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Dr Helen Dawe

Dr Helen Dawe

Senior Lecturer in Cell Biology

 3431

 Geoffrey Pope 214

 

Geoffrey Pope Building, University of Exeter , Stocker Road, Exeter, EX4 4QD, UK

cilia sem

I am a molecular cell biologist with interests in cell polarity, cell morphogenesis and the cytoskeleton. My research focuses on how eukaryotic flagella/cilia are built, and how this goes wrong in human inherited disease. I am particularly interested in the early stages of ciliogenesis: centrosome migration and docking at the cell surface, and how these go awry in Meckel-Gruber and Joubert syndromes. I am a member of the Cell Biology research group.

Qualifications

1999-2003 PhD Molecular Cell Biology, MRC Laboratory for Molecular Cell Biology, University of London
1995-1999 BSc (Hons) Biochemistry with Industrial Experience, University of Manchester

Career

2009-present Lecturer in Cell Biology, University of Exeter
2008-2009 University Research Lecturer, University of Oxford
2006-2009 Beit Memorial Fellow, University of Oxford, and Fellow of Brasenose College Oxford
2003-2006 Postdoctoral Research Associate, University of Oxford

Primary cilia IF

MKS2 Composite

Research

Research interests

Eukaryotic cilia and flagella are remarkably conserved, both at the structural and functional levels, from evolutionarily ancient unicellular eukaryotes to man and are adapted to carry out diverse roles. Almost every cell in the human body can form a cilium or flagellum, either motile or non-motile. Motile cilia move fluids past epithelial cell layers in multicellular organisms; however, non-motile primary cilia are the most common type of cilia in the body. The roles of primary cilia were unclear but the recent identification of many inherited disorders involving aberrant ciliary function, termed “ciliopathies”, has changed this and primary cilia are now known as sensory organelles, acting both as chemo- or mechanosensors and transducers of signals that regulate key developmental signalling pathways. My lab focuses on the severe ciliopathy Meckel Gruber syndrome. We use a combination of patient-derived cell lines, post-genomic technologies, classical cell biology and genetics to give insight into the molecular cell biology of the disease. We are currently studying the roles that two proteins, TMEM67 and TMEM216, play in organisation of the actin and microtubule cytoskeletons during cell migration.

Research projects

Current research falls into the following overlapping projects:

  • the mechanism of centrosome migration to the apical membrane during ciliogenesis of primary cilia
  • the molecular cell biology of Meckel-Gruber and Joubert syndromes
  • the role of MKS proteins in non-ciliated cells
  • the role of cilia in setting up the left-right axis in invertebrates

Research networks

Dr Colin Johnson (Leeds Institute for Molecular Medicine)
Dr John Sayer (University of Newcastle)
Prof. Keith Gull (University of Oxford)
Dr Sue Vaughan (Oxford Brookes University)
Dr Lorna Harries (Exeter Medical School)

Research grants

  • 2011 MRC
    New Investigator Award "The Role of Meckel-Gruber Syndrome Proteins in Cell Migration"

Key publications | Publications by category | Publications by year

Key publications


Pratt MB, Titlow JS, Davis I, Barker AR, Dawe HR, Raff JW, Roque H (In Press). Drosophila sensory cilia lacking MKS proteins exhibit striking defects in development but only subtle defects in adults. J Cell Sci, 129(20), 3732-3743. Abstract.  Author URL.
Barker AR, McIntosh KV, Dawe HR (2016). Centrosome positioning in non-dividing cells. Protoplasma, 253(4), 1007-1021. Abstract.  Author URL.
Martin-Urdiroz M, Deeks MJ, Horton CG, Dawe HR, Jourdain I (2016). The Exocyst Complex in Health and Disease. Frontiers in Cell and Developmental Biology, 4 Full text.
Barker AR, Renzaglia KS, Fry K, Dawe HR (2014). Bioinformatic analysis of ciliary transition zone proteins reveals insights into the evolution of ciliopathy networks. BMC Genomics, 15 Abstract.  Author URL.  Full text.
Barker AR, Thomas R, Dawe HR (2014). Meckel-Gruber syndrome and the role of primary cilia in kidney, skeleton, and central nervous system development. Organogenesis, 10(1), 96-107. Abstract.  Author URL.
Adams M, Simms RJ, Abdelhamed Z, Dawe HR, Szymanska K, Logan CV, Wheway G, Pitt E, Gull K, Knowles MA, et al (2012). A meckelin-filamin a interaction mediates ciliogenesis. Human Molecular Genetics, 21(6), 1272-1286. Abstract.

Publications by category


Journal articles

Pratt MB, Titlow JS, Davis I, Barker AR, Dawe HR, Raff JW, Roque H (In Press). Drosophila sensory cilia lacking MKS proteins exhibit striking defects in development but only subtle defects in adults. J Cell Sci, 129(20), 3732-3743. Abstract.  Author URL.
Barker AR, McIntosh KV, Dawe HR (2016). Centrosome positioning in non-dividing cells. Protoplasma, 253(4), 1007-1021. Abstract.  Author URL.
Martin-Urdiroz M, Deeks MJ, Horton CG, Dawe HR, Jourdain I (2016). The Exocyst Complex in Health and Disease. Frontiers in Cell and Developmental Biology, 4 Full text.
Barker AR, Renzaglia KS, Fry K, Dawe HR (2014). Bioinformatic analysis of ciliary transition zone proteins reveals insights into the evolution of ciliopathy networks. BMC Genomics, 15 Abstract.  Author URL.  Full text.
Locke JM, Da Silva Xavier G, Dawe HR, Rutter GA, Harries LW (2014). Increased expression of miR-187 in human islets from individuals with type 2 diabetes is associated with reduced glucose-stimulated insulin secretion. Diabetologia, 57(1), 122-128. Abstract.  Full text.
Barker AR, Thomas R, Dawe HR (2014). Meckel-Gruber syndrome and the role of primary cilia in kidney, skeleton, and central nervous system development. Organogenesis, 10(1), 96-107. Abstract.  Author URL.
Barker AR, McIntosh K, Dawe HR (2013). Meckel-Gruber syndrome proteins are required for actin cytoskeleton organisation and directional cell migration. MOLECULAR BIOLOGY OF THE CELL, 24 Author URL.
Adams M, Simms RJ, Abdelhamed Z, Dawe HR, Szymanska K, Logan CV, Wheway G, Pitt E, Gull K, Knowles MA, et al (2012). A meckelin-filamin a interaction mediates ciliogenesis. Hum Mol Genet, 21(6), 1272-1286. Abstract.  Author URL.
Adams M, Simms RJ, Abdelhamed Z, Dawe HR, Szymanska K, Logan CV, Wheway G, Pitt E, Gull K, Knowles MA, et al (2012). A meckelin-filamin a interaction mediates ciliogenesis. Human Molecular Genetics, 21(6), 1272-1286. Abstract.
Cheng Y-Z, Eley L, Hynes A-M, Overman LM, Simms RJ, Barker A, Dawe HR, Lindsay S, Sayer JA (2012). Investigating embryonic expression patterns and evolution of AHI1 and CEP290 genes, implicated in Joubert syndrome. PLoS One, 7(9). Abstract.  Author URL.
Simms RJ, Hynes AM, Eley L, Inglis D, Chaudhry B, Dawe HR, Sayer JA (2012). Modelling a ciliopathy: Ahi1 knockdown in model systems reveals an essential role in brain, retinal, and renal development. Cellular and Molecular Life Sciences, 69(6), 993-1009. Abstract.
Simms RJ, Hynes AM, Eley L, Inglis D, Chaudhry B, Dawe HR, Sayer JA (2012). Modelling a ciliopathy: Ahi1 knockdown in model systems reveals an essential role in brain, retinal, and renal development. Cell Mol Life Sci, 69(6), 993-1009. Abstract.  Author URL.
Thompson H, Shaw MK, Dawe HR, Shimeld SM (2012). The formation and positioning of cilia in Ciona intestinalis embryos in relation to the generation and evolution of chordate left-right asymmetry. Dev Biol, 364(2), 214-223. Abstract.  Author URL.
Vaughan S, Dawe HR (2011). Common themes in centriole and centrosome movements. Trends Cell Biol, 21(1), 57-66. Abstract.  Author URL.  Full text.
Gluenz E, Höög JL, Smith AE, Dawe HR, Shaw MK, Gull K (2010). Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans. FASEB J, 24(9), 3117-3121. Abstract.  Author URL.
Dawe HR, Adams M, Wheway G, Szymanska K, Logan CV, Noegel AA, Gull K, Johnson CA (2009). Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton. J Cell Sci, 122(Pt 15), 2716-2726. Abstract.  Author URL.  Full text.
Dawe HR, Gluenz E (2009). The tale of the trypanosome tail. Biologist, 56(4), 216-220.
Dawe HR, Farr H, Gull K (2007). Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells. J Cell Sci, 120(Pt 1), 7-15. Abstract.  Author URL.
Dawe HR, Smith UM, Cullinane AR, Gerrelli D, Cox P, Badano JL, Blair-Reid S, Sriram N, Katsanis N, Attie-Bitach T, et al (2007). The Meckel-Gruber Syndrome proteins MKS1 and meckelin interact and are required for primary cilium formation. Hum Mol Genet, 16(2), 173-186. Abstract.  Author URL.
Dawe HR, Shaw MK, Farr H, Gull K (2007). The hydrocephalus inducing gene product, Hydin, positions axonemal central pair microtubules. BMC Biol, 5 Abstract.  Author URL.
Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, et al (2006). Flagellar motility is required for the viability of the bloodstream trypanosome. Nature, 440(7081), 224-227. Abstract.  Author URL.
Dawe HR, Farr H, Portman N, Shaw MK, Gull K (2005). The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis. J Cell Sci, 118(Pt 23), 5421-5430. Abstract.  Author URL.
Dawe HR, Minamide LS, Bamburg JR, Cramer LP (2003). ADF/cofilin controls cell polarity during fibroblast migration. Curr Biol, 13(3), 252-257. Abstract.  Author URL.
Cramer LP, Briggs LJ, Dawe HR (2002). Use of fluorescently labelled deoxyribonuclease I to spatially measure G-actin levels in migrating and non-migrating cells. Cell Motil Cytoskeleton, 51(1), 27-38. Abstract.  Author URL.

Conferences

Mseka T, Dawe HR, Bamburg JR, Cramer LP (2004). Actin filament depolymerization and microtubules control sequential actin dynamic steps to initiate cell polarity.  Author URL.

Publications by year


In Press

Pratt MB, Titlow JS, Davis I, Barker AR, Dawe HR, Raff JW, Roque H (In Press). Drosophila sensory cilia lacking MKS proteins exhibit striking defects in development but only subtle defects in adults. J Cell Sci, 129(20), 3732-3743. Abstract.  Author URL.

2016

Barker AR, McIntosh KV, Dawe HR (2016). Centrosome positioning in non-dividing cells. Protoplasma, 253(4), 1007-1021. Abstract.  Author URL.
Martin-Urdiroz M, Deeks MJ, Horton CG, Dawe HR, Jourdain I (2016). The Exocyst Complex in Health and Disease. Frontiers in Cell and Developmental Biology, 4 Full text.

2014

Barker AR, Renzaglia KS, Fry K, Dawe HR (2014). Bioinformatic analysis of ciliary transition zone proteins reveals insights into the evolution of ciliopathy networks. BMC Genomics, 15 Abstract.  Author URL.  Full text.
Locke JM, Da Silva Xavier G, Dawe HR, Rutter GA, Harries LW (2014). Increased expression of miR-187 in human islets from individuals with type 2 diabetes is associated with reduced glucose-stimulated insulin secretion. Diabetologia, 57(1), 122-128. Abstract.  Full text.
Barker AR, Thomas R, Dawe HR (2014). Meckel-Gruber syndrome and the role of primary cilia in kidney, skeleton, and central nervous system development. Organogenesis, 10(1), 96-107. Abstract.  Author URL.

2013

Barker AR, McIntosh K, Dawe HR (2013). Meckel-Gruber syndrome proteins are required for actin cytoskeleton organisation and directional cell migration. MOLECULAR BIOLOGY OF THE CELL, 24 Author URL.

2012

Adams M, Simms RJ, Abdelhamed Z, Dawe HR, Szymanska K, Logan CV, Wheway G, Pitt E, Gull K, Knowles MA, et al (2012). A meckelin-filamin a interaction mediates ciliogenesis. Hum Mol Genet, 21(6), 1272-1286. Abstract.  Author URL.
Adams M, Simms RJ, Abdelhamed Z, Dawe HR, Szymanska K, Logan CV, Wheway G, Pitt E, Gull K, Knowles MA, et al (2012). A meckelin-filamin a interaction mediates ciliogenesis. Human Molecular Genetics, 21(6), 1272-1286. Abstract.
Cheng Y-Z, Eley L, Hynes A-M, Overman LM, Simms RJ, Barker A, Dawe HR, Lindsay S, Sayer JA (2012). Investigating embryonic expression patterns and evolution of AHI1 and CEP290 genes, implicated in Joubert syndrome. PLoS One, 7(9). Abstract.  Author URL.
Simms RJ, Hynes AM, Eley L, Inglis D, Chaudhry B, Dawe HR, Sayer JA (2012). Modelling a ciliopathy: Ahi1 knockdown in model systems reveals an essential role in brain, retinal, and renal development. Cellular and Molecular Life Sciences, 69(6), 993-1009. Abstract.
Simms RJ, Hynes AM, Eley L, Inglis D, Chaudhry B, Dawe HR, Sayer JA (2012). Modelling a ciliopathy: Ahi1 knockdown in model systems reveals an essential role in brain, retinal, and renal development. Cell Mol Life Sci, 69(6), 993-1009. Abstract.  Author URL.
Thompson H, Shaw MK, Dawe HR, Shimeld SM (2012). The formation and positioning of cilia in Ciona intestinalis embryos in relation to the generation and evolution of chordate left-right asymmetry. Dev Biol, 364(2), 214-223. Abstract.  Author URL.

2011

Vaughan S, Dawe HR (2011). Common themes in centriole and centrosome movements. Trends Cell Biol, 21(1), 57-66. Abstract.  Author URL.  Full text.

2010

Gluenz E, Höög JL, Smith AE, Dawe HR, Shaw MK, Gull K (2010). Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans. FASEB J, 24(9), 3117-3121. Abstract.  Author URL.

2009

Dawe HR, Adams M, Wheway G, Szymanska K, Logan CV, Noegel AA, Gull K, Johnson CA (2009). Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton. J Cell Sci, 122(Pt 15), 2716-2726. Abstract.  Author URL.  Full text.
Dawe HR, Gluenz E (2009). The tale of the trypanosome tail. Biologist, 56(4), 216-220.

2007

Dawe HR, Farr H, Gull K (2007). Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells. J Cell Sci, 120(Pt 1), 7-15. Abstract.  Author URL.
Dawe HR, Smith UM, Cullinane AR, Gerrelli D, Cox P, Badano JL, Blair-Reid S, Sriram N, Katsanis N, Attie-Bitach T, et al (2007). The Meckel-Gruber Syndrome proteins MKS1 and meckelin interact and are required for primary cilium formation. Hum Mol Genet, 16(2), 173-186. Abstract.  Author URL.
Dawe HR, Shaw MK, Farr H, Gull K (2007). The hydrocephalus inducing gene product, Hydin, positions axonemal central pair microtubules. BMC Biol, 5 Abstract.  Author URL.

2006

Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, et al (2006). Flagellar motility is required for the viability of the bloodstream trypanosome. Nature, 440(7081), 224-227. Abstract.  Author URL.

2005

Dawe HR, Farr H, Portman N, Shaw MK, Gull K (2005). The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis. J Cell Sci, 118(Pt 23), 5421-5430. Abstract.  Author URL.

2004

Mseka T, Dawe HR, Bamburg JR, Cramer LP (2004). Actin filament depolymerization and microtubules control sequential actin dynamic steps to initiate cell polarity.  Author URL.

2003

Dawe HR, Minamide LS, Bamburg JR, Cramer LP (2003). ADF/cofilin controls cell polarity during fibroblast migration. Curr Biol, 13(3), 252-257. Abstract.  Author URL.

2002

Cramer LP, Briggs LJ, Dawe HR (2002). Use of fluorescently labelled deoxyribonuclease I to spatially measure G-actin levels in migrating and non-migrating cells. Cell Motil Cytoskeleton, 51(1), 27-38. Abstract.  Author URL.

helen_dawe Details from cache as at 2018-09-19 00:38:26

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Editorial responsibilities

Reviewer for Journal of Cell Science, Journal of Cell Biology, Human Molecular Genetics


Invited lectures

2011 Marie Curie Regenerative medicine symposium, Bath

2008 1st EMBO Conference on Centrosomes and Spindle Pole Bodies

2007  FASEB Summer Research Conference “The Biology of Cilia and Flagella”

Given invited seminars at 14 UK universities & research institutes

Year 2
BIO2088 Advanced Cell Biology

Year 3
BIO3077 Frontiers in Molecular Cell Biology

Research Fellows

  • Amy Barker

Postgraduate researchers

Alumni

  • David Pitcher
  • Helen Thompson DPhil student, University of Oxford

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