People

All Exeter Staff | All Cornwall Staff

Loading content
Dr Vicki Gold

Dr Vicki Gold

Senior Lecturer

 7454

 +44 (0)1392 727454

 Living Systems Institute T04.16

 

Living Systems Institute, University of Exeter, Stocker Road, Exeter, EX4 4QD

Overview

I am primarily a biochemist whose interest in electron microscopy was sparked by the amazing ability to directly observe individual proteins within whole cells. I now employ different techniques in electron cryo-microscopy to study the fundamental process of protein transport across cell membranes. My lab is interested in two main research themes- mitochondrial protein import and the bacterial type IV pilus assembly machinery. Both of these are important topics which can help us to understand the principles of protein transport, and how mechanisms may be compromised in disease states or exploited on bacterial and viral infection.

I am based in the Living Systems Institute.

Qualifications

2008: PhD Biochemistry, University of Bristol
2004: BSc Biological Sciences, University of Warwick

Career

2016-present: Senior Lecturer, Living Systems Institute, University of Exeter, UK
2011-2016: Postdoctoral Fellow, Department of Structural Biology, Max Planck Institute of Biophysics, Germany
2008-2011: Postdoctoral Research Associate, School of Biochemistry, University of Bristol, UK    
2004-2007: Postgraduate Research Assistant, School of Biochemistry, University of Bristol, UK     

Fig. 1 To locate the mitochondrial import machinery, a pre-protein can be arrested across both mitochondrial membranes and labelled with a dense quantum-dot tag (black spheres). This reveals import site distribution on the outer membrane (green) and with respect to the cristae (yellow) and crista junctions (red box; yellow arrowheads). Blue box; a close up of the import machinery in action with protein densities marked with blue and green arrowheads and the dense label in black. OM, outer membrane; IM, inner membrane.

Fig. 2.Slices through tomograms and corresponding structures determined by sub-tomogram averaging highlight the type IV pilus machinery (boxed) in the closed (blue, pilus retracted) and open (green, pilus assembled) states.

Research

Research interests

The objective of my research is to provide insights into protein transport across membranes using the technique of electron cryo-microscopy. I focus on the themes of mitochondrial protein import and the bacterial type IV pilus assembly machinery, both important fields of study in our quest to understand human health.

Why electron microscopy?

In 2013, modern electron cryo-microscopy achieved a significant resolution breakthrough through development of the direct electron detector camera. By collection of a series of tilted images in the microscope using the technique of electron cryo-tomography, unprecedented details can now be observed of cellular structures and protein machineries in situ. By single-particle electron cryo-microscopy, structures can now be determined of purified proteins and complexes down to atomic level detail. By harnessing this state-of-the-art technology, my lab endeavours to combine different techniques in electron microscopy with other biophysical methods in order to shed light on fundamental biological processes.

 

Mitochondrial protein import

Mitochondria are the primary cellular source of ATP and form an important bioenergetic and metabolic signaling hub. Due to genomic evolution, only 1% of mitochondrial proteins are encoded on mitochondrial DNA; the remainder are imported from the cytosol. Protein translocases are therefore essential for correct protein targeting and localisation, and a growing number of human pathologies have been linked to mitochondria and the import apparatus. We work to understand how protein import into mitochondria works by electron cryo-tomography, single-particle analysis and protein biochemistry. For further detail see Gold, V. et al, Nat Commun (2014), Gold, V. et al, EMBO Rep (2017)Gold, V. et al, Methods Mol Biol (2017).

The bacterial type IV pilus assembly machinery

The bacterial type IV pilus is a several micron long filament that powers cell motility and adhesion. Pili thus enable bacteria to move within and interact with our environment, and are key determinants of virulence and cause of biofilm formation. Certain type IV pilus assembly machineries have a dual-function- they are also capable of DNA uptake, which enables rapid genetic adaptation and the development of resistance to antimicrobials. By study of this complex machine by fluorescence microscopy, electron cryo-tomography, sub-tomogram averaging and single-particle analysis, we aim to alter the way in which bacterial motility and genetic adaptation are viewed. For further detail see Gold, V. et al, eLife (2015) & Gold, V. & Kudrayashev, M., Curr Opin Struct Biol (2016).

Equipment and facilities

We are currently equipped with brand new state-of-the-art laboratories for cryoEM sample preparation, imaging and data processing. We employ a cryo- and automation-capable 120kV microscope (FEI Tecnai Spirit) with CMOS camera for screening samples and generation of initial single particle EM and cryoET data sets. For higher resolution data collection we use our shared GW4 200kV cryo-microscope (FEI Talos Arctica) fitted with a K2 direct electron detector. We are also well equipped with standard wet laboratory equipment for molecular biology, biochemistry, protein expression and purification. 

 

Research networks

  • Prof. Agnieszka Chacinska, IIMCB Warsaw, Poland
  • Prof. Ian Collinson, University of Bristol, UK
  • Prof. Beate Averhoff, University of Frankfurt, Germany
  • GW4 South West Regional Facility for CryoEM

Research grants

  • 2018 BBSRC
    The bacterial type IV pilus machinery as a DNA translocator
  • 2018 Wellcome Trust
    The filamentous phage assembly system as a therapeutic tool
  • 2017 Wellcome Trust
    Electron Cryo-microscopy equipment grant

Publications

Key publications | Publications by category | Publications by year

Key publications


Gold VA, Chroscicki P, Bragoszewski P, Chacinska A (In Press). Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. EMBO Rep, 18(10), 1786-1800. Abstract.  Author URL.  Full text.
Gold VAM, Brandt T, Cavellini L, Cohen MM, Ieva R, van der Laan M (2017). Analysis of mitochondrial membrane protein complexes by electron cryo-tomography. In  (Ed) Methods in Molecular Biology, 315-336.  Abstract.
Gold VAM, Salzer R, Averhoff B, Kühlbrandt W (2015). Structure of a type IV pilus machinery in the open and closed state. Elife, 4 Abstract.  Author URL.  Full text.
Gold VAM, Ieva R, Walter A, Pfanner N, van der Laan M, Kühlbrandt W (2014). Visualizing active membrane protein complexes by electron cryotomography. Nat Commun, 5 Abstract.  Author URL.  Full text.
Gold VAM, Whitehouse S, Robson A, Collinson I (2013). The dynamic action of SecA during the initiation of protein translocation. BIOCHEMICAL JOURNAL, 449, 695-705. Author URL.
Gold VAM, Robson A, Bao H, Romantsov T, Duong F, Collinson I (2010). The action of cardiolipin on the bacterial translocon. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(22), 10044-10049. Author URL.

Publications by category


Journal articles

Gold V, Kudryashev M (In Press). Recent progress in structure and dynamics of dual-membrane-spanning bacterial nanomachines. Curr Opin Struct Biol, 39, 1-7. Abstract.  Author URL.
Gold VA, Chroscicki P, Bragoszewski P, Chacinska A (In Press). Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. EMBO Rep, 18(10), 1786-1800. Abstract.  Author URL.  Full text.
Wang S, Powers R, Gold VAM, Rapoport T (2018). The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discs. Life Science Alliance Full text.
Daum B, Gold V (2018). Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint. Biol Chem, 399(7), 799-808. Abstract.  Author URL.  Full text.
Turakhiya U, von der Malsburg K, Gold VAM, Guiard B, Chacinska A, van der Laan M, Ieva R (2016). Protein Import by the Mitochondrial Presequence Translocase in the Absence of a Membrane Potential. J Mol Biol, 428(6), 1041-1052. Abstract.  Author URL.
Salzer R, D'Imprima E, Gold VAM, Rose I, Drechsler M, Vonck J, Averhoff B (2016). Topology and Structure/Function Correlation of Ring- and Gate-forming Domains in the Dynamic Secretin Complex of Thermus thermophilus. J Biol Chem, 291(28), 14448-14456. Abstract.  Author URL.  Full text.
Gold VAM, Salzer R, Averhoff B, Kühlbrandt W (2015). Structure of a type IV pilus machinery in the open and closed state. Elife, 4 Abstract.  Author URL.  Full text.
Stockburger C, Gold VAM, Pallas T, Kolesova N, Miano D, Leuner K, Müller WE (2014). A cell model for the initial phase of sporadic Alzheimer's disease. J Alzheimers Dis, 42(2), 395-411. Abstract.  Author URL.
Schulze RJ, Komar J, Botte M, Allen WJ, Whitehouse S, Gold VAM, Nijeholtb JALA, Huard K, Berger I, Schaffitzel C, et al (2014). Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(13), 4844-4849. Author URL.
Davies KM, Daum B, Gold VAM, Mühleip AW, Brandt T, Blum TB, Mills DJ, Kühlbrandt W (2014). Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography. J Vis Exp(91). Abstract.  Author URL.  Full text.
Gold VAM, Ieva R, Walter A, Pfanner N, van der Laan M, Kühlbrandt W (2014). Visualizing active membrane protein complexes by electron cryotomography. Nat Commun, 5 Abstract.  Author URL.  Full text.
Gold VAM, Whitehouse S, Robson A, Collinson I (2013). The dynamic action of SecA during the initiation of protein translocation. BIOCHEMICAL JOURNAL, 449, 695-705. Author URL.
Whitehouse S, Gold VAM, Robson A, Allen WJ, Sessions RB, Collinson I (2012). Mobility of the SecA 2-helix-finger is not essential for polypeptide translocation via the SecYEG complex. JOURNAL OF CELL BIOLOGY, 199(6), 919-929. Author URL.
Hizlan D, Robson A, Whitehouse S, Gold VA, Vonck J, Mills D, Kühlbrandt W, Collinson I (2012). Structure of the SecY complex unlocked by a preprotein mimic. Cell Rep, 1(1), 21-28. Abstract.  Author URL.
Deville K, Gold VAM, Robson A, Whitehouse S, Sessions RB, Baldwin SA, Radford SE, Collinson I (2011). The Oligomeric State and Arrangement of the Active Bacterial Translocon. JOURNAL OF BIOLOGICAL CHEMISTRY, 286(6), 4659-4669. Author URL.
Gold VAM, Robson A, Bao H, Romantsov T, Duong F, Collinson I (2010). The action of cardiolipin on the bacterial translocon. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(22), 10044-10049. Author URL.
Robson A, Gold VAM, Hodson S, Clarke AR, Collinson I (2009). Energy transduction in protein transport and the ATP hydrolytic cycle of SecA. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(13), 5111-5116. Author URL.
Robson A, Booth AEG, Gold VAM, Clarke AR, Collinson I (2007). A large conformational change couples the ATP binding site of SecA to the SecY protein channel. JOURNAL OF MOLECULAR BIOLOGY, 374(4), 965-976. Author URL.
Gold VAM, Robson A, Clarke AR, Collinson I (2007). Allosteric regulation of SecA - Magnesium-mediated control of conformation and activity. JOURNAL OF BIOLOGICAL CHEMISTRY, 282(24), 17424-17432. Author URL.
Gold VAM, Duong F, Collinson I (2007). Structure and function of the bacterial Sec translocon. MOLECULAR MEMBRANE BIOLOGY, 24(5-6), 387-394. Author URL.

Chapters

Gold VAM, Brandt T, Cavellini L, Cohen MM, Ieva R, van der Laan M (2017). Analysis of mitochondrial membrane protein complexes by electron cryo-tomography. In  (Ed) Methods in Molecular Biology, 315-336.  Abstract.

Publications by year


In Press

Gold V, Kudryashev M (In Press). Recent progress in structure and dynamics of dual-membrane-spanning bacterial nanomachines. Curr Opin Struct Biol, 39, 1-7. Abstract.  Author URL.
Gold VA, Chroscicki P, Bragoszewski P, Chacinska A (In Press). Visualization of cytosolic ribosomes on the surface of mitochondria by electron cryo-tomography. EMBO Rep, 18(10), 1786-1800. Abstract.  Author URL.  Full text.

2018

Wang S, Powers R, Gold VAM, Rapoport T (2018). The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discs. Life Science Alliance Full text.
Daum B, Gold V (2018). Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint. Biol Chem, 399(7), 799-808. Abstract.  Author URL.  Full text.

2017

Gold VAM, Brandt T, Cavellini L, Cohen MM, Ieva R, van der Laan M (2017). Analysis of mitochondrial membrane protein complexes by electron cryo-tomography. In  (Ed) Methods in Molecular Biology, 315-336.  Abstract.

2016

Turakhiya U, von der Malsburg K, Gold VAM, Guiard B, Chacinska A, van der Laan M, Ieva R (2016). Protein Import by the Mitochondrial Presequence Translocase in the Absence of a Membrane Potential. J Mol Biol, 428(6), 1041-1052. Abstract.  Author URL.
Salzer R, D'Imprima E, Gold VAM, Rose I, Drechsler M, Vonck J, Averhoff B (2016). Topology and Structure/Function Correlation of Ring- and Gate-forming Domains in the Dynamic Secretin Complex of Thermus thermophilus. J Biol Chem, 291(28), 14448-14456. Abstract.  Author URL.  Full text.

2015

Gold VAM, Salzer R, Averhoff B, Kühlbrandt W (2015). Structure of a type IV pilus machinery in the open and closed state. Elife, 4 Abstract.  Author URL.  Full text.

2014

Stockburger C, Gold VAM, Pallas T, Kolesova N, Miano D, Leuner K, Müller WE (2014). A cell model for the initial phase of sporadic Alzheimer's disease. J Alzheimers Dis, 42(2), 395-411. Abstract.  Author URL.
Schulze RJ, Komar J, Botte M, Allen WJ, Whitehouse S, Gold VAM, Nijeholtb JALA, Huard K, Berger I, Schaffitzel C, et al (2014). Membrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(13), 4844-4849. Author URL.
Davies KM, Daum B, Gold VAM, Mühleip AW, Brandt T, Blum TB, Mills DJ, Kühlbrandt W (2014). Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography. J Vis Exp(91). Abstract.  Author URL.  Full text.
Gold VAM, Ieva R, Walter A, Pfanner N, van der Laan M, Kühlbrandt W (2014). Visualizing active membrane protein complexes by electron cryotomography. Nat Commun, 5 Abstract.  Author URL.  Full text.

2013

Gold VAM, Whitehouse S, Robson A, Collinson I (2013). The dynamic action of SecA during the initiation of protein translocation. BIOCHEMICAL JOURNAL, 449, 695-705. Author URL.

2012

Whitehouse S, Gold VAM, Robson A, Allen WJ, Sessions RB, Collinson I (2012). Mobility of the SecA 2-helix-finger is not essential for polypeptide translocation via the SecYEG complex. JOURNAL OF CELL BIOLOGY, 199(6), 919-929. Author URL.
Hizlan D, Robson A, Whitehouse S, Gold VA, Vonck J, Mills D, Kühlbrandt W, Collinson I (2012). Structure of the SecY complex unlocked by a preprotein mimic. Cell Rep, 1(1), 21-28. Abstract.  Author URL.

2011

Deville K, Gold VAM, Robson A, Whitehouse S, Sessions RB, Baldwin SA, Radford SE, Collinson I (2011). The Oligomeric State and Arrangement of the Active Bacterial Translocon. JOURNAL OF BIOLOGICAL CHEMISTRY, 286(6), 4659-4669. Author URL.

2010

Gold VAM, Robson A, Bao H, Romantsov T, Duong F, Collinson I (2010). The action of cardiolipin on the bacterial translocon. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(22), 10044-10049. Author URL.

2009

Robson A, Gold VAM, Hodson S, Clarke AR, Collinson I (2009). Energy transduction in protein transport and the ATP hydrolytic cycle of SecA. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(13), 5111-5116. Author URL.

2007

Robson A, Booth AEG, Gold VAM, Clarke AR, Collinson I (2007). A large conformational change couples the ATP binding site of SecA to the SecY protein channel. JOURNAL OF MOLECULAR BIOLOGY, 374(4), 965-976. Author URL.
Gold VAM, Robson A, Clarke AR, Collinson I (2007). Allosteric regulation of SecA - Magnesium-mediated control of conformation and activity. JOURNAL OF BIOLOGICAL CHEMISTRY, 282(24), 17424-17432. Author URL.
Gold VAM, Duong F, Collinson I (2007). Structure and function of the bacterial Sec translocon. MOLECULAR MEMBRANE BIOLOGY, 24(5-6), 387-394. Author URL.

Vicki_Gold Details from cache as at 2018-12-09 16:27:11

Refresh publications

External Engagment and Impact

Administrative responsibilities

Steering committee for the South West Regional Facility for CryoEM, GW4 and University of Exeter                  

 


Awards

EMBO Long-Term Fellowship, held at the Max-Planck Institute of Biophysics, Frankfurt am Main, Germany (2011-2013)

Lead PI on eBic BAG (Block Allocation Group) for time at the  Electron Bio-Imaging Centre (eBIC) at Diamond

Teaching

Supervision / Group

Postdoctoral researchers

  • Becky Conners
  • Alexander Neuhaus

Research Technicians

  • Mathew McLaren
  • Kelly Sanders

Back | Edit Profile