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Dr Paul James

Dr Paul James

Industrial Research Fellow

 3105

 01392723105

 Biocatalysis Centre BioEconomy Office

 

Biocatalysis Centre, University of Exeter, The Henry Wellcome Building for Biocatalysis, Stocker Road, Exeter, EX4 4QD , UK

I am currently a Research Fellow under the supervision of Professor John Love based in the Exeter Microbial Biofuel Group on the Mezzanine floor of the Geoffrey Pope building in the College of Life and Envrironmental Sciences. The group is currently using a synthetic biology approach to producing customisable petroleum replica fuel molecules. 

My previous research interests focused on the utilisation of enzymes from thermophilic organisms and their application in the field of “white biotechnology”. In particular I am interested in expression of various enzymes of interest to the chemical and pharmaceutical industry within E. coli in high levels to perform structural and biochemical characterisation. In particular I am interested in the increased stability of these enzymes to a wide range of conditions such as temperature and the different solvents used in industrial processes. I also use the structure of these enzymes to help me understand the mechanisms employed by different organisms to increase stability.

Qualifications

2011 PhD Biological Sciences
2010 MSc Biological Chemistry
2009 BSc Biological and Medicinal Chemistry

Career

2018 - Industrial Research Fellow, University of Exeter, The BioEconomy Centre 

2014 - Research Fellow, University of Exeter, Exeter Microbial Biofuels Group

2013 - 2014  Associate Research Fellow, University of Exeter, ERA Net IB - Thermogene

2011 - 2013 Associate Research Fellow, University of Exeter, StatOil

Further details of projects can be found under Research

Links

Research

Research projects

Exeter Microbial Biofuels Group (Jan 2014 - Present)

Using synthetic biology for the prodcution of petroleum replica fuel molecules.

The ERA-IB Thermogene Project (Feb 2013 - Jan 2014)


The THERMOGENE project will identify, biochemically and structurally characterize novel thermostable transferase enzymes, from newly sequenced thermostable genomes and metagenomes, which have potential applications for industrial biocatalysis. Four main groups of transferase enzymes will be targeted; the transketolase enzymes which can transfer a two carbon group, the transaminases that transfer an amine group, the soluble prenyl transferases that transfer a prenyl group and the methyl and hydroxymethyl transferases.

StatOil (Nov 2011 - Jan 2013)


Carbonic anhydrases (CAs, E.C. 4.2.1.1) are a family of enzymes that catalyse the reversible conversion of carbon dioxide and water to bicarbonate and a proton. This interconversion has generated a large amount of interest in recent years due to its potential use in the capture and storage of the harmful greenhouse gas CO2. Current CO2 removal systems are in use by industrial companies, mainly in fossil fuel powered plants but the current technology usually involves the use of harmful amine based solvents and corrosive hot potassium carbonates. CA enzymes have been shown to assist the use of the solvents in the chemical process and also as a alternative to the current solvent methods.

PhD - Investigation into Peroxiredoxin and interactions in the Peroxiredoxin peroxide scavenging system
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.547032

Key publications | Publications by category | Publications by year

Publications by category


Journal articles

Mora-Pale M, Bhan N, Masuko S, James P, Wood J, McCallum S, Linhardt RJ, Dordick JS, Koffas MAG (2015). Antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. Biotechnol Bioeng, 112(12), 2417-2428. Abstract.  Author URL.
James P, Isupov MN, Sayer C, Saneei V, Berg S, Lioliou M, Kotlar HK, Littlechild JA (2014). The structure of a tetrameric α-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability. Acta Crystallogr D Biol Crystallogr, 70(Pt 10), 2607-2618. Abstract.  Author URL.  Full text.

Chapters

James PBC, sayer C, Novak H, Littlechild JA (2013). Mechanisms of Thermal Stability Adopted by Thermophilic Proteins and Their Use in White Biotechnology. In Littlechild JA, Satyanarayana T, Kawarabayasi Y (Eds.) Thermophilic Microbes in Environmental and Industrial Biotechnology, Springer Netherlands, 481-507.

Publications by year


2015

Mora-Pale M, Bhan N, Masuko S, James P, Wood J, McCallum S, Linhardt RJ, Dordick JS, Koffas MAG (2015). Antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. Biotechnol Bioeng, 112(12), 2417-2428. Abstract.  Author URL.

2014

James P, Isupov MN, Sayer C, Saneei V, Berg S, Lioliou M, Kotlar HK, Littlechild JA (2014). The structure of a tetrameric α-carbonic anhydrase from Thermovibrio ammonificans reveals a core formed around intermolecular disulfides that contribute to its thermostability. Acta Crystallogr D Biol Crystallogr, 70(Pt 10), 2607-2618. Abstract.  Author URL.  Full text.

2013

James PBC, sayer C, Novak H, Littlechild JA (2013). Mechanisms of Thermal Stability Adopted by Thermophilic Proteins and Their Use in White Biotechnology. In Littlechild JA, Satyanarayana T, Kawarabayasi Y (Eds.) Thermophilic Microbes in Environmental and Industrial Biotechnology, Springer Netherlands, 481-507.

Paul_B_James Details from cache as at 2018-10-15 11:33:56

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I am currently an Instructor to the Exeter University iGEM team. 

Postdoctoral researchers

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