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Dr Michelle Hares   (nee Pound )

Dr Michelle Hares (nee Pound )

Project Manager for Estates, logistics & Infrastructure (Postdoctoral Research Fellow)

 01326 259404

 Peter Lanyon A042

 

Peter Lanyon Building, University of Exeter, Penryn Campus, Treliever Road, Penryn, Cornwall, TR10 9FE, UK

Overview

I am Project manager for major capital projects on Cornwall campuses for the University of Exeter. 
Within this role I ensure smooth end to end completion of projects by;
- Developing, writing and co-ordinate funding bids both within the University and external agencies with UoE client.
- Agree project objectives with stakeholders, the project board and any other relevant parties
- Represent client side interests through project life-cycle, providing operational advice and supporting project boards and other internal governance infrastructure.
-Stakeholder management including developing communications plans and customer occupation
To date I have successfully project managed the Capital bid and project delivery of the University of Masters Suite facility on Penryn campus worth £1.2 million and the £ 1.77 million Renewable Energy Engineering Facility (REEF)
Current projects include;
- £12 million Science and Engineering Research Support Facility (SERSF)Extension
I also Project manage small infrastructure project delivery on Penryn Campus.

In addition as UoE representative for Health, Safety, Welfare, Accessibility, Business Continuity and Sustainability on Penryn campus; deputising for Head of Professional services where applicable.

Qualifications

PhD Investigation into the insecticidal Toxin complexes from Photorhabdus luminescens and Yersinia
MPhil Deletion screening and haplotype analysis in the FRAXE region at Xq28
BSc (Hons) Biochemistry

Career

I first Joined the University of Exeter in 2006 as  research Technician for Richard ffrench-Constant, while completing a PhD researching the mechanisms and evolution in bacterial TC toxins. After my PhD I secured a permenant position as an Experimental officer for the Centre for Ecology & Conservation. Within this role, I worked with academic colleagues to develop  the reseach laboratories to support  new tools for research such as cell biology and molecular biology. I also carried out R&D pilot studies to provide preliminary data to support grant applications.  Subsequently I was promoted to Senior Laboratory manager, managing the operation of 10 research laboratories within CLES. This was in tandem to fundend research with academic colleagues in CLES. During this time, I was lucky enough to have a sabatical as a lecturer within CLES where I developed and taught Approaches to Evolutionary and Behavioural Ecolgy module as part of the MSc Evolution and Behavioural Ecology. I also spent a year teaching the UG module Developmental Biology and Diversity.

Subsequently I was asked to represent CLES requirements to develop the design of the Biological research facilites in the ESI building. This lead me to my new role as Project Manager; Estates, Logistics and Infrastructure. Within this roles I project manage both capital building projects and refurbishment projects on Penryn Campus.   

Research group links

Research

Research interests

.

I am interested in ageing and telomere dynamics and how differences in telomere length and telomerase activity have co-evolved with fitness traits in wild populations of animals.

Research projects

Telomere Dynamics and life history in wild populations

Currently, I am working with Mike Cant and Jon Blount as a postdoctoral Research Fellow to investigate telomere length using QPCR in the banded mongoose. Banded Mongoose are highly cooperative and are unusual as females from the same mate group give birth to offspring on the same day. Interestingly, offspring are cared for by non-breeding males and this care varies. We are investigating whether there is a cost with respect to telomere length, in offspring who have high levels of care compared to those who have low level care.

Recent Publications

Hares MC, Vitikainen EIK, Marshall HH, Thompson FJ, Blount JD, Cant MA. (2018) Telomere dynamics in wild banded mongooses; Evaluating Longitudinal and quasi longitudinal markers of senescence. Exp Gerontol. 107:67-73. 

Marshall HH, Vitikainen EI, Mwanguhya F, Businge R, Kyabulima S, Hares MC, Inzani E, Kalema-Zikusoka G, Mwesige K, Nichols HJ, Sanderson JL, Thompson FJ, Cant MA. (2017) Lifetime fitness consequences of early-life ecological hardship in will mammal population. Ecol Evol. 7(6):1712-1724. .

Marshall HH, Sanderson JL, Mwanghuya F, Businge R, Kyabulima S, Hares MC, Inzani E, Kalema-Zikusoka G, Mwesige K, Thompson FJ, Vitikainen EI, Cant MA. (2016) Variable ecological conditions promote male helping by changing banded group composition. Behav Ecol. 27(4):978-987. 

Beirne C, Delahay R, Hares M, Young A. (2014) Age-related declines and disease-associated variation in immune cell telomere length in a wild animal. PLoS One 9:e108964.

Harrison X. A, York J. E, Cram D. L, Hares M. C, Young A. J. (2013) Complete Reproductive Skew within White-browed Sparrow Weaver Groups Despite Outbreeding Opportunities for Subordinates of Both Sexes. Behav Ecol Sociobiol 69 (12) 1997-2004

 

Evolution of bacterial toxins

I previously carried out research with Richard ffrench-Constant investigating bacterial pathogens and how they interact with their host to illicit their effects. I am particularly interested in the toxins bacteria use to invade their host and how homologous toxins from different bacteria have evolved to target different hosts. In particular, the evolution of the bacterial toxins of Photorhabdus. In particular, I am interested in the Toxin complexes (Tc).

The Tc genes were first identified in the insect pathogen Photorhabdus luminescens strain W14. These high molecular weight proteins (~1MDa) have been shown to be orally and injectably toxic to several orders of insects and represent a novel bio-insectide. They are encoded by four loci; tca, tcb, tcc and tcd, the genes within these loci labeled according to their order ( tcdA, tcbB, tccC) (Waterfield et al 2001). Significant homology is observed between the four loci and previous work has shown that three components are required for full toxicity towards the model insect pest Manduca sexta, the tcdA-like [A], the tcbB-like [B] and tccC-like [C] genes Waterfield, Hares et al 2005).

Subsequently these Tc’s have been identified in a variety of gram-negative pathogenic bacteria including the mammalian pathogens Yersinia pestis and Y. pseudotuberculosis, suggesting an evolving function or targets for these proteins directed towards insect and /or mammalian hosts (Waterfield, Hares et al 2007).

Indeed, Y. pestis Tc or Y. pseudotuberculosis Tc, which are mammalian pathogens, showed no toxic effects when fed to the model insect host Manduca sexta. However, we have shown that when the Y. pseudotuberculosis Tc’s are applied to the mammalian gut cell line CACO2 membrane ruffling, vacuolation and multinucleation is induced, whereas Tc gene knockouts were comparable to the control. These results were recapitulated when heterologously expressed ABC were applied to CACO2 cells or when individual Tc genes were transfected into CACO2 cells. This suggests the Tc toxins have adapted to their hosts. It is yet to be determined whether this is due to a change in function or change in host target (Hares, Hinchliffe et al 2008). Further compounding evidence for evolutionary adaptation of the Tc’s has been demonstrated in the insect pathogen Y. entomophagus. Unlike Y. pestis and Y. pseudotuberculosis this insect pathogen, however, like Y. pestis and Y. pseudotuberculosis is has been shown to have similar phenotypic effects on host cells (Marshall, Hares et al 2012). This suggesting that it is changes in host specificity rather than mode of action that have been adapted.

Research networks

University of Exeter
Dr Michael Cant
Dr Jon Blount
Dr Andrew Young
Professor Richard ffrench-Constant
Professor Brendan Godley

Others

Dr Nick Waterfield, University of Bath
Dr Mark Hurst, AgResearch, New Zealand

Publications

Key publications | Publications by category | Publications by year

Key publications


Hares MC, Hinchliffe SJ, Strong PCR, Eleftherianos I, Dowling AJ, ffrench-Constant RH, Waterfield N (2008). The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is active against cultured mammalian cells. Microbiology, 154(Pt 11), 3503-3517. Abstract.  Author URL.

Publications by category


Journal articles

Hares MC, Vitikainen EIK, Marshall HH, Thompson FJ, Blount JD, Cant MA (2018). Telomere dynamics in wild banded mongooses: Evaluating longitudinal and quasi-longitudinal markers of senescence. Exp Gerontol, 107, 67-73. Abstract.  Author URL.  Full text.
Marshall SDG, Hares MC, Jones SA, Harper LA, Vernon JR, Harland DP, Jackson TA, Hurst MRH (2012). Histopathological effects of the Yen-Tc toxin complex from Yersinia entomophaga MH96 (Enterobacteriaceae) on the Costelytra zealandica (Coleoptera: Scarabaeidae) larval midgut. Appl Environ Microbiol, 78(14), 4835-4847. Abstract.  Author URL.
Hinchliffe, SJ, Hares MC, Dowling, AJ, ffrench-Constant, RH (2010). Insecticidal toxins from the Photorhabdus and Xenorhabdus bacteria. The Open Toxicology, 83-100.
Hares MC, Hinchliffe SJ, Strong PCR, Eleftherianos I, Dowling AJ, ffrench-Constant RH, Waterfield N (2008). The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is active against cultured mammalian cells. Microbiology, 154(Pt 11), 3503-3517. Abstract.  Author URL.
Dowling AJ, Waterfield NR, Hares MC, Le Goff G, Streuli CH, ffrench-Constant RH (2007). The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain. Cell Microbiol, 9(10), 2470-2484. Abstract.  Author URL.
Waterfield N, Hares MC, Hinchliffe S, Wren B, ffrench-Constant R (2007). The insect Toxin complex of Yersinia. Ad Exp Med Biol, 603, 247-257.
Waterfield N, Hares MC, Yang G, Dowling A, ffrench-Constant R (2005). Potentiation and cellular phenotype of the insecticidal Toxin complex of Photorhabdus bacteria. Cell Microbiol, 7, 373-382.
Holloway DE, Chavali GB, Hares MC, Subramanian V, Acharya KR (2005). Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies. Acta Crystallogr D Biol Crystallogr, 61(Pt 12), 1568-1578. Abstract.  Author URL.
Holloway DE, Chavali GB, Hares MC, Baker MD, Subbarao GV, Shapiro R, Acharya KR (2004). Crystallographic studies on structural features that determine the enzymatic specificity and potency of human angiogenin: Thr44, Thr80, and residues 38-41. Biochemistry, 43(5), 1230-1241. Abstract.  Author URL.
Waterfield N, Daborn P, Dowling A, Yang G, Hares MC, ffrench-Constant R (2003). The insecticidal toxin Makes Caterpillars floppy 2 (Mcf2) shows similarity to HrmA, a virulence protein from a plant pathogen. Fems Microbiol Letts, 229, 265-270.
Holloway DE, Shapiro R, Hares MC, Leonidas DD, Acharya KR (2002). Guest-host crosstalk in an angiogenin-RNase a chimeric protein. Biochemistry, 41(33), 10482-10489. Abstract.  Author URL.
Holloway DE, Hares MC, Shapiro R, Subramanian V, Acharya KR (2001). High-level expression of three members of the murine angiogenin family in Escherichia coli and purification of the recombinant proteins. Protein Expr Purif, 22(2), 307-317. Abstract.  Author URL.

Chapters

ffrench-Constant R, Dowling A, Hares MC, Yang G, Waterfield N (2007). Photorhabdus: Genomics of a Pathogen and Symbiont. In Pallen MJ, Nelson KE, Preston GM (Eds.) Bacterial Pathogenomics, Washington D.C.: ASM Press, 419-439.

Publications by year


2018

Hares MC, Vitikainen EIK, Marshall HH, Thompson FJ, Blount JD, Cant MA (2018). Telomere dynamics in wild banded mongooses: Evaluating longitudinal and quasi-longitudinal markers of senescence. Exp Gerontol, 107, 67-73. Abstract.  Author URL.  Full text.

2012

Marshall SDG, Hares MC, Jones SA, Harper LA, Vernon JR, Harland DP, Jackson TA, Hurst MRH (2012). Histopathological effects of the Yen-Tc toxin complex from Yersinia entomophaga MH96 (Enterobacteriaceae) on the Costelytra zealandica (Coleoptera: Scarabaeidae) larval midgut. Appl Environ Microbiol, 78(14), 4835-4847. Abstract.  Author URL.

2010

Hinchliffe, SJ, Hares MC, Dowling, AJ, ffrench-Constant, RH (2010). Insecticidal toxins from the Photorhabdus and Xenorhabdus bacteria. The Open Toxicology, 83-100.

2008

Hares MC, Hinchliffe SJ, Strong PCR, Eleftherianos I, Dowling AJ, ffrench-Constant RH, Waterfield N (2008). The Yersinia pseudotuberculosis and Yersinia pestis toxin complex is active against cultured mammalian cells. Microbiology, 154(Pt 11), 3503-3517. Abstract.  Author URL.

2007

ffrench-Constant R, Dowling A, Hares MC, Yang G, Waterfield N (2007). Photorhabdus: Genomics of a Pathogen and Symbiont. In Pallen MJ, Nelson KE, Preston GM (Eds.) Bacterial Pathogenomics, Washington D.C.: ASM Press, 419-439.
Dowling AJ, Waterfield NR, Hares MC, Le Goff G, Streuli CH, ffrench-Constant RH (2007). The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain. Cell Microbiol, 9(10), 2470-2484. Abstract.  Author URL.
Waterfield N, Hares MC, Hinchliffe S, Wren B, ffrench-Constant R (2007). The insect Toxin complex of Yersinia. Ad Exp Med Biol, 603, 247-257.

2005

Waterfield N, Hares MC, Yang G, Dowling A, ffrench-Constant R (2005). Potentiation and cellular phenotype of the insecticidal Toxin complex of Photorhabdus bacteria. Cell Microbiol, 7, 373-382.
Holloway DE, Chavali GB, Hares MC, Subramanian V, Acharya KR (2005). Structure of murine angiogenin: features of the substrate- and cell-binding regions and prospects for inhibitor-binding studies. Acta Crystallogr D Biol Crystallogr, 61(Pt 12), 1568-1578. Abstract.  Author URL.

2004

Holloway DE, Chavali GB, Hares MC, Baker MD, Subbarao GV, Shapiro R, Acharya KR (2004). Crystallographic studies on structural features that determine the enzymatic specificity and potency of human angiogenin: Thr44, Thr80, and residues 38-41. Biochemistry, 43(5), 1230-1241. Abstract.  Author URL.

2003

Waterfield N, Daborn P, Dowling A, Yang G, Hares MC, ffrench-Constant R (2003). The insecticidal toxin Makes Caterpillars floppy 2 (Mcf2) shows similarity to HrmA, a virulence protein from a plant pathogen. Fems Microbiol Letts, 229, 265-270.

2002

Holloway DE, Shapiro R, Hares MC, Leonidas DD, Acharya KR (2002). Guest-host crosstalk in an angiogenin-RNase a chimeric protein. Biochemistry, 41(33), 10482-10489. Abstract.  Author URL.

2001

Holloway DE, Hares MC, Shapiro R, Subramanian V, Acharya KR (2001). High-level expression of three members of the murine angiogenin family in Escherichia coli and purification of the recombinant proteins. Protein Expr Purif, 22(2), 307-317. Abstract.  Author URL.

michelle_hares Details from cache as at 2018-12-14 06:01:00

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Teaching

I developed and ran the module; Approaches in Evolution and Behavioral Ecology within the Evolution and Behavioral Ecology Masters Programme from 2009-2013 coordinated by Professor Nina Wedell.

In 2011 I also taught UG module Development and Biodiversity.

Outreach activities included supporting A level student currium by providing experimental laboratory experients for students from  Redruth School.

I also ran University Laboratory taster days in Conservation genetics for local secondary schools.

I have also been guest speaker at Falmouth School discussing the merits and future of DNA sequencing technology and genetics with Biology A level students.

 

 

Modules

2018/19

Information not currently available


Supervision / Group

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