Dr Hsueh-Lui Ho
Senior Experimental Officer
2010 PhD Clinical Medicine - Fungal Biology, Imperial College London
2004 MSc Biochemistry, University of Kent at Canterbury
2003 BSc Biochemistry, Imperial College London
April 2011 – Present: Senior Experimental Officer, Exeter University
2010 – 2011: Postdoctoral researcher, Imperial College London
2008-2010: Research associate, Imperial College London
1: Understanding the spatial and temporal nature of the cell separation machinery of Saccharomyces cerevisiae
The final act of the cell cycle in Saccharomyces cerevisiae is cell separation. Inactivation of the transcription factor encoding gene, ACE2, leads to a defect in cell separation. Ace2 plays a central role in cell separation by regulating daughter cell specific expression of endochitinase (CTS1) and at least 3 putative glucanase encoding genes, DSE2, DSE4 (ENG1) and SCW11. The products of these genes degrade the tri-laminar septum that holds mother and daughter cells together. ACE2, itself, is regulated by the RAM (Regulation of Ace2 activity and cellular Morphogenesis) network; inactivation of RAM network proteins results in defects in cell separation and mis-localisation of Ace2.
In order to define the components of the cell separation machinery in S. cerevisiae, the non-essential genes in the yeast knockout (YKO) library and essential genes in the yeast Tet-promoters Hughes Collection (yTHC) were screened for mutants that failed to separate. Novel mutants not previously linked to cell separation are then selected for further characterisation.
2: Construction of a Candida glabrata ORFeome
Candida glabrata is an opportunistic pathogen that is predominantly found in the elderly and immune-compromised individuals and is of increasing medical concern due to the increased life expectancy of the population and its lack of susceptibility to some current anti-fungal treatments. Little is known about its virulence mechanisms, and unlike Saccharomyces cerevisiae, few community tools are available to explore this pathogen.
Using the GATEWAY system, we will construct a C. glabrata ORFeome and on its completion it will be released to the community.
3: Protein interaction network variation between Saccharomyces cerevisiae and Candida glabrata
Phylogentically Candida glabrata is most closely related to the non-pathogen Saccharomyces cerevisiae ; approximately 77% of C. glabrata proteins have orthologues in S. cerevisiae. Thus, a common assumption made between the two species is that orthologues share a similar function and protein-protein interaction (PPI) profile.
Using the GATEWAY system, we will examine the common assumption that orthologues share a similar function and PPI profile.
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Hsueh_Li_Ho Details from cache as at 2018-07-17 15:55:40