curr Biol, 18(14), R617-R619. Abstract.  Author URL.
Dowdle J, Ishikawa T, Gatzek S, Rolinski S, Smirnoff N (2008). Two genes in <i>Arabidopsis thaliana</i> encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability (vol 52, pg 673, 2007). PLANT JOURNAL, 53(3), 595-595.  Author URL.
Macnair MR, Dowdle J, Filatov V, Ford-Lloyd B (2007). A quantitative trait loci analysis of zinc hyperaccumulation in Arabidopsis halleri. New Phytologist, 174(3), 580-590.
Smirnoff N (2007). Antioxidants and Reactive Oxygen Species in Plants. Antioxidants and Reactive Oxygen Species in Plants, 1-302. Abstract.
vanderHoning, H. Nishioka, M. Uehara, Y. (2007). Armadillo repeat-containing kinesins and a NIMA-related kinase are required for epidermal-cell morphogenesis in Arabidopsis. Plant Journal, 10.1111/j.1365-313X.
Smirnoff N (2007). Ascorbate, Tocopherol and Carotenoids: Metabolism, Pathway Engineering and Functions. , 53-86.
Ishikawa T, Dowdle J, Smirnoff N (2007). Erratum: Progress in manipulating ascorbic acid biosynthesis and accumulation in plants (Physiologia Plantarum (2006) 126, (343-355)). Physiologia Plantarum, 129(4).
Talbot NJ, Egan MJ, Jones MA, Smirnoff N (2007). Generation of reactive oxygen species by fungal NADPH oxidases is required for rice blast disease. Proceedings of the National Academy of Sciences, 104(28), 11772-11777.
Raymond, M.J. Yang, Z. Smirnoff, N. (2007). NADPH oxidase-dependent reactive oxygen species formation required for root-hair growth depends on ROP GTPase. Journal of Experimental Botany, 58, 1261-1270.
Smirnoff N (2007). Preface. Antioxidants and Reactive Oxygen Species in Plants
Ishikawa T, Dowdle J, Smirnoff N (2007). Progress in manipulating ascorbic acid biosynthesis and accumulation in plants (vol 126, pg 343, 2006). PHYSIOLOGIA PLANTARUM, 129(4), 831-831.  Author URL.
Jones MA, Smirnoff N (2007). Reactive Oxygen Species in Plant Development and Pathogen Defence. , 197-214.
Jones, M.A. Bezvoda, R. Smirnoff, N. (2007). Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth. New Phytologist, 174, 742-751.
Potocky M, Zarsky V, Jones M, Smirnoff N, Bezvoda R (2007). Reactive oxygen species produced by NADPH oxidase are involved in pollen tube growth. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 146(4), S269-S270.  Author URL.
Smirnoff N, Dowdle J, Ishikawa T (2007). The role of VTC2 in vitamin C biosynthesis in <i>Arabidopsis thaliana</i>. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 146(4), S250-S250.  Author URL.
Ishikawa, T. Gatzek, S. Rolinski, S. (2007). Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability. Plant Journal, 52, 673-689.
Smirnoff N, Jones MA, Raymond MJ (2006). Analysis of the root-hair morphogenesis transcriptome reveals the molecular identity of six genes with roles in root hair development in Arabidopsis . The Plant Journal, 45(1), 83-100.
Macnair MR, Dowdle J, Ford-Lloyd B, Smirnoff N (2006). Comparison of gene expression in segregating families identifies genes and genomic regions involved in a novel adaptation, zinc hyperaccumulation. Molecular Ecology, 15(10), 3045-3059.
Hancock JT, Smirnoff N, Foyer CH (2006). Journal of Experimental Botany: Preface. Journal of Experimental Botany, 57(8).
Smirnoff, N. (2006). Nuclear dynamics during the simultaneous and sustained tip growth of multiple root hairs arising from a single root epidermal cell. Journal of Experimental Botany, 57, 4269-4275.
Hancock JT, Smirnoff N, Foyer CH (2006). Oxygen metabolism coming up ROSes: a holistic view of the redox metabolism of plant cells - Preface. JOURNAL OF EXPERIMENTAL BOTANY, 57(8), IV-V.  Author URL.
Dowdle, J. Smirnoff, N. (2006). Progress in manipulating ascorbic acid biosynthesis and accumulation in plants. Physiologia Plantarum, 125, 343-355.
Smirnoff N, Conklin PL, Gatzek S, Ishikawa T (2006). The Arabidopsis thaliana VTC4 gene encodes L-galactose-1-P  phosphatase, a plant ascorbic acid biosynthetic enzyme. Journal of Biological Chemistry, 281(23), 15662-15670.
Baroja-Mazo A, Del Valle P, Rúa J, De Cima S, Busto F, De Arriaga D, Smirnoff N (2005). Characterisation and biosynthesis of D-erythroascorbic acid in Phycomyces blakesleeanus. Fungal Genetics and Biology, 42(5), 390-402. Abstract.
Baroja-Mazo A, del Valle P, Rúa J, de Cima S, Busto F, de Arriaga D, Smirnoff N (2005). Characterisation and biosynthesis of D-erythroascorbic acid in Phycomyces blakesleeanus. Fungal Genet Biol, 42(5), 390-402. Abstract.  Author URL.
Burrell M, Smirnoff N (2005). Making sense of the metabolome special issue - Preface. JOURNAL OF EXPERIMENTAL BOTANY, 56(410), IV-IV.  Author URL.
Burrell M, Smirnoff N (2005). Making sense of the metabolome: Preface. Journal of Experimental Botany, 56(410).
Smirnoff N (2003). Vitamin C booster. Nat Biotechnol, 21(2), 134-136.  Author URL.
Smirnoff N, Gatzek S, Wheeler GL (2002). Antisense suppression of L-galactose dehydrogenase in Arabidopsis thaliana provides evidence for its role in ascorbate synthesis and reveals light modulated L-galactose synthesis. The Plant Journal, 30(5), 541-553.
Smirnoff N (2002). Journal of Experimental Botany: Preface. Journal of Experimental Botany, 53(372).
Smirnoff N (2002). Plants in cold climates and waterlogged soils. Annals of Botany, 90(4).
Raymond MJ, Smirnoff N (2002). Proline metabolism and transport in maize seedlings at low water potential. Ann Bot, 89 Spec No(7), 813-823. Abstract.  Author URL.
Murakeözy EP, Smirnoff N, Nagy Z, Tuba Z (2002). Seasonal accumulation pattern of pinitol and other carbohydrates in Limonium gmelini subsp. hungarica. Journal of Plant Physiology, 159(5), 485-490. Abstract.
Smirnoff N (2002). Untitled - Preface. JOURNAL OF EXPERIMENTAL BOTANY, 53(372).  Author URL.
Smirnoff N, Conklin PL, Loewus FA (2001). Biosynthesis of ascorbic acid in plants: a renaissance. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 52, 437-+.  Author URL.
Smirnoff N (2001). L-ascorbic acid biosynthesis. Vitam Horm, 61, 241-266. Abstract.  Author URL.
Bachmann, K. Smirnoff, N. Macnair, M.R. (2001). The role of drought tolerance in serpentine tolerance in the Mimulus guttatus Fischer ex DC. complex. South African Journal of Science, 97, 581-586.
Smirnoff N, Wheeler GL (2000). Ascorbic acid in plants: Biosynthesis and function. Critical Reviews in Plant Sciences, 19(4), 267-290. Abstract.
Smirnoff N, Wheeler GL (2000). Ascorbic acid in plants: biosynthesis and function. Crit Rev Biochem Mol Biol, 35(4), 291-314. Abstract.  Author URL.
Smirnoff N (2000). Ascorbic acid: metabolism and functions of a multi-facetted molecule. Curr Opin Plant Biol, 3(3), 229-235. Abstract.  Author URL.
Davey MW, Van Montagu M, Inzé D, Sanmartin M, Kanellis A, Smirnoff N, Benzie IFF, Strain JJ, Favell D, Fletcher J, et al (2000). Plant L-ascorbic acid: Chemistry, function, metabolism, bioavailability and effects of processing. Journal of the Science of Food and Agriculture, 80(7), 825-860. Abstract.
Proctor MC, Smirnoff N (2000). Rapid recovery of photosystems on rewetting desiccation-tolerant mosses: chlorophyll fluorescence and inhibitor experiments. J Exp Bot, 51(351), 1695-1704. Abstract.  Author URL.
Spickett CM, Smirnoff N, Pitt AR (2000). The biosynthesis of erythroascorbate in Saccharomyces cerevisiae and its role as an antioxidant. Free Radic Biol Med, 28(2), 183-192. Abstract.  Author URL.
Pallanca JE, Smirnoff N (2000). The control of ascorbic acid synthesis and turnover in pea seedlings. J Exp Bot, 51(345), 669-674. Abstract.  Author URL.
Pallanca JE, Smirnoff N (1999). Ascorbic acid metabolism in pea seedlings. A comparison of D-glucosone, L-sorbosone, and L-galactono-1,4-lactone as ascorbate precursors. Plant Physiology, 120(2), 453-461. Abstract.
Pallanca JE, Smirnoff N (1999). Ascorbic acid metabolism in pea seedlings. A comparison of D-glucosone, L-sorbosone, and L-galactono-1,4-lactone as ascorbate precursors. Plant Physiol, 120(2), 453-462. Abstract.  Author URL.
Smirnoff N, Bryant JA (1999). DREB takes the stress out of growing up. Nat Biotechnol, 17(3), 229-230.  Author URL.
Dixon KP, Xu JR, Smirnoff N, Talbot NJ (1999). Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by Magnaporthe grisea. Plant Cell, 11(10), 2045-2058. Abstract.  Author URL.
Macnair MR, Smirnoff N (1999). Use of zincon to study uptake and accumulation of zinc by zinc tolerant and hyperaccumulating plants. Communications in Soil Science and Plant Analysis, 30(7-8), 1127-1136. Abstract.
Marschall M, Proctor MCF, Smirnoff N (1998). Carbohydrate composition and invertase activity of the leafy liverwort Porella platyphylla. New Phytologist, 138(2), 343-353. Abstract.
Barnwell P, Blanchard AN, Bryant JA, Smirnoff N, Weir AF (1998). Isolation of DNA from the Highly Mucilagenous Succulent Plant Sedum telephium. Plant Molecular Biology Reporter, 16(2). Abstract.
Smirnoff N (1998). Plant resistance to environmental stress. Curr Opin Biotechnol, 9(2), 214-219. Abstract.  Author URL.
Wheeler GL, Jones MA, Smirnoff N (1998). The biosynthetic pathway of vitamin C in higher plants. Nature, 393(6683), 365-369. Abstract.  Author URL.
Csintalan Z, Takacs Z, Tuba Z, Proctor MCF, Smirnoff N, Grace J (1997). Desiccation tolerant grassland cryptogams under elevated CO<inf>2</inf>: Preliminary findings. Abstracta Botanica, 21(2), 309-315. Abstract.
De Jong JC, McCormack BJ, Smirnoff N, Talbot NJ (1997). Glycerol generates turgor in rice blast [5]. Nature, 389(6648), 244-245.
Conklin PL, Pallanca JE, Last RL, Smirnoff N (1997). L-ascorbic acid metabolism in the ascorbate-deficient arabidopsis mutant vtc1. Plant Physiol, 115(3), 1277-1285. Abstract.  Author URL.
Tuba Z, Smirnoff N, Csintalan Z, Szente K, Nagy Z (1997). Respiration during slow desiccation of the poikilochlorophyllous desiccation tolerant plant Xerophyta scabrida at present-day CO<inf>2</inf> concentration. Plant Physiology and Biochemistry, 35(5), 381-386. Abstract.
Smirnoff N (1996). The function and metabolism of ascorbic acid in plants. Annals of Botany, 78(6), 661-669. Abstract.
Webster J, Davey RA, Smirnoff N, Fricke W, Hinde P, Tomos D, Turner JCR (1995). Mannitol and hexoses are components of Buller's drop. Mycological Research, 99(7), 833-838. Abstract.
OVERY S, SMIRNOFF N (1995). PROLINE DEHYDROGENASE AND PROLINE ACCUMULATION IN PISUM-SATIVUM AT LOW WATER POTENTIAL. PLANT PHYSIOLOGY, 108(2), 66-66.  Author URL.
CONTI S, SMIRNOFF N (1994). RAPID TRIGGERING OF MALATE ACCUMULATION IN THE C-3/CAM INTERMEDIATE PLANT SEDUM-TELEPHIUM - RELATIONSHIP WITH WATER STATUS AND PHOSPHOENOLPYRUVATE CARBOXYLASE. JOURNAL OF EXPERIMENTAL BOTANY, 45(280), 1613-1621.  Author URL.
Conti S, Smirnoff N (1994). Rapid triggering of malate accumulation in the C<inf>3</inf>/CAM intermediate plant Sedum telephium: Relationship with water status and phosphoenolpyruvate carboxylase. Journal of Experimental Botany, 45(11), 1613-1621. Abstract.
Spickett CM, Smirnoff N, Ratcliffe RG (1993). An in Vivo Nuclear Magnetic Resonance Investigation of Ion Transport in Maize (Zea mays) and Spartina anglica Roots during Exposure to High Salt Concentrations. Plant Physiol, 102(2), 629-638. Abstract.  Author URL.
Conti S, Smirnoff N (1993). Perception of soil water deficit and cam induction in sedu telephium:The role of roots and growth regulators. Giornale Botanico Italiano, 127(5), 943-944.
SMIRNOFF N (1993). TANSLEY REVIEW. 52. THE ROLE OF ACTIVE OXYGEN IN THE RESPONSE OF PLANTS TO WATER-DEFICIT AND DESICCATION. NEW PHYTOLOGIST, 125(1), 27-58.  Author URL.
Spickett CM, Smirnoff N, Ratcliffe RG (1992). Metabolic Response of Maize Roots to Hyperosmotic Shock : an in VivoP Nuclear Magnetic Resonance Study. Plant Physiol, 99(3), 856-863. Abstract.  Author URL.
Stewart GR, Joly CA, Smirnoff N (1992). Partitioning of inorganic nitrogen assimilation between the roots and shoots of cerrado and forest trees of contrasting plant communities of South East Brasil. Oecologia, 91(4), 511-517. Abstract.
GROENHOF AC, SMIRNOFF N, BRYANT JA (1990). THE APPEARANCE OF a NEW MOLECULAR-SPECIES OF PHOSPHOENOLPYRUVATE CARBOXYLASE (PEPC) AND THE RAPID INDUCTION OF CAM IN SEDUM-TELEPHIUM L. PLANT CELL AND ENVIRONMENT, 13(5), 437-445.  Author URL.
Smirnoff N, Cumbes QJ (1989). Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry, 28(4), 1057-1060. Abstract.
SMIRNOFF N, COLOMBE SV (1988). DROUGHT INFLUENCES THE ACTIVITY OF ENZYMES OF THE CHLOROPLAST HYDROGEN-PEROXIDE SCAVENGING SYSTEM. JOURNAL OF EXPERIMENTAL BOTANY, 39(205), 1097-1108.  Author URL.
Smirnoff N, Colombé SV (1988). Drought influences the activity of enzymes of the chloroplast hydrogen peroxide scavenging system. Journal of Experimental Botany, 39(8), 1097-1108. Abstract.
GROENHOF AC, SMIRNOFF N, BRYANT JA (1988). ENZYMIC ACTIVITIES ASSOCIATED WITH THE ABILITY OF AERIAL AND SUBMERGED FORMS OF LITTORELLA-UNIFLORA (L) ASCHERS TO PERFORM CAM. JOURNAL OF EXPERIMENTAL BOTANY, 39(200), 353-361.  Author URL.
SMIRNOFF N, STEWART GR (1987). GLUTAMINE SYNTHETASE AND AMMONIUM ASSIMILATION IN ROOTS OF ZINC‐TOLERANT AND NON‐TOLERANT CLONES OF DESCHAMPSIA CESPITOSA (L.) BEAUV. AND ANTHOXANTHUM ODORATUM L. New Phytologist, 107(4), 659-670. Abstract.
SMIRNOFF N, STEWART GR (1987). NITROGEN ASSIMILATION AND ZINC TOXICITY TO ZINC‐TOLERANT AND NON‐TOLERANT CLONES OF DESCHAMPSIA CESPITOSA (L.) BEAUV. New Phytologist, 107(4), 671-680. Abstract.
SHAH N, SMIRNOFF N, STEWART GR (1987). PHOTOSYNTHESIS AND STOMATAL CHARACTERISTICS OF STRIGA-HERMONTHICA IN RELATION TO ITS PARASITIC HABIT. PHYSIOLOGIA PLANTARUM, 69(4), 699-703.  Author URL.
INGROUILLE MJ, SMIRNOFF N (1986). THLASPI CAERULESCENS J. &amp; C. PRESL. (T. ALPESTRE L.) IN BRITAIN. New Phytologist, 102(1), 219-233. Abstract.
SMIRNOFF N, STEWART GR (1985). NITRATE ASSIMILATION AND TRANSLOCATION BY HIGHER-PLANTS - COMPARATIVE PHYSIOLOGY AND ECOLOGICAL CONSEQUENCES. PHYSIOLOGIA PLANTARUM, 64(2), 133-140.  Author URL.
SMIRNOFF N, WINSLOW MD, STEWART GR (1985). NITRATE REDUCTASE-ACTIVITY IN LEAVES OF BARLEY (HORDEUM-VULGARE) AND DURUM-WHEAT (TRITICUM-DURUM) DURING FIELD AND RAPIDLY APPLIED WATER DEFICITS. JOURNAL OF EXPERIMENTAL BOTANY, 36(169), 1200-1208.  Author URL.
Smirnoff N, Winslow MD, Stewart GR (1985). Nitrate reductase acivity in leaves of barley (Hordeum vulgare) and durum wheat (Triticum durum) during field and rapidly applied water deficits. Journal of Experimental Botany, 36(8), 1200-1208. Abstract.
Smirnoff N, Stewart GR (1985). Stress metabolites and their role in coastal plants. Vegetatio, 62(1-3), 273-278. Abstract.
Smirnoff N, Todd P, Stewart GR (1984). The occurrence of nitrate reduction in the leaves of woody plants. Annals of Botany, 54(3), 363-374. Abstract.
Smirnoff N, Crawford RMM (1983). Variation in the structure and response to flooding of root aerenchyma in some wetland plants. Annals of Botany, 51(2), 237-249. Abstract.
BROWN DH, SMIRNOFF N (1978). OBSERVATIONS ON EFFECT OF OZONE ON CLADONIA-RANGIFORMIS. LICHENOLOGIST, 10, 91-94.  Author URL.

Chapters

Ishikawa T, Maruta T, Yoshimura K, Smirnoff N (2018). Biosynthesis and regulation of ascorbic acid in plants. In  (Ed) Antioxidants and Antioxidant Enzymes in Higher Plants, 163-179. Abstract.
Smirnoff N (2011). Chapter 4 Vitamin C the Metabolism and Functions of Ascorbic Acid in Plants. In  (Ed) Biosynthesis of Vitamins in Plants Part B, Elsevier, 107-177.

Conferences

Smirnoff N (2022). SHINING a LIGHT ON ASCORBATE METABOLISM.  Author URL.
Breen S, Brown H, Zabala MDT, Arnaud D, Hussain RF, Kulasekaran S, Smirnoff N, Schwarzlaender M, Littlejohn G, Grant MR, et al (2019). Chloroplasts, a major hub of immune signaling.  Author URL.
Gaikwad T, Breen S, Kulasekaran S, Arnaud D, Hussain RF, Smirnoff N, Breeze E, Alfano JR, Littlejohn G, Grant MR, et al (2019). The role of chloroplasts in disease and defence.  Author URL.
Wang C-C, Chandrappa D, Smirnoff N, Moger J (2015). Monitoring Lipid Accumulation in the Green Microalga <i>Botryococcus braunii</i> with Frequency-Modulated Stimulated Raman Scattering.  Author URL.
Smirnoff N (2015). The evolution of vitamin C biosynthetic pathways in plants and algae.  Author URL.
Ishikawa T, Tanimoto M, Dowdle J, Smirnoff N (2007). <i>VTC2</i> encodes GDP-L-galactose phosphorylase, an enzyme involved in plant ascorbate biosynthesis.  Author URL.
Takács Z, Tuba Z, Smirnoff N (2001). Exaggeration of desiccation stress by heavy metal pollution in Tortula ruralis: a pilot study. Abstract.
Smirnoff N (2000). Ascorbate biosynthesis and function in photoprotection. Abstract.  Author URL.
Conklin PL, Norris SR, Wheeler GL, Williams EH, Smirnoff N, Last RL (1999). Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. Abstract.  Author URL.
Smirnoff N, Pallanca JE (1996). Ascorbate metabolism in relation to oxidative stress.  Author URL.
Smirnoff N (1996). Regulation of crassulacean acid metabolism by water status in the C-3/CAM intermediate Sedum telephium.  Author URL.
SMIRNOFF N (1995). ANTIOXIDANT SYSTEMS AND PLANT RESPONSE TO THE ENVIRONMENT.  Author URL.
SMIRNOFF N (1995). METABOLIC FLEXIBILITY IN RELATION TO THE ENVIRONMENT.  Author URL.
POPP M, SMIRNOFF N (1995). POLYOL ACCUMULATION AND METABOLISM DURING WATER DEFICIT.  Author URL.
SAMARAS Y, BRESSAN RA, CSONKA LN, GARCIARIOS MG, DURZO MP, RHODES D (1995). PROLINE ACCUMULATION DURING DROUGHT AND SALINITY.  Author URL.
NIMMO HG, CARTER PJ, FEWSON CA, NELSON JPS, NIMMO GA, WILKINS MB (1995). REGULATION OF MALATE SYNTHESIS IN CAM PLANTS AND GUARD CELLS - EFFECTS OF LIGHT AND TEMPERATURE ON THE PHOSPHORYLATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE.  Author URL.
ORTHEN B, POPP M, SMIRNOFF N (1994). HYDROXYL RADICAL SCAVENGING PROPERTIES OF CYCLITOLS.  Author URL.
Smirnoff N (1992). The carbohydrates of bryophytes in relation to desiccation tolerance. Abstract.
SMIRNOFF N, RATCLIFFE RG (1990). EARLY RESPONSES OF PEA ROOTS TO WATER DEFICIT STUDIED WITH P-31-NMR.  Author URL.
GROENHOF AC, SMIRNOFF N, BRYANT JA (1990). REGULATION OF THE C3 TO CAM SWITCH IN SEDUM-TELEPHIUM BY WATER-STRESS.  Author URL.
SLEE NJD, BRYANT JA, SMIRNOFF N, SMITH BG (1990). THE EFFECT OF WATER DEFICIT AND ABA ON PROTEIN-SYNTHESIS IN SUNFLOWERS (HELIANTHUS-ANNUUS).  Author URL.
HUGHES SG, BRYANT JA, SMIRNOFF N (1989). MOLECULAR-BIOLOGY - APPLICATION TO STUDIES OF STRESS TOLERANCE.  Author URL.
Smirnoff N, Stewart GR, Todd P (1983). THE OCCURRENCE OF NITRATE REDUCTION IN THE LEAVES OF WOODY PLANTS.  Author URL.

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External Engagement and Impact

Committee/panel activities

GARNet (BBSRC-funded Genomic Resources for Arabidopsis Committee)- Elected member of advisory board (2009-2012)

BBSRC Agri-Food Committee (2004).

BBSRC Committee B core member (2013-)

Grant award panel for Finnish Academy of Sciences plant ecophysiology programme (2005).

Society for Experimental Biology (SEB): Plant Biology Committee (1992-1995 and 1999-present).

Vice-Chair of Plant Biology Committee (2001).

Council of SEB (2001-2006).

Chair of SEB Plant Metabolism Group (1999-2006).


Editorial responsibilities

Editor "Plant Physiology" (2018-)

Editor of 'Annals of Botany' (1998-present).

Journal of Experimental Botany Handling Editor 2012-present

Regular reviewer for many journals including Nature, Nature Biotechnology, Plant Journal, Plant Physiology and Plant Cell.

Member of the editorial advisory board of "Journal of Experimental Botany" (1994-2012).


Invited lectures

Phytochemical Society of America (Hawaii, Dec 2011)

11th International POG Conference- Reactive Oxygen and Nitrogen Speciers in Plants (Warsaw, 2013)

Seminar (University of Leeds Feb 2013)

Plant Synthetic Biology (GARNet, Nottingham May 2013)

Keystone conference on nutritional enhancement of food. Boulder, Colorado. April 2001.

Seminis Vegetable Seeds (Woodland, California) April 2002.

Gordon Research Conference on Postharvest Physiology, Boston, August 2002

Second International Conference of Plant Metabolomics. Potsdam, April 2003.

Gordon Research Conference on senescence programmed cell death, Boston, July 2004.

Royal Society of Chemistry, Sept 2004. Discussion meeting on vitamin C.

*Rothamsted Research (January 2005). Launch meeting for Phase 2 of the BBSRC Plant and Microbial Metabolomics Initiative.

Plant Oxygen Group (Dec 2005). Bristol.

*Royal Society of Chemistry. September 2007. Bioinorganic group meeting on biosynthetic pathways (Firbush, Loch Tay).

Scottish Crops Research Institute, Invergowrie (April 2002).

Rothamsted Research

University of Nottingham (April 2002

University of Cambridge (February 2003).

University of Essex (February 2005)

University of Birmingham (January 2006)


Media Coverage

BBC2- "How to Grow a Planet" (Feb 2012)- contributed an experiment which was broadcast as part of the first installment ("Life from Light") of this 3 part series.  

BBC Radio 4 "Growing Science". (4 Nov 2002) presented by Pippa Greenwood.; http://www.bbc.co.uk/radio4/science/growingscience.shtml)

BBC Radio 4 "The Eureka Years" presented by Adam Hart-Davies (Sept 2004)


Workshops/Conferences organised

Advisory Board. 11th International POG Conference- Reactive Oxygen and Nitrogen Speciers in Plants (Warsaw, 2013)

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Teaching

Modules

2023/24

Information not currently available


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Supervision / Group

Postdoctoral researchers

  • David Cuitun-Coronado Postdoctoral Research Fellow: ascorbic acid metabolism and function (FEDORA Project)
  • Chloe Singleton

Postgraduate researchers

  • Isobel Cole The occurrence and Function of ergothioneine and ovothiol in photosynthetic organisms

Alumni

  • Dominique Arnaud Postdoctoral Research Fellow- the role of hydrgen peroxide in stomatal guard cells
  • Felicia Charles-Johnson
  • Louise Colville
  • Marino Exposito-Rodriguez
  • Thaura Ghneim
  • Choon Kiat Lim
  • Anja Nenninger
  • Sarah Overy
  • Deborah Salmon Mass Spectrometry Experimental Officer
  • Nicola Slee
  • Susanna Smith
  • Kevin Spelman
  • Nighat Sultana
  • George Taylor
  • Angela Thymides
  • Bridget Trezise
  • Glen Wheeler

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