Overview

Qualifications

BSc (Exon) (Hons) Biological Sciences, University of Exeter

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Research

Research projects

Project Title: The potential health effects of nanoparticles on fish

Supervisors: Professor Charles Tyler, Dr. Tetsuhiro Kudoh and Professor Tamara Galloway

Funding Body: NERC and Environmental Agency

Project Description:

My research is looking into the ecotoxicology of nanoparticles and their potential effects in fish. So far, I have investigated the uptake and effects of metal oxides nanoparticles in zebrafish embryos using a variety of end points: acute toxicity methods, apoptosis assays, morphological differences and Coherent Anti Raman Scattering imaging technique. I have also focused on zebrafish early life stages, and the application of molecular techniques; specifically adopting an integrative approach for identifying gene target tissues and effect mechanisms of metal/metal oxide particles using the in-situ hybridization procedure. My latest work entails an in vivo breeding exposure study via the diet of zebrafish to determine if nanoparticles can impact on the breeding of zebrafish (Danio rerio) and undergo maternal transfer with biological consequences for the subsequent offspring.

Publications/Presentations:

Research Day June 2010 at the Peninsula College of Medicine and Dentistry
Poster Presentation: Investigating the Biological effects of Engineered Nanoparticles in a Fish Model

Microscopy & Analysis Conference January 2011 at the University of Bath
Poster Presentation: Investigating the Biological effects of Engineered Nanoparticles in a Fish Model

6th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials at the Royal Society, London September 2011
Oral Presentation:  Effects of particle size and coating on nanoscale Ag and TiO2 exposure in zebrafish embryos (Danio rerio)

7th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials ,The Banff Centre, Banff, AB Canada, September 2012
P Presentation: Application of in situ hybridization in zebrafish to identify target tissues and effect mechanisms of a metal based nanoparticle (silver)

8th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials ,Aix en Provence, France, July 2013
Poster Presentation: Application of in situ hybridization in zebrafish to identify target tissues and effect mechanisms of a metal based nanoparticle (silver)

8th European Zebrafish Conference, Barcelona, July 2013
Oral Presentation: Application of in situ hybridization in zebrafish to identify target tissues and effect mechanisms of a metal based nanoparticle (silver)

Osborne, O.J., Johnston, B., Moger, J., Baalousha, M., Lead, J., Kudoh, T., Tyler, C. (2012) Effects of particle size and coating on nanoscale Ag and TiO2 exposure in zebrafish (Danio rerio) embryos. Nanotoxicology doi:10.3109/17435390.2012.737484

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