+44 (0) 1392 725296
Geoffrey Pope 309
Geoffrey Pope Building, University of Exeter , Stocker Road, Exeter, EX4 4QD, UK
One hundred percent - 96/96 Mycosphaerella graminicola ORFs cloned - the first time ever in our BBSRC funded ORFeome construction effort.
2003-2007: B. Pharm University of Pune, India.
2007-2008: MSc Biotechnology, University of Abertay Dundee, United Kingdom.
Aug 2008 - Aug 2009: Manchester Institute of Biotechnology, University of Manchester
Sep 2009 - Apr 2012: Aberdeen Fungal Group, University of Aberdeen
Apr 2012 - present: Ken Haynes Lab, University of Exeter
I am currently working at Ken Haynes lab on Zymoseptoria tritici which is a devastating wheat fungal pathogen. The aim of my project is to construct the fully sequence verified Z. tritici ORFeome library using high-throughput Gateway cloning technology. In this project so far, I have cloned 3,775 Illumina sequence verified ORF’s into E. coli Gateway® entry vector pDONR 207. On completion of this project, collections made will be released to the research community to understand the pathogenicity of this destructive wheat pathogen. I am also performing large scale screening of our Z. tritici over-expression libraries in the heterologous system Saccharomyces cerevisiae.
I am (along with Dr Graham Thomas) also trying to identify the genes involved in Z. tritici spore germination and establishment of disease. We aim to achieve this using RNA-seq approach.
In addition to my research responsibilities at Haynes lab, I also take care of many of the management and administration tasks required to run a successful lab.
Sidhu YS, Chaudhari YK, Usher J, Cairns TC, Csukai M, Haynes K. A suite of Gateway® compatible ternary expression vectors for functional analysis in Zymoseptoria tritici. Fungal Genet Biol. 2015 Jun;79:180-5.
Cairns TC, Sidhu YS, Chaudhari YK, Talbot NJ, Studholme DJ, Haynes K. Construction and high-throughput phenotypic screening of Zymoseptoria tritici over-expression strains. Fungal Genet Biol. 2015 Jun;79:110-7.
Sidhu YS, Cairns TC, Chaudhari YK, Usher J, Talbot NJ, Studholme DJ, Csukai M, Haynes K. Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici. Fungal Genet Biol. 2015 Jun;79:102-9.
Cabral V, Chauvel M, Firon A, Legrand M, Nesseir A, Bachellier-Bassi S, Chaudhari Y, Munro CA, d'Enfert C. Modular gene over-expression strategies for Candida albicans. Methods Mol Biol. 2012;845:227-44.